iTC200 Manual（Japanese）
Contents
1.2. ITC ITC 2 ITC
3. OFF TEL 03 5331 9336 ED 9 00 17 30
4. qa b 300 ul 1 16 c 1 2 mm 200 ul lt d 20 pl
5. GE RM 169 0073 A 3 25 ee NN TEL 03 5331 9336 FAX 03 5331 9370 e mail Tech JP ge com
6. Xx 30 4 Wjngow mRawlITC Origin 7 COrigin7U UNTITLED DeltaH File Edit View Granh Data Math ITC Tools Farmat Help a a 3 dL Tile Vertically Arranege Icons Cascade Tile Horizontally Refresh Rename Duplicate Script Window 3 Data1 RAW 4 DatalCoett 5 Results 1 v 6 DeltaH kcal mo of injectant 3 P 42 P 4
7. P 51 1 ucal sec 1 30 Instrument Controls Detergent Sook and Rinse long 20 Contrad 70 Decon 90 50C 30 49 2 PCR 20 Contrad 70 100 pl 3 Instrument Controls Accessory Station Syringe Fill Pipet purge Re Pipet Distance 100 ul 3 4 Accessory Station
8. DP 2 AReg Time Plot DP Final HisgMHHrme Hubration 27 1 Options ITC Equilibration Options TZ Default Usrr Elp bi iled to open DT9817 wstem Cnetficients Start in dwanced Mode IT 1 nh LIHTI IS 2 Fast Egu
9. 8 2 3 0 5 2 ul kcal mol Origin K N AH MicroCal iTCzoo
10. AT 1 MicroCal iTCzoo 2 MicroCal iTCzoo System MicroCal iTCzo 200 pl 300ul 40 ul 60 pl
11. B lt 5 mM TCEP 2 lt 2 mM DTT ITC 11 ITC MicroCal iTC2oo 1 60 300 Kp X50 100 Kp X5 10
12. AH Wiseman Anal Biochem 179 131 1989 ITC hn Mtot C n MLtot KA K D n Mtot KA Kp 1 1 c Kka ckp Cc 1 1000 C 5 250 12 1 1
13. pv PC MicroCal iTCzoo 70 cm NMR 110 240 VAC
14. lt srument Controls Accessory Station syrnge Wosn Lon 9 24 2 3 1 10 mM MES EDTA 5 q b 350 ul 10 mM MES 1 2 mm
15. 2 NJV xX t Mt Mt NDH 1 Xt iamlallala lala gl lrlule sks 6 W ngow Rogw 7C Origin Origin7U UNTITLED DeltaHl File Edit View Graph Data Math ITC Tools Format Help Cascade Tile Harizontally Tile Vertically Arranege Icons Rafresh Rername Duplicate Script Window One Set or sites Two Sets of Sites Sequential Binding Sites Compettive Binding Dissociaion 3 Data1 RAw 4 DatalCoett 5 Results v6DeltaH ee kcal mo of injectant 32 Read Data
16. 1 2 3 ITC pH Good
17. txt Script Window Ee lt re EF Ber Leus dmer T HIT us en ru lh HF hrmas rH TI ma ET a rear ln Fuar 1 Ee an ee mm Me luna BM ee 9 3 6 1 53 1 1 3 2 20 Contrad 70 3 3
18. e 100 ul f g h 7C200 Main Window Instrument Controls Default Boot 30C 17 2 Caz EDTA 2 1 1 2 Options Accessory_Station Advanced User Mode WTTC2HU hefault User ystem ITO mu
19. pln 7 C Wripin7OWNTITLED DeltaHf Ele mm Grach Dta Mah INC Tocks Formet x 2 7 5 1 One Ser of Sres P 41 2 6 riEin O0 UNTITLE File Edit Wiew Graph Data Ma lt N K H 2 100 lter Reduced Chi sqr Chi sqr is not reduced Doe 39 3 N K K AH AS Nr cf at s 5 Pe 4 F
20. 50 pl lt c d P 16 17 4ccessory Station Syringe Fill Cacl q OK Please piace the pipette in the rmst position b
