アプティマTM Combo 2 クラミジア／ゴノレア
Contents
1. 1 1 1 df 50 000 RLU 2 rRNA 5f g 100 000 RLU 3 000 000 RLC 3 2 5 0 f g2. 2 3 0 6 0 7 0 2 0 9 0 2 APTIMA STD 1 2 3 4 4 1 1 TO 9 SS 1 2 0 3 0mL
3. 4 1 3 2 5 1 2 5 396 Pseudamonas putida Rahnella aguatilis Khodospirillum rubrum Saccharonmyces cerevisiae Salmonella minnesota Salmonella typhimurium Serratia marcescens Staphylococcus saprophyticus
4. 1 5 CT GC Kinetic Type 6 Kinetic Type RLU 1 0 0 0 4 5 0 0 0 0 0 Kinetic Type gg trachomatis Me7ssez7g RE gonorrhoeae STD Sexually transmitted diseases
5. 2009 11 4 2009 4 3 ABEO04T 21700AMY00210000 Combo 2 C TIGRIS 1000 1 2 G TIGRIS 250 Ng 1 5 30 3 4 NN 4 6 mbo 2
6. m 1 0 3 3 1 2 1 0 0 0 p pm i 2
7. NO SM 3 Ve 3 APT IMA STD 2 3 0 6 0 7 0 2 0 90
8. i 20uL 200kL 1 000z 1 250zL 5 1 25mL 5mL 12 5mL 1 TTC 5 2 1 4 5 A a DO 76 20 1 5 Ma 3
9. 0 1 1 Combo 2 C TIGRIS 1000 OOo _ W Combo 2 TIGRIS 250 A TIGRIS TCR PR S Combo 2 TIGRIS 250 B TIGRIS B TCR B A E P CT PCT NGOC GC PGC NCT 2 18 Combo 2 C TIGRTIS 1000 1 1000 Combo 2 TIGRTIS 250
10. 2 4 cm 2 3 2
11. 7 Achramobacter xerosis Acinetobacter calcoaceticus Acinetobacter Iwoffi Actinamyces israelii Actinamyces naeslundii Actinamyces pyogenes Actinamyces viscosus Aerococcus viridans Aeromonas hydrophila 8 Agrobacterium radiobacter Alcaligenes faecalis Bacillus subtilis Bacteroides fragilis Bacteroides ureolyticus Bifidobacteriun adolescentis 2 Bifidobacterium brevi Branhamella catarrhalis Brevibacterium linens 1 Campylobacter jejunt Candida albicans Candida glabrata MTU 1 1 C 2
12. 7 E Target Cap HC System TCS 8 DTS
13. 5 2 1 2 TU 4 1 3 42Cx1 62 Cx2 2 VWR Multi Tub TU MTU NN NG
14. RNA DNA 30 KC O HLO 0 765g L a KC1 31g L HPA Hybridization NaCl 51g L MgCl 51g L Protection Assay 3 KH PO 20g L CaCl 1 6g L MnSO 14pug L Na S 0 5H O 5g LL
15. 2 NG TS Ne 2 DKA Dual Kinetic Assay SN 20mg L 15mg L YAMA 1 2mg L 330g L SA D 40mg L 70zg L CN a 1g L B 270ng L G A 0 4g L 0 39g L 0 12g L A 1 0 C i AB DNA 3 HP A 2 rRNA
16. RT Kinetic Type 3 RE Combo 2 85 000 ERLU lt r R NA 250 000 RNA R00 00 A HI 4 5
17. TU TCS 2 2 3 2 U 7 5 TU 2 0 0 TU U U 6 2 1 1 0 U 4 2 1 U 2 5 TU 4 2 1
18. gp eS mw 7 6 bi da ts 15 0 000 Pe en 4 A 3 rr EE NG Kinetic Type 2 1 9 9 6 0 1 9 5 BR 1 2 4 85 000 lt RLU lt 0 I MT
19. 2 es A 4 HC DTS APTIMA STD2Z APTIMA STD 5 6 5 7 DTS APTIMA LE EE 6 a 1
20. 6 7 3 2 5 3 0 4
21. 1 Target io lt Sequence oi 6 Capture Bead 8 8 Sequence Oligo 8 6051 a 8 Capture 8 iE Oligo Tail iG AAAAAAAAAAAAAAAAA _ ss 8i8 1 TrmTrrmrT 8 8 GB vwvvvvvvyvyvyvyvvyvy 8i8 Capture 3 R TT 3 i 8g Oligo Tail g gi E 8i8 Capture 83 Target 3 Sequence Sequence 1 1 2 TMA Transcription Mediated Amplificstion rR NA MIMLV Moloney Murine Leukemia Virus RNA DNA RNA MMLV R NA DNA NERO IS DNA R NA RNA RNase MMLV rR NA
