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BrdU Flow Kit

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1. FA HEIL 5 FITC BrdU Flow KitHHHHHHHHHHHHHHHHHHH OOOTO OG vitro 000000000000000 00000000000000000000 0000 450 0 p d 00000000 08rdV Flow Kit 0000000000 000000000000 DNase 0000000 000000 FTC ant Brd 107 0 FE3ITID 0 C 0000000 190000000000 000 2 OPED 0001 1000 OOO00000000000000000000000005 A1Q 4000000000000 0 0000 00000000000 080 FACSComp Bead OO 00000 0 0000000000060 5 810 B2 BD CellQuest J 0 0000000000 00000000 00000 00000000000 SAID A4HHHHHHHHHHHHHHHHPMTHHHHHHHHHHHHHHHHHH SSC vs 0000 5 5 820 30 000 7 PNAD 0 0 0 00 0 OOO000000000000000000000000000000000000 5 820 7 AADQ O FL3H HHHHHHHHHHHH5 A3HHHHHHHHHGO G1HHH 3HH HHHHH MFIHH 590000 2560 0 0 000 200 000 10240 DO 000 000 PM 00 0000 0000 00 000 0 0 HHHHHHHHHHHHHHHHHFLIHFLZHHHHHHHHHHHHHHHHHHHHHHHPMIHHH 0 0 0 000 0 0 10 1090000 00000000000 FLI os ru FL 36 05 FLI FL2 FL3 FLS voff ru Pp FLI 1 alt FLA 75 E Four 6 FITG FL1 T 0000 0 MOO06A NO00000000000000000000006 A20 A400 OO OOO OFTC ant
2. anti IL 10 vs FITC anti BrdU 0000000 OBrdug 00000 1 10 0000000 38 0 000000 01 000000000000 0 0 0 0 1 100 0 0 0 0 0 0 0 0000 0 0 0 00000 000 BALB III B d du vitro 0000000000000000000000000000000000 HH PMAHHHHHHHHHHHHHHHHHHHHHHHHHH45HHHHHH10 BrdUHHHHHH 0 FL1 IIPE anti IL 10 FL2 T 7 AAID anti CD OOO00000000000000000000000000000000000020000000000000 HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHUHHHHHHHHHHHUHUHHL Lt di EZ CE LETT n A Cr mz Del rra 2 BrdU Flow 00000000000000 IIHHHHHHHHHHHHHHHHHHHHHHHH FIC anti CD A PE anti CD8JAPC anti B220H 0000 BD Cytofix Cytoperm Buffer 0 0 0 LI 0 0 H AD BO B 000000 BD Pharmingen BrdUH 0 0 00 IlHHHHHHHHH HHHCHDHHHHHHHHHHHHHHIHHHHHHHHHHHHHHHHHHHHH 2 IIHHHHHHHHHHHHH QCD FITCH vs CD 00 0 0 0 BH 018220 vs 8 00 040 08082200 2000000 02 Brdur 0 0000000000 0000000600 positive MFI 000 BrdU 000 BrdU 00000 000000 00000 CD44 FITCQ 20 5 21 5 278 141 CD8 8 4 9 0 649 195 B220H 62 9 60 1 261 189 02020 00800 IIHHHHHHHHHHHHHHBrdUHHHHHHHHHHHHHHHHHHHHHHHHHBrdUHHH 00 000000000000000000000000000000000000000000000000 00
3. HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 00000 00 Fluorochrome conjugated anti BrdU antibody O00 0 00000 00 0000 00 BrdU 50 00 0005000000000 0000 1090 0 FITC BrdU Flow Kit 000 000 55961910 Bedug 000000 50 121 nnd 0000 BrdU Flow 0000 0 5525980 BrdU 000000 50 arm gg 000000000000 IIHHHHHH1XBD Perm Wash Buffer 1500 0 000000000 000000000000 00 SopL 00000000000 Br dU 00000 4 0 00000000 BD Cytofix Cytoperm Buffer BD Cytofix Cytoperm Buffer H OO 0 000 00000000 HHUHHHHHHHHHHHHHUHHHHUHHHHHHHHHHHHHHUHHHHHHUHHHHL HHHHHHHHHHHHHHHHHHUHHUHHHUHUHHHHHHHHHHHHHUHHHHHHHL 0 00000000000000000025 mLHHH D BD Cytofix Cytoperm Buffet 000000 0 00000 BD Cytofix Cytoperm Buffer 4 C 0 00000000 BD Perm Wash Buffet BD Perm Wash Buffer 00 0 0 T 10 25 mLHHHHHHHHHHH BD Perm Wash Buffer O0 000 00 00000000000000000000000000000 0 000000 0 00000 00 0 00 00 0000 10100 00000 0 0000 00 00 1 Perm Wash Buffer 4 0 00000000 00000 00 00 10x Perm Wash 00 200 422 0 0000000 10xBD Perm Wash Buffet 00000 00000 0 00000000000 0000000 OOOO00000000000000000000000000000 10 perm Wash Buffer 00 000 0 45 nmHHHHHHHHHHHHHHHH BD Perm Wash Buffer 1x OOOOOO00000000 00000000 00000 HUHHHHHHHUHHHHHHHUHHHHHHHUHHHHHHHHL uus GOOOOO000000000 BD Cytoperm Plus Buffer BD Cytoperm Plus Buffer BrdU Flow Kit 00000000 IlHHHHHHHHHHHHHHH2
4. BD Pharmingen BrdU Flow Kit 00000 FITC BrdU Flow Kit HHHHHHH 559619 500000 0000000 557891 4 50000 D BrdU Flow Kit HHHHHHH 552598 500000 0000000 557892 4 500 amp BD Helping all people live healthy lives 02005 Becton Dickinson and Company All rights reserved IIHHHHHHHHHHHHEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHIHHHHHHH BD BlosclencesHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 0 000000000000000000000000000000 0 0 0000000000000000000 0 0 000000000000000000000000000000000000000000 Becton Dickinson and Company I 000000000000000000000000000000000 0 0 00 BD PBD HHHHHHHHH HH Becton Dicinson and company 0 0 00 0 00 92005 BD 00000 0000000 5596190 5525980 0000 0000000000 0 Fluorochrome conjugated Anti BrdU Antibody 10000 BD Cytofix CytopermTM Buffer 17 000 BD Perm WashTM Buffer 10x0 20 000 BD CytopermTM Plus Buffer 1H 000 7 AAD 19000 e Kit Manual 0 000000000000000 4890 000000000 e BrdU 50000 e DNase 50000 5596199 552598HHHHHHHHHHHHHHHHHHHHHHHHHHH P A 9 00 BD Pharmingen BrdU Flow Kiti ao ach icr kc ira RR ne 6 Vel a se UR ro EE I EENE N 9 ee ee rekene 10 BrdU Flow Kit 0000 Rom
