“WAKO BIO WINDOW” No.64(2004.12)
Contents
1. UBP296 0000 ada MD Native GLUks Native AMPA Recombinant Recombinant Recombinant apparent K ICso GLUxs ICso GLUxes IC5o GLUxs GLU ICso UBP 296 97 6 300 300 000 0000000 U n 00000000 559 64201 10mg 38 400 BP 2078 U 296 50mg 166 100 00000000000000000 000 0000000 U 00000000 536 51281 069 Domoic acid 1mg 27 100 000000000000000000000000 5mg 110 300 525 43881 0 SH 0 5 lodowillarcdiine 10mg 31 400 000000000 0000000000000000 000 50mg 141 800 533 81743 0222 Kainic acid 10mg 34 100 537 81741 00000 000000000000000 50mg 143 400 530 51201 2081 10mg 31 400 536 51208 00000000000000000000 50mg 130 300 mH UBP 10mg 35600 ue 0 0 00000000000000000 9 UDUDDUDDUDAMPAJDDDUIDDDDDDDDDDDDUDDDDD 50mg 155 300 JT UBP 301 10mg 35 600 1766 RH 0000000000000000 09 50mg 155 300 KainateReceptorTocrissetrM _ 1824 15 m 0000000000000000 si 0000 0 U S Wako Bio Window No 64 DEC 2004 7 02000000000000 1000000000000 DOJINDO MOLECU AR TECHNOLOGIES INC Biotin Labeling Kit NH2 0 000000000000000000000000000000000000000000000 0 00000000000000000000000000000000000000000000 200 9 1000310000000000000000000000000000000000000009100 0 00002. 00000 0 0000000000000000000000 00 euunnnn 00000000000000000000000000 000000000000000000000000000 0000000000 000000000000000000000000000 000000000000000000000000000 ses 0000000000000000000000000000 0 10 55 00000000000000000 000000 00000000000 0000000000000000000000000 0000000000000000000000 e 0000000000 000000000 0000000000 00000000000 00000000000 00000000000 00000000 220000 02506000 207 Uu 000 0 0 0000000000 00000000 GPCR IH DO DD UD U 00000000000000 Index 5 gt L Control 33nM 67nM Treatment 100nM 150nM 230nM 340nM 500nM 0 20 40 60 80 100 Time hour Fig 1 Pacitaxel1 0000000 0 351 000 H460 l2 Bio Window No 64 DEC 2004 medium X ACEA 0 00000000000000000000000000000000000000000000 00000000000000000000000000000000000000000000000000 96x0 000 16X0 000 000000 207 cell 00000000000000000 000000000000000 0000000 elo 0 00000000000000 of Apoptosis Viability 120 100 4 80 4 60 4 40 20 4 e Apoptosis detected ssDNA Assay s Viability Measured on RT CES 0 23 46 93 187 375 750 1500 Staurosporine nM 29 20 sDNADO00000000000 0000
3. 12 00000 0 1 gt D U IL TMBZ 3 3 5 5 tetremethylbenzidine O5mx 1 du MERIT He 20mx 1 gt 0 10 00 PBS Tween 20 400ml 40mx 1 PD EL IL 0 a a 15m x 1 uU uult 0000000 0000000 000000 0 JC V 000000 0 e C V 9 1634 06 0 786 1 8 n 5 1594 19 B ri 9 0 692 29 68 n 5 1464 10 5 0 555 14 15 9 1261 16 0 000000 0 e jc v 250 ri 5 0 965 18 A 5 378 32 500 ri 9 0668 18 B ri 5 649 47 1000 ri 5 0449 13 5 665 24 2000 nj 9 0 278 25 OOD 0 2100000000000 1000000 0000000 0000000 ng mf 000000 U UIC V 0 000000 0 e C V D rii 15 1 540 4 6 N n 15 0 766 4 2 ri 15 1 478 6 1 B r 15 0 673 34 63 ri 15 1375 49 n 15 0 538 28 125 nj 15 1194 44 000000 0 C V 250 15 0 931 37 ni 15 375 67 500 15 0 648 29 B nj 15 470 45 1000 ri 15 0431 37 15 675 33 2000 ri 15 0272 26 CMJ O DD Bo 2100000000000 1000000 JO Wako Bio Window No 64 DEC 2004 000000 000000000 20 r 2 0 Dn 15 154 000 es k Il 1 0 10 e 05
4. Uu 0 0 0 0 000 RNase 000000 0 0000000000000000000000 00000000000 00000000000 00000000000 000000 00000 RNase 00 0 0000000 00000000000 L 00 RNA 0 0 0 gt 3 5m 0 0000 Ux 10 00000 0000000000000 0 Invitek Invisorb Spin Tissue RNA Mini 00000 3791 0000000000000000 0 O 0 Wash Buffer R1500 0 0 000000000 504 0 0 0 3 4 1 2 1 2 3 4 lane 2 285 185 lane 3 lane 4 00000 Total RNA 00000 Total RNA 0 lane 1 80 37 37 Wako 00000 RNase 00000000 RNAg 000000000000000 0 0 0 RNA 00000000000 Total RNA 0 0 oO O 1 L3 r3 DL r3 BC pr B mn 2 2 L3 r3 DL p BC pr HB mn L3 C3 r3 C3 r3 C3 r3 000 00000 0000000 0000000000000000 RNA 000000000000000000 000000000000 000 OU x 1 100 00 00000000000000000000 000 180 01891 UUUUU
