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リアルタイム PCR によるクリプトスポリジウム、ジアルジア

1. 10 11 Q 5 X PrimeScript RT Master Mix O RNase Free dH20 3 15 TaKaRa PCR 1 3 6 20 S DD FH 3 2 1 3 37C 15 85C 5 4C 20 C 7 PCR cD
2. RNA RNase RNA RNase RNase OFF 9037 RNA RNA DN A DNA OFF DNA 9036 2 5 UV 180C 2 Tahaa III 1
3. SAU wNNN VU NNN aun 2 PCR 3 1 PCR 3 5 E DS JF deccm 2 3 1 3
4. PCR PCR Dynabeads GC combo H Dynabeads GC combo eH 196 PET 10 20g 800 ml 1N EDTA Na 30 g IN pH7 4 Tween80 10 g 1 L 100 HAT Dmh
5. PCR PCR PCR PCR PCR PCR L PCR PCR PCR
6. 1 DNA TaKaRa PCR L L1 L1 L1 L1 UV POTALA ye e
7. Tala H a PCR HHHHHHH 2 PTFE 3 Dynabeads GC combo
8. 19 3 30 033006 24 3 2 0302 2 4 l 19 3 30 033006 l 24 3 2 0302 2 4 _ _ 10 L 20 L e g 10 ml 501 100 1 E lt 8 U 50 p 1 Tak R a
9. 10xBufferA1ml i 10 x BufferB1ml 11 TaKaRa PCR 10 ml 3 12 ml 30 1 1 1 2 PET10 ml m 100L 2 PET o gt 1 PET10 ml 2 ml LLL cis 2 IixBufferA1m 9 up _ O 1 5 ml
10. G C 60 75 95 C B PCR FRZ PCR PCR TaKaRa PCR DD 0 2 ml DNase RNase PCR Thermal Cycler Dice Real Time System II 96 Thermal Cycler Dice Real Time System Thermal Cycler Dice Real Time System L e PCR 48 TP900 xu Thermal Cycler Dice Real Time System e TP700
11. 19 3 30 033006 24 3 2 0302 2 4 PCR PCR PCR Thermal Cycler Dice Real Time System ZZ Cycleave RT PCR Cryptospor rd ium 18S rRNA Detection Kit Cycleave RT PCR_6 2 2 188 rRNA Detection Kit I 3 1
12. PCR 25 Tahaa 26 PCR PCR 1 DNA 2 4 8 L L L1 L1 L1 Li PCR DNA PCR PCR PCR
13. 3 1 2 17 TaKaRa PCR L L1 L1 L1 LI 3 Cycleave RT PCR Cryptosporidium 18S rRNA Detection Kit Cycleave RT PCR Giardia 18S rRNA Detection Kit Positive Control Positive Control EASY Dilution 1 2 1 N 2 1 1 X 10 copies 1 Positive Control 2 1 X 10 copies 1 Positive Control 5 1 EASY Dilution 45u 1 3 1 X 10 copies 1 2 1X 10 copies 1 Y bul EASY Dilution 45u 1 4 1X10 copies wl 3 1X10 copies u 18 bul EASY Dilution 45u 1 5 10 copies wl 4 1X10 copies ul X bul EA
14. C BRAM PCR 1 2 lt 10 1 20 1 200 u 1 1000 1 107 1 10 1 1 TaKaRa PCR 1 5 ml
15. PC Cycleave RT PCR Giardia 18S rRNA Detection Kit Giardia Positive Control 23 TaKaRa PCR IV vol FE z dz X N DNA RNA DNA RNA PCR RT PCR DNA RNA PCR RNA DNA RT
16. 50h 50u 10 5 2 RI CUCRUVS 14 Tala H a PCR L L1 L1 L1 L1 6 DNA RNA RNA PCR RNA cDNA PCR 1 e e Positive Contro1 a Q 5 X PrimeScript RT Master Mix 2u1 O RNase Free dH20 6ul AE TAS 2ul 10 1 1 5 1 10 1 RNase Free dH20
17. PCR 1 3 2 12 Ta hd H a PCR L L1 L1 L1 L1 4
18. 2 I rr RR AB Irc 5 1 2 III oo f RR RR RR RR RR 3 f f ron s 10 1 2 3 4 5 6 D PCR 8 IV 24 V RR nn mn A ex ce 30 TaKaRa PCR L L1 L1 L1 LI Tala R a PCR L L1 L1 L1 L1 L 1 P c
19. DOA 55C 30 sec 3 DNA DNA 72 C 1 min kb 4 DNA 1 1 4 1 25 30 DNA DNA d PCR PCR PCR PCR PCR PCR PCR PCR PCR PCR
20. a 5 30 1 6 T 4 0 0196Tween 80 0 1 M 1M TE buffer 10 mM Tris HCl pH7 6 1 mM EDTA 0 zd T T 0 0196Tween 80 B t idi 0 1 M 50u i po Vortex Vortex 10 f 10 1035 fE 0 0196Tween80 mi 0 1 M 500 I Vortex UE 10 10 10