5. 00000 00 4900 0 00000000 0 0000000000000 Staining Buffer 1x DPBS 3 0 00000 0000 0 0 0 09 0 0 0 00000 000 000000000000 OBD Pharmingen Stain Buffel FBSO 0000 0 554656 LU ULDEIEULLL 1 56 000000 00 1000 0 m KCI 0 2g KH PO 0 2g 8 0g Na HPO4 7H5O0 pH7 2 7 4 2 16 9 000000 BD Cytofix Cytoperm Buffer 0 4 0 00 0000 00000 0000000000 R44 000000000000 00000 R43 00 0 0 0000 000000 0000000 521 00000000 5131 0000 00000 000 00 0000 0 00 0 00000 0 536 31 00000000000000 00000000 6000000000000000000000000000000000 00000000 9528000000000000000000000 BD Cytoperm Plus Buffer 0 10 00000000000 00000000000 20 21 221 00000000 000000000000 523 000 00000000000 5261 000000000 00 0000000000000 000000000 000000 5241 000000000000 000000000 00000000 34 000000 00000000 sed HH 00000000 0000 00000000000 000000 BrdUJ 0 1 0 0 0000000 00000000000 R20 21 22 0000 0000 000000000000 SH 0000000000000 0 00000000 S28 536 37 391 000000000 00000 00 0 00000000 S6JHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 0 00000004 0000000000000000000000 0 000000000 0000 00000000000000000000000000000000000000000000 000000000000 00000000 00000000 BrdU 00000000 BrdU Flow 0 00000 BrdUHHHHHHHHHHH BrdUHHHHHHHHHHHHHH7 toHHHHH in vitro 000000
6. 0 00000000 0000000 0000000 HHHHHHHHHHHHHHHH BrdU 000 0 70000 000000 a 0000000 70 BrdU Flow Kit 0000000 1 0 0 00000000 a 4000000000 1090 0 50 pL Staining O00 0 0 00000000 0000 00000 b Staining Buffer BD Pharmingen Stain Buffet FBSMNINO 554656 10 00000 0 000000000000000508401000000 000000 00000 0 00000150000 0000000 00000000 d 0000001 mL Staining Buffer 0D 000 10 0 00 02000 300 xg 500 m Hugudaututullulull 2 BD Cytofix Cytoperm Buffer 00000000000000 a 100 uL BD Cytofix Cytoperm Buffer 00000 0 00000000 b 00015030000000000000000000000000 c 1 mL 1 BD Perm Wash Buffer O0 0010 0 00 00000 1900 000 00000000 00000 3 BD Cytoperm Plus 00 0000000000 a 100 BD Cytoperm Plus Buffer 0 b 0001000 000000000 00000000 c 1ml 1x BD Perm Wash Buffer 0D 00100000 0000 20000 4 HHDBBHILU a 100 BD Cytofix Cytoperm 60000000000000000000 b 00050000 000000 000000 00000000 c 1ml 1x BD Perm Wash Buffer 0 00 10 B 0000 00002000 0 5 000000000000000 80000722 a 0 000 DNase 0 100 nH 0 0 300 pg m 00000000000000 HOW 0 00 0 0 DNasef 30 b 000100 037 00000 00000000 c 1 ml 1x BD Perm Wash Buffer 0010 00000 0000 20000 e HHHHHHHEBrdUHHHHHHHHH a 000000 Brdu HDD 0000000000 00000 50 BD Perm Wash Buffer 000000000 b HHH20HHHHHHHHHHHHHHHHHHH c 1 ml 1x BD Pe
7. 0 Flow Ki 0000000 00 00000000 0 000 0 0 0 00000 00000 00 00 00000 00000000000000000000 0 0 0 000 000000 gt BrdU Flow Kit 0 00900 0000 000000 00 0000 0 0 00 000 00 O 00 0 0 00 0 D HHHHHUHUHUHHHUHUHHHHHHHHHUHHHHHHHHHUHHHHHUHHHUHHHHHHL 00000 00 DNAD 0 0 BrdOUHHHHHHH HHHHHHHHHHHHHHHHHHHHH 0000000 0000000 00 0 00000 000000 DBrdU Flow Kit 000 D 000000 0 00000 00000 vitro 00000 0 0006 00 0 0 0000 000 0 00 0 0 00000 0 00000 0000 0000 0 0 0 0 0 0 0 0 0 0 0 00 0 0 00 0 0 000 0 00000 0 00 0 0 0 20 0000 0 0 0 0 000 00 00 BD Pharmingen BrdU Flow 00000000 000000000000000000000000 0 000000000000000000000 0 00000000 0000000 0 000000 00000000000000 000 000000000000000000000 00000000 BrdUHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHBrdUHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHBD Pharmingen BrdU In Situ 0000 0 55080305513210 0 000 BL 0 200000 0 000 0000000000 00 BD Pharmingen BrdU Flow Kit 00000 BD Pharmingen BrdU Flow Kit HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 00000000000000000000 000000000 00 10000000 00000000 0 0000 03000000000000000000 000 00000 0000 0000000000 00000000000000084000000000 84000000000000000000 0 000000000000000000000000000000000000000 000000000 00000000 0000000 C Option 1 00000000000000000000 00 0000000000000000000000000
