A-12-78 インベーダーUGT1A1アッセイ_4.indd
Contents
1. DNA G7747 6A 1 6 2 6A 6 Flap Flap 3 6 2 Flap 2 Flap 6FRET FAM FIlap 6FRET FAM Fluorescein2. DNA 20 70ngL DNA UGT1A1 UDP UGT Uridine diphosphate glucuronosyltransferase 1 0 C7747 25 0C7747 6 0 C7747 UGT1A1 3 7 0C7747 0C7747 25 0C7747 6 UGT Ando 118
3. Ando 1 9 _0G7747 19 93 5 6 11 9 45 ppV 45 28 6 10 29 39 33 26 INp 80 11 57 68 58 16 3 41 26 92 118 100 22 95 095 123 4 3 28 6 3 25 6 6 25 6 28 6 28 6 25 Minami 117 0 7747 2
4. 63C 4 63 1C 15 25C 560nm 620nm R Signal 485nm 530nm F Signal 1 R Signal FOZ Fold Over Zero F Signal FOZ Ratio R Signal FOZ R Sienal R Signal F Signal FOZ F Signal F Signal Ratio R S
5. 28 6 6 28 28 6 7 28 6 7 28 7 7 28 7 7 28 6 6 6 G G 6 G G 6 6 G A 6 G A 6 A A A A 1 3 4 0 005amol zL DNA 5 1 07747 29 SEP i 49 28 T 6 7 7 7 0 28 6 6 130 0 0 130 28 6 77 0 38 0 38 28 7 7 0 0 5 5 130 38 5 173 2 0C7747 6 PCR RELP 0C7747 6 PCR RELP 6G G 6G A 6A A 6 G G 1l5 0 0 115 6 G A 0 53 0 53 6 A A 0 0 2 2 115 53 2
6. 5 6 28 28 28 1 28 2 28 FRET FAM 28 FRET RED 28 1 28 2 6 6 6 1 6 2 6 FRET FAM 6 FRET RED 6 1 6 2 DNA UDP C7747 25 0C7747 6 UDP
7. Redmond Red Fluorescein 0C7747 6G G G A A A 1 1 EDTA DNA 2 DNA DNA DNA DNA Qiagen QIAamp DNA Blood Mini Kit PN 51104 3 DNA DNA Molecular Probes PicoGreen dsDNA Quantitation Kit P 7589 DNA DNA 20 70ng L 4 DNA 20C 30 4C 21 30C 7 2 2 DNA Qiagen AW2 16 1
8. 5 DNA 5 FIap 5 Flap 3 6 1 Flap 6 1 6 2 DNA 6 2 FIap 2 Flap 6FRET RED FIap 6FRET RED Redmond Red 6FRET RED 5 Redmond Red Quencher 3 Flap
9. fsobe 0C7747 40 96 4 1 5 14 80 ppv 80 28 29 6 e pb IB NPV 81 16 7 x 10 26 36 100 28 95 156 177 Du 3 28 6 3 28 6 6 28 6 28 6 28 6 28 1 3 R Signal FOZ F signal FOZ AO 28 6 6 R Signal FOZ 3 25 8 28 6 7
10. 1 20 L 1 95C 5 63C 28 6 107L 1
11. Cat MLL 9601 Cat M5904 Corning Cat 4871 13 3 x 8 3cm DS No 35PS Cat 7640105 DNase RNase 1 5mL 2 0mL 8 10 L Microsoft Windows Microsoft Excel UGT1A1 DNA Mini Kit PN 51104 DNA Molecular Probes PicoGreen dsDNA Quantitation Kit P 7589 Qiagen QIAamp DNA Blood 3 1 2 3 4 5 6 7 8 9 10 11 12 13 10 1 DNA 10 L
12. 3 1 2 DNA DNA Molecular Probes PicoGreen dsDNA Quantitation Kit P 7589 DNA 20 70ng DNA 70ng QIAamp DNA Blood Mini Kit Buffer AE DNA 20 70ng DNA 20ng Millipore Microcon YM 100 100kDa DNA 20 70ng 1 1 15 25C SISIS 28 4 1 28
