Home

リアルタイム PCR によるクリプトスポリジウム、ジアルジア

1. 10 11 Q 5 X PrimeScript RT Master Mix O RNase Free dH20 3 15 TaKaRa PCR 1 3 6 20 S DD FH 3 2 1 3 37C 15 85C 5 4C 20 C 7 PCR cD
2. RNA RNase RNA RNase RNase OFF 9037 RNA RNA DN A DNA OFF DNA 9036 2 5 UV 180C 2 Tahaa III 1
3. SAU wNNN VU NNN aun 2 PCR 3 1 PCR 3 5 E DS JF deccm 2 3 1 3
4. PCR PCR Dynabeads GC combo H Dynabeads GC combo eH 196 PET 10 20g 800 ml 1N EDTA Na 30 g IN pH7 4 Tween80 10 g 1 L 100 HAT Dmh
5. PCR PCR PCR PCR PCR PCR L PCR PCR PCR
6. 1 DNA TaKaRa PCR L L1 L1 L1 L1 UV POTALA ye e
7. Tala H a PCR HHHHHHH 2 PTFE 3 Dynabeads GC combo
8. 19 3 30 033006 24 3 2 0302 2 4 l 19 3 30 033006 l 24 3 2 0302 2 4 _ _ 10 L 20 L e g 10 ml 501 100 1 E lt 8 U 50 p 1 Tak R a
9. 10xBufferA1ml i 10 x BufferB1ml 11 TaKaRa PCR 10 ml 3 12 ml 30 1 1 1 2 PET10 ml m 100L 2 PET o gt 1 PET10 ml 2 ml LLL cis 2 IixBufferA1m 9 up _ O 1 5 ml
10. G C 60 75 95 C B PCR FRZ PCR PCR TaKaRa PCR DD 0 2 ml DNase RNase PCR Thermal Cycler Dice Real Time System II 96 Thermal Cycler Dice Real Time System Thermal Cycler Dice Real Time System L e PCR 48 TP900 xu Thermal Cycler Dice Real Time System e TP700
11. 19 3 30 033006 24 3 2 0302 2 4 PCR PCR PCR Thermal Cycler Dice Real Time System ZZ Cycleave RT PCR Cryptospor rd ium 18S rRNA Detection Kit Cycleave RT PCR_6 2 2 188 rRNA Detection Kit I 3 1
12. PCR 25 Tahaa 26 PCR PCR 1 DNA 2 4 8 L L L1 L1 L1 Li PCR DNA PCR PCR PCR
13. 3 1 2 17 TaKaRa PCR L L1 L1 L1 LI 3 Cycleave RT PCR Cryptosporidium 18S rRNA Detection Kit Cycleave RT PCR Giardia 18S rRNA Detection Kit Positive Control Positive Control EASY Dilution 1 2 1 N 2 1 1 X 10 copies 1 Positive Control 2 1 X 10 copies 1 Positive Control 5 1 EASY Dilution 45u 1 3 1 X 10 copies 1 2 1X 10 copies 1 Y bul EASY Dilution 45u 1 4 1X10 copies wl 3 1X10 copies u 18 bul EASY Dilution 45u 1 5 10 copies wl 4 1X10 copies ul X bul EA
14. C BRAM PCR 1 2 lt 10 1 20 1 200 u 1 1000 1 107 1 10 1 1 TaKaRa PCR 1 5 ml
15. PC Cycleave RT PCR Giardia 18S rRNA Detection Kit Giardia Positive Control 23 TaKaRa PCR IV vol FE z dz X N DNA RNA DNA RNA PCR RT PCR DNA RNA PCR RNA DNA RT
16. 50h 50u 10 5 2 RI CUCRUVS 14 Tala H a PCR L L1 L1 L1 L1 6 DNA RNA RNA PCR RNA cDNA PCR 1 e e Positive Contro1 a Q 5 X PrimeScript RT Master Mix 2u1 O RNase Free dH20 6ul AE TAS 2ul 10 1 1 5 1 10 1 RNase Free dH20
17. PCR 1 3 2 12 Ta hd H a PCR L L1 L1 L1 L1 4
18. 2 I rr RR AB Irc 5 1 2 III oo f RR RR RR RR RR 3 f f ron s 10 1 2 3 4 5 6 D PCR 8 IV 24 V RR nn mn A ex ce 30 TaKaRa PCR L L1 L1 L1 LI Tala R a PCR L L1 L1 L1 L1 L 1 P c
19. DOA 55C 30 sec 3 DNA DNA 72 C 1 min kb 4 DNA 1 1 4 1 25 30 DNA DNA d PCR PCR PCR PCR PCR PCR PCR PCR PCR PCR
20. a 5 30 1 6 T 4 0 0196Tween 80 0 1 M 1M TE buffer 10 mM Tris HCl pH7 6 1 mM EDTA 0 zd T T 0 0196Tween 80 B t idi 0 1 M 50u i po Vortex Vortex 10 f 10 1035 fE 0 0196Tween80 mi 0 1 M 500 I Vortex UE 10 10 10
