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1. 1 lt 83 1 2 3 TRCRapid 160 MAC2. 3 1 2 3 1 2 20C 12 0053002 MAC rRNA TRCRapid MAC 48 1 Ishiguro T et al Intercalation activating fluorescerce DNA probe and its application to homo
3. CDC NALC NALC 1 15 mL 1 mL 2 2 2 NALC NaOH 296 NALC NaOH 4 NaOH 0 1 mol L 50 mL N Acety L Cystein 0 5 g 3 5 25 15 4 0 067 mol L pH 6 8 15 mL 5 4 3 000xg 20 6 0 067 mol L pH 6 8 1 mL EXTRAOEN MB 0054001
4. EXTRAGEN MB EXTRAGEN MB 1 1 5 mL EXTRAGEN MB 1 mL 2 200 5 3 70C 3 4 16000Xg 4 5 5 EXTRAGEN MB 50 nL 24 kHz 31 kHz 2 5 6 16000Xg 4 3 5 pL 2 McFarland No 0 5 600nm 0 1 1 10000 5 mg mL 0 1 mg mL BDTA 10 mg mL
5. 2 MM avium M intracellulare 3 610 nm 2 610 nm 1 2 TRCR F 47 kansasii MM ggs7
6. MAC rRNA RNA MAC rRNA 520 nm 1 610 nm 2 MAC 16S rRNA 30 1 2 3
7. 3 2 1 2 3 4 20C 1 5 16 6
8. 3 6 1 2 TRCRapid 160 gt 4 TRCR PC 1 30 520 nm 1 1 3 2 30 520 nm 1 1 3 1
9. 4 5 1971 0 79 2001 24 5 40 19 MAC 70 809 MAC MAC MAC 4 8 MAC
10. Mycobacterium avium complex rRNA Je DNA RNA RNA 1 TRC Transcription Reverse transcription Concerted reaction RNA NTP dNTP ITP 7 aw MM mntracellulare MAC rRNA mm mum MAC rRNA gt 1 MAC rRNA 1 2 a 2 cDNA MAC FRNA es af i DNA RNA es
11. TRC 1 MAC RNA rRNA 30 1 1 1 1 1 30 520 nm 1 3 2 10 5 30 520 nm 1 3 3 10 5 iL 30 520 nm 1 3 1 gp MM mtracellafare 16S rRNA 2 rRNA DNA
12. 20 pL 3 4 3 5 LX e 1 20C 5 6 1 43 470 nm 520 nm 610 nm TRCR TRCRapid 160 2 Ey 5 200 mL RNase 200 pL ART No 2069 20 ART No 2149P 4 0 5 mL DNA LoBind Tube Eppend
13. RNA RNA 3 7 intracellulare rRNA DNA RNA RNA 1 7 avium 7 intracellulare 16S rRNA 2 RNA 10 5 pL 3 RNA 10 5 uL 3 1 3 2 3 3 3 1 2 TRCR TRCRapid 160 1
14. RNA 1000 RNA 1000 1 rRNA 2 MAC 1 161 140 12 6 1 1 1 24 22 PCR 91 3 147 161 1009 Il la 1 1 6 2 1 6 2
15. Enzyme Position EP 3 4 1 2 5 3 4 1 3 4 5 pL 1 TRCRapid 160 EP 5 pL SP 4 5 6 2 TRCRapid 160 SP SP START START TRCRapid 160 SP START 3 1 2 TRCRapid 160 SP1 SP8
16. DU DU 5uL 5 nuL 5uL 5 uL TRCR TRCRapid 160 5 TRCRapid 160 2 5 pL TRCRapid 160 30 TRCR TRCRapid EXTRAGEN 252 1123 2743 1 0120 17 1200 TEL 0467 76 5384 FAX 0467 79 2550 105 8623 3 8 2 TEL 03 5427 5181 CD TOSOH
17. 10 mg mL DNA 0 1 mg mL 15 25 1 1 2 10 uL c 6 20C 3
18. RNA s ta uh th MAC rRNA MAC rRNA RNaseH MAC rRNA 5 MAC rRNA 1 2 MAC rRNA cDNA MAC rRNA DNA polymerase DNA RNA RNA RNA RNA MAC rRNA Mycobacterium avium complex MAC 16S rRNA RNA RNA DNA RNA
19. 1 dNTP dATP dGTP dCTP dTTP 2 NTP ATP GTP CTP UTP 3 ITP 180 pLX3 1 MAC rRNA 5 CCGCCAGCGTTCGTCCTGAGCCAG 3 2 MAC rRNA 5 AATTCTAATACGACTCACTATAGGG AGATGGCCGGCGTGCTTAACACATGCA 3 3 MAC rRNA 1 5 GACATGCGTCTTGAGG 3 4 MAC rRNA 2 5 TAAAGACATGCGCCTAAA 9 5 MAC rRNA 5 CAAATTGCCCACGTGTTA 3 ENZYME 120 nLx3 1 2 RNA POSITIVE CONT 100 LX3 NEGATIVE CONT 1 Mintracellulare rRNA 2 RNase 100 pLX3 1 RNase
20. 1 2 1 5 5 LL 3 3 TRCRapid 160 1 2 OK Sample Position SP 3 TRCRapid 160 SP1 3 4 1 5 TRCRapid 160 4 TRCRapid 160
21. 5 TRCRapid 160 SP1 SP8 1 2 NG 160 SP9 SP16 5 TRCRapid 160 SP9 SP16 SP1 SP8 1 3 5 1 3 4 30 2 PC
22. 2013 10 5 2011 05 4 MAC rRNA TRCRapid MAC RCR TRCRapid 160 W SUBSTRATE PRIMER 180 LX3 180 pL GTP 608 ng ITP 1055 pg
23. 1 161 24 DNA 92 4 171 185 2 47 zu 2 4 12 1 1 7 3 M avium M intracellulare 16S rRNA DNA RNA 1 1 MAC HIV HBV HCV 2
24. geneous quantification of a target sequence by isothermal sequence amplification in a closed vessel Analytical Biochemistry 314 77 86 2003 2 TRC RNA 206 479 483 2003 3 688 61 2 2003 4 Mycobacterium avium complex IRYO 50 607 615 1996 5 2003 78 569 572 2003 TRCRapid 160 lt lt 20 pL
25. orf No 0030 108 035 5 TRCRapid 160 TRCR 0021030 6 STERLING NITRILE Kimberly Clark 7 8 5000 rpm a 3 3 1 TRCR TRCRapid 160 TRCRapid 160 1 30 43 2 PC TRCR 3 4 ID MAC 0 3 2
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