5. 000 575 76611 000000050 00000000000000000000 0 000000000000000000000 0 0000000000 ULTRA 000000000000000000000000000 00000000000000000000000000000000000000000000000 0000000000000 0000000000000 0000000000 u 0 d dan ama 7 500 000 572 76621 000000050 8500000 579 76631 00000000 9 250 000 576 76641 00000000 9 750 000 Wako Bio Window No 64 DEC 2004 17 000000 1001 C 1000 KIKKOIT arn ENN 00000000000000000000000000 Ao0000000000000000000 0000000000000000 0 DO0000000000000000000000000000 0 c 1000 000000000000000 00000000000000000000000000000000000 0 i 1
6. 0 LIL LIL 0000 0000 0000 0000 0 0000000 0000000 000200000 00000000 1 1 LIL ON 1 0 RNAstebilizer RNase Irreversible Inactivation Reagent Invitek 000 00000000 RNAI 00 0000000000 00000000000 00 0000 0000000 0000000 0 0000000 0 00000000000000000000000000000000000000 0 I I 0000000000000 0 0 000000000000000000000 00000000 00000000000000 RNA 0000000000 000000 o ag 0000000 12 000 000 No 00000000 500 99923 508 99924 22 Wako Bio Window Invisorb Spin Tissue RNA Mini Kit No 64 DEC 2004 501 0 2501 0 uuununnununnpNAn 0 O DNA Extractor SP Kit DNA Extractor SP Kit 0 0 I Serum D 0 0 0 0 0 0 0 0000000000000 00000000000000000000 0 00000000000000000000000 0 000000000000000000000090000000000000000000000 0 0000000000000000000000 0000000000000000 224 100000000000000000000000000000000000000000 DNA 00 00000000 2 000000000000000 00 0000000 000000 00000
7. U 0 00000 URL http 0 00 00000 00000 CDI 000 Wako Bio Window E mail biowin wako chem co jp F A X 06 6201 5964 JOON D M 00000000 000 04260 000 UU G K ege e e ede ego e e e e ege ege e ege e e Fe Fe e e oe ege e e ege ege ege ege te e oe e e ee e oe ege ege ege ege ege ego Fe L LT LT L U qu D 000000000 000000 00000000 00000000 000000000 0000000 0000000000000000 Wako Bio Window No 64 DEC 2004 15 00000000000000000000000 00000000000 TECAN 000002 000000000000000000000000000000000000000000000000 000000000 200000000000000000000000000000000000000000 00000000000000000000000000000000000000000000000000 0000000000 00 enununnnnn enununnnunnnn 0000000000000000000000 00000000000 0000000000000000000000 000000000000000000000 Epid 3n 0 0 0 0 50 des 000000 TIT 0000000 000000 gt 001000000000000000 gt 00 0 Ex 230 850nm Em 280 850nm gt 000000000 p 0000 5 20n 280nm 0 0 0000 251 100 T gt 0 410000000 gt 0000000 470 530 590 635nm Em 280 850nm gt O00000000 gt O000 0230 1000m PP gt 0 11 410000000 98 000 1538 000 00000 eee 222000 0000000000000000 0 3500000000 00
8. 0 0000 000 0000 620 0000 000000000 1921901 SuperSep 7 5 D2well 5 40 000 200 000 10 12 000 00000000 199 12911 SuperSep 7 9 17well 100 2 000 10 12000 196 12921 _SuperSep HMM 20 000 130000 10 12 000 193 12931 _SuperSep 10 I7well 50 500 10 12 000 190 12941 SuperSep 12 5 12well 5i 14 000 80 000 10 12 000 19712951 SuperSep 12 5 17well 30 300 10 12000 194 13061 SuperSep 15 2well 5 6 000 60 000 10 18000 19113071 SuperSep 15 17well 20 300 10 18000 194 12961 SuperSep 5 20 12well T 10 000 200 000 10 12 000 19 SuperSep 5 20 17well 50 750 10 12000 198 12981 10 20 I2well _ 10 000 130000 10 12 000 195 1299 SuperSep 10 20 17well 50 500 10 12 000 _ 1 000 80 000 190 13301 SuperSep 12 5 2D 7 30 300 10 18000 10 000 200000 19713291 SuperSep 5 20 2D 5m 7501 10 18000 000000000000000000 190 13421 00000000 0000000 300 00000 0000000000000000 TOTTI 2202221 WIDEMEW Prestine Protein Size Marker 11520304050 791p 5094 18000 000000000000000000000000000 23302271 WIDE VIEW v
9. 0000000000000 0000 2509 5 800 035 15801 D00000 000000000 0000 2509 8 800 034 13291 00000000 25mg 19 900 038 10531 100mg 3 700 034 10533 00000 19 22 000 032 10534 5g 78 000 031 17601 100mg 5 000 037 17603 0000000001 OOO O 500mg 16 000 085 17604 19 26 500 038 17851 100mg 4 500 034 17853 000000000 V 00000 500mg 15 800 082 17854 1g 28 600 085 17861 100mg 5 000 031 17863 000000000 X 500mg 18 000 039 17864 19 32 000 00000000000000000000000000000000000000000000000000000000000000 000000000000000000000000000 T SAKIN SS AL 540 8605 0000000000001020 amp 06 6203 1788 0000 T 103 0023 5 130 0303270 8243 0000 00000 3 092 622 1005 00 ell 08285 6380 0 0 00000 0520772 0788 00 el 04576 2060 00000 3 02958 227400 ell ONU 802272223072 0 0 01171 0289 0120 052 099 O 0120 052 806 0 00000500000 DMO I 0000000 E mail biowin wako chem co jp0 0 URL http www wako chem co jp 2 i 01