21. Raw Data Raw Data Origin Raw Data AH Data 0 AD Il i kcalmole of injectant 1 a 0 5 1 0 1 5 2 0 Molar Ratio h Tme hin Kp AH AG AS R AHL T A6 RTin Kg HLTAs ao MicroCal iTCzoo
22. kcal mol of injectant 0 0 0 2 0 4 0 6 0 8 1 0 1 2 1 4 1 6 1 8 2 0 2 2 Molar Ratio aq Data 1 Data2 DriEin rigiprU HNTIILED DeltaH File Edit Wiem aph EiE at ITE Tools Farmat Windomw Help ls Im Tisnlay Rangg Peset to Full Rangg Data harkers Mowe Data Fnints m patas_HpH b Concenroto7 0 Inject ol Cell oI For Data Tag ancel In SyringelmiM rrsnrirobni C in CellimiM 0 4 i Inject weol tul 4 Cell Vol tml 0 2054 35 c UI Gem Do Mh
23. KA n 50 100 mM pH 0 05 pH pH
24. 25 30 ITC 25 30 13 50C A C 10 40 C
25. ED Samples lt ED Analysis FEBUF10TTC itcH523cU06 itc RNaHHHTTG Data itc0523cnn1 itc M1mhibitorn175 itc TrainingTTG itc0523c002 itc M1 NolInhibitor itc itc0523c03 itc M2Inhibitorn1 itc iic0523c04Litc M2Nomhibitor itc iic0523cn05 itc PERSSQN7TTG lt 1 NM ICantralTTG T TC Data it Onen as read only 70MNHTITIL ED Deltalf OOA DoAo AN IRC Teoy Pk iAw Ho ERC 2 7 2 2 1 2 E mh WII Fie Edit View Graph Data Mah ITC Tools Format Window Hep a la le a a a dl 0ams_W Available Data Layer Conrents 2 Doto7
26. TEL 03 5331 9315 FAX 03 5331 9349 TEL 03 5331 9336 MicroCoal FAX 03 5331 9370 e mail Tech JP ge com O amp A Science Program Web 6000 Www gelitesciences co jp 2012 GE
27. OK 26 c Cacl OK d OK Make sure the cells are filled Please disconnect the syrinqe tubing and insert the Pipetta into the cell port 5 Start lt DP 9 bcals sec EDTA 1 2 4 Oo7o7s
28. 1 Ca EDTA CaClz EDTA 1 1 3 MicroCal iTCzo Test Kit PC MicroCal iTCzoo iTC2oo with Origin MicroCal iTC2oo 75 Buffer
29. Orig Hi wm Date Ph TC Tocis Fm Wiow He EE 3 3 LL a 3 es 3 EL 10 1 Reove Bad Data 2 H r L L h 11 12 2 11 P 33 2 7 2 2 46 2 7 8 Experimental Design 1 Experimental Design WITC200 Default User System IIC Options Help Accessory Station Failed to open DT9817 Hardware sconnected 2 1 Experimental Moge WTCEEUUH Defanlt User TT High Quality C 50 Aecessory Station Faile
30. mM Injection Parameters EE Volume 2 60 120 sec Spacing Spacmg Spacing P 29 2 5 19 4 Feedback Mode Gain High Feegbgck Mode Gain DP High Low None Sng peed 0 2000 RPM 1 94 Data Fle Hame Tea Feedback Ma Mene olm Dawe Fie Comments 5 Data File Name Training itc
31. OD HH 60 4 Z 2 a O
32. 12 5 ml a Synge Wash Short 1 5 ml 1 5 ml Syringe WashlLongl 2 5 ml 2 5 ml 0 1 Load Ru File Load Run Parameters 2 WATERINJ ITC200 Default User Accessory Station Failed to open DT9817 Hardware disconnected 1 2 Load Run File Instrument Controls Real Time Ph njection Pai
33. MicroCal iTCzoo 2 2 2 2
34. 250 ul 50 Hl c d EDTA 5 2 EDTA q EDTA 350 ul 1 25 b 1 2 mm
