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Contents
1. EIA EIA EIA ELISA2. 18 lt 23C 1 5 3 9 B 0
3. 4 500ng ml 10 8 PBS 3 50 B 5 0 B 50 B 100 3 1 2 1 2 000
4. LISA B 1 SD 1 U S A i 12
5. 15 lt 30 2 40 60 20 450nm 1
6. 3 ELISA A
7. 8 6 D ELISA 1 39 2 61 66 1998 2 1998 10 736 747 1998 1997 3 Yalow R S Beeson S A gu7e 184 1 648 6 Meulenberg E P Mulder W H Stoks P G 1959 Environ Sc1 Technol 29 553 1995 4 Krotzky A J Bernd Z Pure az Appl 7 20 Chenm 67 2065 2088 1995 1997
8. KW GS i 1mg ml 1 5 20ng ml B 0 09 0 17 3 2 2
9. 1g ml B 4 50 B 16 500ng ml 5 50 B 3 800ng ml 3 6 5
10. 30 30 B B x 100 5 0 5 1 0 g ml B 50 B 6
11. 5 1997 1 42 50
12. x8 i Enzyme Conjugate Negative Contro1l iv Substrate v Chromogen vi vi Stop Solution 4 8 C 2 PBS NaH2P04 2H20 9 0g NaHP04 12H20 64 5g NAC ei en 160 0g 20L 3 Model1 6 4
13. 10g 1 g C 1 i 1 30 100 500pg ml 30 100pg ml 2 R 0 95 B 100 ppb 17 2 n 8
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