10. 296 60501 Extractor SP Kit 5010 25 000 1 5 Wako Bio Window No 64 DEC 2004 23 00000 CO O E e nnonm WE uuBudmugt 0000 e 0000000000000000000000000000000000000000000000000 L L 00000000000000000000000000000000000000000000000000 00000000000000000000000000000000000000000000000000 e 1 1 1 2100000000000 e 000000000000000000000600 00000000000000000000000000000000000000100000000000 Jg 00000000000000000000000000000000000000000000000000 000000000000000000000000000000000000000000000000000 E 000000000000000000000000 s 0000000000000000000000000000000000000000000000000 EX 0000000000000000000000000000000000000000000000000000 Hi 00000000000000000 00000000000 00000000000000000000000000 00000000000000000000000 00000000000000000 000 uU D 0 B 1000000000 031 19443 200mg 6 500 7085 19241 0000000001000000 0000 39 2600 26 000 000000 000 fH E 0 00000000 034 11231 10000000 0000000 0000 10019 21 000 033 13901 000 00000 000000000 0000 5m 8 800 03 gt 15791
11. 00000 0000000000000000000 00000000000000000 000000000 9000000000 000000000000000000 00000000000000000 00000000 000000 00000000 0000000 0000000 UUUUUUUU 000 0 00 lt 10m p53 Exon 9 0 0 100000000 saa 2500 0000000000000 1910000100000 PUDUDDUDUDUDD eum nnn Jun 00 10000 0 DNAG O O O 20u 0 TE pH 800 0 FHUDUBDD 30m OOO DNA DODO 54 0 00000000 p53 Exons D DA 50m 0 0 000000000000000000000 pUDUDB 50m 0 0000000000000 DNAD O00 A0 000000000 001 002 003 004 00 005 006 007 008 009000 M A w A w A w DNA Step Ladder Mix 80brf 10kbp DNAT 0000000 SP 1 Aj DNA 000 PCR 401000 3 Agarose gel DNA Extractor SP Kiti 000000 Y Ishizawa M Kobayashi Y Miyamura T and Matsuura S Nucleic Acids Res 22 17741994 2 Sozzi G Musso K Ratcliffe C Goldstraw P Pierotti M A and Pastorino U Clin Cancer Res 5 2689 1999 3 Silva J M Dominguez G Garcia J M Gonzalez R Villanueva M J Navarro F Provencio M San Martin S Espana P and Bonilla F Cancer Res 59 3251 1999 4 Shao Z M Wu J Shen Z Z and Nguyen M Clin Cancer Res 7 2222 2001 0000000000 000 000000 U J 100000000
12. CONTENTS HG D0000 v 2 000000000 2 40 eee 2 000000000 2 0 x4p e p 2 0000 0 50500000 0 00000 vvv p 4 J ena Bioscience Mant 0 0 0 0000000 us p 5 0 00000 v 6 00000 000 1 DDUDDD vM M M M p 24 5 DMT Biotin Labeling KiENH2 p 8 RSD 0000000000 6 p 9 000000 9 0000000 emm 10 000000 Anti c Kit Monoclonal Antibody lt p 11 298 7 Du 0000000 ECOS Competent E coli 5 J M109 p 20 00000000000 5 ee 21 000000600 BB n 22 DNA Extractor SP p 23 PIERCE Slide A Lyzer Dialysis Cassettes 12 30ml p 4 ACEA RT CES DDDDDDU D OO 00000 p 12 NOVELTEC 000 eme MM p 14 00000 16 0000000 17 000000 000000 C 100N C 1000 18 000000 0000000000000 e 19 uu 000000 e M p 13 asas noa p 15 O Wako SuperSep U ll 0000000000000000000000000 0000000 00000000000000 00000000000000000 00000000000000000000000000000000 0000 0000000000000 000000000000000000000000000 0000000
13. 2004 5 000000000000 0000 Tag 00000 0 00000000 00000000 0000000000000000000000000000 0 50 000000000 000 000000000000000000 0 PAED 0 HA I 0 00000 MMS JOIPT HA 11 0 0 00000 5 19 1 0000000 0000000000000 Photo courtesy of Alexander Sorkin at UCHSC 0 Photo courtesy of J Brown amp I Davis UCSF 00000000 amp 000000000000000 000 000000 U 0 00000000 6His 583 78891 BIOT 159 100 Anti 6 His Monoclonal Antibody Biotin Labeled 0 1m 68 400 586 78901 159 100 Anti 6 His Monoclonal Antibody FITC Labeled 0 1m 68 400 583 78911 MMS 156P 500 Anti 6 His Monoclonal Antibody Purified 0 5m 86 600 9E10 582 78861 150 100 Anti 9E1D Monoclonal Antibody Biotin Labeled 0 1m 68 400 589 78871 FITC 150L 100 Anti 9 10 c nyc Monoclonal Antibody FITC Labeled olm 68 400 586 78881 MMS 150 500 Anti 9E 10 c myc Monoclonal Antibody Purified 0 5m 86 600 AU1 580 78801 BIOT 130L 100 Anti AU1 Monoclonal Antibody Biotin Labeled 0 1m 68 400 587 78811 FITC 130 100 Anti AU1 Monoclonal Antibody FITC Labeled 0 1m 68 400 584 78821 MMS 130P 500 Anti AU1 Monoclonal Antibody Purified 0 5m 86 600 AU5 581 78831 BIOT 135L 100 Anti AU5 Mon
