Untitled - Bio-Rad
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1. 2 0 0 3 0000000000000 00000000000000000000000000000000 0 0 00000000000000000001 0 00000000000000000000000000000000001 0 00000000000000000000000000000000001 0 0 00000000 4 0 000000000002. 00000000000000000000000 l 000000001 00000000000000000 10050 1000000000000 1 000000000000000000 0 0000000000000000000000000000000 0 25 0000000000 B eg Sigma histopaque 0 00 0000000000000 5 50 0 c 00000000000000000000000000000000000 0 00 0 00000000000000000000000000000 2 4 q 00200000 3 000000 PBS 2x10 000000000000000000000 000000000 eghReichert inc 0000000000000 4 0000000000000000000000000000037 10000 5 000 0000 141 000000000000000 45 000000 6 000000000000000000000000000000000 0 00000000000000000000000000 7 0000000000000 49100000019 200000000 8 000000000 3 50 00 05 EDTA pH9 1 Sarcosyl 05mg ml KO Boeringer mannheimp 00000 0 9 0000000000 50 20000000000000000000
3. 0000000000000000000000 note 1 0000000000000000000000000000000000000000000 100000000 000000 0 00000000000000000000000000000000000000 0000000000000060 000000000000000 L 00000000000 35 000000060 0 0000000100 0000000000000000 1 215 00000000000000 00000 05 090 0000000000000000000000 0 000000000000000000000000000000 0 0 00000010 0 0000000000000000000000000000 0 81 0 0 E ET ET l L3 L3 E3 E3 EJ EJ E Imb 000000000000000000000000 00000000 162 0133 0000000000 0000000000 0000000000000 02 00000000000000000000000
4. Hl 0000000000000000000 0 2x 10x0 0000000000 200 200 0 00000 1 0 5x 1 0x TAE TAE Li bk u a 0 4 wt Nalaciby temuhr DNAD 0 00000000000000000000000000000000000 00000000000000000000000000000000000 000000000000000000000000000000000 0 000 0000000000000 EZI q Jj J L3 L3 L3 q L3
5. 0000000 00000001 000000000000000000000000000 000000 10 159 000 0 00000000000000000000000001 0000000000000 0000000001 0 0000000000000000000000001 LO BLELBL BL BL BL EE 7 H 10 0 2 2 0 000000000000000000000000000000000000 000000000060000 Lambda Ladder 5 cerevisiae H wingei 5 pombe Lambda ladder was separated on a 1 0 Molecular biology Certified Agarose catalog number 162 0133 gel in 0 5x TBE recirculated at 14 C The run time was 22 hours at 6 V cm with a 50 to 90 second swich time ramp at an included angle of 120 Saccharomyces cerevisiae Strain YNN295 Chromosomes were separated on a 1 0 Pulsed Field Certified Agarose catalog number 162 0137 gei in 0 5x TBE recirculated at 14 C The run time was 24 hours at 6 V cm with a 60 to 120 second switch time ramp at an included angle of 120 Hansenula Wingei Strain YB 4662 VIA Chromosomes were separated on a 0 8 Pulsed Field Certified Agarose gel in 1 0x TAE recirculated at 14 run time was 48 hours at 3 V cm with 500 second switch time a included angle of 106 Schizosaccharomyces pombe Strain 972 h Chromosomes were separated on a 0 6 Chromosomal Grade Agarose catalog
6. Corning Pyrex 1 00000000 28x18x4cm B0 0 0000000000000000000000 glass 0000 0 000000000000000 90 000000000000000 00000 CD O0 DU D D U 16x20cm 500000 200000000000000 18x30cm amp 5 68004 0 000000000 Zeta 00000000000000000002 000000 10000000000020 lj 18x15cm S amp S 680021 1070 000000000000000000 5 24481 e 00000000000 7 05M Tris 70 000000000000000 50 00000000 2 556 00000 0 0000000000000000000000000000001 0 000000000000000 aa 0000 0 0 0 DD 2 0009 0000000000000 900000001 Mapper 00008 S Cerevisiae 00 0000000 0 0 200 1
