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1. 336 340nm 3 336 lt 340nm 3 10 CV i 3 10 CV 5 10 200 up mol L 2 Williamson _ 200 _ 200 3 g g 150 150 0 100 K 48 K n 44 r 0 985 r 0 987 3 50F y 1 23x 0 3 50 y 1 27x 0 1 0 0 12. wn a 2 2013 4 4 877439 2007 4 3 16200AMZ00438000 35mLX A 0mLX 1 B 8mLX 1 A 4mL X2 B 8mL X
3. Energy charge NAD Iink NAD NADH NAD7NADH 3 3 3 AKBR Schl
4. 3 1 2 8C 2 1 6 30 1 Williamson DH Mellanby J Krebs HA Enzymic determination of D B hydroxybutyric acid and acetoacetate in blood Biochem J 82 90 1962 2 Uno sg Ito T Kurono M Yamaoka Y Kamiyama Y Ozawa K A simple and sensitive assay for blood ketone bodies using highly purified 3 hydroxybutyrate dehydrogenase Clin Chim Aota 168 253 1987 3 Williamson DH Lund P Krebs HA The redox state of free nicotinamide adenine dinucleotide in the cytoplasm and mitochondria of rat liver Biochem J 103 514 1967 4 Lehninger AL Sudduth HC Wise JB D 8 Hydroxybutyric dehydrogenase of mitochondria J Biol Chem 235 2450 1960 5 Schlichtig R Klions HA Kramer DJ Nemoto EM Hepatic dysoxia commences during 02 supply dependence J Appl Physiol 72 1499 1992 6 0zawa K Chance B Tanaka A Iwata S Kitai T lIkail Linear corre
5. B 0 25mL 37C 5 340nm 1 Eo Eo B 005mL 0 05mL 37C 15 340nm 2 EfA Ef B 3 Lu mol L 075_ 0 80x2 Eo XTgg FTO0X Sx103xX1 0xX0 50 075_ Eo A Xs ET 001JX 514 3 umol L 075_ 0 80x2 EB Eo B XT gg 0 01JX S55x103x1 0x0 50 _ 075_ Ef B Eo B xX 3a 001JX514 3 075 Eo A x Ef A 001 0 75 Ef B Eo B X580 0 01 0 80 mL 0 75 mL 6 22X103 NADH 0 50 mL 1 0 Cm 2 0 01 3 ATP
6. FAX 052 950 1305 461 8631 35 TEL 052 951 8130 SKK 35 461 8631 MB 11
7. 3 7mLX 1 3 Williamson 9 NADH H 3 NAD NADH 340nm 3 3 HBDH 3 2 1 3 3 4 10 800 umol L 1 1 20C 2 2 8 48 3 2 1
8. 3 10urmol L 10 nmol L 10umol L CR 10 200u mol L 1 1 2 CR 3 A B 2 8 4 3 100mg dL 161mg dL 5000mg dL 600g dL 10000mg dL 200mg dL 82800mg
9. 1 0 50 100 150 200 50 100 150 200 Williamson zmol L Williamson mol L Williamson 3 1 1 HB 2 8Q 8 3
10. dL 1 1 A A 1 4mL A C 8C 10 2 B B 1 B 1 B 28C 0 3 1 1 5mL 2 8C 30 2 1 1 mL 1 mL 10 4 3000rpm 10 A B 3 0 50mL 0 50mL A 0 25mL
11. ichtig Ketone extraotion ratio Ozawa Chance Redoxs scanner 9 Kitai 3 P NMH B ATP Pi2 Ketogenesis AKBRH Oxi max Energy charge AKBR AKBR 1 1 90 110 3 90 110
12. lation between acetoacetate hydroxyDutyrate in arterial blood and oxidized flavOprotein reduced pyridine nucleotide in freezetrapped human liver tissue Biochem Biophys Aota 1138 350 1992 9 Kitai T Tanaka A Terasaki M Okamoto R Ozawa K Morikawa S InuDushiT Energy metabolism of the liver in brain dead dog assessed by 31P_NMR spectroscopy gnd arterial ketone Dody ratio Life Sci 45 511 1991 8 0zawa K Aoyama H Yasuda K Shimahara Y Nakatani T Tanaka J Yamamoto M Kamiyama Y and Tobe T Metabolic abnormalities associated with postoperative Organ failure a redox theory Arch Surg 118 1245 1983 9 0zawa K Liver surgery approached through the mitochondria The redox theory in evolution Karger Basel 1992 10 Asonuma K Takaya S Selby R Okamoto R Yamamoto Y Yokoyama T Todo S Ozawa K and Starz TE The clinical significance of the arterial ketone body ratio as gan early indicator of graft viaDility in human liver transplantation Transplantation 51 163 1991 11 Ozawa K Living related donor liver transplantation Assessment of graft viability based on the redox theory Karger Basel 1994 1 Dalton N Arterial Ketone Body Ratio The Importance of 1 i Standard Liver Tests European Clinical Laboratory 13 6 14 1994 XI 0120 19 8130 9 00 17 00
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