PROTEUS Protein A Mini Kit簡易取扱説明書
Contents
1. 0000000C0000 000000000000 o 1000 150x g0 30 00000000000000 0000C00OCOCOO0COO00CO0O0OOO00OO000O OUD 0o00 OO00000O0O0OO 5000000000 00000000000 0O0OOOC O0OO0OO DO 00O O 00 00000000N 6SOOOOODODDODODOD 000000 0 10mL Wash buffer pA7 4 30mM imidazole 0 O O 500x gy 3000000000 O0O0O0OCOOCODOOOOOO0OOOOOCOOOO0O00OO00O0O00 00O 0O00 DOUN D D D 00 3000000 DODO COOL OO OO DU Olmidazole 280m0 0 000000 000000000000 00O mida oeng 0 000 00O Wash bufferg 0 000000000 0000 00000000D O0 OODD 700000000000000 w00 0o000oO C O OC OOCOCOCOCOCO OOO0OO0O00 0000000 O 10ml O Elution Buffer pH 7 4 300mM imidazole 1 O 0500x g0 3000000 00000 00000000 Do O00O0O0OO0OOODOOO0OOOO0OCOO0OCOOOOD0O 000000000000 0000D D OO0O O OO OOCOOOCCOOC OOO0O0OO0O0OC0000 00 000000000000 o0o g 000000000000 0000000000 Dono 000 03323 0673 000 t 06 6396 6616 e miil technosales Gtechnosaurus co p 8 0 0000 DO OO OO DONDOO 000 imidazole 0 0000000000 0000000000 0000 000 0000000000 OO OO OO OO imidazole 0 00000000000 00 000000 00 00 20 C 80 0 000000000000000 000000000 0 000 00000000000000 O Apolo 20mL ADa 00 00 000 9 IMAC JO 000000 0000000 10mL 0 Elution Buffer O O 0500xg0 30000000000000 0 000 00 0000000000 10mL O Binding Buffer DO O00 0200000000000 DODO OO OO OO 3000000000000 ua aa aaa aa aaa aaa aaa AA AA aa aaa 00000000 0000000000 20 8400000 000 0000000000 Dono 000 03323 0673 000 06 6396 6616 e miil te2. PROTEUSIMAC Midi Kiti 00 00000000 0 PCM CCC O00 000OOOOO Na DA OO 80 DOD0OOOO0O OOo CODODODODODOO 20m SN semi 0 O 000o 12 0 bx PBS buffer Buffer A 250mL ix imidazole buffer Buffer B 150mL DQODODODO 10 moo ooo ooo 00 000000000000 020 aa aaa aaa aa aa aaa aa p 0020016000 00000000000000 aua aaa aaa aa AA AA Aa OOo UOOOOO Doo 00 00000 24 m0 12u m0 000000000 OODoODoOdcOgOgoODoD 0 Milipore 0 2 m Steriflip GP uni O00 O O SCGP 005 25 0000 10 0000000 O O0OOCO0O0O0OO0O 200000000 000 COCOCODOODODODOUDO O DOBinding buffer Wash bufferfJElution buffer PO 0 000 00000000000000 200000000 0D Imidazole Recommended Buffer A ml Elution buffer B ml Distilled Total concentration mM usage 5 x PBS buffer 1 M imidazole pH 7 4 water ml volume ml 10 Binding buffer 20 1 79 100 20 20 2 78 100 30 Wash buffer 20 3 77 100 50 20 5 75 100 100 20 10 70 100 150 20 15 65 100 200 20 20 60 100 250 20 25 55 100 300 Elution buffer 20 30 50 100 350 20 35 45 100 400 20 40 40 100 450 20 45 35 100 500 20 50 30 100 0000 OO OA Do 000 033235 0673 000 t 06 6396 6616 e mail technosales Gtechnosaurus co p 2000000000 VOU OOOODUODCOOOLDODCONOODODUODODOO DODODCONDUODUDOO 0000000000 300000000000 00000800 10mL Binding buffer pH7 4 10mM imidazolgA 020 0 0 000000000000 Do 0 050029 0300000000 400000000 220 25mL 0 0 000 00124 m00 2u m 0000000 00 0000000000000000 0 000000000 S000000 agu0 g0g wog 0000000000 20m0 O
3. chnosales technosaurus co jp
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