Title Mupid-3を用いたアガロースゲル電気泳動法による乳酸
Contents
1. Mupid 3 3 1 2 250mmolL 125mmolL Mupid 3 32. 2 Mupid 3 Mupid 3 LD
3. 1 x0 2cm Mupid LD 125mmolL pHS8 6 1 5 S 0 5cm 100V 30 40 EZL RERNE e RHE 37C 40 1 Mupid 3 31 2 81 83 2007 KERF 32 2 49 51 2008 3 Mupid 3
4. 300ml 1 10 20 1 100V 30 45 5 LD 37C 40 1 1 125mmol L 250mmol L 1 0 1 25 1 5 7 1 5 1 0 1 25 125mmol L k Y 250mmol L
5. LD LD 7 LD 0 3 0 5cm 5 LD 5 LD
6. 2 E 1 125mmol L pH8 6 15 15g 1 molL 11 6 25ml 1000ml 2 250mmol L pH8 6 30 29g 1 mol L 11 6 50ml 1000ml 3 200mmol L pH8 6 2 4 200mmol L pH8 0 200mmol L 27 2g L 200mmol L 12 71 6g L pH 8 0 5 0 1mol L 96mg L 10ml 6 4mg ml 3 4 5 2 2 5 2H MTT MTT 40mg 3 4 O 10ml 5 10C 7 7 0 2mg ml
7. 5 mg 25ml 8 0 1molL 10ml NAD 10mg 4 mg ml MTT 0 7ml 0 2mg ml 0 1ml 200mmolL pH80 200mmolL pH8 6 4 0ml 15ml 3 1 0 S 150mg 50ml 15ml 30 40 70C 2 3 52x60mm 70 C S 1 25 1 5 S 188mg 225mg 1 0 4 Mupid 3 3
8. F LD LD 4 LD 5 N AD pH 9 pH 8 0 pH8 6 pH pH8 0 3 Mupid 3 1 1 5 2 137 BT Il A y Ts 1 1 t 1 LD5 LD4 LD3 LD2 LD1 B N PB M jJ N N jp n Tris IN N N V N A A N i k 2 LD A LD PB
9. 125mmolL 136 Mupid 8 250mmol L 1 5 2 125mmol 250mmolL 100V 40 Mupid 3 10C 250mmol L 28 5 C 125mmolL 19 3 C LD 125mmol L 250mmol L LD 1 LD 4 LD 5 125mmol L 250mmol L 125mmol L
10. 4 ea GE LDH 1 19 627 632 1971 5 28 pp MI130 WW132 1978 6 pp281 289 1999 7 Medical Technology 7 pp1169 1173 1979 138 Mupid 3 An examination of separation conditions for lactate dehydrogenase isozymes by agarose gel electrophoresis using Mupid 3 Keiko Homma Yoko Mita Chieko Fujikawa Maki Nishitani Kazuhiro Mawatari 139
11. pH8 0 Tris p H8 6 B A LD5 LD4 LD3 LD2 LD1 L D 4 125mmolL 3 4 5 0 2cm LD
12. 100V 30 40 2 1 5 125 mmol L 100V40 2 2 2 A B T H J 1 H Z 2 L 3 1 4 1 LD5 LD3 LD1 LD4 LD2 1 LD 100V 30 125mmol L pH8 6 pH8 0 0 06 F pH8 6
13. 125mmol L SEELS 3 125mmol L 100V 30 45 1 30 LD 1 4 LD 5 LD 4 45 LD 5 40 LD5 LD 1 LD 5 LD 1 LD5 LD
14. KURA Kanazawa University Renasitory for Academie resources ERAFFMRRUNIN gt KATNAZ AW A Tite Mupid3 000000000000000 0000000000 IIHHHHHHHHHHHH Author s 00 00 00 00 00 000 00 00 00 00 CAO Journa oftheTsuuma Health Science Society Kanazawa University 34 2 135 139 Issue Date 2011 01 04 Type Departmental Bulletin Paper Text version publisher URL http ndl handle net 2297 26223 Right KURAHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH KURAHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHH htp dspace IiD kanazawa U aC jD dsDace Vol 34 2 135 139 2010 Mupid 8 AT Key words Electrophoresis tris HCl buffer lactate dehydrogenase isozymes Mupid 3 agarose gel
15. Mupid 3 9 0 5cm Ue 135 BT 4 LD Mupid 3 LD LD 1 0 3ml 20C
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