8. 000 Option 42 0000000000 0000000000000000000000000000 BD Pharmingen BrdU Flow Kit 000000000000000000000000000000000000 0 00 BD Pharmingen BrdU Flow Kit ODO 00000 HHUHHHHHH 000 O0000000000 in vitro in vivo BrdU D 00000000 0000000000 00000 0 0 MPCH BD Cytofix Cytoperm Buffer Staining Buffer HHHHHHHHHHHH 0000 Staining Buffer 0 00000 00000 80 000 OU 010 HHUHHHHHHH Staining Buffer BD Cytofix Cytoperm Buffer 0 0 O O O DNase BrdUJ 00 0000 000 000 Staining Buffer 00000000 00000000 10 dmethyl sulfoxide DMSO TI 901 00000000 0 FBS 0000000 000 00000 00000 00000 DNase 00 D 000 Perm Wash 0 0 0 0 0000000000000000 00000 JOOOO0000000000000000000000000000000000000000 HHHHHHHHHHHHHUHHHHHHHHHHHHHHHL Total DNAHHHHHHHHHHHHHHHHHHZAADHHHHHHHHHFL3HHHHHH 000000 00000000000000 DU UB BULBEULL 1 00 0000 00000 Fluorochrome anti BrdU Antibody APC 1 BD Cytofix Cytoperm Buffer 4 C 1 BD Perm Wash Buffer 10x0 4 2 BD Cytoperm Plus Buffer ac 1 BrdU 10 mg mL 80 C 5 Dnase 80 C 5 7 AAD 4 C 1 0000000 0000 0 0 00000 00000000 00 000 0 01x Dulbecco PBS 5 BD Perm Wash Bufferll
9. 0000 80 0000 0082 26FL3HHHHHHHHHHH7 AAMFL3HIHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH OOF2000000000100000000000 10000000F0200010 lt 100 0000600 OFL3 FL20 000000000000000 0 8 810 000 9 810 0 8 4 20 00 0 anti IL 10 2 LE a a mo n TET puri 2555 1 FL1 FLZ twm a lis uar FL am of Fi K R T FLZ 44 EFLI Pa nr li ras ff zin FLJ 91 aff FL r m us iki a 1 c Lim PL i em Ba L rLa w FLS p C Celer PDH 5 0 10 FRI 00000000000000000 DOOOOO 50000000000 anti BrdU 7 AADDPE 10 2 1 10 9 PE ant TNFO HHHHHHHTINFHHHHHHHFLZHHHHHHHHHPEHHHHIL TOHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHH9 A2HHHHHH10 A2HHHHHHHHHHHHHHHHHHHHHHH OOO00000000000000000000000000000000000000000P8F2000 HHHHHHHHHHINFHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 10 20 A4HHHHHHHHHPEFL2HHHHHHHHHHHHHHHHH 7 AAMM HHHHHHHHHHHHHHHHHITNFHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHZHHHHHHHHHHHHHHHFLZH HHHHHHFL2ZHHHHHHHHHHHHHHHHHHHHEL3 6FL2H FL2 FL3HHHHHHHHHH 70 90 HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 10 820 B4 0 00 PMIH
10. 00000000000 HHHPEHHHHHHHHHHHHHHHHHFL2 6FL3H FL3 2 FL2ZHHHHHHHHHHHHHHHHHHH HHHHHHHHHH7AADHFL3HHHHHHHHHHHHHHHHHHHHHHHGO GIH 0 0 00 50000 2000 00 090900000 00000 0 00 00000 0 0 0 00000000 OFL2 FL30 vs 7 0000 Q 00000000 0000000 BrdUHHHHHHHHHHHHHHHHHHHHBD Pharmingen BrdU Flow Kit I 00000 00000000000000000000000000 00 000 00000000000 HOU 000 0000 UU 0000000000000 00000000000 0000000 0000000000000000000000000000000000 0000 00000000000000000000000 00000000 0 0000000000 000000000000000000000 0 00000000 BD Pharmingen BrdU Flow 00000000000000000000000000000000 0 00000000 000000000000000 000000 00000000 0000000 0 0000000000000000000000000000000000000 000 0000000 0000000000000000 000000000 0000000 0 OBD Pharmingen BrdU Flow Kit HB OO 000000000000 BD Pharmingen BrdU Flow kit 00000 0000 000000000000000000000000000 00000 FACSCalibur 000000000 40000 0 FACSCalibur 000 000000000 040 0000 FLIQFL2OFL30QFL40 0 00 040 000000 HHHUHUHHHUHHHHHUHHHHHUHHHH HHHHHHHHHH3HHHHHHUHHHHHHL 00000000000000000000000000000000000000000040000 0000000000000000000000000 000000 BrdUH BD Pharmingen BrdU In situ Detection JUD 0 551321 1550803 T 00 OOO 0000 IIHHHHHHHHHHHHHHHHHHHHHHHHH
11. 00000000000000000 0029088064000000000000000000000000000 00000000000000000000000000000000000000000000000000 000000000000840000000000000000 _Brdu FITC Brdu 0 3 BrdUHHHHHDNAHHHHHHHHHHHHHHHHHHHHHHHHHHHH OO0 A D10 G4 1H 000 Brdup HHH 0 0 0 L anti BrdU T DNAD 0 7 AADITI HHHD10 G4 1 00010 uMH BrdUHHHH30HHHHHHHHHHHHHHHHHHDNAHHHHHHTotal DNAD OOOO 0 0000 0000000000 00000 0 0 0 7 vs 000000000000000 0000000000 8 Flow 000 Sub GO GlQR6eG 0000000 5 6 160 6100 3038 6 0500 R438 6 2 5014 4 0 000 90 7 OOO00X0000000000000000000000 000000000 Bray ant BrdU Total 0 7 0 0 000 HHHHHCD3HHHHHHHHHHHHHHHH10 AT 0000 000 35533600 000 028 0 02 ug mLD D U 006028 40000000 555725 010 I D U U U UI IE 2 0000 0 55460300 00 20 ng mLHHHHHHHHIL HHHHHHH 5546050 20000000000000 IIHHHHHHHHHIL 2HIL 4HHHHHHH3HHHHHHHHHHHHHHHHHHHPMA SigmaHHHHH 8139 5 ng7mLIHHHHHHH SigmaHHHHHHHH 106341500 ng mL 40000000000 00 10000 020 uMQ BrdUHHHHHH3AHHHHHHHHHHHHHH6HHHHHHHH 3 31 00 40 SITI 23 5 0 G0O G1 T 64 3 0 50 G2 MH 6 1 0 00 a 40 Minutes Hours m BrdU FITC BrdU FITC Bra FIT