22. TTU Capture System 3 Target U RNA 1 0 0 1 TU CT 2 GC 4 0 0 TTU 40 0 3 U U 6 2 1 3 0 6 0 2 TTU 1 5 30
23. i 5 6 2 5 3 1 1 5 3 0 2 B RNA RNA 1 5 3 0 3 0 3 3 1 1 gt 5 2 8 3 0 157 5 ME TE 1 1 A i i 5 et 2
24. APT IMA STD RLU Relative Light Unit 2 2 1 3 2 4 M 5 A
25. 1 3 1 HBV HCV TH 1 V pH4 pH9 300mg dL 1000mg dL 16 0mg dL 6mg dL 250Ery gL 2 VN 2 dB 0
26. 22 0 22 91 7 2 8 6 8 8 100 2 4 8 6 1 1 0 98 2 2 1 PCR 9 9 49 98 0 98 9 9 0 1 7 5 7 6 100 9 9 7 5 1 7 4 99 4 2 PCR 9 6 59 9 2 1 81 8 2 101 103 98 1 1 1 0 8 1 1 4 96 5 3 SDA 9 0 3 4 6 3 2 7 8 1 5 363 408 6 1 425 486 4 7 5 4 92 5 90 3
27. Staphylococcus aureus Staphylococcus epidermidis Streptococcus agalactiae 2 TCS a 7 3 Streptococcus bovis Streptococcus mitis Streptococcus mutans 6 WE 4 Ed 3 5 a Streptococcus oralis Streptococcus pneumoniae Streptococcus pyogenes Streptococcus salivarius Streptococcus sanguls Streptomyces griseinus v Trichomonas vaginalis Ureaplasma urealyticum Vibrio parahaemolyticus Versinia enterocolitica 3 TCS 5 6 ii i 4 400mL 2 TCS TCS
28. 3 hlamydia psittaci Chlamydia pneumoniae 1 0X10 FU a s say Herpes simplex virus 1 2 5X10 TCID as say Herpes simplex virus 2 6 0X10 5 TCTID as say Human papilloma virus 16 2 9X10 DNA copies assay Cytomegalovirus 4 8X10 infected 2 5 3 5cm cell culture cells assay 1 0X10 6 cells assay
29. 1 0 3 0 rr RE Rt 3 6
30. 6 0 5 T U BD A T TU 1 0 0 T TU U 6 2 1 2 0 1 5 30 5 T TU 2 5 0 L U U 6 2 1 1 0 TU 1 8
31. 9 RN 50 f g rRNA 3 GC r RNA 250 fF 1 3 4 8 0X103 7 2 1 E 1 IFU 50ce 1 1 s EB EBs 1 PCR 9 6 6 5
32. o ure 9 Combo 2 C TIGRIS 1000 15 30 1000 5 O 260mL 1 8
33. r RNA 1 5 3 000 000RLU 2 2 5 f g 1 CT 0 000RLU rRNA 3 3 3 2 CT 5f g 3 1 GC
34. 1 0 09 30 0 30 100 0 11 1 1 10 0 3 0 1 1 4 1 100 5 9 8 29 9 0 9 81 8 2 9 9 101 100 9 9 1 1 0 98 2 1 HBV HCV HI V i 1 000pp 2 1 1 2 1 1 2 SI 3 2 i 2
35. 2mL 2 3 0 8 2 3 0 3 0 1 5 3 0 C 3 7 5 5
36. APTIMA STD 2 3 0 3 0 3 P 22 5mg I 10 L Target Capture System A 1504L NO 1 0 2 0m
37. r RNA T7 mmmmmmmmmmmmmm DNA w 7 N RN 0 RNA RNase H Anusol 5 Vagisil 5 AA Gyne DNA Lotrimin 10 Monistat 10 RT MMLV 7 RAR RANA RRS K Y Jelly 25 Replens 10 2 TMA A TTY 25 3 Gyno1lIHN 25 i ri HPA Hybridization Protection Assay DKA Dual Kinetic Assay 1X10 mL I RNA DNA RNA
38. 1 T7 2 2 7 7mL E 6 MML T7RNA mL 1 6 P 1 2 RNN 1 0 CT PCT NGO 7 5 1 1 GC PGC NCT 1 TmL 5 1 poly A RNA r RNA po ly r RNA r RNA
