6.ノロウイルス
Contents
1. I RT PCR PCR 1 1 RT PCR Reverse Transcription Polymerase Chain Reaction RNA DNA cDNA PCR2. 2006 2007 56 10 2010 261 Tetsuya AKIBA
3. 2006 50 GMO 3 10 100 12 72 24 48 1 3 1 169 0073 3 24 1 27 29 Department Microbiology Tokyo Metropolitan Institute Public Health 3 24 1 Hyakunin cho Shinjuku ku Tokyo 262 6 2
4. ORF1 2 junction region 1st PCR Nested PCR PCR 10 Nested PCR 28 30 1st PCR GI COG1F 5 CGY TGG ATG CGN TTY CAT GA S G1 SKR 5 CCA ACC CAR CCA TTIR TAC A S 381bp GI COG2F 5 CAR GAR BCN ATG TTY AGR TGG ATG AG G2 SKR 5 CCR CCN GCA TRH CCR TTR TAC AT 3 387bp Nested PCR GI COG1F COG1R 5
5. PCR GT GI G GII G1 G2 G1 amp G2 29 31 263 5 NASBA Nucleic Acid Sequence Based Amplification 3 RNA NASBA NASBA 41 C GI GIH GI GI 6 TRC Transcription Reverse transcription Concerted reacton 43C RNA TRC
6. 1 Norovirus 1968 Norwalk like viruns small round structured virus SRSV 2002 38nm 7 500 1 RNA 1 GT GV genogroup GT GI GI 14 GI 17 geno type GL 4 2
7. POCT Point 264 6 of care Testing 7 4 HAT NV 2008 4 7 30 32 PCR 1 Kapikian A Z et al Visualization by imm
8. RNA DNA PCR 3 RT LAMP Reverse Transcription Loop mediated Isothermal Amplification LAMP DNA 1 Loopamp GI GI Loopamp GI GI 4 Ampdirect RT PCR DNA Ampdirect PCR 1
9. RNA INAF Intercalation Activating Fluores cence GT GI GI GI TRCRtest NV W 2 1 EIA Enzyme Immunoassay GT GI NV AD 2 ICA Immunochromatographic Assay EIA
10. CTT AGA CGC CAT CAT CAT TYA CS 85bp GI COG2F COG2R 5 TCG ACG CCA TCT TCA TTC ACA S 98bp 2 PCR TaqMan PCR reporter quencher RT PCR cDNA 6 RT PCR COG GI RING1 TP a 5 FAM AGA TYG CGA TCY CCT GTC CA IMRA S RING1 TP 5 FAM AGA TCG CGG TCT CCT GTC CA TIMRA S GI RING2 TP 5 FAM TGG GAG GGC GAT CGC AAT CTI TIMRA S FAM reporter TMRA quencher PCR 10
11. une electron microscopy of a 27 nmparticle associated with acute infec tious nonbacterial gastroenteritis Virol 10 1075 1081 1972 2 19 5 14 0514004 3 http wwwr tokyo eiken go jp
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