12. 13A5 Rat IgG FITC 0 1 mg 554544 PE 0 1 mg 554545 IL 6 MQ2 6A3 Rat 1406 FITC 0 1 mg 554696 PE 0 1 mg 554697 PE 100 Tests 559331 GM CSF BVD2 21C11 Rat 1406 PE 0 1 mg 554507 GRO 10G4 1 Mouse IgG x 0 1 555042 IFN y B27 Mouse IgG FITC 0 1 mg 554700 PE 0 1 mg 554701 PE 100 Tests 559327 APC 0 1 mg 554702 Alexa Fluor 488 100 Tests 557718 Alexa Fluor 647 100 Tests 557729 IFN y 4S B3 Mouse IgG x FITC 0 1 mg 554557 PE 0 1 mg 554552 PE 100 Tests 559326 IL 8 G265 8 Mouse IgG gt b FITC 0 1 mg 554719 PE 0 1 mg 554720 IL 10 JES3 9D7 Rat IgG PE 0 1 mg 554498 PE 100 Tests 559337 Alexa Fluor is a registered trademark of Molecular Probes Inc Eugene Or Description Clone Isotype Format Size Cat No Human Cytokines and Chemokines Continued IL 10 JES3 19F1 Rat IgG PE 0 1 mg 554706 PE 100 Tests 559330 APC 0 1 mo 554707 IL 12 p40 p70 C11 5 Mouse IgG FITC 0 1 mg 554574 PE 0 1 mg 554575 PE 100 Tests 559329 0 1 mg 554576 1 12 p70 20C2 Rat 1961 PE 0 1 mg 557020 PE 100 Tests 559325 IL 13 JES10 5A2 Rat IgG PE 0 1 mg 554571 PE 100 Tests 559328 IL 16 14 1 Mouse IgG K PE 0 1 mg 554736 IP 10 6D4 D6 G2 Mouse IgG PE 0 1 mg 55049 MCP 1 5D3 F7 Mouse IgG PE 0 1 mg 554666 100 Tests 559324 MCP 3 9H11 Mouse IgG PE 0 1 mg 555033 MIG B8 11 Mouse IgG K PE 0 1 mg 555039 MIP 1 11A3 Mouse IgG PE 0 1 mg 554730 RANTES 2D5 Mouse IgG PE 0 1 mg 55
13. 3 5761 BD Biosciences Tel 03 5805 9960 E Mail tech cell amp bd com 0120 7099 12 exe 0120 4890 77 E Mail tech_cell bd com 66 022 01 RO 0704 000 5 287
14. 4732 PE 100 Tests 559322 Thioredoxin 2G11 TRX Mouse IgG FITC 0 1 mg 559968 TNF MAb11 Mouse IgG FTC 0 1 mg 554512 PE 0 1 mg 554513 PE 100 Tests 559321 APC 0 1 mo 554514 LT Q TNF D 359 81 11 Mouse IgG PE 0 1 mg 554556 Mouse Cytokines and Chemokines IL 1 ALF 161 Hamster IgG PE 0 1 mg 559810 IL 2 JES6 5H4 Rat lgG gt b FITC 0 1 mg 554427 PE 0 1 mg 554428 APC 0 1 mg 554429 IL 3 MP2 8F8 Rat IgG PE 0 1 mg 554383 IL 4 BVD4 1D11 Rat IgG gt b PE 0 1 mg 554389 IL 4 11B11 Rat IgG PE 0 1 mg 554435 APC 0 1 mg 554436 IL 5 TRFK5 Rat IgG FE 0 1 mg 554395 APC 0 1 mg 554396 IL 6 MP5 20F3 Rat IgG PE 0 1 mg 554401 IL 10 JES5 16E3 Rat IgG FITC 0 1 mg 554466 PE 0 1 mg 554467 APC 0 1 mg 554468 IL 12 p40 p70 C 15 6 Rat IgG PE 0 1 mg 554479 APC 0 1 mg 554480 IL 17 TC11 18H10 Rat IgG PE 0 1 mg 559502 GM CSF MP1 22E9 Rat IgG PE 0 1 mg 554406 IFN Y XMG1 2 Rat IgG FITC 0 1 mg 554411 PE 0 1 mg 554412 APC 0 1 mg 554413 MCP 1 2H5 Hamster IgG PE 0 1 mg 554443 Description Clone Isotype Format Size Cat No Mouse Cytokines and Chemokines Continued TNF MP6 XT22 Rat IgG FITC 0 1 mg 554418 PE 0 1 mg 554419 APC 0 1 mg 554420 TNF TN3 19 12 Hamster IgG PE 0 1 mg 559503 Rat Cytokines and Chemokines IL 4 OX 81 Mouse IgGi PE 0 1 mg 555082 IL 10 A5 4 Mouse IgG gt b PE 0 1 mg 555088 IFN g DB 1 Mouse IgGi FITC 100 Tests 59498 PE 100 Tests 59499 GM CS B61 5 Mouse IgG PE 0 1 mg 555092 1 2H5 Hamster IgG PE 0 1 mg
15. 554443 TNF TN3 19 12 Hamster IgG PE 0 1 mg 259503 Pig Cytokines and Chemokines IFN y P2G10 Mouse IgG PE 0 1 mg 559812 Recombinant proteins useful as specificity controls for intracellular cytokine flow cytometry Description Format Size Cat No Human IL 2 Standard 10 ug 554603 IL 3 Standard 10 ug 554604 IL 4 Standard 5 ug 554605 IL 5 Standard 5 ug 554606 IL 6 Standard 10 ug 550071 IL 8 Standard 20 ug 554609 IL 10 Standard 5 ug 554611 IL 16 Standard 5 ug 554637 GM CSF Standard 10 ug 550068 MIP 1 Standard 10 ug 554622 TNF Standard 10 ug 554618 LT a TNF b Standard 10 ug 554619 Mouse IL 2 Standard 20 ug 550069 IL 3 Standard 10 ug 554579 IL 4 Standard 10 ug 550067 IL 5 Standard 5 ug 554581 IL 6 Standard 5 ug 554582 IL 10 Standard 10 ug 550070 GM CSF Standard 10 ug 554586 MCP 1 Standard 5 ug 554590 TNF Standard 10 ug 554589 Rat IL 4 Standard 5 ug 555107 IL 10 Standard 5 ug 255113 GM CSF Standard 5 ug 555111 1 Standard 5 ug 555110 Intracellular Cytokine Positive Control Cells Description Cytokines Expressed Cat No Human HiCK 1 Positive for IL 2 IFN y TNF 555061 HiCK 2 Positive for IL 3 IL 4 IL 10 IL 13 GM CSF 555062 HiCK 3 Positive for IL 1 IL 1 IL 6 IL 12 TNF 555063 HiCK 4 Positive for IL 8 GRO a IP 10 MCP 1 MCP 3 MIG MIP 1 555064 Mouse MiCK 1 Positive for IL 2 IFN y TNF 554652 MiCK 2 Positive for IL 3 IL 4 IL 10 GM CSF TCA3 554