21. 8 0 2 ml 0 2ml 8 strip tube individual Flat Caps NJ600 0 2 ml 8 8 1 5 ml 0 2 ml PCR 1 5 ml 0 2 ml L L1 L1 L1 L1 TaKaRa PCR
22. 2 Cycleave RT PCR Cryptosporidium 18S rRNA Detection Kit Cycleave RT PCR Giardia 18S rRNA Detection Kit RNA DNA PCR RNase HH RNA baT RM 5 RNase H pemenm RNA DNA DNA t q Wn 2 2 T Hh E MM 44 E 3 RNase H 4 14 V ern 0 708m 44r
23. ROX Ct T Y ri TE An T n A rawy Ora prr rPET 1458 7 B Ll H 4 e 1 er was 3 amp 3 my iim Lei t gt ARDI 25 bas Y Uo T E Rmo Lil minat u san 4 5 5 8 G a eth ul ON PETA th 044 Feii D 4 amp 7 3 9 011 02 H 5 a E G T LJ F OX KK ux www G DK E x wx xxLuUxx x xXx xx x X X x P X X X X X Xx H Negative Control kUPositive Control FAM ROX Negative Control Ct Ct OK Positive Control Ct Ct OK TaKaRa PCR A dd gt Pn 00000 Positive Control 10 45 Tr A a s PENZE 0 2 4 8 S8 10 12 14 16 19 20 22 24 28
24. 16 TaKaRa PCR i tidal s p 1 o k Positive Contro1l Negative Contro1 2 X Cycleave Reaction Mixture 12 541 Q Crypto Primer Probe Mix FAM ROX 5 0 1 O RNase Free dli 5 pA Positive Control 2 091 RNase Free dHz0 25 0 1 x 2 X Cycleave Reaction Mixture Giardia Giardia Primer Probe Mix FAM ROX 10 14 2 X Cycleave Reaction Mixture 175 1 OQ Crypto Primer Probe Mix FAM ROX 70 rp 1 O RNase Free dH O 77 ul 23 uld o4 HE o Negative Control RNase Free dH20 2 1
25. 23J 2 Ue Z EUH TaKaRa TEL 077 543 6116 FAX 077 543 1977 TEL 03 3271 8553 FAX 03 32 71 262 TEL 077 565 6969 FAX 077 565 6995 1209
26. PCR Cryptosporidium parvum Giardia lamblia 7 intesti nalis amp t l amp G duodenalis 24 3
27. PCR L L1 L1 L1 LI RT PCR Cycleave RT PCR Cryptosporidium 18S rRNA Detection Kit CY230 O n PE e o RE 25u 1 50 5 X Primescript RT Master Mix 2 X Cycleave Reaction Mixture Crypto Primer Probe Mix FAM ROX 250 1 RNase Free dH 0 Lysis Buffer Proteinase K dH 0 for Dilution Crypto Positive Control EASY Dilution for Real Time PCR 100 1 625 u 1 ml 625ul X 4 50 1 150 1 50 1 1 ml X 2 PCR Cycleave RT PCR Giardia 18S rRNA Detection Kit CY231 10 25u 1 50 Q 1 5 X PrimeScript RT Master Mix 100 1 2 2 X Cycleave Reaction Mixture Giardia 625 1 Q 3 Giardia Primer Probe Mix FAM ROX 250 1 O 4 RNase Free dH O ml um Q 5 Lysis Buffer 625ul X 4 5 6 Proteinase K 50 1 wl T dH O for Dilution 150 1 PCR Q 8 Giardia Positive Control 50 1 Q 9 EASY Dilution for Real Time PCR mlX 2
28. cDNA PCR PCR PCR PCR Polymerase Chain Reaction DNA denaturation step Tannealing step extension step in vitro DNA 1 DNA 10 s 1 3 5 1 4 1 2 1 2 3 25 30 5 3 EM SZ 94 4 DNA g xj j 10 3 4 C n 3 554 4 min 1 PCR DNA 24 TaKaR d PCR DD 1 dNTP DNA AB 94C 30 sec 2
29. Sample 1 1 58 Sample 2 3165 Positive Control Eu 21 TaKaRa PCR Ct Crossing Point 2nd Derivative Maximum 2 Crossing Point CP Positive Contro1 d Ww 23 50 4 45 4 40 TN 35 1 50 4 20 20 15 4 10 4
