PrimeSTAR GXL DNA Polymerase
Contents
1. v2007 08Da Bemeoecapes TaKaRa Tel 077 543 6116 Fax 077 543 19772. PCR PCR 1 DNA PCR 20 24 27 30 kb 2 DNA PCR 0 5 1 2 3 4 6 kb 10
3. GXL DNA Polymerase 1 25 units u 50 units 5 X PrimeSTAR GXL Buffer Mg2 plus 400 u dNTP Mixture 2 5 mM each 180 u I O5 X PrimeSTAR GXL Buffer Mg plus lt Mg 5 x 5mM 20 C dNTP Mixture dATP dCTP dGTP dTTP PCR 2 5mM pH pH7 9 98 20 C 50mM Tris HCl pH8 2 at 4 100mM NaCl 0 1mM EDTA 1mM DTT 0 1 Tween 20 0 1 NonidetP 40 50 Glycerol DNA 74C 30 10 nmol 1U OnE 1 10U 06 u g DA Hind ill 74 C 1 DNA 2 10U 0 6 u g supercoiled pBR322 DNA 74 1 DNA OR Polymerase Chain Reaction PCR DNA PCR PrimeSTAR GXL DNA Polymerase
4. 0 u l vial Concentration 2 5 mM of each dNTP pH pH7 9 Form Dissolived in water sodium salts Purity 2 98 for each dNTP Storage 20 C Regarding general reaction mixture and PCR coditions please refer to the product manual supplied with PrimeSTAR GXL DNA Polymerase Note This product is intended to be used for research purpose only They are not to be used for drug or diagnostic purposes nor are they intended for human use They shall not to be used products as food cosmetics or utensils etc Takara products may not be resold or transfered modified for resale or transfer or used to manufacture commercial products without written approval from TAKARA BIO INC If you require licenses for other use please call at 81 77 543 7247 or contact from our website at www takara bio com NOTICE TO PURCHASER LIMITED LICENSE P1 PCR Notice Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US 5 079 352 5 789 224 5 618 711 6 127 155 and claims outside the US corresponding to US Patent No 4 889 818 The purchase of this product includes a limited non transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser s own internal research No right under any other patent claim such as the patented 5 Nuclease Process claims in US Patents Nos 5 210 015 and 5 487 972 no right to perf
5. 4 C 100mM NaCl 0 1 mM EDTA 1mM DIT 0 1 Tween 20 _ 0 1 NonidetP 40 50 Glycerol Unit definition One unit is the amount of the enzyme that will incorporate 10 nmol of dNTP into acid insoluble products in 30 minutes at 74 C with activated salmon sperm DNA as the template primer Purity Nicking endonuclease and exonuclease activity were not detected after incubation of 0 6 u g of supercoiled pBR322 DNA 0 6 u gof A Hind lll digest with 10 units of this enzyme for 1 hour at 74 C Application DNA amplification by Polymerase Chain Reaction PCR PCR products A significant percentage of PCR product obtained using PrimeSTAR GXL DNA polymerase will possess blunt ends Thus obtained PCR products can be directly cloned into blunt end vectors If necessary phosphorylate PCR products before cloning PCR test Good enzyme performance was confirmed by robust single fragment DNA amplified by the standard protocol using human genomic DNA as a template amplified fragments 20 24 27 30 kb Good enzyme performance was confirmed by robust single fragment DNA amplified by the high speed protocol extension time 10 sec using human genomic DNA as a template amplified fragments 0 5 1 2 3 4 6 kb Supplied 5 X PrimeSTAR GXL Buffer Mg plus Size 400 u l vial Mg concentration 5X 5 mM Storage 20 C Supplied dNTP Mixture Mixture of dNTP ready for use in PCR reactions without dilution Size 18
6. High Fidelity PCR High Fidelity PCR 30 kb GC High Fidelity PCR cDNA DNA Polymerase 3 5 exonuclease PCR 1 min kb PCR 2 10 sec kb PCR eas PrimeSTAR
7. PrimeSTAR GXL DNA Polymerase Code No R050Q Size 50 units Shipping at 20 C Stored at 20 C Components PrimeSTAR GXL DNA polymerase 5 X PrimeSTAR GXL Buffer Mg plus dNTP Mixture Lot No Conc 1 25 units p Volume 40yu 1 Expiry Date Description PrimeSTAR GXL DNA Polymerase provides superior extension in high fidelity PCR due to the improvement of excellent high fidelity PCR enzyme PrimeSTAR HS DNA Polymerase and the adoption of original extension factors PrimeSTAR GXL is able to amplify the longer targets than 30 kb successfully with high fidelity This enzyme also makes it possible to amplify the GC rich targets without special buffer or special conditions n addition PrimeSTAR GXL shows good performance even under the condition that a large quantity of nucleic acid is included in reaction solution although general high fidelity PCR enzymes cannot work in such a case Therefore PrimeSTAR GXLis extremely useful to detect the ow expression genes using high concentration of cDNA as a template Furthermore an antibody mediated hot start formulation prevents false initiation events during the reaction assembly caused by mispriming and primer digestion nthe standard protocol the extension time of 1 min kb is recommended The high speed PCR extension time 10 sec kb using twice volume of enzyme is also available for various targets Storage Buffer 50mM Tris HCl pH8 2 at
8. orm any patented method and no right to perform commercial services of any kind including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration is conveyed expressly by implication or by estoppel This product is for research use only Diagnostic uses under Roche patents require a separate license from Roche Further information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA M54 PrimeSTAR HS DNA Polymerase This product is the subject of the pending U S patent application and its foreign counterparts L15 Hot Start PCR Licensed under U S Patent No 5 338 671 and 5 587 287 and corresponding patents in other countries Produced by TAKARA BIOTECHNOLOGY DALIAN CO LTD V2007 08Da PrimeSTAR GXL DNA Polymerase Code No R050Q Size 50 units Shipping at 20 C Stored at 20 C Lot No PrimeSTAR GXL DNA Polymerase I4 PrimeSTAR HS DNA Polymerase PCR
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