14. 0000 000 U D 0 D 00000000 301 09401 000000 PD 10 1 198 000 308 0911 0 000000 PD 10 1 30 000 000000 10 0000 0 000 0000000 0000 25 000 18 Wako Bio Window No 64 DEC 2004 ATP 10 100 OOOO L 0000000000000 000000 2591000000000 ATPD O O 000 100 UU 0000000000000 30000000 0000000 251 0000000000000 10 90 ATP 00000 000000000000000000000000000000000000 0000000000000 10 00000 1 1 1 10 10 00 00 RLUD 10 10 10 10 0000 RLUD 10 10 10 107 M 10 10 10 10 10 10 10 10 10 10 0 00 CFU m 107 D D 000000000000 000000000 000000 00000000 150 Hmm B 00 00 1 00000000000000 55000 AR n nn d 00000000000000000000 ET 000000000000000000 00000
15. 54 000 557 64861 1845 SH 025 Recombinant Human Sonic Hedgehog C2411 N Terminus 230g 60 000 554 64871 AF1568 Mouse Hip Affinity Purified Polyclonal Ab 10019 73 000 551 64881 AF196 Mouse Desert Hedgehog C Term Dhh C Affinity Purified 10019 73 000 554 61071 445 Mouse Sonic Hedgehog C Term Affinity Purified 1009193 73 000 551 61081 AF464 Mouse Sonic Hedgehog N Term Shh N Affinity Purified 10019 73 000 558 64891 AF733 Mouse Desert Hedgehog N Term Dhh N Affinity Purified 10019 73 000 551 64901 1568 Mouse Hip Biotinylated Affinity Purified 5019 78 000 558 64911 BAF 196 Mouse Desert Hedgehog C Term Biotin Affinity Purified 5019 78 000 555 64921 BAF445 Mouse Sonic Hedgehog C Term Shh C Biotin Affinity Pur 5019 78 000 552 64931 BAF733 Mouse Desert Hedgehog N Term Biotin Affinity Purified 5019 78 000 559 64941 BAM4642 Mouse Sonic Hedgehog N Term Biotinylated MAb Clone 171028 5019 78 000 550 64971 1568 Mouse Hip MAb Clone 217413 50019 60 000 557 64981 MAB1705 Mouse Indian Hedgehog Ihh MAb Clone 187515 50019 60 000 554 64991 MAB196 Mouse Desert Hedgehog C Term Dhh C MAb Clone 148644 50019 60 000 551 65001 MAB445 Mouse Sonic Hedgehog C Term Shh C MAb Clone 55626 50019 60 000 558 65011 MAB464 Mouse Sonic Hedgehog N Term Shh N MAb Clone 171001 50049 60 000 555 65021 MAB4641 Humary Mouse Sonic Hedgehog N Term Shh N MAb Clone 171018 5
16. Westem SizeMarker 25011 20 000 000000000000000 134 14501 Molecular Weight Marker High Range 7345 79116200 12 800 _ 000000000000000 13 14511 Molecular Weight Marker Middle Range y H 77734 20 30 42 79K Img 12 800 505 00000000000 Laemmli 19611022 Sample Buffer Solution ME 2 55 2 25m 3 100 0000000000 0 0 Laemmig 193 11082 Sample Buffer Solution x 21 x21 25m 3 100 000 000 191 13272 Sample Buffer Solution A co C TID 4 25m 7 600 19813282 Sample Buffer Solutio 2 x 4 B Laemml lt y 6800 184 01291 Running Buffer Solutiori 10 SDS Laemmli 000 0000 00000 1 5 200 299 Quick CBB 0000000 250 2 9 000 174 000000000 300000 250 420 178 00551 Quick CBB PLUS CBB G 2501 0 000 299 13841 Silver Stain Kit wako 0000 101 9 000 291 50301 Silver Stain Il Kitwako 00000000000000000 101 9 000 000000000000000 299 58901 Silver Stain MS Kit 1000000006000 20 0 19000 10100000000000 293 57701 Negative Gel Stain MS Kit 1000000000000 20 0 1 000 Wako Bio Window No 64 DEC 2004 3 0000000000 00000 5 1000000000000 0000000000000 998 000000000000000 00000000000000000000000000 L3 0 0 0 r3 Bono L3 Cj GAG C Lr r3 000000000 00 0000000