30. PCR 2 PCR LAMP 1 rRNA rDNA rRNA PCR LAMP e 4 Ct PCR Ct Ct gt Cycleave RT PCR Cryptosporidium 18S rRNA Detection Kit CY230 Cycleave RT PCR Giardia 18S rRNA Dete
31. H A lt 50 y l E 100 p I f NN 1M 10 c 7 TE buffer 200y 260u 12 000rpm 50u TE 10 mM Tris HCI pH7 6 1 mM EDTA 20 13 TaKaRa PCR L L1 L1 L1 LI 5 2 lt cvi XvCv 7X mWeB dsiO0Proteinase K e Proteinase K R 4 Proteinase K s Proteinase K tu tu l dH O for Dilution 3p OQ Lysis Buffer 49yu 80C 37C 5
32. 5 PEES TaKaRa PCR L L1 L1 L1 L1 PCR Threshold Ct Threshold Cycle x Ct Posi Ct Nega a auzas E mox EST FRE o end 69 3 PRT Pnmaiy Curve bd i 5o am banno M M he Eus a p 3 amp amp 7 8 653112 E Threshold F rasis e7 snow k 20 f Z ww gt _ N N a x T Pi y 8 Ww w w ww Ww UZ X Ww x E 10 P n r CO A Y wo Wow a EGO KK T x lgo w x w x x 3 INE UND D M LJ E OG ND MD dE 0 2 4 6 8 10 12 M 16 18 20 22 24 26 28 20 X2 4 36 IM 40 42 44 46 48 50 OO LL COO XC 7
33. PCR yO RNA Synthesis of first strand cDNA WhPrlmeScript RTase cDNA Second strand cDNA Synthesized with PCR components added TaKaRa Ex Tag HS T s Amplify CDNA TaKaRa PCR DD PCR 1 1 Cycleave RT PCR Cryptosporidium 18S rRNA Detection Kit Cycleave RT PCR Giardia 18S rRNA Detection Kit Thermal Cycler Dice Real Time System A
34. 9 50 72 C 20 30 10 20 50 19 TaKaRa PCR L L1 L1 L1 LI 8 20 XE TERT CU gPCRIXOUG HR KL AM CA EORR AME XdVCwv 228E amp 88 UL Positive Control Positive Contro1 FAM Ct Ct ROX Negative Control Ct ROX Ct FAM Ct
35. IMER eb anh Cycleave PCR 6 3 CY218 78 000 30 LA Quencher This product is for measurement of amplification detection for research use only in life sciences research industrial and environmental testing including food industry but excluding bio terrorism and bio warfare non human animal diagnostic testing forensic testing and providing services to third parties who do not use the services for the purpose of a providing patient management or care and in which the results of such services are not included in patient records and b providing bio terrorism or bio warfare testing and specifically excluding without limitation I any human clinical therapeutic or diagnostic uses and animal clinical and therapeutic uses including any use of the results of any testing performed with any product for patient management or care or the use of the results of the services for patient management or care and II any research or services where the results of any test or assay are used for patient management care or otherwise in making therapeutic or treatment decisions for a patient portion of this product is subject to proprietary rights of Epoch Biosciences Inc and are made and sold under license from Epoch Biosciences Inc under the pat
36. 28 Takah d PCR L L1 L1 L1 L1 DNA RNA C7 DNA Cycleave RT PCR Cryptosporidium 18S rRNA Detection Kit Cycleave RT PCR Giardia 18S rRNA Detection Kit Positive Control 29 Ta hd R a PCR V PCR P 96 E Thermal Cycler Dice Real Time System PCR TP900 Y3 750 000 T 48 Thermal Cycler Dice Real Time System e PCR TP700 Y2 300 000 WUTJ 54 h PCR O 5 Ri E vy J 1 0 2 ml 8 stri