16. 653 MiCK 3 Positive for IL 1 IL 6 IL 12 MCP 1 TNF 554654 Rat RICK 2 Positive for IL 4 IL 10 GM CSF IFN y TNF 555094 Description Clone Format Size Cat No Isotype Controls Mouse 1961 21 0 1 mg 554679 PE 0 1 mg 554680 100 Tests 559320 0 1 mg 554681 Mouse lgG2a k 155 178 FITC 0 1 mg 554647 PE 0 1 mg 554648 PE 100 Tests 559319 Mouse IgG2b Kk 27 35 FITC 0 1 mg 555057 PE 0 1 mg 555058 Rat 1961 R3 34 HTC 0 1 mg 554684 0 1 mg 554685 PE 100 Tests 559318 APC 0 1 mg 554686 Rat IgG2a R35 95 FITC 0 1 mg 554688 PE 0 1 mg 554689 PE 100 Tests 559317 APC 0 1 mg 554690 Rat IgG2b A95 1 FITC 0 1 mg 556923 PE 0 1 mg 556925 APC 0 1 mg 556924 Hamster IgG 235 2356 0 1 mg 554711 Intracellular Cytokine Flow Cytometry Kits and Reagents Description Cat No Kits BD Cytofix Cytoperm Kit 554714 BD Cytofix Cytoperm Kit with BD GolgiStop 554715 BD Cytofix Cytoperm Kit with BD GolgiPlug 555028 Human Intracellular Cytokine Staining Starter Kit 559302 Mouse Intracellular Cytokine Staining Starter Kit 559311 FITC BrdU Flow Kit 559619 BrdU Flow Kit 552598 Description Clone Format Size Cat No Related Flow Cytometric Reagents and Buffers anti BrdU 3D4 Purified 0 1 mg 555627 anti BrdU FITC Set 100 tests 556028 anti BrdU PE Set 100 tests 556029 BD Cytofix Cytoperm Buffer 125 mls 554722 BD Perm Wash Buffer 10x 100 mls 554723 BD GolgiStop contai
17. 84000000000000000000000000000000 0 2400 0 00010 00110000012 10 pp 00000 0 00000000000 0000 OO000000000000000000000000000000000000000000 720000 BrdUHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 00000000000000849000000000000000000000000000000000 000000 0 0 0000000000 0 0 0 zn vitro I O O00 0000 0 00 1 10 nL BrdU 1XDPBS 1 mM BrdU 0 00000000000000000000000000 0 00000000 0000 HHHL OOO00000000000000000000000000000002x100 0 2000000 0000000000000000000000000000000000000 0 00000000 0 00000000 00000000 0000 00000000000000000000000 0 0 0 0 200 00000000000000000000000 300 45 GARRETT HHHUHHHHHHHUHHHHUUUHHHHHHHHHHHHHHHHUHHHUUHHUHHHHHHHL 0000 BrdUHHHHHHHHHHHHHHHHHHHHHHHHHHHH 8490000000000000 0000000000000 0000000000 in vivo 0000 in 00008400000000000000000008 400000000000000000 HHHHHHHHHHHHBrdUHHHHHHHHHHHHHHHH 00 Biosciences Pharmingen 0 00000 000000000 000 00000 0 00000000 Brdug 0 000 1x DPBS 0 BrdU 0 10 mg mL zz vivo 0 BrdU O 1000 200 10 2 0000000000000 BrdU 000 00 0000 00000000000 00000000 00 400000008 0000 00 0 8 mg mL 10000 00000 00000 000 00 0000 000000000 00000 520840 J 000 00 0 000 0 0 00 0 0 00000 1400 000 00 BrdU 000 HHHHHHHHHHHHHHHHH HHHHHHHHHHBrdU 00 0000 0000 0000 00000000
18. C BrdU FITC Brdu Brdu ne ete T4AAD 7 AAD 7 440 04 0000000 7 vivo Barn I 0 00000000000 57 6 1 mog 0000000000000000 C0 0000 0 400 0 020 00 0000 400 HHHHHHHHHHHHHHHHHHHBrdU 7AADHHHHHHHHHAHHHHHHHHHHH 0 4000000000008rd07AAD0 0000 00000000000000000B000000000200 HHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHBrdUHHHHHHHHHH GO G1H HH 7AADHHHHHHHHHBrdUHHHH HHHHHHHHHCHHHHHHHHH4HHHHHHHH OO0000000000060 GI008d0000000000000000000BdU7AAD 000000 HUHHHHHHHH 0000000000000 00000000 HHHHHHHHHHHHHHHHH ET PLA rer EET 2 88 0 PLS HHHHHHHHHHHHHILL2 6FL3H IIHHHHHHHHHHHHHHHHHHHHHHHH 50 15HHBrdUHHHHHHHHHHHHHH IIHHHHHHHHHHHHHHHHHHHHHHHHH 50 100 BD Pharmingen FITC BrdU Flow 0000 0 00 55961900 0 000 110 150 BD Pharmingen APC BrdU Flow 0000 OG 5525280 JO0O000000000000000000000000000000000000 OOOGGOO00000000000000000000000000000000000000000 0000 00000000 0000 000000000000000000000000 00000 00000 22770 OOO 000000000000000 00000000 000000 Howard M Shapiro Practical Flow Cytometry 3rd Edition Wiley Liss New Yorki FITC BrdU 1 0000000 m Pi Gur FL 12 FL1 rz uofsris na 2 FL3 1 sq T FL