17. 0 0 0 0 B 100000000 58785021 000002 1 8 875 000 583 84381 O00002 1 9 625 000 580 84391 O00002 1 12 962 500 583 84401 000002 FAL 96 4 1 13 585 000 585 85061 2 FAL 384 4 1 13 585 000 584 85031 00002 FL 96 1 1 9 865 000 581 85041 2 FL 96 4 1 12 835 000 588 85051 00002 FL 384 4 1 12 835 000 2000000000000000000000 00000000 4100000 PIIOUUUUU 1100000 96810000 3843 000000000 0000 M K lO Wako Bio Window No 64 DEC 2004 0000000000000000000000000000 0 0 UU 00 v Lj 0000 L3 r3 Lr 1 KEE 825 L3 C Lr Lr LILI C C r r r r m 0 0 v Y w YV E BE E O EE FS G o rrr m o a0 m o a0 000000 00000005 00000003 00000000 2300 900nm 52 0 5fmol well 230 1000nm 0 5 0 0 0 00500 492nn1 400 700nm 0000 34000000000 D 384well plate Fluorescein 10pNI 1fmol well 384well plate 00000000000000 00000000000000000000000000 soosoo 00000020 1 5 350 JI JII L 0000000000000 eq aac uuu 0000 0 0 514mm 545 280mm HO 38 5kg 0000 TRF E 00000 0000000 00000003
18. 0 5 0 0 1 1 j 1 0 0 L 0 3 63 325 250 500 100 200 0 3 63 425 750 500 4000 000 0000000 ny 00000 000 0000000 0 Uu gH 1000000000 304 15501 PCJI3 00000 0000000 96 0 96 000 00000000000 7000 000 0000000 Uu Uu H 1000000000 304 04631 PRAO 00000000 96 0 96 000 301046431 PRBO l 000000000 96 0 104000 308 04651 PRCO31 000000000 96 0 96 000 5 661 PRDO5l 00000000 96 0 96 000 302 04671 61 0004 00000 96 0 96 000 305 07101 PRI121 0000 CINC 1 96 80 000 306 04691 PRGO9L 0000000000 981 96 000 309 04701 111 0000 _0008 gt 000000000 96 0 96 000 ICE Profa 68 813 Anti c Kit Monoclonal Antibody 00000000000000000000000000000000000000000000000000 000000000000000 00000000000000000000 0000000000000 0000 Gastroenterology 1999 117 140 148 DUUNo Dp Umm 1000000000 303 15571 MIOLIA Anti c Kit Monoclonal Antibody 0 5mg 20 000 G T Wako Bio Window No 64 DEC 2004 ll UBHHHZUBSGUSSHSHEDBSBHSSUSHHBEHSBHU RT CESPTTETLDODDD O UO D 00000 5 00 5
19. 00 s Targeted siRNA _ Scrambb siRNA 0 8 4 0 6 4 Cell Index N Cell Index 0 4 4 0 2 4 Poly L Lysine a Fibronectin 2 0 3 0 4 0 Time hour D Fig 4 Poly L Lysine Fibronectin 0 0 0 0 0 000000000000000 31D D D 313 00 0 0 10 20 30 40 50 60 70 80 0 0 1 0 Time hour U Fig 3 sRNA 00000000000000 5100000000000 1 5 EGF 1 25 4 4 EGF Treatment B A 0 75 4 Cell Index 0 5 4 0 25 4 30 40 Time hour 0 29 50 0000000000 31000 Cos 7 00 000 0000 u 0 000000000 581 84821 1GX J 16900 x6 100 11 500 000 588 84831 9 X J 96100 Xin 12 000 000 583 84881 516 161000000 HUD 6 000 55 000 580 84891 RT CES96X 96100 0000 iis uuu 120 000 M Y 0000 2005 000000000000000 0000 0 000 Tag ein 10 0 000000000 Wako Bio Window E mail biowinoweko chem co jp F A X 06 6201 5964 COVANCE Wako Bio Window No 64 DEC 2004 13 0000000000000 GAIT Gait Analysing Instrument and Software for Test Animals 0000000000
20. 0000 0000 0 00000000000000 0 00000000000000000000000000 000000000 1180 Cum 18 HEE k 000 0000000 u 0 100000000 566 57541 14 603 1108 p8 amp active 10490 68 000 562 57543 14 604 PI3Kinase p1165 p8 active 10490 68 000 563 57551 14 558 P1I3Kinese p120y 10490 64 000 000000 000 0000000 u 0 u 0 100000000 566 51441 07 016 Anti PTEN 1001 60 000 560 56841 14 488 active Sg 64 000 U K 0000000000 D D JENA BIOSCIENCE Mant 0000000000 Mant 0 N Methy anthrenloy Ti 0000000000000000000000000000000 00000000000 00000000000 000000000 000000000000000 000000000000000000000000 NH2 ids N o o o gt N PS MM CX 3 P CX X HO O N N HO 0 09 o N N NH L d 50 50 50 9 cO 20 e NI 2 CH3CH2 NH d 3 CH CH NH 2 CH4CH NH ab MT E gt a E CHs NU 201S NU 202S NU 204S 000 0000000 u i u D 00000000 579 98091 2015 mant ADP S pack 150Units 25 500 572 98101 2025 mant ATP S pack 150U nits 25 500 576 98121 NU 204S mant GDP S pack 150U nits 25 500 1 Unit 1u of a 10 solution Jena Bioscience GmbH 0 0 00000000000000000000 0000 PCRJ 0000000000000 0000000000000000000000000000000000000 3591 00000000000 U F Wako Bio Window No 64 DEC
