アプティマTM Combo 2 クラミジア／ゴノレア
Contents
1. Target Capture System TCS HC2. 2 3 0 Y 6 0 7 0 2 0 9 0 2 APTTIMA STD 1 1 2 2 0 3 0 mL 3 2mL
3. C DTS WM DI RS OO 9 1 5 3 0 B
4. 1 2 TTU 4 1 3 TTU MTU 3 6 1 2
5. 3 CT 5 1 2 3 4 7 GC 1 4 1 5
6. HPA Hybridization ProtectionAssay 3 2 DKA DualKineticAssay yz 8 3 AE RNA DNA RNA DNA C3 DNA
7. 7 8 2 1 MTU 1 1 CT 2 GC 3 CT GC
8. Chlamydia trachomatis CT WVeisseria gonorrhoeae GC
9. TTU TTU 1 4 TU 1 5 2 DTS 1 2 3 RNA 10 0 zL MTU 4 0 0 z MTU 1
10. 1 000ppm 1 2 1 1 2 1C 1 2 3 2 1 2
11. DTS Combo2 TIGRIS 250 A TIGRIS 1 5 3 0 550 TCR 1 RNA 1 61ng le 5 4 mL 1 RNA 2 52ng dT 25zg 4 AR 27 7mL 1 7 ER 11 1mL 1 10 PR 35 4mL 1 11 S 108mL 1
12. 4 2 3 0 8 2 3 0 3 0 4 1 1 1 5 3 0 3 7 5 5 TTU TargetCapture System
13. 2 8 C 3 0 5 6 1 1 2 8 15 3 0C 6 2 RNA DTS 4 8 2 42Cx1 62Cx2 VWR Mult
14. 2 4 cm 2 3 2 1 0 3 0
15. 2 3 4 5 CT GC
16. 3 4 5 6 7 _ 2 5 3 0 1 Kinetic Type 6 Kinetic Type RLU 1 0
17. TTU 10 0 L CY OS TTU TTU 6 2 1 2 0 TTU 1 5 3 0 C 5 TTU 2 5 0 gL TU TTU 6 2 1 1 0 TTU 1 8 2 8 1 5 HC RLU Relative Light Unit T TU
18. 3 TCS 5 6 4 0 0mL 2 TCS TCS 3 TCS TCS TC5 TCS 2 5 3 5 Target Capture System 5 2 5 3 1 0
19. APTIMA STD 2 3 0 6 0 70 20 C 9 0 APTIMA STD 2 3 0 3 0 2 1 r RNA Anusol 5 Vagisil 5 Gyne
20. Target Capture System 1 0 2 5 3 5 cm 6
21. 2 4 1 2 2 5 3 1 TCS 2 5 3 10
22. r RNA RNA 4 5 1 1 1 5 0 0 0 0 RLU 2 5 f g rRNA 100 000 RLU 3 000 000 RLU 2 5 0 f g r RNA
23. 2 TTU 7 5 TU 2 0 0 TTU TU TU 6 2 1 1 0 TTU 4 2 1 5 U TTU 2 5 U TU 4 2 1 6 0 4 2 3
24. 1 1 Combo 2 TIGRIS 250 KB TIGRIS 2 8 250 B TCR B 2 0 6 1mL 1 1 rRNA 0 50ng A 1 ATP 0 78 g mol CTP 0 14 gx mol GTP 0 96 wg mol UTP 0 14 pw mol dATP 38 nmol dCTP 38 nmol 27 7mL 3 dGTP 38 nmol dTTP 38 nmol 1 T7 1 013 gk g T7 2 01 1zg T7 1 62ng T7 2 57ng 250 E Gi 6 1 MMLV 9886U 1 T7RNA 2196U P 9 1 1 043ng 35 4mL 1 2 0Jlng 12 CT PCT NGC 1 7mL 5 13 GC PGC NCT 1 7mL 5 Combo 2 C TIGRTIS 1 0 0 0
25. 3 GC r RNA 250fg 3 4 1 IFU 50ce 1 1 s EB 8 0x10 3 EBs 7 2 1 1 PCR 9 6 6 5 6 5 6 1 9 8 2 1 112 113 9 5 7 5 7 117 174 96 6 2 PCR 9 8 2 2 4 2 2 6 1 0 0
26. PCR 9 6 5 9 2 11 8 1 8 2 1 0 1 1 0 3 98 1 1 1 103 114 96 5 3 1 0 0 3 0 0 3 0 1 0 0 0 1 1 1 0 0 3 0 1 1 4 1 10 0 4 9 8 2 9 0 2 9 9 TT 9 9 81 8 1 0 0 9 8 2 f uk uk 3 1 1 HBV HCV HIV
27. Combo 2 C TIGRIS 1000 15 3 0 1000 5 0 260mL 1 8 W 2 9 1 1 1 1 po ly A RNA rRNA p o ly r RNA rRNA 1 8 Target 3 jo Sequence
28. TTU RNA 1 0 0 1 TTU CT 2 GC 4 0 0 gL TTU 4 0 0 L 3 T TU TTU 6 2 1 3 0 TTU 6 0 2 TTU 1 5 3 0 C 3 0 TTU Target C
29. 2 3 4 3 MTU MTU MTU T ip T i p 1 et MTU Tip MTUT ip 1 6 2 C
30. Acinetobacter 3 Achromobacter xerosis Acinetobacter Iwoffi calcoaceticus c 4 Actinomyces Actinomyces 7S7g677 Aerococcus viridans pyogenes Agrobacterium Aeromonas hydrophila Alcaligenes faecalis radiobacter Bacillus subtilis Bacteroides fragilis Bacteroides ureolyticus Bifidobacterium s Branhamella Bifidobacterium brevi adolescentis catarrhalis Brevibacterium linens Campylobacter jejuni Candida albicans Candida glabrata Candida parapsilosis Candida tropicalis Chromobacterium Chlamydia pneumoniae Chlamydia psittaci Violaceum 3 Clostridium Corynebacterium Citrobacter freundii 2 2 677777ge7S genitalium