35. 10 uM Kp 0 1 uM C 10X10 6 10 7 100 Kp 100 uM C 10X10 6 10 4 0 1 AH IKCaUmola of Injectart lalar Ratio gt 1 1 10 20 MicroCal iTCzoo 2 ul 18 1 1 0 4ul 1
36. 3 30C P 17 Al B C D E C1 C2 C3 C4 C5 3 21 2 Instrument Controls Accessory Station Ce gng Synge Wash 3 qa b OK Buffer 3 ml Yow mmy remove he wash tool and fi the celis ft desired c
37. ki 27 2 5 kk 29 2 6 kk 29 2 7 ki 30 2 7 1 kk 30 2 7 2 2 33 2 7 3 kk 36 2 7 4 39 2 7 5 LL 39 2 7 6 41 2 7 7 42 2 7 8 Experimental Design LL uuu uu uuriartantastastestesteeseesteseereenea 47 MR 49 EO FN TR i OC 49 kt 49 LL 49 TONDO I TF NN 49 kk 49 50 YY A TW dctAcccAAl GG 51 Y Axis 52 LL 54 Introduction MicroCal iTCzoo
38. pH pH 0 05 HCI NaOH 0 05 pH pH 10 DMSO 100 DMSO 10 100 mM DMSO DMSO DMSO DMSO 1 2 5
39. 3 4 Instrument Controls Accessory Station Ce Water Rnse Long 5 srrument Controls Accessory Station Syringe Wash 6 Main Window System Quit Progrom 7 ITCzoo Off 8 PC 9 UPS Off On On 2 Off 54 mm owae_hecar ra RG E 1 1yNsoorep L 22GA Blunt T ee Syringe
40. 46 103M C Cc 1 1 10 uM AH n Kp Displqcement ITC 46 10 C Kp X10 50 1 2 mM Kp XX 1 5 1 100 10 uM C 1
41. CqClz 29 2 7 EDTA CaCb ITC 2 7 1 1 Origin iTCzso reea ULC TC aD 2 Read Data TOWUNTITLED miamITt Ee untm Rh ITH Toste Form mmmm Hee a lalla mm visrairai il ra cade ac cE me mr He lnITC ne0EG3c0OEme DT Ll nlsvairall ia H FLT PU rare Ir 9 mi lh ar i tb 3
42. 50 100 AH Kp n 1 1 Kp 100 uM 10 uM C 0 1 4 1 mM EE i 14 MicroCal iTCzoo Experimental Desjgn P 47 48 15 MicroCal iTCz o MicroCal iTCzoo Test Kit KIT 0209 88
43. ndh data1_ndh Layer Contents 33 Rescale on OK Layer 1 wallahlg Data LareT Contents OK wa HataZ ridh arp Layuer Frnnerlies Flat A amp ssociations data cnelf spline Edit Hange datalcoelf_ coeff 5hnwi Hanne ly Hescals nn HE Show curreht folder only Rescale on OK Graph Rescale to Show All riEIm riEinI NTTTLED DeltaH File Edt ism Ei ata hath I Tools Format Windnm dd Plot to Lawgr k ee dd Errnr Bars ail la gl IA 34 0
44. MV_ PDF TIF Final Figure pcakbsec KCalMole of Injectant 40 2 7 6 Origin 5 1 One Set of Sites 1 2 Two Set of Sites 2 3 Sequential Binding 2 4 Competitive Binding Displacement ITC 5 Dissociation 41 2 7 7
45. OK 3 g OK Plaasw move the Pipette Syringe nssembhy to the clean poson 4 CaClz 100 ul 4 4
46. Setup lastrun1 ini NoiseTest inj testin Total Injec Cell Tempera Heference P Initial D elay Syringe Cont WATERI Edit Mc Cell Concent gt Unique Stirring spee Ni WaTERINJ T Setup Files enj gt 1 High 20 Data File Na None 3 Experimental Parameters Total Injections Cell Temperature Initial Delay 60 Stirring Speed 1000 Injection Parameters Filter Period 51 Y Axis y Axis DP Deferential Power 3 1 1 2 3 I7C gt Start ITC Calibration Run gt