19. HHHHHHHHHHHBrdUHHHHHHHH 00000000000000 000 8d0000000000000000000000000000000 OOO00000000000 000 0000 0000 Brdug 0 00000 0 0 0 0 I 00000 0000000000000000000000000000000008 Pharmingen BrdU In situ Detection Kit WOO 0000000 000000 Bedun I QD a 0 0 U UD BO Q 00000 HHHHUHHHHIHUHHHHHHUUHHHHHHBDRetrievagenAHHHHHUHHHHHHHHHHHHHH 0 00000000000000000000000000000000000000 00000 000000000000000000000840000000000000000008 4000000 0 0000000000000000000000000000000000000 0000000 00000000000000000 00000000 0000000 00000000000000000000 000000 00000010 000000 uus 00 00000000000 0000000 0 0 0 0 01000 000 0 O 00000000000 0000 00000000000000000000000000000000000 0000 20 100 1 0 00000 0 000 0000 000 0 0 000 000000000 0 0 0 00 0 00000 00000 Description Clone Isotype Format Size Cat No Human Cytokines and Chemokines IL 10 364 3B3 14 Mouse PE 0 1 mg 554561 IL 2 MQ1 17H12 Rat 1406 FITC 0 1 mg 554565 PE 0 1 mg 554566 PE 100 Tests 559334 APC 0 1 mg 551383 IL 3 BVD3 1F9 Rat IgG PE 0 1 mg 554676 IL A MP4 25D2 Rat IgG FITC 0 1 mg 554484 PE 0 1 mg 554485 APC 0 1 mg 554486 Alexa Fluor 488 100 Tests 557727 Alexa Fluor 647 100 Tests 557738 IL 4 8D4 8 Mouse IgG PE 0 1 mg 554516 PE 100 Tests 559333 IL 5 TRFK5 Rat IgG PE 0 1 mg 554395 APC 0 1 mg 554396 IL 5 JES1 39D10 Rat 146 PE 0 1 mg 554489 PE 100 Tests 559332 IL 6 MQ2
20. HHHHHHHHHHHH 1 4 x Four Calor 13 PHFL2HHHHHHHHHHHHHHHHHHHHHH PE FLA 00 0000000000 0 20 30 0 anti Ter 118 0 553673 7 antiBrdUHFITCHHHHHHHHHHHHHHHHHHHHHHHHHHHH IIHHHHHHHHHHHHHHHHFL3 6FL2HHHHHHHHHHHHHHHBrdU77AADHHH 00 0 0 1400 00 ris ong E rii FL raf FL x riz FL w sta ma Foer Coler Param TEU 1 14 PFL2HHHHHHHHH 14 A3H 13 A3HHHHHHHHHHHHHFL3 6FL2HHHHHHHHHHHHHHPEHHHHHHHPEHHH HHHHHHHHHHHHHHHHHHGO G1H 050 HG2 MHHHHHHHHAPC ant BrdUH7AADH 0000 vs 14 A4 ni NULL ni sz fix nz vals res ms ns Fia e r a Calor 15 FTA FLAN 00000600 FITC anti CD11B 00000 OG 557396 anti Ter 118 00 0 0 0 00 5536730 7AADHAPC ant BrdUHHHHHHHHHHHHHHFITCHHHHHHHHHFL2HHHHHHHHHHHHFL2 26FLIHHHHHHHHHHHHHHPEHHHHHHHPEHHHHHHHHHHHHHHHHHFITC vs 00000000000000000000000000000000000000
21. HHHHHHHHHHHHH 100 BD Cytoperm Plus Buffer 10 000 O 10 000 00 004 cO 0 00000000 BD Cytoperm Plus Buffer D 0000 000000000000000000000 0 000000000000000000000000000000 BrdU 0000000 01 DPBSO 0 0000 10 mg mL BrdU 0 32 5 0 5 mLO OOOO BrdU D 0 000 000 0 22 nmHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHUHHHHHHHHHHHHHHHHHUHHHHHHHHHHHH1mMHHHUHHHHHD in vitro 000 000 0 0000000000000 zzoHHHHUHUHUHHHHHHHHHU11H D vivo 0000 00000000 00000 vitro O0 0000000 0000 00 0 0 0 10 mg mL BrdUH B1 01 ml 1 x 3200 D 0001 mMHHH HU 0000000 1 10 pL mE C E D 0 00000000 00 0 0 00 10 0000 BrdU 0000 307 10 BrdU 0 50 000 0000 80 CD 0 00000000 000 0 0 40000 00000000 00000000 0 0 0 000 0 0 00 5 0000000 01 DPBS 0 000 1 mg mL DNasel D D 300 pp HB D 0000 1000 D HHHHHHHUHHHHHHDNaseHHHHHHHH1HHHHHHH700 ply 1XDPBSH 0 300 pgmLHHHHUHHHHHHHHHL DNaselHHHHHHHHH10UHHHHHHH1mgmL DNase 30 1 000000 0000 0000 1 mg mL DNasq 80 C 0 00000000 DNase 00 W 1 mg DNase mL 15 HH 000000000000000000000 100 0000 0 0 0 000 1090 000000 30 37 0000 000000 0000 DNase 50000 000 0000 8 00 0 0 0000 00 0 7 AAD 7 amino actinomycin ID 7 AADIT 0000 00000000 0 0 0 bNAD 000000000 0000 000 0 10 0 0000 0 0 000 020 abr 7 AADO 0 0000 7 AAD 10 0 0 0000 000
22. HHHHHHUHUHHHFL2 FL FL1 6FL2HHHHHHHHHHUUHHHHHHL APC BrdU Instrument Set up ir n x m rs oof af X rL x Foer Color bim Param Fui gt 011 000000000 ant BrdU APCO 00000000000 02 50 000 anttBrdUHHHHHHHHHHHHHHHH HHHHHHHHFITCH 0000000000 0 0 0 00 0 0 7 AADH ant BrdUH 0000000000 ant BrdUHHHHHHHHHHHHHHHHHH HHH11 A1THHHHHHHHHHHHHHHHHHHHHHHHHSSC Vs FSCHHHHHHHHHHHFL3HHH 7 OOO00000007000000000000011 820 0 000 11 A40 Aer d 0000 0840 0 0 D U 00 00000 00000 0 00 0100 000 0000 000 11 C1H 11 A49 0 060 610 025600 000000000 500102400 00 00 0 0 20000 0 00000 7AADH 000 D 00000 0 m n ED x r2 a f x dd d Lig x FL 1 00 Lin p 558 H usa Four Cedar Param 12 FL4HPMIHHHHHHHHHHHHHHHHHHHHHHFL2HHHHHHPEHHHHHHHHHHHFL4H HHHHHHHHHHHHHHHHHFL4 6FL2HHHHHHHHHHHHHHHHHHHHHHHHHHHHH FL2 2FL3HHHHHHUHHHHHHHHHHHHHHHHHHHHFLZHHHHHHHHHHHHHHHHHHHH FITCH PEHHHHHHHHHHHHHHHHHHHHHHHHHHFL2 6FL3HHHHHH1 4HHHHHHHHHHH 11 2 5 12 2 FL2HHHHHHHHHHHHH7AADHHHH