Corynebacterium Cryptococcus 5 CytomegaloVirus Xerosis neotormans Deinococcus radiodurans Derxia gummosa Eikenella corrodens Enterobacter aerogenes Enterobacter cloacae Enterococcus avium Enterococcus faecalis Enterococcus faecium Erwinia herbicola Erysipelothrix 0 s Flavobacterium a Escherichia colt lt Thusiopathiae meningosepticum Fusobacterium i Gardnerella vaginalis Gemella haemolysans nucleatum Haemophilus ducreyi Herpes simplex virus 2 Haemophilus influenzae Human papilloma virus 16 Herpes simplex virus 1 Kingella dentrificans Kingella kingae Klebsiella oxytoca Klebsiella pneunmoniae Lactobacillus acidophilus Lactobacillus lactis Lactobacillus brevis Legionella pneumophila
31. 3 3 1 Combo 2 TIGRTIS 250 A TIGR1S TCR Combo 2 TIGRTIS 250 B TIGR1IS B TCR B CT PCT NGC Combo 2 C TIGRTIS 100 0 0 AR ER P R S A W 2 18 Combo 2 TIGRIS 250 A TIGRIS B TIGRIS 1 250 Combo 2 C TI
32. MTU 6 2 1 C 3 0 MTU 4 0 1C 2 0 MTU 5 1 mL TU 2 1mL TTU 2 4 2 0 0 MTU 7 5 MTU MTU 6 2 1C 1 0 MTU 4 2 1 1 5 2 5 uL MT U MTU 4 2 1 6 0 MTU 6 2 1C 1 0 MTU
33. Lotrimin 10 Monistat 10 K Y jelly 25 Replens 10 20 Ng GynolI 2 5 Delfen 19 3 0 1 0 10 2 r RNA pH4 pH9 3 Chlamydia psittaci Chlamydia pneumoniae 1 0x10 IFU assay Herpessimplexvirus1 2 5Xx10 TCID as say Herpes simplex viruns2 6 0x10 TCTD as say Human papiloma virus 16 2 9x10 DNA copies assay Cytomegalovirus 4 8x10 infected cell culturecells assay 1 0x10 cellsZassay
34. 0 8 8 8 8 9 7 8 2 4 9 0 114 98 2 3 9 2 7 8 3 1 1 10 0 0 3 0 3 0 9 0 9 8 3 3 4 1 9 2 7 4 9 8 2 2 2 0 2 2 9 1 7 0 2 8 6 8 8 10 0 2 4 8 6 1 1 0 98 2 2 1 PCR 9 9 4 9 8 0 9 8 9 9 0 1 7 5 7 6 10 0 9 9 7 5 1 7 4 99 4 2
35. 1 0 0 x LL MTU 6 2 1C 2 0 5 2 5 0 MTU MTU 6 2 1C 1 0 MTU 4 0 1C 3 8 RL U Relative Light Unit la 1 APTIMA STD 2
36. 1 5 0 0 00RLU 3 000 000RLU 2 1 2 CT rRNA 5 f g 3 3 GC rRNA 250fg 3 1 3 2 CT rRNA 5 f g 3
37. RNA RNA 1 5 3 0 3 0 1 1 2 8 3 0 1 1 2 8C 3 0 1 1 6 2
38. 3 DTS DTS MTU DTS MTU Tip Tiplet MTU Tip MTU Tip APTIMA STD APTIMA STD 3 APTIMA STD APTIMA STD APTTIMA STD 1 APTTMA STD 1 1
39. DNA cDNA DNA DNA DNA RNA RNA Pol RNA RNA DNA RNA 2 T7 mr RNA RNaseH DNA DNA 6 T7 mm DNA T7 N me DNA 100 10 0 00 6 D RT MMLV RNA pol T7 RNA 2 TMA 3 HPA Hybridization Protection Assay DKA Dual Kinetic Assay RNA DN A RN A RNA DNA
40. 3 HP A 1 1 HBV HCV HI V 2 3
41. 8 5 Capture Bead 8 Sequence Oligo 8 0 E Si b5 igo Tai S 8 AAAAAAAAAAAAAAAAA 8 O a 8 8 LL TTmmrrmrT 1 oO 6 WVVVVVVVVVVVVVVVv 0 8i8 a TT 8 8 Capture 2 TT Oligo Tail 1 iG a 2 8 8 8 6 O6 H fa 6 6 6 6 Target 3 Ue 3 Sequence equence 1 2 TMA Transcription Mediated Amplification rRNA IMMLV Moloney Murine Leukemia Virus R NA DNA RNA MMLV RNA DNA DNA DNA DNA DNA RNA RNA RNase MMLV rRNA RNA DNA RNase H RNA
42. 0 0 4 5 0 0 0 0 0 Kinetic Type Kinetic Type 1 RLU 60 000 60 000 RLU lt KineticType 2 i sks 0 KineticType 3 RLU 85 000 RLU groszso oo6 KineticType 4 85 0002 RLU lt 250 000 RLUz 250 000 Kinetic Type 5 Lb 25 0 0 0 RLU Np Bn 100 000 KineticType6 DR OE RLUz 250 000 RLU RelativeLight Uni t 2 1
43. ABD02T 2006 8 2 2006 7 1 2 TF Combo 2 IGRIS 2 50 1 2 Combo 2 C TIGRTIS 10 0 0 15 3 0 C