47. 1 Origin iTCzso 2 Read Data 3 4 s B 4 54 ww NI 3 Window mRawlITC Tile Harizontally Tile Vertically hrrange Icons Refresh Hemoye Bad Data Rename pplese Subtract Reference Data Script Window 3 Datal RAW 4 DatalCoett 9 5 Results v 5 DeltaH kcal mof of injectant 42 4 Adjust Integrations UNTITLFD mRawTC 33 oe 3 53 7 606 13 3 647 00 1333 3 30 6 Tirme min 2 _ Baseline PH Wrii TITLED mmwlTC Pa Fe ER ou Goch Daa Mah IfC tooch Formk Wndew He 43 6 Sn of Points
48. OK Pimasm place the pipette in the rast position dm p Cc 22 d 6 f OK CC IOWIY IO Loosen fmtaanin nut mt boattom of Prpette Synnge assembly OK Aiiach the Fill Por Admptor to the Ppette Syninge assembly Vamimd Do no overtghten ms hs may break he syrmge
49. 3 Aufo Mode 4 Orjg Sofwore DP 7C200 Real Time Plot Axjs Loyer 7 From 0 7o 1200 DP Scale Controls Edit ronges Axis Rescale Ranges Options Full Scale Auto View 1 0 15 OK 28 Display Mode Rescale to Show All DP Scale Controls Saved View 2 Auto View 1 Auto View 2 Saved View 1 Edit ranges A Axis Rescale RanqesJOptions Cancel Full Scale Auto View 1 Full Scale Auto V
50. System ITC Opticns Heb Accessory Station Falled to open DT317 Hardw Zz load Run File_ Save Run Fike mn 5 Adyanced Experimental Design Int Expenmental Pasameters Total Inections 16 Cell Temperatme C 30 Reerence Power ECal sec 0 12 25 4 15 Initial Delay sec _60 Syringe Concentration mM 0 0 Cell Concentration mM 00 Naone Low ig Setup Data File Path C ITC200 data Dgorg File Path rh Adyanced Experimental Design Real Time Plot 5ewp Setup File Path ICE MTC200tsetup Data Path I c Systeml gt 3ITC200 Init Setup File Lastrun1 ini Cells Boot Temp 30 FE Eurrent User Default User FolderName OK Cancel 20 6 1 1 Edit Mode Unjgue Injection Parameters Vo ume 0 4 Duration 0 8 2 2 1
51. Per 8 Baseline TihWlulrllllP lm PP Fa We pmh Pkn PAMh ITC Tnnh PFmmw Wnn WE aa Fecha jk tn eid dn wi pt Lat fr let ho lrcm em ha how heladt fr ho dm 1 TeaiHAm ep tN gt gt 5 CF ON TTL ED emm Pa J Fe Ed Vem Gronkh Pa Math ITC To Fmmw Wi WEE 44 7 lt lt 2 250 8 Quit regrgreg all peaks ripin 7 CW NTITLFD mRawiTC ELSE 45 9 AH
52. Syringe Wos Long Accesso y Station Dry Synge NR Contrad 70 Decon Laboratories Instrument Controls Accesso y Station Cell and Syringe Wash Ce gu er Rnse S or7 Accessory Station 3 5 ml syinge Fi Cell Water Rjnse Long 2096 Contrad 70
53. 1 5 500 1000 C 1 1000 5 Advanced Experimental Desjgn 48 PP MicroCal iTCzoo Ce gng Syringe Wash P 21 2 2 2 3 1
54. 500 uL 3 22GA Blunt Tip 1ISYN50018P 1ooolmesotts50 Tube Polypropylene PCR with Cap 200 ul 1000 ITTB501150 ptsootoos TIP PLUNGER 100 wl Pack of 5 5 TIP130010 005 H9 3 NN OU 6 DR 9 F
