ウムラック AT- - ProteinPurify 蛋白精製工業
Contents
1. 1 2 P12. 0 1 10ml 0 23m1 30 121 C15 3 AF 2 2 AA 4 5 DMSO
3. 80 C 1 96 1 1 Yoshimitsu Oda Sei ichi Nakamura Iwashiro Oki Takeshi Kato and Hideo Shinagawa Evaluation of the New System zzzz test for the Detection of Environmental Mutagens and Carcinogens Mutation Research 147 219 229 1985 2 G Reifferscheid J Heil Y Oda R K Zahn A microplate version of SOS umur test for rapid detection of genotoxins and genotoxic potentials of environmental samples Mutation Research 258 215 222 1991 3 Georg Reifferscheid Jirgen Heil Validation of the SOS zzz test using test results of 486 chemicals and comparison with the Ames test and carcinogenicity data Mutation Research 369 129 145 1996 Basal sn Level 4 1 umu p30 43 11
4. Al umurtest AFT DNA zzzz test DNA SOS zzzz galactosidase 4mes
5. 4zzes 1993 1997 DIN 38415 3 2000 SO18829 AT AT umu NM2009 5 umu test NM2009 1vial 1lml vial 89 1vial 1 1ml yial
6. 2 AF 2 9 gm1 2 AA 300 gm 100 DMSO 10 100u 17900u DD 1096DMSO 10 DMSO ATF 2 3 100 200 1 2 AA 10 100 17900 uu 1 AF 2 0 9 0 3 0 1 0 033 0g g ml 2 AA 30 3 0 3 0 03 0 003 0 kg g ml Step3 8S9Mix 89 I 1ml S9Mix A 109 Step3 S9 Mi B i S9 S9 B Step5 1 10 7 1 well 100 1 well 100z1 well Step 10zwe 37C2 37 C1 3 Step1
7. AF 2 9 g ml DMSO 0 5ml vial 2 AA 300g7ml DMSO 0 5ml vial 10 DMSO 13ml vial X gal er 11ml vial eeee 11ml vial 96well 6 1 PP 37C 610nm 670nm S 9 I Step1 7 Step2 1 10 DMS0 10096DMSO J
8. S9 89 S9 S9 S9 DMSO DMSO
9. 5 umu 17 53 59 6 Yoshimitsu Oda Hiroshi Yamazaki Masahiko Watanabe Takehiko Nohmi Tsutomu Shimada Highly sensitive umu test system for the detection of mutagenic nitroarenes in Salmonella thphimurium NM3009 Having high acetyltransferase and nitroreductase activities Environment and Molecular Mutagenesis 21 357 364 1993 7 Yoshimitsu Oda Hiroshi Yamazaki Masahiko Watanabe Takehiko Nohmi Tsutomu Shimada Development of high sensitive umu test system rapid detection of genotoxicity of promutagenic aromatic amines by Salmonella typhimurium strain NM2009 possessing high acetyltransferase activity wl lt Mutation Research 334 145 156 1995 eC OT a 8 AT umu 27 2 105 113 2004 0 Basal Level I I vV gt 1 II IV 379 2203 152 1 TEL 0270 20 857
10. Step6 37 C 2 10041 well 620nm Step7 galactosidase 1 1001well 1 570nm 2 37 1 3 100 well 2 3 620nm 610 670nm DNA
11. 2 10u1 well 1 101 hk E well Step3 8S9Mix 89 I Step4 1 S9 Step1 2 S9 2 AA S9Mix 10 S9Mix 5ml S9Mix 0 5ml Step5 Step4 1001 well
12. 2 10 10 DMS0 37 1 10 1096DMS0 EY AF 2 0 9 0 3 0 1 0 033 0 g ml 3 2 AA 30 3 0 3 0 03 0 003 0gg ml 10 AF2 AA 9zg ml 300pg ml 37C Step1 0 5ml 37 C 1 Step2 1
13. 1 FAX 0270 20 8572 PS http wwwr pro purifyrco jp
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