シカジーニアス コアグラーゼ検出セット(黄色ブドウ球菌用) 取扱説明書
Contents
1. 10u L 3 72 C 6 94 C 3 4 15 000rpm 1 DNA 2 PCR 1 2 PCR 2 1 2 PCR DNA A B 2 0 EagleTaq Master Mix 10 0 6 0 20 0 IN IT IT lt PCR 4 C 94 C 4 94 C 30 52 C 90 30 72 C 60 72 C 7 3 1 TBE 3 2 PC2. 5 20C 8 1 2 9 08178 96 DNA 120 717678 5 LB Miller 500g 711129 5 500g 01089 23 KANTO HC 100g 46510 79 10xTBE 1L 14575 43 2mg mL 10mL 49881 00 100 bp DNA Ladder 100 103 0022 2 03 6214 1090 54 0048 2 5 1 812 00
3. A B B 1 PCR 1 10 1 M 100 bp DNA ladder A A IV VI WL 2 B BB 1 II V VI 4 1 NBRC COA A B NBRC102135 I 644 bp NBRC102136 I 342 bp NBRC102137 310 bp NBRC102138 WV 490 bp NBRC102139 V 482 bp NBRC102140 VU 269 bp NBRC102141 MI 217 bp NBRC102142 WL 358 bp NBRC102143 NBRC102144 Xx femA 132 bp 132 bp NBRC NITE Biological Resource Center emA PCR 12 155 99 496 154 155 2 2 PCR COA I I LL IV V VW
4. 1 0 mLx1 EagleTaq Master Mix 1 0 mL x 1 1EagleTaq Master Mix Roche Diagnostics K K 4 I lt PCR 5 1 gt W 6 1 DNA DNA DNA DNA DNA DNA 1 DNA 100u L 2
5. VI N T I 3 I 23 _ 12 mW 7 1 Vv 5 VI 73 VI 21 WI 10 Total 3 23 12 7 5 73 21 10 1 NT I gt lt F541 0048 28 962sie72 tp www kanto co jp 812 0007 2 22 3 092 414 9361 e mail reag info gms kanto cOo jD
6. 07 22 3 1 5 mL UV 0 2 mL PCR 1 5 mL 20 u L 200 u L 10 Sakai F Takemoto A Watanabe S Aoyama K Ohkubo T Yanahira S Igarashi H Kozaki S Hiramatsu K Ito T Multiplex PCRs for assignment of Staphylocoagulase types and subtypes of type VI Staphylocoagulase J Microbiol Methods 2008 75 2 312 317 http www kanto co jp e mail reag info gms kanto co jp 06 6231 1672 092 414 9361 T7315 2 22 12 Cica Geneus Staph Coagulase Detection Set 11 PCR M 1 2 3 W0 7 SD0 IM 1 2 3 5 6 9 0 A
7. R 4u L C 3 6u L 4 100V 30 100bp DNA Ladder 4 0 5 ug mL 30 UV 5 DNA 1 7 1 2 DNA 10 100 ng h L 3 DNA 1 DNA 4 PCR 50u L
8. T7315 2 22 12 Cica Geneus Staph Coagulase Detection Set 1 Staphylococcus aureus COA 1 2 PCR PCR 1 2 Cica Geneus Staph Coagulase Detection Set 08179 96 50 20 3 50 A IL IV VL ML 4 0 1 mLx 1 B B 1 IH V VI 4 0 1 mLx 1 C 6x
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