21. 0000 00000000 562400 00000000000000000000 000000 reas RLUI 000000 0000000000 000000 150 200000 00000000000 00000000000000000000 6 70 SI 90 0000000 0 RLU O RLU O O RLUI 1 12119 4101 00 27910 173750 0 WTO cloacae IAM12349 231 307 0 17370 4484 0 pneumoniae NISL4308 210 235 609 1 3992 C freundii JCM1657 297 253 423 0 14630 000 U D 00000000 302 09291 00000 250 259 0 31 000 300 09231 000000 2501 250 37 500 307 0222 000000 HALO 1001 0 40 000 304 09251 000000 ATP D 2501 0 25 000 309 09321 000000 100 1001 0 45000 301 09261 000000 000 201 5000 301 13431 00000 150 1507 0 40 000 300 09351 35m0 1000 12 000 00000 259 0 0 0000000000000 0 000 1 H D 00000000 303 09221 000000 91000000000 0000 1001 0 27 000 305 09281 0 00000m 000000 PD 300 000000000 1001 0 27 000 303 09341 000000 1001 0 38000 G K Wako Bio Window No 64 DEC 2004 19 000000000000000000000000 ECOS Competent E coli DH5a J M109 0000000 DHS 0 109 0 0000000 00000000000000000000000000000000000000000000000000 0000000000000000000000 15 25 000000000000000000000000 00000000000000000000000000000000000000000000000000 000000000
22. 000000 1 1 1 1 1 1 e 00o L1 1 1 1 000 uur 584 84573 20765 4 000 582 84574 20765 Dodecylsulfate Nar salt in pellets 7 000 588 81576 20765 1kg 17 000 U S 39039039 ege ege ege ege ege ege oe ege oe ee oe oe oe oe ege oe oe oe oe oe oe oe oe oe oe oe oe oe oe oe oe oe oe oe oe oe oe oe oe e e Slide A Lyzer O PIERCE Slide A Lyzer Dialysis Cassettes 12 30m lt 0000000000000000000 00000 12 00000000000000000000000000000000 eu lun el lll SIN Ul 0000000000000000000000000000000000 000000000000000 582 83751 66830 Slide A Lyzer Dialysis 10000 12 3017 1 6 16600 20000000000 ox 12 000000000000 Wako Bio Window No 60 4 200412 I3 0 0000000 URL http wekochemcoo jp siyaku joumal U K 4 Wako Bio Window No 64 DEC 2004 PISK AK 000000 pstate PIX 00 UU Phosphatidylinositol 3 Kinase 0 O 00000000000 3 0000000000000 PH 3 42 PH 3 4 5 0000000 0 0000000000000000000000
23. 000000 ZU 0000000000000000 0000000000 00000000000 ennnunnnn 00000000000 0000000000 000 0000000 0000000 342 90841 DeoFec GYl 18m 2500 8 Bio Window No 64 DEC 2004 0000 dud 0 0 RS _ RD UO mds 0 0000000000000000000000000000000000000 0 000000000000000000000000000000000000000000 1 0 000000000000000000000000000000000000000000 Tri T j 6000000000000 58 0000000 0 0000000000000000000000 U U LU O 551 610811 Antigen Retrieval Shh C0 0500000000000 00 No 554 Reageng 0 Nog 559 650411 000000060 607111 00000000 U 0 0 Tissue Staining Kif 0 1 556 65051 00 00000 0000000 00 No 0000000 0 00000000 553 64841 1568 050 Recombinant Mouse Hip 5019 60 000 550 64851 1705 025 Recombinant Mouse Indian Hedgehog C28I1 N Terminal Peptide zug 60 000 551 63801 461 SH 025 Recombinant Mouse Sonic Hedgehog N Terminus Shh N 2 9 54 000 554 62811 1314 SH 025 Recombinant Human Sonic Hedgehog N Terminus Shh N 231g
24. 000000000000000 HU UL 12345678 91011 12 000000 100 10XW x Timm pu 0 0 0 008 SO Wix T mm i gt O n 00 354 m lowslT 25 well B L 0000000000000 6 000000 j 0000 10mA 20mA amA gt 0000000 135 7 3 2 000000000 HG 5 207 12well 000 No 195 13611 1 000000000 D 000000 CBB IO 000 No 178 00551 00000 Lare1 2 3 7 11 1208000000 00000 Lane5 90 000000000 000 No rd n d 00000000 195 1360 supersep HG 5 20 12well 10000 200000 10 15 000 GEP 192 1391 SuperSep HG 5 20 17well 50 750 10 15 000 5 199 13631 SuperSep HG 10 20 12well 10000 130 000 10 15 000 5 196 13641 SuperSep HG 10 20 17well 0 50 500 10 15 000 2 K TA SDS PAGE T D DB D LI D D D D 000000 2MERIX 40 D D 00 2 Mercoptoethenoll 0 0 SDS PAGE 0 4J 0 00 00000000 0000 025mol 6 8 0 8w SDS 40w v Glycerol 0 02w v 20vol 2 Mercaptoethanol DD Dn o o 0000000 1911272 Sample Buffer Solution 2ME 4 00000 7 600 000000000 2 x 40 0 D 00 2 Mercoptoethenoll 0 0 0 SDS PAGE 0 4 00 0000000000 0 25mdl 1 Tris HCI pH68 8w v SDS 40w v Glycerol 002 3 oo fon 00000000 CHD 198 13282 Sample Buffer Solution 2ME 4 00000 6 800 Wako Bio Window No 64 DEC 2004