23. ed staining method for the simultaneous flow cytofluorometric analysis of DNA content S phase fraction and surface phenotype using single laser instrumentation Cytometry 13 60 67 Sasaki K S Adachi T Yamamoto T Murakami K Tanaka and M Takahashi 1988 Effects of denaturation with on the immunological staining of bromodeoxyuridine incorporated into DNA Cytometry 9 93 96 Lakhanpal S N J Gonchoroff J A Katzmann B S Handwerger 1987 flow cytofluorometric double staining technique for simultaneous determination of human mononuclear cell surface phenotype and cell cycle phase J Immunol Meth 96 35 40 Houck D W and M R Loken 1985 Simultaneous analysis of cell surface antigens bromodeoxyuridine incorporation and DNA content Cytometry 6 531 538 Moran R Z Darzynkiewicz L Staiano Coico and M R Melamed 1985 Detection of 5 bromodeoxyuridine BrdUrd incorporation by monoclonal antibodies role of the DNA denaturation step J Histochem Cytochem 33 821 827 Holm M M Thomsen M Hoyer and P Hokland 1998 Optimization of a flow cytometric method for the simultaneous measurement of cell surface antigen DNA content and in vitro BrdUrd incorporation into normal and malignant hematopoietic cells Cytometry 32 28 36 Mehta B A and V C Maino 1997 Simultaneous detection of DNA synthesis and cytokine production in staphylococcal enterotoxin B activated CD4 T lymphoc
24. i BrdU 0000000000000 0 000 0 0 0 08 gt 10 00 00 00 0 500 00 80 000 HHHHHHHHHHFL2 FLTHHHHHHHHHHHHHFL2HHHHHHHHHHHHHHHHHHH 6 A2 0000000000000 0000000000 0000000000 0 00000000000000 0 00000 FL 3 FLA 174 um 9 FL4 Gites osar apr verse 7 7 ADD FL300 0000 000 07 0 0000000000000 2 30 00000000 50 HHHHHHHHHHHHHHHHHHHHHHHHHH7A2ZHHHHHHHHHHHHHHFLZHHH 10 MFIS10HHHHHHHHHFL2 6FL3HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 00000000 0000000000000000 00000 FL1 E FL FLZ saoi FLI FLZ EJH x FLS FLS oof FLJ caf 4 H x Fist 08 PHFL2HHHHHHHHHHHHHHHH OOOO anti IL 10QFITC anti BrdU 7 AAD000000000000000050000000000PE lgHHHHHHHHHHHHHHHHHPMTHHHHHHHHHPE ant IL 10HHHHHHHHHHHHHHHHH OOO0000000000000008 A1Q Ag Peam Male Ree Fm LE ra WWE OG rar Um er me FL1 FL PLZ mi BEL ama i TE zi d I ra Es pre FL3 x FL so ma 4 Hm PT Lin FLJ 1 PO Fis Tar BEA Pores lid 5 09 PHFL2HHHHHHHHH 0 00 OFL2 FLB 10 FL3 FL2 200 0 0000 10 100 0
25. mr mrt lt 11 As I DEDE ppp n gt 11 B BrdU How 12 HH HPIY serende dos EE 13 TO DRIED sao cater arc trn datae artt aa 17 _ __ 29 DLE cesset n ____ ________ __________ 30 0000 0 0 0 Bromodeoxyuridin Brdu 00 00000000000 0 0000 0000000000 000 0000000 00000000000000000000 000000000000 Brd 50 HHHHHHDNAHHHHHHHHHH DO 00000 0 Brdug ant BrdUU D U U 0 00 IIHHHHHHHHHHHHHHHHHHH BrdUHHHHHHHHHHHHHHHHHH 7 amino actinomycin Ip 7 000 00000 0 0 000 BrdUHHHHHHHHHHHHHH 0000 00 0000000000000 21000000 0000000 0 0000000000000 00 DU 7 0000 000 0 6040 050000 G2 M DU 00 T BrdU 0 00000000000000000000290 HHBrdUHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HIHHHHHHHHHHHHHHHHHHHHHHHHHHBrdOHHHHHHHHH 0000000000 00000000000000000000008400000000000000000000000000 000 0000 00000 000 0 00 vitro m viv 0000000 00000000 HHHHHHHHHHHHHUHHHHHHHHHL BD Pharmingen BrdU Flow KitllHHHHHHHHBrdOUHHHHHHHHHHHHHHHHHH HHHUHHHHHHHHUHHHUHHHHHHHHHHHHHHHHHHUHHHHHHHUHHUHHL HHHHHUHHHHUHHHHUHHHHUHHHUHUHUHUHHUHHUHHHHUHHHHUHHUHHHHHHHHL 0 0 00000000000000000000000000000000000000000000 000000 084
26. ning monensin 0 7 mL 554724 BD GolgiPlug containing brefeldin A 1 0 mL 555029 BD Cytofix Buffer 100 mls 554655 BD Pharmingen Stain Buffer FBS 500 mls 554656 BD Pharmingen Stain Buffer BSA 500 mls 554657 BrdU Solution 25 0 mg 550891 7 AAD Staining Solution 2 0 mL 559925 Propidium lodide Staining Solution 2 0 mL 556463 Related BrdU Products BD Pharmingen BrdU n Situ Detection Kit 50 tests 550803 BD Pharmingen BrdU n Situ Detection Kit II 200 tests 551321 1 Sasaki K T Murakami and M Takahashi 1989 Flow cytometric analysis of cell proliferation kinetics using the anti BrdUrd antibody Gan To Kagaku Ryoho 16 2338 2344 Miltenburger H G G Sachse and M Schliermann 1987 S phase cell detection with a monoclonal antibody Dev Biol Stand 66 91 99 Vanderlaan M and C B Thomas 1985 Characterization of monoclonal antibodies to bromodeoxyuridine Cytometry 6 501 505 Gratzner H G and R C Leif 1981 An immunofluorescence method for monitoring DNA synthesis by flow cytometry Cytometry 1 385 393 Lacombe F F Belloc P Bernard M R Boisseau 1988 Evaluation of four methods of DNA distribution data analysis based on bromodeoxyuridine DNA bivariate data Cytometry 9 245 253 Dean P N F Dolbeare H Gratzner G C Rice and J W Gray 1984 Cell cycle analysis using a monoclonal antibody to BrdUrd Ce Tissue Kinet 17 427 436 Toba K E F Winton and R A Bray 1992 Improv
