『 レビス ® 高分子アディポネクチン‐マウス／ラット 』取扱説明書
Contents
1. 3 80mg dL 80mg dL 80mg dL 2 PP PE2. 1 2 0 1 1 ELISA 20 25 0 4m sec 30 9
3. 50 25 50 25 1 10 10 1 490 240 35 11 1 3 lt 2 50 1 3 50 1 4
4. 450nm 620nm 5 ELISA 1
5. C E CTMB 2 8 C D HRP 2 8 C H LM HzS04 2 8 C L 10 10 x 2 8 C 9
6. Vortex 600 1 200 rpm 96 Web 96 450 10 nm 620nm 600 650nm 8 20 25 C 2 6 B 2 000 ng m1 B 2 000 ng ml C ng ml 501 450 1 200 200 ng ml 2001 200 1 100 100 ng ml 2001 200 1
7. HMW BMTI 2 HMW DM2 ELISA HMW 4 2 HRP 90 HRP TMB
8. 6 3 adipocytokine adipokine 3 6 12 18 3 LMW C1q 3 6 MMW HMW
9. 5 20 25 C 2 6 3 lt 7 HRP 50 1 8 20 25 90 9 3 G lt 10 50 1 CK 11 20 25 C 30 12
10. HRP 5041 20 25 C 90 gt 3 TMB TMB 50g1 20 25 30 1M HzSO 50 1 450nm 620nm 600 650nm a 2 10 3 Saat 300 ux1 3 13 ng ml OD OD
11. 1 1 96 0 4m sec 30 Web 10 35 C
12. 1 3 ea 4 6 5 25ml 600 1 200rpm 10 3 7 1 2 3 4 5 6 7 8 9 10 11 12 A B C D E F G H
13. AKMAN 011 Mouse Rat High Molecular Weight Adiponectin ELISA KIT AKMAN 011 Shibayagi Co Ltd Gunma Japan 377 0007 1062 1 TEL 0279 25 0279 FAX 0279 23 0313 lt E mail gt syc info shibayagn co p lt URL gt http www shibayagi co jp
14. 3 18 200 ng ml ELISA 2 Mouse Adiponectin HMW 100 Rat Adiponectin HMW 100 Mouse Adiponectin Hexamer lt 5 1 000ng ml 1 000 ng ml Mouse Adiponectin Trimer Mouse Adiponectin Monomey Mouse MCH Mouse TNF a Mouse INE y Mouse Insulin Mouse Leptin Rat Adiponectin Monomey Rat TNF a Rat INFE y Rat Insulin Rat Leptin 5 5 2 C V 5 2 3 4 C V 5 2 3 94 4 105 2 3 R2
15. ng m1l C a Ne oy 1 HMW Adiponectin 3 4 100 ngiml Safire2 TECAN 13
16. 2 3 Q 1 A 1 9Q 2 A 2 Q amp A 16 Web Web
17. 2014 2 10 Web Q amp A 1 4 1 96 2 2 8
18. Q amp A 20 lt 25 C 2 10 ng ml 12 5 6 25 3 13 0 1 50 0 0 mr 200 200 u 1D 450 200 200 200 200 200 200 200 96 3 501 20 25 C 2 HRP 100 3
19. 50w1 13 450nm 620nm 600 650nm 7 Strip 1 amp 2 Strip 3 amp 4 Strip 5 amp 6 Strip 7 amp 8 Strip 9 amp 10 Strip 11 amp 12 A 200ng ml 8 16 24 32 B 100ng ml 1 9 17 25 33 C 50ngml 2 10 18 26 84 D 25ng ml 3 11 19 27 35 E 12 5ng ml 4 12 20 28 36 F 6 25ng ml 5 13 21 29 37 G 3 13 ng ml 6 14 22 30 88 H 0 7 15 23 31 39 12 1 X ng7ml Y Web ELISA
20. 0 999 14 iA ng m1 a SD BALB c mouse 6w 9 10 2 369 743 ICR mouse 6w d 10 2 119 802 CD rat 8w 9 3 220 670 15 Q amp A 1 2 3 4 5 6 7 3 13 ng ml OD OD 3 4 6 CV 1
21. 0 4m sec Web 6 A 96 96 wells 8x12 1 B 2 000 ng ml 200 1 1 C 60m1l 1 D HRP 100 1 1 F TMB 12ml 1 H 1M HzSO4 12m1 1 1 10 x 100m1 1 3 1 7 510 1 50 500 1 Eppendorf multipette plus 50 1
22. 50 50 ng ml 2001 200 1 25 25 ng ml 2001 200 1 12 5 12 5 ng ml 2001 200 1 6 25 6 25 ng ml 200 1 200 1 3 13 0 2001 0 D HRP 100 1 C 100 Q 10 x 10 x 10 100ml 10 x 900ml 96 A 96 2 8 C B 2 000 ng m1 2 8 C
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