39. RNA DNA RNase H NN DNA cDNA DNA DNA DNA RNA RNA Po1 RINA RNA DNA RNA 2 4 APT IMA STD T7 nw mm 5 CT GC RNase H DNA 2 1
40. NN SE 7 5 4 MTU ie MTU ER 6 2 1 1 0 4 AE 3 1 hg ER EAE Kinetic Type 5 MTU 6 2 1 1 0 RB MTU 1 0 0 0 WW 50 MT 100 000 MTU 6 2 1 1 0 MTU 4 0 1 3 8 gt RLU Relative Kinetic Type 6 Nu IRO Light Unit es 1
41. CO 3 RNA 10 0 x L MT U 4 0 0 x L MTU 1 MTU 6 2 1 3 0 MTU 40 1 20 MT U 5 1 mL MTU 2 1 mL 2 MTU 4 Kinetic Type 4
42. W 2 9L 1 1 1 TIGRIS Combo TIGRIS 250 2 LA TIGRIS 15 lt 30 Combo 2 250 250 TCR Hp IRC stmr 1 dT 4 AR 27 7mL 1 ER 11 1mL 1 1 0 PR 35 4mL 1 1 1 S 10 8mL 1 1 1 Combo 2 TIGRIS 250 B TIGRIS 2 8 250 B TCR B 2 0 6 1mL r RNAJ A ATP CTP GTP UTP dATP dCTP 3 dGTP dTTP T7 1 T7 2 T7
43. 1 5 3 0 2 BR 6 2 I 4 2 PA 5 9 RNA NA DTS 4 8 1 APTIMA STD 1 1 4 84 7 N D NR aa
44. 3 0 U Target Capture TT U 1 5 3 0 5 1 0 TCS TCS TU System TCS SO DS S U 1m TCS U TCS U TU TCS U 1 5 30 5 1 0 TCS U U TCS
45. A TIGR1S B TIGR1IS 1 250 1 McDonough S H et al High Throughput Assay for the Simultaneous or Separate Detection of Human Immunodeficiency Virus HIV and Hepatitis Type C Virus HCV Infusion Therapy and Transfusion Medicine Vol 25 164 169 1998 2 McDonough S H Bott M A Giachetti C Application of Transcription Mediated Amplification to Detection of ucleic Acids from Clinically Relevant Organisms Nucleic acid amplification technologies 113 123 Bio Techniques Books 1997 3 Verma 1 M Studies on Reverse Transcriptase of RNA umor Viruses III Properties of Purified Moloney Murine Leukemia Virus DNA Polymerase and Associated RNase HH J Virol Vol 15 4 843 854 1975 4 elson N et al Detection of all single base mismatches in solution by chemiluminescence Nucleic Acids Research Vol 24 24 4998 5003 1996 elson N et al Simultaneous Detection of Multiple ucleic Acid Targets in a Homogeneous Format Biochemistry Vo1 35 25 8429 8438 1996 6 2004 Vol 15 1 8 16 2004 7 Beem M Q et al Respiratory Tract Colonization and a Distinctive Pneumonia Syndrome in Infants Infected with Chramydia trachomatis New England Journal of Medi
46. 8 3 0 ip 1 1 6 2 2 8 3 0 3 6 5 6 ER Th 1 1 AM 7 JH 8 2 8 RI
47. GC 3 CT GC 2 Candida parapsilosis Candida tropicalis Chlamydia pneumoniae Chlamydia psittaci Chromobacteriun violaceun Citrobacter freundi1 Clostridium perfringens Corynebacteriunm genitalium Corynebacterium xerosis Cryptococcus neoformans Cytomegalovirus Deinococcus radiodurans Derxia gumnosa Eikenella corrodens Enterobacter aerogenes Enterobacter cloacae Enterococcus avium Enterococcus faecalis Neisseria meningitidis Serogroup B Neisseria meningitidis Serogroup C Neisseria meningitidis Serogroup D Neisseria meningitidis Serogroup Y Neisseria meningitidis Serogroup W135 Neisseria cinerea Neisseria dentrificans