55. 6 al 4 a IF ml lt 1 5 W gow Data1 Oriein 7 1C 0Oriein70 UNTITLED Datal i File Edit View Plot Column Math Statistics Tools Format a Cascade a Tile Horizontally EE Tile Wertically hrrange Icons Retresh Rename Duplicate Script Window Falders 31 1 DUH ucqls 1 2 2 ucals sec
56. G 2 Data1 3 Datal1 Raw kcal mo of injectant 8 Data2RiW 9 Data3Caeff 1 Layer Contents data2_ndh EH OK Layer 1 Lawer Properties Flot amp ssociations 1 TnLI Edit Hanae ala1cnelff_cnelf Show Range Sort Rescale nn OK Shaw current folder only Subtract Straight Line Y Translate Linear Regressinn Concentration Hemoye Bad Data Select Fitting Function Start Fitting Sessinn C operator 3 v2 OK Y DATA1_NDH A gt Yl DATAT_NDH Help YsY1 Ye Y Y1 dala set YZ dala gt Yz sm az657 DalatBEGIN 38 2 7 4 1 Aemove Bad Data
57. GE Heqlthcare Life Sciences MicroCql iTCzoo Sustem GE Imagination at work LE A ol fl cc 6 i 6 6 7 ITC kk 7 8 es 9 kL 10 10 GG 10 DMSO 11 i AcAlAl Gl GcccciiiSc 11 eee ee 11 ITC esses 12 es 12 es 12 cc Ep hkhbhkhbhbhbhbhkht 12 i 13 13 kk 13 13 Kp 10 8M LL 14 Kp 10 Li 14 ee 15 OR 16 ck 16 1 i 16 2 Caz EDTA kk 18 2 1 kk 18 2 2 21 2 3 kl 25 2 4
58. Lnad Demo File Gywstem Coetficients Start Im dvanced hode ITE Equilibration Options Cessory otation iled to open DT9817 Hardware yy Lnad Run Fil Ernerimental Desi Retorence Power Ca sec OU 12 25 In Detay me Syomge Concentiatson mM Ce Cancerdsatmn lm Simmm Speed 0 2000 RPMU Data Fie Nawe Tsang tc Teedbeck Mode ain ol Mene High Dwe Fie Comments 1 1 0 4 pl 1 2 0 150 18 1 Edit Mode All Same 18 Smm TC Otors Thermonating IIC 25 ser Te gt 2 mi awi hm Pe NM Pun Pe Sat EF upeeimaerd al Deep Advanced Epewwemtol Dosign Insinumaed Cordrele Experimental Parameters Total Injections Cell Temperature 2 80C a 1o ii De ogy 0 Syrjnge Concentration
59. Lnad Run ile Saw Pun File Adyanced E pel Minimun_Protein C 10 Experimental Hesinn Ernerlmertal Hode Mnmun Pen High Speed 1 SIM 3 2 Starting Value N Kd A H N System ITC Options Help Accesson Stater Kd La gz a Help maimene ete 1 uM 1e 6 AH NO 5 10 nw mm kcolmole EE 5 a dyanced Ex Experim
60. NC Tocb formw mm Ke 2 7 3 2 2 1 2 1 q Subtract Re7erence Doto n Origin 7U UNITIIL Une Set of Sites Two Sets of Sites Dissociation b Data Reference OK Subtract Reference Data lle x Data Reference Jatal ND Reference IData2_NDH _ Cancel 36 2 a b Window Data2 a Or mr Or gmIOYUNTITLEE 1 Fike Edd Vw Plot Coknn Math Statcs Tooh Formt ME He la
61. O CN hm Pe
