MightyAmp® for Card
Contents
1. FTA PCR PCR v201007Da Woo o ci a BEERS anen TaKaRa R DR2. MightyAmp DNA Polymerase 1 25 U ul 200 ul 2 X MightyAmp Buffer for Card Mg dNTP plus 1mlx5 MTE 2 xX 4mM dNTP 2X 800 uMeach 50mM Tris HCI pH8 2 at 4 100 mM NaCl 0 1 mM EDTA 1 mM DTT 0 1 Tween 20 0 1 Nonidet P 40 50 Glycerol DNA 74 30 10 nmol 1U 1 10U 0 6 ug DA Hind il 74C 1 DNA 2 10U 0 6 ug supercoiled pBR322 DNA 74 C 1 DNA One Polymerase Chain Reaction PCR DNA PCR PCR MightyAmp DNA Polymerase PCR 3 A 1 PCR T Vector
3. MightyAmp for Card Code No R072A Shipping at 20 C Size 200 reactions Stored at 20 C Components MightyAmp DNA Polymerase 2 X MightyAmp Buffer for Card Mg2 dNTP plus Lot No Conc 1 25 units ul Volume 200 ul Expiry Date Regarding general reaction mixture and PCR coditions please refer to the product manual supplied with MightyAmp for Card Storage Buffer 50 mM Tris HCl pH8 2 at 4 C 100mM NaCl 0 1mM EDTA 1mM DTT 0 1 Tween 20 0 1 Nonidet P 40 50 Glycerol Unit definition One unit is the amount of the enzyme that will incorpo rate 10 nmol of dNTP into acid insoluble products in 30 minutes at 74 C with activated salmon sperm DNA as the template primer Purity Nicking endonuclease and exonuclease activity were not de tected after incubation of 0 6 u g of supercoiled pBR322 DNA 0 6 ug of A Hind III digest with 10 units of this enzyme for 1 hour at 74 C Application DNA amplification by Polymerase Chain Reaction PCR This enzyme is recommended for Direct PCR from blood buccal cell and plant tissue etc preserved on Whatman FTA Card without DNA extraction or purification steps PCRamplifcation with blood samples preserved on filter paper PCR products As most PCR products amplified with MightyAmp DNA Polymerase have one A added at 3 terminus the obtained PCR product can be directly used for cloning into T vector Also it is possible to clone the pr
4. oduct in blunt end vectors after blunting and phosphorylation of the end Supplied 2 Xx MightyAmp Buffer for Card Mg2 dNTP plus Size 1ml vial x 5 Mg2 concentration 2 X 4mM dNTP concentration 2 X 800 u M each Storage 20 C NOTICE TO PURCHASER LIMITED LICENSE L15 Hot Start PCR Licensed under U S Patent No 5 338 671 and 5 587 287 and corresponding patents in other countries Note This product is intended to be used for research purpose only They are not to be used for drug or diagnostic purposes nor are they intended for human use They shall not to be used products as food cosmetics or utensils etc Takara products may not be resold or transfered modified for resale or transfer or used to manufacture commercial products without written approval from TAKARA BIO INC If you require licenses for other use please call at 81 77 543 7247 or contact from our website at www takara bio com Produced by TAKARA BIOTECHNOLOGY DALIAN CO LTD v201007Da MightyAmp for Card Code No RO72A Size 200 Shipping at 20 C Stored at 20 C Lot No
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