25. 0000000000000000 2000000000000 19 000000000000 0 00000000000000000000000000000000000000000000000 0 0000000000000000000 G E J E 00000 00 Ul 210000000000000000000 NH gt Reective Biotin 00000000000000000 Filtration Tbe 0 0000000000000000000000000 00000 samples gt NH gt Reactive Biotin 3 Reaction Buffer 0 5m x 1 p WS Buffel 4m x 1 P Filtration Tube 3 ewe 0 0000000000000000 gud 19600000 lt diis f jS 1122 uu 7 10000 DD 000000 000000000 sceo gt T 2 ii ee 000006 Y elis EN NS p 5 nm d 2 AA s HN 5 a N p 2 Reactive Biotin 000000 dep 000 0000000 00000000 pp 347 90891 LKO Biotin Labeling Kit NH2 3samples 12 000 DOANDO DoFect GT1 yn 0000000000000000000000000000000000000000 00000000000000000000000000000000000000000000 00000000000000000000000 5 4 9 00000000000000000000000 0000000000000000000000 00 ul 000000 000000000
26. 00000000000000000000 00 00000000 5 0000000000000000 1x 10 g pUCI9 DNA 5 H 00000000000000000 U Ecos 1 0000000000 00000000000000 00000000 00 q 0 0000000000000000 000000000000000000000000 10000000000 MMU 0000 T uuuusjiunnunuunun 000000 05 000000000 Y 000000000 2131000000000 42 4810000000000 0000000000 000 16 12 379 000000000000 HH TUI 00000000 000 31006231 10Qux 2 9 000 nm 316 0233 ECOS Competent coli 109 1 20 36000 317 0621 10 2 9 000 mn 7313 06043 ECOS Competent coli 109 10 20 3600 20 36 000 000000 00000000 000 GEP 3406251 Beads 1007 0 4 400 Ligation Convenience Kit 10 D 3 200 igation Convenience 100 20000 319 0591 Hi Competence Broth 20 18 000 319 01343 Z Wako Bio Window No 64 DEC 2004 0000000000000 000000 p 000000000 0000000000000 0000000000000000000000000000000 00000 00000000000000000 00 0 PAGE 0 00000000000000000020000000000000000000000 000000000 siRNA 000000000000000000000000 3910000 59000000 00000000000000000000000 D 0 siRNA siRNA 0 21bases 21bases 0 0000000000000001 0
27. 000000000000000000000 000000000000000000000000000000000000000000000000 000000000 00000000000000000000000000000000000000 0 00000000000000000000000000000000000000000000000000 00 UU 0 600000000000000000000 0000000 D SwngTime D Swing Time 00 0000000 Swing Time 00 UU M 0 000000000000000000000000000 0 Ms 0 00000000000000000000000 P 0000000000000 000000 D 00 ss gt O000000000 00 O O gt gt 00000000000 00 gt 00000000000 0000000 0000000000000000000000000000 E 00000 000000000 00000000000000000 00000000000 0000000 2001 392559 00000000000000000 00000000 Swing Time 00000 14 Wako Bio Window No 64 DEC 2004 00000 00000000000000000000 0000000000000000000000000000000000000000000000000 0 000000000000000000000000000000000000000000000000 0000000000000000000000000000000000000000000000000 00000000000000000000000000000000000 1000000000000000000 0000000000000000000000000000000000000000000000000 0000000000000000000000000000000000000000000000000 0000 00000000000000000000000000000000000000000000000000000 000000000000000 00 uu 00000000000 00000000000 25490 000000000000000 nmi t indi miami
28. 0000000000000001 0000020 60 21 20 DJ 3x 3 0 000002 40 01 20 DJ3x2 000000 004 RNase free Water 1 000000 004 RNase free Water 1 0 000 1900 0 0010 900 00000910000000000 d 30000 00 51000 amp 00 E 00000 9 0000000000 m Bu N 0 o8000 ET P E 0000000000 0000000 0000000000000000000000 00000000000 0000000000 0000000000000000000 0000000000000001 2 0 0000000 0 RNAI 0000000000000000 U Excel 3 T IU U UU E mail 0000 B U 0 Accession Nol 0 0 E mail uuu 0000000 20000 00000030000 http 00000000000000000000 BLAST E mail neegtinifty com 00000000 000000 1000000000000 0000 5 101 0 Hea 00000 0000000000 5 10 0 Hal 000000000000000 0000000000000 SsiRNA 000 pGL3 Control 1 gT pRL TK g T PAGED 1001 000000000000000000 00000000000 siRNA 0 0 10Onmol 20000000000000000000000 000000000000000000 2410000 0000000 0000000000000 RNAi 0000000 _ E z r ie NT 5 ri rs diu gru Bin 5 si PAGE 00 Control Control Control 000000 0000 siRNA siRNA 10nmol 1 siRNA 10nmol 0 0000 Wako Bio Window No 64 DEC 2004 21 m Ll 00
