網 赤 血 球成熟 物質 に関す る研 究
Contents
1. 0 25M 18000g 60 ARIK y CAL I2. 26 28 V 1 0 25M 3 2 I 1 1 2 Seno S Kawai K Kanda S et al Mie Medi J Vol IV Suppl 35 43 1953 3 2 25 72 25 4 Seno S Kawai K and Nishikawa K lbid Report II 5 Plum C M Acta physiol Scand 4 259 1942 6 Jacobsen E Plum C M Acta Physiol Scand
3. Fig 3 RE RABE LSA 2 BHR DO ORE HZ 0 85 2 Ri Fig 5 8 ORADE y
4. 20cc Supernatant 3 1 pH5 4 Lick ERRAKORE CE BODIZOU CHDK 2 2 1 Grassman 110V 8 5 0 C Veronal Veronal Soda pH 8 6 880 H v0 1 Carl Schleicher s 200 Streifen fltrier Papier Nr 2043 Bb ETAM Veronal buffer Grassman Cow
5. y BOSLUWBISHORARAICHASL LAS ARF OO 25M PE BA HHH Mic A Supernatant WEE AEH Ait Comparison of protein contts of saline and water extractsof ox liver 7y BP7VUYORK SS Fig 4 EOS d 0 RR Ta Supernatant hyaloplasma y
6. 25 1 5 28 30 10 Buffer 0 04cc 8 Rizk RBA ORS COLES UT He eT 100 C10 COR B P B Brom Phenol Blue 0 05 AK 1 04 Wie 2 adest 100 CT 5 207M RAL 0 5 2 0 DRIER RCL TERA 2 DA 10 220 EREA 0 25M
7. V ik FAR MERO RAE KE Plum activate 882
8. W C Schneider1 11 191 0 25M 9 10cc type 40P 600g10 HSK 8500 g 10 0 25M 25cc 8500 g 10 2 5cc 18000g 60 0 25M 2 5cc 18000g 60 2 5cc
9. Fig 1 Effects of the fractions of mitochondria microsom supernatant of G P liver on the maturation of reticulocyt i x AEL Fig1 2 Fig 2 Effect of fractions of mitochondria microsome and supernatant of ox liver on the mturation of reticulocytes Fig 3 Effect of protein fractions of the SuDernant from sucrose extract of ox liver on the maturaton of reticulocytes RATE MER DOA AIC BSS SFE 881 Fig 4 Protein contents of the supernatant of sucrose extract of ox liver
10. extract revealed that it contained a fraction of y globulin which was found to be lacking in the water extract Thus the ripening substance of reticulocyte has been prove to be a protein belonging to the y globulin fraction originated from the hyaloplasm
11. 4 278 1942 7 2 8 40P fe OR RK eR 3 y lt 4 5 y RAPER WETS RRA ARR RES CHH 480 9 10 W C Schneider J B C 259 176 176 11 W C Schne
12. 616 155 156 et x W fe E 33 11 24 i 1 m KEP Plum DO 2 3 pH5 4 HOE
13. ider G H Hogeboom J B C 123 183 1950 12 G H Hogeboom W C Schneider G E Pallade J B C 172 619 1948 13 G H Hogeboom W C Schneider G E Pallade Proc Society exptel Biol Medi 65 2 320 321 1947 14 883 Studies on Reticulocyte Ripening Subsuances Part 3 The cellulor Origin and the characterisbics of the Ripening Substance By Hiroshi SANADA Department of Pathology Okayama University Medical School Okayama Japan Director Prof Satimaru SENO From the results presented in Part 2 it is supposed that the reticulocyte ripening Substances in the bovine liver found by the author may be protein belonging to a globulin fraction or a substance attached to some particles of the cytoplasm which can not be precipitated by the general centrifugation The supernatant obtained from the saline extract by centrifuging at a low speed was again put to ultra centrigu gation at 40 000 r p m thus splitting fraction into two soluble fractions and microsome or mitochondrial fractions Ripening test on reticulocytes proved that the effective substance had been transfered to the soluble fraction The globulin fraction obtained from the saline exfract by the half saturation with ammonium sulfate also proved to be effective Electro paper chromatography of the protein obtained from the saline
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