48. 2 8 HC RLU Relative Light Unit U 5 T U TU 1 4 TU 1 5 DTS 3 7 C 5 5
49. 3 cE gt In 2 NT TUO 4 TCS 1 TU C TCS TCS 0 TCS 2 5 3 2 5 Target Capture System E 10 gt
50. 6 5 6 1 98 2 1 112 113 95 7 5 7 117 174 96 6 2 PCR 9 8 2 24 2 100 0 8 8 97 8 2 4 9 0 98 2 3 SDA 9 3 49 20 3 1 5 1 1 434 435 E 21 465 486 93 4 4 9 2 7 9 8 3 11 100 0 3 0 3 0 90 9 8 3 3 4 1 92 7 9 8 29
51. 1 62 3 507 514 2009 2 6 2 5 TEL 0120 292 832 FAX 03 5695 9234 2 62 5 Gen Probe Incorporated
52. 3 ee Target Capture system TCS 6 AS HC 1 APTIMA a i 2 204L 200x 1 000uL 2 as pe RA ND 3 CT Target Capture System GC
53. MNeisseria elongata Neisserta tava Neisseria favescens Neisseria lactamica Neisseria micosa Neisseria sicca Neisserta subfiava Neisseria perflava MNeisseria polysaccharea Faracoccus denitrificans eptostreptococcus anaerobius Peptostreptococcus productus Plesiomonas shigelloides Porphyromonas gingivalis Propionibacteriunm acnes Proteus mirabilis Proteus vulgaris Providencia stuartii Provotella intermedia Pseudamonas aeruginosa Pseudomonas fuorescens 1 8 2 8 C Enterococcus faecium Erwinia herbicola Erysipelothrix rhusiopathiae 3 Escherichia co 7 Flavobacteriun meningosepticum Fusobac terium nucleatum 2 Gardnerella vaginalis Geme
54. cine Vol 296 306 310 1977 8 Holmes K K et al Disseminated Gonococcal Infection Ann of Intem Med Vol 74 979 993 1971 1 9 2002 Vol 15 1 17 45 2004 10 MB EN Vol 43 37 44 2004 Vo1 6 40 41 2007 12 Yurika Ikeda Dantsuji et al In vitro assessment o he APTIMA Combo 2 assay for the detection of Chlamydia trachomatis using highly purified elementary bodies Journa of Medical Microbiology 54 357 360 2005 13 Transcription Mediated Amplihcation RNA Chlamydia trachomatis Meisseria gonorrhoeae Vol 15 1 116 126 2004 11 14 Combo 2 Vo
55. lla haemolysans Haemophilus ducreyi Haemophilus influenzae Haemophilus parainfluenzae Herpes simplex virus1 4 Herpes simplex virus 2 Human papilloma virus 16 Hingella dentrificans Kingella kingae Klebsiella oxytoca Klebsiella pneumoniae MTU MTU MTU Lactobacillus acidophilus Lactobacillus brevis Lactobacillus jensoni1 T i pj 0 1 p 1 t MTU Lactobacillus lactis Legionella pneumophila Leuconostoc paramensenteroides x 2 i 4 9 RB 3 Listeria monocytogenes Micrococcus luteus Moraxella lacunata 1 Moraxella osloensis Morganella morganii Mycobacteriun smesgmatis Ncoplasma genitalium Mycoplasma hominis Neisseria meningitidis Serogroup A 6 2
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