27. rm Wash Buffer 0010 HH HH EH 0000 20000 7 HHHUHHHHHHHHHHHHHDNAHH DNAHHHHHHHHHHHUHHHHHHHH 81 D 0000000 a HHH20nLH 7 AAD0 00000000 e 00000000000000000 a 1 mL Staining 0 000000000000000000 b 000000000 00000000000 4000 0 000000 2000000000000 000 0000 000 0000000000 0 O 0 0000000000 4 00000 100 0 00000 00000 0000000000000000000000 0000000000000000000 0488 DB BO UU 000000 00000000 0 00 fluorescein isothiocyanat FITC FLIM phycoerythri FL2 7 AA FL3 0 0 000000 009 000 000 000000 000000000000000000000000000000000000000 0 000 allophycocyanit 000 000 IBD FACSCalibur Dr 00 00000000 000000000000 000000000000000000000000000 0000000 000000000000000000000 00000000 0000 0000 0 0000 0007 000000000000000000000000000000000000000 0 00000000000000000000000 BD Pharmingen FITC BrdU Flow 00000000000000 0 0 7 AAD vs FITC anti BrdU DOO 0000 0 000 000 00 0 0 000000 50 bNAD 0 0 0 00000000000000000000 Quadrant Marker 0 0 00000000 027 0 000 0 00 000 000000 T 0000 B anti CD8 vs FITC anti BrdU 0000000 000 00 8 2400 000000 000 Q APC anti CD8 vs PE anti IL 10 IlHHHHHHHIL 10H 0000000000000 080 0 E O 0 347 IL 10J 000000
28. ver rates of mature lymphocytes Eur J Immunol 20 1697 1708 Westermann J S Ronneberg F J Fritz and R Pabst 1989 Proliferation of lymphocyte subsets in the adult rat a comparison of different lymphoid organs Eur J Immunol 19 1087 1093 Robey E D Chang A Itano D Cado H Alexander D Lans G Weinmaster and P Salmon 1996 An activated form of Notch influences the choice between CD4 and CD8 T cell lineages Cell 87 483 492 Carayon P and A Bord 1992 Identification of DNA replicating lymphocyte subsets using a new method to label the bromo deoxyuridine incorporated into the DNA J Immunol Meth 147 225 230 Gonchoroff N J J A Katzmann R M Currie E L Evans D W Houck B C Kline P R Greipp and M R Loken 1986 S phase detection with an antibody to bromodeoxyuridine Role of DNase pretreatment J Immunol Meth 93 97 101 Shapiro H M Practical Flow Cytometry 3rd Edition Wiley Liss New York Gray J W and Z Darzynkiewicz Eds Techniques in Cell Cycle Analysis Humana Press Clifton New Jersey Note Note T 960 2152 1 107 0052 4 15 1 www bd com jp BD Is 0120 8555 90 Fax 024 59
29. ytes by flow cytometry J Immunol Meth 208 49 59 Endl E P Steinbach Kn chel and F Hofstadter 1997 Analysis of cell cycle related Ki 67 and p120 expression by flow cytometric BrdUrd Hoechst 7AAD and immunolabeling technique Cytometry 29 233 241 Dolbeare F H Gratzner M G Pallavicini and J W Gray 1983 Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine Proc Natl Acad Sci USA 80 5573 5577 Penit C 1986 in vivo thymocyte maturation BrdU labeling of cycling thymocytes and phenotypic analysis of their progeny support the single lineage model J Immunol 137 2115 2121 Thoman M L 1997 Early steps in T cell development are affected by aging Cell Immunol 179 11 123 Tough D F and J Sprent 1994 Turnover naive and memory phenotype T cells J Exp Med 179 112 1135 von Boehmer H and Hafen 1993 The life span of naive alpha beta T cells in secondary lymphoid organs J Exp Med 177 891 896 20 PAIN 22 23 24 Zo 26 27 Schittek B K Rajewsky and l Forster 1991 Dividing cells in bone marrow and spleen incorporate bromodeoxyuridine with high efficiency Eur J Immunol 21 235 238 Rocha B C Penit C Baron F Vasseur N Dautigny and A A Freitas 1990 Accumulation of bromodeoxyuridine labeled cells in central and peripheral lymphoid organs minimal estimates of production and turno

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