第3章 調査研究・報告
Contents
1. PFGE PFGE 2 I PFGE2. PFGE Variable Number Tandem Repeats VNTR MLVA Multiple Locus VNTR analysis IS printing 1 MLVA VNTR Dye Forward primer 5 3 Reverse primer 5 3 Cz MI 1 K3 6 FAM GGCGGTAAGGACAACGGGGTGTTTGAATTG GAACAACCTAAAACCCGCCTCGCCATCG 0 2 K9 PET GCGCTGGTTTAGCCATCGCCTTCTTCC GTGTCAGGTGAGCTACAGCCCGCTTACGCTC 0 2 K25 VIC GCCGGAGGAGGGTGATGAGCGGTTATATTTAGTG GCGCTGAAAAGACATTCTCTGTTTGGTTTACACGAC 0 1 K34 PET GACAAGGTTCTGGCGTGTTACCAACGG GTTACAACTCACCTGCGAATTTTTTAAGTCCC 0 2 2 K17 NED GCAGTTGCTCGGTTTTAACATTGCAGTGATGA GGAAATG
3. IS printing 1 PFGE 2 MLVA 6 3 1 H21 V17 3 IS printing H21 V02 ee pt AH 1S printing 2 4 3 PFGE 3 4 2
4. 1 mval kg 10 Cation Anion 100 eq EC 30 S cm 8 R 8 9 R 99 1 EC 4 C Kohlrausch Debye HGckel Onsager Debye Htickel
5. R 11 3 1 EC 5 4 Ci a eh oi eae Kohlrausch R 152 83 9 1 22 3 2 Extended Debye Htckel we NaCl 0 5M 1 0M Extended Debye Htckel R 152 3 9 54 9 Extended Debye H
6. 0 27 2 10 10 2 7 R 3 9 EC 7 20mS cm HaSiO0 HBO HS pH CO HCO CO HCO HCO Kohlrausch
7. Extended Debye Htickel Davies EC EC PC ml 1 EC 2005 30 2 1988 1996 30 EC
8. 0 15 y 0 4129 0 9164X 0 0016 R 0 9970 E010 F 5 1 I 8 3 005 0 000 0 00 Measurement_EC mS cm 4 1 EC gt 2 Ci 0 15 y 0 6153 0 7508x 00023 R 0 9828 CO 1 8 ty 1 8 2 G 0 00 0 05 0 10 0 15 Measurement_EC mS cm 4 2 Extended Debye Huckel 7 WAS OD ROD 1 78 169 174 2007 2 f 28 9 32 1997 3 40 59 64 2008 4 10 105 110 1994 5 Vol 3 No 4 No 393 365 407 2004 po UI 0 10 0 15 6 Craig Bethke Geochemical Reaction Modeling Measurement_EC mS cm p107 114 1996 Oxford University Press
9. 1 B Swaminathan T J Barrett S B Hunter et al Emerg Infect Dis 7 382 389 2001 2 T Ooka J Terajima M Kusumoto et al Microbiol 47 2888 2894 2009 3 Y Pei J Terajima Y Saito et al Jpn J Infect Dis 61 58 64 2008 4 T Sakai H Ohmae and Y Kitahori Jpn J Infect Dis 63 217 219 2010 5 T Sakai S Tanabe M Hashida et al Dis 63 152 153 2010 Clin Jpn J Infect
10. 3 K9 AAATAG 1 AAATAT K36 TCACACC 1 TCACAAC 1 4 PFGE MLVA 1S printing 3 4 RS 9 a 36 PCR 9 SD EN 4 VNTR_10 VNTR 9 3
11. 0 10 mS cm 3 Calculation_EC 0 00 4 3 Davies 7 2005 2006 11 8 2009 7 9 2 21 27 1991 10 p574 13 2001 11 14 3 44 21 0157 3 Comparison of Three Genotyping Methods for Enterohemorrhagic Escherichia co7 O157 Takeshi SAKAI Sumiko TANABE Misao HASHIDA and Hisako OHMAE 1 21 5
12. 60 15 0kV 28 32 GeneMapper Softwarev 4 0 DNA 4 DigDye Terminator Cycle Sequencing Ready Reaction kit v1 1 v3 1 BigDye XTerminator ABI PRISM 310 Genetic Analyzer 4 IS printing IS printing 5629 32 4 2 PCR 36 DNA 1 0 3 EHEC O157 2 5 PFGE 1
13. A k C 0 005M logf A z2y Debye Hickel Extended Debye Htckel 0 1M Davies 0 5M 3 EC 1 1 1 3 Ri R 2 3 1 33 2 R R 80 Le mS cm Le Calculation_EC 20 y 00099 1 1956 x 0 0775 60 80 Measurement_EC mS cm 50 mS cm to Calculation_EC 10 0 10 20 30 40 50 Measurement_EC mnS cm 11 EC 4 Ci 50 y 0 0036x 0 7964x 0 1211 1 R 09887 Re 1 1 1 1 8 ie 2 NRN lt es 2 MM 1 1 1 O 10 fessme 5 es ia 0 M