44. GRIS 1000 1 1000 1 McDonough S H et al High Throughput Assay for the Simultaneous or Separate Detection of Human Immunodeficiency Virus HIV and Hepatitis Type C Virus HCV Infusion Therapy and Transfusion Medicine Vol 25 164 169 1998 2 McDonough S H Bott M A Giachetti C Application of Transcription Mediated Amplification to Detection of Nucleic Acids from Clinically Relevant Organisms Nucleic acid amplification technologies 113 123 Bio Techniques Books 1997 3 Verma I M Studies on Reverse Transcriptase of RNA Tumor Viruses III Properties of Purified Moloney Murine Leukemia Virus DNA Polymerase and Associated RNase H J Virol Vol15 4 843 854 1975 4 Nelson N et al Detection of all single base mismatches in solution by chemiluminescence Nucleic Acids Research Vol 24 24 4998 5003 1996 5 Nelson N et al Simultaneous Detection of Multiple Nucleic Acid Targets in a Homogeneous Format Biochemistry Vol 35 25 8429 8438 1996 6 2002 VoL151 17 45 2004 7 Yurika Ikeda Dantsuji et al In vitro assessment of the APTIMA Combo 2 assay for the detection of Chlamydia trachomatis using highly purified elementary bodies Journal of Medical Microbiology 54 357 360 2005 8 Transcript
45. Lactobacillus jensonii Leuconostoc paramensenteroides Listeria monocytogenes Micrococcus luteus Moraxella lacunata Moraxella osloensis Morganella morganii Mycobacterium smegmatis Mycoplasma genitalium Mycoplasma hominis Neisseria meningitidis Serogroup A Neisseria meningitidis Serogroup B Neisseria meningitidis Serogroup C Neisseria meningitidis Serogroup D Neisseria meningitidis Serogroup Y Neisseria meningitidis Serogroup W135 Neisseria cinerea Neisseria dentrificans Neisseria favescens Neisseria elongata Neisseria lactamica Neisseria fava Neisseria mucosa Neisseria sicca Neisseria subflava Neisseria perflava Paracoccus Peptostreptococcus Neisseria polysaccharea 2 denitrificans anaerobius Peptostreptococcus Plesiomonas Propionibacterium productus shigelloides acnes Proteus mirabilis Proteus vulgaris Providencia stuartii Pseudomonas Pseudomonas Pseudomonas putida aeruginosa Huorescens a Rhodospirillum Saccharomyces Rahnella aguatilis 2 Tubrum cerevisiae Salmonella Salmonella minnesota 2 Serratia marcescens trphimurium Staphylococcus saprophyticus Streptococcus agalactiae Streptococcus mutans Staphylococcus aureus Streptococcus bovis Streptococcus pneumoniae Staphylococcus epidermidis Streptococcus mitis Streptococcus pyogenes Streptococcus salivarius Streptococcus sanguis S
46. apture System TCS TTU 15 3 0C 5 1 0 TCS TCS TTU TTU 1mL TCS TTU TCS OO BO SS TTU T TU TCS TU 1 5 3 0 C 5 1 0 TCS TTU TTU TCS TTU TCS 2
47. i Tube Vortexer Target Capture System TCS HC APTIMA TO EAA Target Capture System Ww Ss 20uL 200kL 1 000gL 1 250gL LL mL 12 5mL TT O 5 622 2 5 3 2 7 BB APTIMA STD APTIMA STD DTS APTIMA ig 5 6 2 5
48. ion Mediated Amplifcation RNA Camyda trachomats TNeissera gonarrhoeae VoL15 1 116 126 2004 262 5 TEL 03 5695 9210 FAX 03 5695 9234 2 62 5 Gen Probe Incorporated
49. treptomyces griseinus Trichomonas vaginalis Ureaplasma urealyticum Vibrio parahaemolyticus Yersinia enterocolitica 1 TTU 1 TTU CT 2 GC 2 5 1 8 2 8C
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