13. 6 3 1 01 1 121C 20 2 0 1 3 4 4 1 20C 2 27 50 28 0 65mL 1
14. 6 25 28 6 28 6 25 55 UGT1A1 3 Minami 8 6 28 PPV 80 NPV 80 16 0 95 1 61 159 80 6 25 6 25 80 36 07747
15. 3 Tsobe 6 25 PPV 80 NPV 81 167 95 1 56 177 80 6 25 6 25 81 0C7747 Minami 9 1 CC7747 rv 29 98 4 1 5 9 80 ppv 80 5 9 i 0 0 2 NMM 80 160 nu 14 41 55 100 25 95 161 159 0C77 4 7
16. R SignalFOZ gt 2 20 28 6 7 F SignalFOZ 2 20 28 7 7 F Signal FOZ 3 25 6 G G R Signal FOZ 3 25 6 6 G A R Signal FOZ 2 20 6 G A F Signal FOZ 2 20 6 A A F Signal FOZ 3 25 2 a i 28 6 6 28 6 6 28 28 6 7 28 6 7 28 7 7 28 7 7 6 G G 6 G G 6 6 G A 6 G A 6 A A 6 A A 3 3
17. 0C7747 4 3 6 25 6 25 6 25 PPV 45 NPV 80 3 41 95 0 95 12 3 45 6 28 6 25 80 28 6 6 6 7 7 7 25 25 25 6 G G G A A A 6 6 6 0C7747
18. 1 DNA UDP UGTIAlI 28 UGTI1A1I 6 Flap DNA Flap DNA 0G7747 6 0C7747 29 0G7747 6 0G7747 211 G A DNA G7747 6G 1 0G7747 6 6 1 6 Filap DNA 3 1 3 3
19. 28 1 28 2 6 6 4 1 6 28 6 1 6 2 2 3 20C 12 2 CES BO BO Gy TECAN Infinite F200
20. 6 28 PPV 92 NPV 58 15 3 95 1 90 122 92 6 29 6 25 58 7747 Minami 9 0C7747 20 98 11 1 12 10 92 ppV 92 29 6 21 28 49 42 43 NPV h 58 23 33 56 48 41 15 3 55 62 117 100 47 95 190 122 3 25 6 3 25 6
21. 28 1 02 mLx1 28 2 0 2 mLx1 6 0 65 mLx1 UGT1A1 6 1 02 mLx1 6 2 0 2 mLx1 0 35 mL x1 04 mLx1 1 26 10 2005 2 60 1870 2005 3 16 339 2004 4 Hasegawa Y Sarashina T Clin Chem 50 1479 2004 5 60 2191 2005 6 Ando Y Saka H Cancer Res 60 6921 2000 7 Sai K et al Clin Pharmacol Ther 75 501 2004 8 Minami H et al Pharmacogenetics and Genomics 17 497 2007 9 Isobe H et al J Clin Oncol 25 18S 2539 2007 10 BIO Clinica 23 7 75 2008 11 30 79 2009 12 0120 249 977 FAX 0120 247 477 13 5
22. 170 6 DNA 1 1 HIV HBV HCV 2 28 6 300 2 1 20C 2 3 4 5
23. 200202 004 22000AMX01640000 2013 2 4 2011 1 3 UDP UGT1A1 83021000 UGT1A1 1 2 3 4
24. ignal FOZ 1 F Signal FOZ 1 0 04 0 04 Ratio 28 R Signal FOZ F Signal FOZ Ratio 1 75 5 0 25 6 6 gt 2 0 5 0 1 75 175 0 5 2 0 28 6 7 gt 0 2 0 5 1 75 0 2 25 7 7 R Signal FOZ F Signal FOZ Ratio 1 75 5 0 6 G G gt 2 0 50 1 75 S175 0 5 2 0 6 G A gt 0 2 0 5 1 75 lt 0 2 6 A A Ratio Signal FOZ 2 1 25 6 6 6 G G 2 25 7 7 6 A A
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