29. 0019 60 000 552 65031 MAB733 Mouse Desert Hedgehog N Term Dhh N MAb Clone 126925 50019 60 000 556 64951 DY461 Mouse Sonic Hedgehog Shh DuoSet 1 155 000 553 64961 461 Mouse Sonic Hedgehog Shh DuoSet Economy Pack 45 Plate 1PK 318 000 u uult 00 No 0000000 00000000 559 65041 CTSOI3 Antigen Retrieval Reagent Basic 50m 11 000 556 65051 CTSO08 GoatHRP DAB Cell amp Tissue Staining Kit 50tests 40 000 088 07461 5g 3 800 086 07462 0000000 00000000 Iul 25g 10 500 084 07463 100g 33 500 00000600006000550005 I py7wasmasysemacom U TN Wako Bio Window No 64 DEC 2004 9 00000 90000 00000 0000000 1 T P BH E cI 4 DI 0000000000000000000000000000000000000000000000000 00000000000 0 0 00 0000000000000 0000000000000000000000000000000000000 HI HH 00000000000000000 0000000 310 31 2000ng uU Bui uut PUUUDDUDUUDBUDIDUDBUDDUDUUBDUDUUDUDUHUDUUDUDaQQQQQ QQ 94 x1 gt da gml nsan OO OO O 0 1 10000009000 200mlir sees aaa aaa aa a aa uuu aa aaa a aa 40 1 pyuuuuumnmuuunuuununuunnnnuu
30. 101L 100 Anti 11 Monoclonal Antibody Alexa Fluor 594 Labeled 0 1m 66 100 585 78731 BIOT 101L 100 Anti HA 11 Monoclonal Antibody Biotin Labeled O 1ny 68 400 582 78741 FITC 101L 100 An HA j11 Monoclonal Antibody FITC Labeled O 1nv 68 400 589 78751 MMS 101 500 Anti HA 11 Monoclonal Antibody Purified 0 5m 36 600 IRS 580 78921 MMS 166 500 Anti IRS Monoclonal Antibody Purified 0 5m 36 600 KT3 587 78791 MMS 125 500 Anti Antibody Purified 0 5m 36 600 MBP 589 78991 PSH 131 200 Anti Maltose Binding Protein MBP JPolyclonal Antibody Purified O 2m 72 900 UB DE 000000000000000000 TOCRIS 00 Ul GLUKE GluRS 000000000000000000000 o 0 000000000000000 bHPG 0 0001 melul O 00000000000 0000000000000 0 C 0000000000 991 0000000000 000000000000000000000 00000000000000 H2N uda More er al Characterisation of UBP296 novel potent and selective kainate receptor antagonist Veuropharmacology 47 46 2004 1 gt E 2 Fa
31. 85x 110x 75mm 7009 22 ME aw i 000000000000000000000000000000 M gt 1 1077 1 10 xmol assay O O O O C 30041 O 4 VERLO 107 1x 10 7 Ba 3 a gt 00000000000000000000000000000000 T 10000000000000000000000000000 0000 U U C 100n 00000000000000000000 0000000 0000000000000 1000 80 000000000000000000 00000 000000000000000000000000000 000000000 000000000000000000000000000000 000000000000000000000000000000 00000000 e 0 0000000 0 1000 CH 33D D 000000000000000000 000000 1000 100000 000000000000000 200 1000 100 1000 000000000000000 00 00000 0000000000000 0000 000000000000000 0 4x 101 1x 10 molATP 1x 10 21 1 0 molATP 00000000 00 232 DDD 10RLU 2579 508400 2291 0 000000 000000600 191000 1100 00000000000 00000000 100000 00000000 12 5bmm 000 0000000000 000 0000 0 O O Opa oo 185Wx 110Dx 75Hmm 0 0 000 0000 05900000 700 000 51D 00000 10 509100 38 409000 0000000 100000000 200 p 1009 00 00000 000000 40000 000 U 0 1000000000 307 09361 00000 100 lj 550 000 301 0881 000000 1000 1 760 000 304 09371 0 0 00000 C 100v 1000 1 30 000 300 09851 000000 35m 1 0007 12 000 00
32. oclonal Antibody Biotin Labeled 0 1m 68 400 588 78841 135L 100 Anti AU5 Monoclonal Antibody FITC Labeled 0 1m 68 400 585 78851 MMS 135P 500 Anti AU5 Monoclonal Antibody Purified 0 5m 86 600 CMPK 586 79001 PRB 136C 200 Anti Chicken Muscle Pyruvate Kinas Antibody 0 2m 58 100 Cre Recombinase 587 78931 BIOT 109 100 Anti Cre Recombinase Monoclonal Antibody Biotin Labeled 0 1m 68 400 584 78941 FITC 106L 100 Anti Cre Recombinase Monoclonal Antibody FITC Labeled 0 1m 68 400 581 78951 MMS 106P 500 Anti Cre Recombinase Monoclonal Antibody Purified 0 5m 86 600 GAL4 583 79011 PRB 255C 200 GAL4 Polyclonal Antibody 02m 58 100 GFP 582 78981 MMS 118P 500 Anti Green Fluorescent Proteii GFP Monodonal Antibody Purified 0 5m 86 600 Glu Glu 586 78761 119 100 Anti Glu Glu Monoclonal Antibody Biotin Labeled 68 400 583 78771 119 100 Anti Glu Glu Monodonal Antibody FITC Labeled olm 68 400 580 78781 MMS 115P 500 Anti Glu Glu Monodonal Antibody Purified 0 5m 86 600 O Wako Bio Window No 64 DEC 2004 000 0000000 D D 00000000 GST 588 78961 MMS 112 500 Anti GST Monoclonal Antibody Purified 0 5m 36 600 HA 11 581 78711 A488 101L 100 Anti 11 Monoclonal Antibody Alexa Fluor 488 Labeled 1 66 100 588 78721 A594
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