37. 28 30 32 34 36 38 40 42 44 48 43 50 45st Ct taoa IRR Hej I R2099 Eft 1036 Y 3239 LOGHI 38 43 0 i w 000 10009 100000 1000000 ats er Ct Plasmid ROX E a eo 8 Ct Sample Sample Sample Sample 2BUE DDU 2 556E 001 2 806E 000 200 0E 0D0 2 820E 0D0 2 B BE DUD i 2 000E 001 2 052E 001 2 5256 001 2 nHE H01 2 898E 0D1 2 525E DU1 c ER 2000E 002 2 732E nn2 2 618E 002 AE zz 1 8 2 000E 002f 2 5085E 02 2 618E 002 2 000E 003f 2 178E 003 2 157 E 003 2 000E 003f 2 135E 003 21576 0043 2O0E U4I 1 958E 004 1 901 E 004 2 00Q0E 004 1 845E U04 1 301E n 04 eg HHHE HHBI 1 692E 005 1 697 E 005 2 000E 0054 1 703E 005 1 697 E 005 HE Positive Control JIE
38. 40 a S C SC S E y COE CY Thresho1d CE Ct Thresho1d CER Ct min ammo e N gt lt P gt OK Yo OUT RRR RR sample lt U gt ESSES Posi Nega ND Error Thermal Cycler Dice Real Time System 77 27 TaKaRa PCR DD DNA db PCR DNA LLL P E assi 7RBQfi Threshold tonm Ct Threshold
39. 5 Q 4 l 00E 05 1 00E 03 1 00E 01 1 00E 01 1 00E 03 1 00E 05 M or Copiesytube 1 433ln x 38 67 R 0 9992 X HESA Wii Ctf y7 1435ln x 24 605 E 0 9676 1 18S rRNA 18 000 PC Cycleave RT PCR Cryptosporidium 188 rRNA Detection Kit Crypto Positive Contro1 22 TaKaRa PCR E Dl D D Lucr y 7 138Tln x 38 363 R 0 9989 wo rm o m zi P A i F il 25 7 x sjal EA i ea 20 balja 15 1 424ln x t 280 269 RE 0 9079 10 M UE 1 00E G3 lO00EQO1 1 00E 01 1 00E 03 1 00E 08 or Copes gqPCRR I a7 35 79 1 18S rRNA 1 600
40. Cycle PCRE R U 3 Ct Ct v RONAN Logit Ct ENES mips 75 NIE 5 DB Coi c J e im zanin s pe Pv mmm xcd T EE 2j Sx y i4 En QUOI Uma Ta E L t D a ag E oss xoc ut Ct aa ht Urt CLET 1 on xs o 9 37 228 ST NEAMEN 66x 2 PEEDU CIECA CCP FARR NTC FAM E B6 UNKN FAM 33 64 2190E 001 TE gr C6 STD FAM 34 882 1000E 001 9 749E 000 D6 STD FAM 3136 1000E 002 1 046E 002 i E6 STD FAM 28 08 1000E 003 9 925E 002 F F6 STD FAM 2472 1000E 004 945E 003 Thermal Cycler Dice Real Time System 77
41. NA 18S rRNABUA 2e d DET 1 2 PCR uo AMNES FAM d i 1 gt t4 em e M y 1 amp m lo WE d X oW o4 Ww lt T N E ommo ROX 7 I ESES PERSEA TEE EEA A CT TE A ae PCR DNA DNA PCR
42. SY Dilution 45g 1 6 1 copy ul 5 10 copies u 1 Y amp RW bul EASY Dilution 45 1 T 1 1 5 ul 1 u l 1 l l Lx 10 1x10 EN 3 Positive Control 2 1 Thermal Cycler Dice Real Time System 77 3 Ver 1 0 18 TaKaRa PCR et0f2 Cycleave RT PCR Cryptosporidium 18S rRNA Detection Kit Cycleave RT PCR Giardia 18S rRNA Detection Kit Cycleave RT PCR Cryptosporidium 18S rRNA Detection Kit Cycleave RT PCR Giardia 18S rRNA Detection Kit we ORE R2 CycleavePCR Kit I PE DESA ane SNPs 4 P PCR Cycleave RT PCR Cryptosporidium 18S rRNA Detection Kit 95 C 10 5 95C 5 HuntmnHk CycleavePCR Kit x E mF A Hu IR e Ei ZE 55C 10
43. ction Kit CY231 18S rRNA 18S rRNA DNA RA RNA cDNA DNA RNA RNA PCR Y RT PCR Korm 23 RT PCR PrimeScript RTase RNA cDNA TaKaka Ex Tag HS