7. 000000002000000000 5000 31000000000 0200000000000 4 0000000 50 0 0 mtr 000000 PMSF phenyl methyl sulfonyl flouridd 0 0 0 2000200000000001 000000000000 100 2004 0000000000000000000 00000000000000 10 204 0 100 00000000000001 0000000000000000000 000000001 1 9 22000 000000000000001 0490000000000000000000200000000000001 000000000000000000000000000000000001 000000000000000000000000 10 05 0000000000 9000000000000001 000000000 08 000000000000000 0000000 301 0000000000000000000000 1 4 0000000000000000000000 000000000 0 000000000000000000000000000 1 000000000
8. Lai E Personal Communication 000000000000000000000000000000000 6 0 0000 DNAD gt 3mb 0 00000000000000000000000 0000 0 000 00 J J EST O 0000000000000000000000000000000000000 00000000000 0000241000000000000000000000000000002 L 0 Lai Clarkand Hood Science 241 1203 1205 19880 0 0 0 120 9 000000000 PNAQ0000000 0000 00 0 DNA gt 0 0 0 000 00000 000 0 00 0 0 000 0 30 0 S Cerevisiae ES IE 0 00000000000000000000000000000000000 0 0 q J 0 lt 120 00000000000 J L3 r3 L3 L3 L3 L3 E3 J q q 120 105 100 96 945 0000000060000 0000000060000 0000000060000 0000000000000
9. 0 01M T ris pH7 3 7 5 mercoptoethanol 0 00000000000001 0 0000 00000 0000000 0 5M EDTA 925ml Trizma pH7 5 1 59 mercaptoethanol 75ml 1L 00000 37 000 14 241 00000 00 00 0000 12 300 250 0 0 05M pH84 00000 NDS 0 01Mtris 7 50 0 45M pH8 0 1 laurylsarcosine 1mg ml Proteinase 0000000000000 0 13 50 00000000000000 194 2400 14 NDS 0000000000000000 005M EDTA pH80 15 0000 15 0000000 00SM 801 000000000000000000 16 0 05M 17 0100000000 005M EDTA pH80 4101000000000 170 3593 000000000 1 3
10. CHEF Mapper CHEF DR III CHEF PJ2Uy 132Z 37 b ver 1 1 Ar Eb BEL ai a ua k i m va CERE Eo iEn eR BS De u ul 12 Saccharomyces cerevisiae LB I 13 upo pA p u u i u us R Pg UR EE E MER DE RR w u TD 21 OD pO Sa yaaa susya 22 O aa uu a ass T B I Zaa n UII 5 asua aaa su a ua s uuu sa CI 11 000 0000000000 Technical Bulletin 000000 00000000 Bulletin 1589 Preparation and separation of mammalian DNA by PFGE Bulletin 1624 Cooling module Bulletin 1637 CHEF Mapper and CHEF Mapper XA system specifications Bulletin 1647 Electrophoretic Karyotypes of Wine Strains of S cerevisiae Bulletin 1648 Application of two dimensional PFGE for determining molecular Karyotypes Bulletin 1659 Extending the upper limits of PFGE using programmed voltage gradient Bulletin 1736 PFGE analysis of Chromosomal DNA from a Single hair root Bulletin 1753 Preparation and restriction digestion of E
11. 00 0000000060000 03 oi mi ul ii N A 1000000000000000000000000000000000 1 10 9 9000 04N NaOH 15M NaCI 150 0 0 0 0 0 DNA 200 NaOH 15M NaCl 0000000000000 000 0 0 00 0 0 0 000 1620196
12. 00 00000 9000 M3113 0105
13. EJ E3 m 000000000 20XSSCU 0000 00 0 0 0 00000 00 00 0 00000 000000000000000000000000000000000001 00000000 00000000000000000000000000001 0000000 000000000000000000000000000001 0 00 CHEF 00000 DNA 0000000000000000 1 0000000000000000 5000 00 0000 00 0000 0 0 165450007 1000000000000000000000000010000000001 I DNA 0 00000000000000000000000000000000000 0000000000 000000 0000000000000000000000000000000000001 000000000001 0 0 0 0000000 0 00000000 g m 0000000000001 0000000000 0000000000000000000 Iz ekim aj 000000000 00000000000 00000000 D u 00005000 00000000000550 00000000 2100000049000 00 0000 9000 000 000000 00009000 0000000000955 0000 7E 000 0009000 FAXQ 000 0000 8000 000000 00009000 0000000509 000000 0000 009000 FAXO 0000 000 9000 00000 00005000 00000000005050 000000 0000009000 FAXO 00 00000 9000 000000 00009000 00000000 009009 MIDORI 0 000 0009000 FAXQ 000 0000 8000 00000 0005000 00000000000408 000000 000 0009000 FAXO 000 0000 8000 0000000000 2510000005000
