E.coli HST16CR Competent Cells
Contents
1. If4R4 Tel 077 543 6116 Fax 077 543 197 http wWwW taKara DiO CO jp v201210Da2. 9 cm 100 ul http www takara bio co jp 2 9131 1 2 14 ml BD Code 352059 352057 1 5 ml 3 100 ul DNA 10 ng DNA 4 1 5 ml 42 60 5 SOC L broth w b broth
3. DNA mm mcr8C hsdRMS mcrA DNA pUC o X Gal X Gal 5 Bromo 4 Chloro 3 Indolyl B D Galactoside I 1 Eco7HST16CR Competent Cells 2 100 ul 14 ml 3 DNA 10 ng 4 30 5 42 45 6 1 2 7 37 C SOC 1 ml 8 37 1 160 225 rpm 9 10 37
4. L broth 10 g Bacto tryptone 5 g Bacto yeast extract 5 g NaCl 1 L water pH 1NNaOH 75 b broth 20 g Bacto tryptone 5 g Bacto yeast extract 5 g MgS04 7H2O 1L water pH 1NKOH 75 6 7 7 L 10gBactotryptone 5 g Bacto yeast extract 5 g NaCl 1 L water pH 1NNaOH 7 5 1 5 agar 8 X Gal 20 mg ml X Gal 100 ml 200 300 ul 9 80 1 IV 1
5. 31 IdfaR4 E coli HST16CR Competent Cells vV201210Da E coli HST16CR Competent Cells 100 ul Xx 10 pUC19 plasmid 0 1 ng ul 10 ul SOC 1ml x 10 SOC 2 Tryptone 0 5 Yeast extract 10mM NaCl 2 5mM KC 10mM MgSO4 10mM MgCl2 20mM Glucose Ill DNA Hanahan E co7HST16CR Competent Cells co 7HST08 Premium Competent Cells ColE1 ori pcn pc pUC E coli HST08 Premium Competent Cells
6. DNA Ligation Kit Mighty Mix gt 6023 TaKaRa DNA Ligation Kit LONG 6024 E coli HST08 Premium Competent Cells 9128 co7 HST08 Premium Electro Cells 9028 pUC118 DNA 3318 pUC119 DNA 3319 BAP DUC118 DNA 3320 3324 X Gal 5 Bromo 4 Chloro 3 Indoly 8 D Galactoside 9031 NucleoSpin Plasmid 740588 10 50 250 NucleoBond Xtra Midi 740410 10 50 100 NucleoSpin Plasmid NucleoBond Xtra Midi MACHEREY NAGEL
7. 1 ng pUC19 Amp gt 1 Xx 108 colonies u g pUC19 2 8B o PUC19 100 ug ml 60 ug ml X Gal L V Genotype co7 HST16 CR F endA1 supE44 thi 1 7ec4 7 e 4 7 gyrA96 phoA 80d lacZ A M15 A laczYA argF U169 A mrr hsdRMS mcrBC AmcrA ApcnB A http www takara bio co jp 3 9131 VI Cell density 1 2 X 103 bacteria ml VII 80 80 pUC19 VII 1 Hanahan D 1983 Mol Biol 166 557 2 MessingJ 1985 Gene 33 103 3 Lopilato J et g 1986 Mol Gen Genet 205 2 285 90 IX
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