44. ed non transferable license for all fields other than human or veterinary 7 vitro diagnostics under specific claims of U S Patent Nos 6 174 670 6 569 627 6 303 305 and 6 503 720 owned by the University of Utah Research Foundation and licensed to Idaho Technology Inc and Roche Diagnostics GmbH 2012 9 1
45. ents and patent applications US20020155484 W00142505 W002099141 US10 113 445 US09 876 830 and corresponding patents issued in other countries here is no implied license for commercial use with respect to this product A license must be obtained directly from Epoch Biosciences Inc with respect to any proposed commercial use of this product and commercial use includes but is not limited to A the sale lease license or other transfer of this product or any material derived or produced from it B the sale lease license or other grant of rights to use this product or any material derived or produced from it and C the sale lease license or other transfer of kits which include this product L15 Hot Start PCR Licensed under U S Patent No 5 338 671 and 5 587 287 and corresponding patents in other countries L34 amp cereus This product is covered by the claims of U S Patent No 7 264 959 and its foreign counterpart patent claims L42 Campylobacter PCR Detection This product is sold by Takara Bio Inc under license from Fuso Pharmaceutical Industr ies Ltd and Osaka Prefecture University This product is covered by the claims of U S Patent No 7 563 594 and its foreign counterparts L 45 Heated Cover This product is covered by the claims of U S Patent 5 552 580 and their corresponding foreign counterpart patent claims L47 Real Time PCR Quantification Method The purchase of this product includes a limit
46. p tube individual Flat Caps Y 2 78 9 87 2 MA600 18 000 lloycleavePCR lt gt See 0 0 000 Gycleave RT PCR Cryptospor idium 18S rRNA 18S rRNA SIZE CY230 50 67 000 Detection Kit Cycleave RT PCR Giardia 18S rRNA 18S rRNA CY231 50 67 000 Detection Kit gt ER 0 E Cycleave PCR 0 157 VT gene Screening Kit Qe AT VTI VI2 GR Cycleave PCR 0 157 VT gene Screening Kit 50 Ver 2 0 CY217B 100 93 000 Cycleave POR 0 157 VTI VT2 Typing Kit e m aa ARR 50 e URN Salmonella Detection Kit Cycleave PCR l brio tadhgene Detection Kit tah CY220 50 Y67 000 OVC LEAVE FER Bacillus cereus URS gene CY221 50 Y67 000 Detection Kit Oyceave POR Listeria monocytogenes inlA Internalin A n A CY223 50 Y67 000 gene Detection Kit 5 Cytolethal distending toxin dad Campylobacter Jejuni AJ col I C CY225 50 Y67 000 yping I cdtC gene House d RC nud aureus Dnay Keeping CY228 50 y67 000 gene Detection Ki DnaJ 20

リアルタイム PCR によるクリプトスポリジウム、ジアルジア

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リアルタイム PCR による クリプトスポリジウム、ジアルジア

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リアルタイム PCR によるクリプトスポリジウム、ジアルジア