14. Coli chromosomal DNA in agarose plugs for use in PFGE Bulletin 1769 Resolution oflinear DNA fragments from 23Kb to 6Mb using biphasic linear Switch Time ramping Bulletin 1796 CHEF DRIII variable angle PFGE system Bulletin 1584 M785 vacuum blotter Bulletin 2096 Zeta probe GT membranes 11000000 11 000000000000000000 00 000000000000000000000000000000000000 00000 1703719 00000000000 0 000000000000000000000000000000001 00000000000 0 162 0017 0000000000000000001 0 00 00000000000000000000000000000000001 0000000000000 0000000000000000000000000 DNA 0 0000000000000000000000000000000000001 0000000000000 50 200 00 00 0 0 0 0 0 0000 0000000000000000000000000000000001 075mm 0000000000000000000000 1 2 Sa
15. 000000000000000000000000 210000000000 CHEF 0 000 0000000000000000 000 14 13em 00000000000 21cmx 0 14emx 21c 000001 1 00000000000000000000000000000000000001 0 00000000000000000000000000000001 00000 000000000000000000000000001 0 0 000000000000001 0 00000000000000000000000000000001 uuu N 10x TBE 0 5Ox TAE 108g Tris base 242g Tris base 55g Boric acid 57 1ml gracial acetic acid 40ml 0 5M EDTA pH8 0 100ml 5 pue LU HD 0000 HO L wies S pto md 00000000000 3 00000000000000000000000000000 1704048 60 000000000000 00000001 000000000000000000000001 30450 0000000000000 0000000 0001 000 100ml 14 80 2 1 0000000000 00000000000000000000 00000000001 0000 900 0000
16. 000kb 000000000 000000001 000000001 000000000 302170 UVI 0000 000000000 000 Polaroid type 661 0000000001 0 0 0000000000000000000000000 0 amp 41000000000000000000 25410 50 100 15 300 450 641 90 3000 00000 0 0 0 0 0 0 000 59 00000000000 0 0000000000000000000005000000000000001 00000000000000000000000000000000000001 000000000000000 190 III 302 0 000000000000000000000000000000000000 10 00000000000000000000000000000000000000 0 0000000000000710000000000 as 00000000 3 000 0000 00000000000000000000000 0000 0000000
17. ccharomyces cerevisiae 000000 Schwartz and Cantor 1988 00 0000 0 0 00 00 00 0 0 000 00000 1 Y PD 1 Y east Extract 2 2 0 0000 1 30 36 4810000000 2 4010 3000rpn 000000000000 3 0 05M EDTA pH84f 1 0000000 20000000 4 0000000000000 0 05 801 00 0 0000 00000 0 5 0000000000000000001 6 Lyticasd 2 0000000000000 00 201 30 7 0125M pH750 0 0 11 000000000000000000001 0 0000000000000000000000 45 59 000000 8 000000000000000 00 45 590 0 0 00 0 0 00 0 29000 0 000000000000000000 0 75 179 0000000000001 0000000000000000 0 05 EDTA 0 80 000000001 9 00000000000000000000000000000000001 0 49 20000000000 10 000000000000000000000000000000000001 0000000000000000000000000 00 046M EDTA
18. number 162 0135 gei in 1 0 TAE recirculated at 14 C The run time was 72 hours at 1 5 V cm with a 30 minute switch time at a included angle of 106 01 30 Wu 0 000000000000000000000000000000000000 2mm 0 000000000000000000000000000000000000 30 00000 24 00000000000000000000000000000000 0000000000000 45 00 0 0 00 000 000000000000000 149 000000000000000000000 0000000 4 00000000000 054 9 70 000000000000000 20300000001 0 0 00 0000000000000000000000000000000000001 230 00 0000 00 0 00 00000 00 0 0 000 00 00 254360m 0 UU
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