21. 8 0 2 ml 0 2ml 8 strip tube individual Flat Caps NJ600 0 2 ml 8 8 1 5 ml 0 2 ml PCR 1 5 ml 0 2 ml L L1 L1 L1 L1 TaKaRa PCR
22. 2 Cycleave RT PCR Cryptosporidium 18S rRNA Detection Kit Cycleave RT PCR Giardia 18S rRNA Detection Kit RNA DNA PCR RNase HH RNA baT RM 5 RNase H pemenm RNA DNA DNA t q Wn 2 2 T Hh E MM 44 E 3 RNase H 4 14 V ern 0 708m 44r
23. ROX Ct T Y ri TE An T n A rawy Ora prr rPET 1458 7 B Ll H 4 e 1 er was 3 amp 3 my iim Lei t gt ARDI 25 bas Y Uo T E Rmo Lil minat u san 4 5 5 8 G a eth ul ON PETA th 044 Feii D 4 amp 7 3 9 011 02 H 5 a E G T LJ F OX KK ux www G DK E x wx xxLuUxx x xXx xx x X X x P X X X X X Xx H Negative Control kUPositive Control FAM ROX Negative Control Ct Ct OK Positive Control Ct Ct OK TaKaRa PCR A dd gt Pn 00000 Positive Control 10 45 Tr A a s PENZE 0 2 4 8 S8 10 12 14 16 19 20 22 24 28
24. 16 TaKaRa PCR i tidal s p 1 o k Positive Contro1l Negative Contro1 2 X Cycleave Reaction Mixture 12 541 Q Crypto Primer Probe Mix FAM ROX 5 0 1 O RNase Free dli 5 pA Positive Control 2 091 RNase Free dHz0 25 0 1 x 2 X Cycleave Reaction Mixture Giardia Giardia Primer Probe Mix FAM ROX 10 14 2 X Cycleave Reaction Mixture 175 1 OQ Crypto Primer Probe Mix FAM ROX 70 rp 1 O RNase Free dH O 77 ul 23 uld o4 HE o Negative Control RNase Free dH20 2 1
25. 23J 2 Ue Z EUH TaKaRa TEL 077 543 6116 FAX 077 543 1977 TEL 03 3271 8553 FAX 03 32 71 262 TEL 077 565 6969 FAX 077 565 6995 1209
26. PCR Cryptosporidium parvum Giardia lamblia 7 intesti nalis amp t l amp G duodenalis 24 3
27. PCR L L1 L1 L1 LI RT PCR Cycleave RT PCR Cryptosporidium 18S rRNA Detection Kit CY230 O n PE e o RE 25u 1 50 5 X Primescript RT Master Mix 2 X Cycleave Reaction Mixture Crypto Primer Probe Mix FAM ROX 250 1 RNase Free dH 0 Lysis Buffer Proteinase K dH 0 for Dilution Crypto Positive Control EASY Dilution for Real Time PCR 100 1 625 u 1 ml 625ul X 4 50 1 150 1 50 1 1 ml X 2 PCR Cycleave RT PCR Giardia 18S rRNA Detection Kit CY231 10 25u 1 50 Q 1 5 X PrimeScript RT Master Mix 100 1 2 2 X Cycleave Reaction Mixture Giardia 625 1 Q 3 Giardia Primer Probe Mix FAM ROX 250 1 O 4 RNase Free dH O ml um Q 5 Lysis Buffer 625ul X 4 5 6 Proteinase K 50 1 wl T dH O for Dilution 150 1 PCR Q 8 Giardia Positive Control 50 1 Q 9 EASY Dilution for Real Time PCR mlX 2
28. cDNA PCR PCR PCR PCR Polymerase Chain Reaction DNA denaturation step Tannealing step extension step in vitro DNA 1 DNA 10 s 1 3 5 1 4 1 2 1 2 3 25 30 5 3 EM SZ 94 4 DNA g xj j 10 3 4 C n 3 554 4 min 1 PCR DNA 24 TaKaR d PCR DD 1 dNTP DNA AB 94C 30 sec 2
29. Sample 1 1 58 Sample 2 3165 Positive Control Eu 21 TaKaRa PCR Ct Crossing Point 2nd Derivative Maximum 2 Crossing Point CP Positive Contro1 d Ww 23 50 4 45 4 40 TN 35 1 50 4 20 20 15 4 10 4
30. PCR 2 PCR LAMP 1 rRNA rDNA rRNA PCR LAMP e 4

リアルタイム PCR による クリプトスポリジウム、ジアルジア

Contents

リアルタイム PCR によるクリプトスポリジウム、ジアルジア