14. Extended Debye Hiickel Davies 2 02meq kg 1 15eq kg EC Davies Davies EC EC PC 7 20mS cm 4 1 4 3 3 EC Ag C ECaa EC Extended Debye Huckel Davies EC 4 C EC
15. 2008 2008 7 24 26 7 1988 1994 30 1995 2009 55 2 02meq kg 1 15eq kg EC 0 11 43 2mS cm 1 73 0 054meq kg 1 46meq kg EC 0 0035 0 130mS cm pH EC Na K NH Mg Ca COs HCO F CTL NO SO Fe Fe Mn AT Zn NO PO 2 3
16. Tissue Kit 180 LBuffer ATL Proteinase K 2 2 PCR PCR PCR 1 1 HPLC 3 DNA PCR 100 1zL GeneScan 600 LIZ Size Standard 0 4L Hi Di Formamide 12 95 C 2 5 ABI PRISM 310 Genetic Analyzer 15 0kV 5
17. 3 2 1 H21 V31 5 MLVA 3 6 2 H21 V31 H21 V44 5 IS printing 1 3 1 2 H21 V02 H21 V42 2 H21 V31 H21 V44 5 1 H21 V17 3 IS printing H21 V02 H21 V42 PFGE MLVA 9 6 DNA VNTR DNA VNTR
18. 69 3 60 008 1 3 2 HCO 44 5 61 016 1 3 1 F 55 4 18 998 1 0 1 CH 76 35 35 453 1 0 1 NO 71 46 62 004 1 0 1 NO 71 8 46 005 1 0 1 SO 80 02 96 06 1 3 2 PO 69 0 94 97 1 3 3 3 1 Ri 2 R 7 1 Cation Anion R1 100 x Cation Anion Cation Anion Cation Anion 50 eq L 30 R 30 Cation Anion 50 100 w eq 15 Ri 15 Cation Anion gt 100 z eq L 8 R 8 2 EC EC R 100X EC EC EC EC EC 5zS cm 20 R2 20 EC 5 30zS cm 13 R2 13 EC gt 30zS cm 9 R2 99 5
19. PFGE MLVA IS printing 3 IS printing 1 PFGE 2 MLVA 6 1 3 IS printing 2 PFGE MLVA 18 0157 28 Enterohemorrhagic Escherichia eo7 EHEC O157 HUS EHEC O157 3 EHEC O157 3 5
20. 01001101 1 3 5 7 10 30 el52 100100101000101111 110000110001001101 3 5 7 10 30 el52 100100101000101111 110000110001001101 3 5 7 10 30 el52 100100101000101111 110000110001001101 1 ET 3 7 4 8 37 e204 000100101001101111 110100100011101011 3 7 4 8 36 e204 000100101001101111 110100100011101011 3 7 8 000100101001101111 110100100011101011 1 IO 3 6 7 8 58 e99 100001101000001111 110000110001001101 2 2 01 2 16 sp 2 sr7 1S printing 1 0 1 1 01 1 15 ege 1 16 4 3 VNTR Off set ee 1 2 3 K25 K3 K34 K 9 K17 K 19 K36 K 37 K10 6bp 6 bp 18 bp 6 bp 6 bp 6 bp 7 bp 6 bp 6 bp VNTR TGCCAAA AAGGTG i TCTTTA CCACGA TGCTAC GGCTCT ll0pp 323bp 99bp 466bp 121bp 273bp 102 bp 142bp 178bp K34 VNTR AAATAATCTACAGAAGTT AAATAATTCGCAGGAGTT AAATAATCATCAGAAGTT AAATAATATACAGAAGTT AAATAATATACAGGAGTT MLVA 10 10 000rpm 10 DNeasy Blood
21. 6 6 6 4 28 c293 110100111101111111 010100100111101111 H21 V42 5 15 11 16 7 6 11 11 23 e759 111100111101111111 011100100011101111 H21 V43 4 4 7 0 5 8 6 5 0 e765 000100000000101111 010100001001001101 H21 V44 5 9 10 9 7 6 6 6 41 e492 110100111101111101 011100100111101111 H21 V45 5 15 8 8 4 6 5 4 25 c512 100100100000101111 110100000000101011 H21 V46 2 14 8 16 12 7 4 8 44 e755 011100101001101111 110100100011101011 011100100111101111 011100100011101111 011100100111101111 011100100111101111 011100100111101111 011100100111101111 