62. Yaxis WTTCG2UHU Default User System ntinmns Halp Frint awe fs Text Run P ters Ro ltn nnen DTSH17 Hardware disconnected Sim hode IE nn Instrument Controls dvranced Experimental Design Expermental Design 4 ITCY Axis Calibration Data File Name TTG TY xis Dalihratinn Filg DP Calibration ParameIErs Experimental Temp 3n Data File Hame default itk Number of Pulses IE Reference Power Filter Period 5 Ftirrindg 5 ne 1000 gt FeeHhark nde aim nne Lnw Hinh Pulse Duration lser Pulse Spacing Er Start Hun Cancel 5 Start Run 6 DP DP Final Baseline Equilibration 7 8 Script Window Script Window 1
63. ental Desiqn arting Values Please enter N yalue and press Enter Estimated alues N Help kd 0 M _Help AH kcaks ale Help 47 4 Update Experimental Curve Resurts 1 Experimental Mode Highest Huality Help Total Heat uCal Help 2 Starting Values Cell ukd Change 50 Help Please enter dH value and press Enter Syringe phd Change 500 Help C Value Ch eb Estimated Values N 1 Help Kd nnnnnn1 MM Help AH 5 kcas ole Help delta H kCalmnle Leqend ilhin Optimal Hanae Temperalure 25 Degrees C Data File Name Dutside Optimal Range default itc Dutside Suggested Fiange Update Experimental urye Dptions Use Ka r Flat Results Change C egeng C 5 500 C
64. iew Saved View 1 Y Min F10 Saved View 1 Y Max 10 Saved View 2 Y Min 50 Saved View 2 Y Max 50 X Axis Options Rescale DP Scale Controls Auto View 1 2 5 1 Injection Parameters 2 Edit Moge App To Rest 3 Duration sec _ Volume pl Injection Parameters Edit Mode RE TON a Al Same Unique Apply To Rest Volume Duation Spacing Fier 1 0 4 0 8 150 5 2 3 2 0 4 0 150 5 20 40 TE0O 1 5 5 2 0 4 0 150 5 5 Fi 2 0 4 0 150 5 4 Update Run Parameter yd Lna Run Filg 2 6 Sawe Ruri Filg a Lisplay Run Faram Npdate Run Faram EDTA MES CaCl 0 4 mM
65. ile Save project gs OriEin 7 C Oriein7U UNTITLED DeltaHl il Edit View Graph Data Math ITC Tools Farmat Window Help He CrN Open a Open Excel CtrltE Lndate to Origin 7 0 jnterface pata1_NpH Append Data1_F xz Close 5 Da Dam1_NDH Model 0 resik CHADoF Z15Z Set Detault Folder nm14nles kK Sa1E 502 MT 1 Save Prc sH 1 5BG5E 25ja1 ma s lt 1 8camcliden Save Window ns TT Save Template As 5 TC Final Figure riEim J rigin 7 HNTITLED IT FINAL File Edit Wiem Tanmh hata ah me Tools Fnrmat Windanw w Ligand is in Swringgl Ligand is in Gelh Time nin we Displaw Calories Hisnlay Watts laules Egr Copy Page Fe Export Pogge
66. le la ol saa Sa ee I AI MO ve lk 1 N 3 l 0 MW 1 NDH Statistics Descriptive Statistics Statistics on Co umns nmeiaiaia Es ae g 3 zu C 3 Mean y ripin OWNTITLED Da Coamn Hoth antsdics Tooh Format Wirriow He aman A ma a 3 Elum EI 37 c Window DeltaH Origin COrigin7U UNTITLED DeltaH File Edit View Graph Data Math ITC Toos Farmat Help eee 2 x a Tile Horizontally Tile Wertically Arrange Icons Refresh Hemoye Bad Data Rename Duplicate Subtract Reference Data Script Window Al 3 Folders 2 1 mRawlT
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