011100100111101111 011100100111101111 uN Cy 110000100011101011 110000100011101011 110000100011101011 110100100011101011 011100100111101111 011100100111101111 011100100111101111 011100100111101111 011100100111101111 011100100111101111 011100100111101111 011100100111101111 011100100111101111 PFGE IS printing K25 K3 K34 K9 K17 K19 K36 K37 K10 lst Set 2nd Set H21 V01 5 12 9 17 8 6 9 7 21 e64 111100111101111101 011100100011101111 H21 V02 8 12 11 14 6 3 6 26 e65 111100111101111111 011100100011101111 H21 V03 4 20 9 16 9 6 10 6 20 e30 100100110100101111 011000000011101101 H21 V04 4 11 9 13 11 6 10 6 22 e66 110100111101101101 011100100011101111 HIVOS 3 4 7 0 5 8 6 5 0 et 000l00010000101111 010100001001001101 3 5 7 10 W320 e152 100100101000101111 1100001100
22. EC RC CO Me a B TB Extended Debye Htickel A z I logf 0 2 A z I 3 ogf 0 Zi Davies Davies 0 3 0 2 0 2 Extended Debye Hckel Davies EC EE 1 EC A ofi m EC EC i A 0 509 25C B 0 329 25 ri ji i mi I 1 2 mi 2 A 1 NN Br HT 349 81 1 008 3 0 1 Na 50 1 22 99 1 3 1 Kt 73 5 39 098 1 0 1 NE 73 56 18 039 1 0 1 Mg 53 05 24 312 2 6 2 Ca 59 5 40 08 2 0 2 Fe 53 5 55 847 2 0 2 Fe 68 4 55 847 3 0 3 Mn 53 5 54 938 2 0 2 AIT 63 26 982 3 0 3 Zn 528 6537 20 2 OH 198 3 17 007 1 0 1 CO
23. GTTTACATGAGTTTGACGATGGCGATC 0 06 K19 NED GCAGTGATCATTATTAGCACCGCTTTCTGGATGTTC GGGGCAGGGAATAAGGCCACCTGTTAAGC 0 04 K36 6 FAM GGCGTCCTTCATCGGCCTGTCCGTTAAAC GCCGCTGAAAGCCCACACCATGC 0 03 K37 6 FAM GCCGCCCCTTACATTACGCGGACATTC GCAGGAGAACAACAAAACAGACAGTAATCAGAGCAGC 0 04 3 K10 VIC CAGCCTCCTGCAAACTTTACTGTTCATTTCTACAGTCTC GGATCTGTCTGTATCATCATTGAATGAACAACCCATTTC 0 3 2 PFGE PFGE 3 MLVA 1 2 46 EHEC O157 28 5 18 1 DNA HH 2 21 O157 Lb 110100111100111101 110000111101111111 110100111100111101 110100111100111101 110100111100111101 110100111100111101 110100111100111101 110100111100111101 110100111100111101 110100111101111101 110100111101111101 110100111101111101 110100111101111101 110100111101111101 110100111101111101 H21 V34 5 6 8 12 3 4 9 21 34 e574 100100101000101101 110000110001001101 H21 V41 5 9 10 11
24. ME lt SS 44 21 Electric Conductivity of Spring Water Naotaka UEDA and Noriko ICHIKAWA ml Kohlrausch Davies gt 4 EC EC
25. easurement_EC mS cm 1 2 Extended Debye Hickel 1 3 Davies 0 2 R 0 4 03 0 2 01 00 01 02 03 04 3 1 R ECcal AG 0 4 0 2 3 2 R 0 0 0 2 0 4 Extended Debye Htuickel 3 3 R Davies 2 4 R R2 R J Extended 340 XC Dedye Davies uckel 0003 0 08 0075 0039 0000 0096 0 059 0 037 0112 0306 0 169 0 180 0066 0 023 0280 0188 0 026 0065 0 078 0 055 152 152 152 152 ron to 83 3 4 0 0 0 54 26 094 0 82 0 26 0 27 2 54 047 0 26 2 10 R 0 08 0 08 2 R 152 151 R 8
26. ickel Davies 13 3 3 2 Davies 3 EC EC 7 20mS cm EC 20mS cm R 152 4 99 26 9 R 3 99 2z R 5 56 14 9 7 1 0 14 9 4 7 1 6 2 4 56 152 7 2
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