産学官共同研究の効果的な推進 事後評価
Contents
1.2. 25 400
3. 6 9 1 Molecular that homogizes genotype and phenotype and utilization thereof US Patent 6 361 943 B1 2002 Mitsubishi Chemical Cor H Yanagawa N Nemoto E Miyamoto Y Husimi IVV 2 3683282 2005
4. IVV LIVV IVV
5. 1950 3 1 1 g 1
6. 3 160 6 78 4 1
7. IVV IVV ea se 3 TIVV 1 2 Over lap ET CC 0 aw fron SS Y Dr y Ea Dz1f gt y gt eo 6 a 1 1 1 4 a yf ELISA
8. cDNA 0 0 005 0 0 GSNN MO 6 DNA 10 jn vitro
9. 5ml
10. 3 1 2 2 1 2 FA AAR PP 10 0 10 0 3 vitro virus TVV
11. IVV 3 IVV 6 PCR 2
12. 2 6 24 6 2 IVV
13. 5 1 100
14. mRNA IVV 3 3 1 a
15. IVV 1 PCR 2 IVV IVV 1 IVV
16. b 3 30 000Xg b 4 Sephadex G 25 b 5
17. IVV FI 6 IVV PEG mRNA mRNA PEG PCR 2
18. CD3 mRNA IVV 6 IL 6 a TNF a 4 ELISA IL 6 2 TNF a 2 V V
19. 2 4 PCR IVV 72929 IVV
20. DNA DNA DNA mRNA 1
21. 3 IVV cDNA 3 3
22. PCR 2 IVV IVV 2 IVV
23. TFF TFF 2 Step 1 2 4 2 a mRNA SP6 RNA mRNA b GFP
24. 1 6 1 8 1 1 2 3 4 5 6 7 8 2 1 2 8 8 1 2 3 MN FF I
25. 2 4 5 4 6 2 IVV 1 2
26. IVV 17 5 6 8 GFP 3 GST His Reaction time hi kDa 0 2 4 6 10 3 GFP 4
27. 7922 IVV IVV 6 IVV
28. 9 IVV
29. 2 IVV PCR 2 IVV 3 1
30. 3706942 IVV 7 1 1 0 1 0 1 0 3 3 0 6 4 0 5 1 ln vitro evolution of single chain antibodies using mRNA display Fukuda 1 Kojoh K Tabata N Doi N Takashima H Miyamoto Sato E Yanagawa H Nucleic Acids Res 34 e127 2006 2 Puromyc1n technology for proteome exploration using cell free translation Systems Miyamoto Sato E and Yanagawa H Recent Devel Nucleic Acids Res 2 163 173 2006 3 1
31. mRNA PCR DEPC diethylpyrocarbonate 2 PCR 6 PCR 3 TIVV OV en
32. 20gm 2mm 2 800 X700X800 mm AC 100V 24 6 5ml 5 IVV IVV
33. 3 3 3 IVV 2 3 4 1 100
34. b b 1 Mi11iQ Mil1iQ b 2
35. cDNA p53 C 1mM 200ul 1 1 5 4 5 RT PCR DNA p53 C PURESYSTEM S S p5 3 2
36. ee ee ee 24 ee 1 3 4 1 1 600X750X970 mm 2 AC 100V 3 4 1 6 24 6 5ml amp 6 0 huk dal i HE 10 20 30 mm 20mm
37. mRNA RT PCR WH VL cDNA cDNA mRNA mRNA 9 PEG0 2 2 000 RNA mRNA PEPG IVV IVV Sephadex G 200 IVV RT PCR PCR cDNA
38. IVV mRNA 4 5 4 6 NH Hy lgEG cDNA jm vitro wirus 5
39. 1 2 1000 3 8 2 PCR 5 IVV IVV
40. IVV DD 1 7m vitro virus IVV 1 2
41. vitro virus 2 8 2005 2 77 ovirus 28 2005 3 28 2005 4 Jn vitro virus IVV 2005 5 ztro virus IVV 21 2005 1 Tabata N and Yanagawa H Rapid 77 vitro selection and screen1ng of mouse sngle chain antibodies IBC s Antibody Engineering SanDiego 2005 2 Rapid selection of mouse s1ngle chain antibody with high affinity and selectivity by 77 V
42. b b 1 b 2 b 3 Sephadex G 25 b 4
43. vitro virus IVV IVV MAH 1 2 DNA RNA 2 1 IVV
44. BIACORE IVV CDR i IVV MPAA 6 1 2005 198651 2005
45. UV 1 24 4 1 mRNA PCR DEPC mRNA PCR en 2 PCR Q
46. 2 1IVV DNA RNA 1 5ml1 2 IVV RNA DNA 2 3 1 IVV IVV
47. 3 IVV 6 PCR 2 IVV 7999 SO IVV
48. IVV mRNA 4 5 4 6 1 0 0 1 00 1 0 0 IVV
49. 1 2 QIAGEN QIAGEN 6 7 1 0 2 0 3
50. a TT RNase free b DTT DTT 1 DTT 1 Reacrion Mix 1Mix 0 5 1 0 mM 2 10 mM 3 4 1 Sawasaki T 0gasawara T Morishita R and Endo Y 2002 Proc Natl Acad Sci USA 99 14652 14657 2 Madin K Sawasaki T 0gasawara T and Endo Y 2002 Proc Natl Acad Sci USA 97 Ho9 b64 4 4 1 a
51. b 2 50gL 1 26C 24 48 3 3 D O DTT Reaction Mix 50 nL Buffer 1 Mix 1 mL 1
52. 1 2 2 6 2006 7500 04 2 7 2010 9500 5
53. 0 157 SARS 5 1 1
54. PEG mRNA mRNA PEG PCR 2 6 24 6 IVV
55. cDNA DNA 2 4 PCR IVV
56. 7 3 8
57. IVV 3 3683902 2005 IVV 4 WO02003 106675 2003 IVV 5 WO02005 035751 2005 IVV 6
58. IVV IVV 2 IVV cDNA
59. 90 g mL 4 HyHEL10 Vr V 1 150g g mL refolding 5 HyHEL10 Vi Vj CD3 CD3 T ScFv WGE OD40 mRNA 1 mg mL 26 C 20 h TALON resin 0 5 mM 4 mM kDa M1 23456 97 1 celHfree 1 pl nn 2 celLfree 2 pl 66 aw 3 cel free 4 4l Creatine Kinase a t_i i 0 10 20 40 60 81 0 ng A 1 hg1 42 wa i wp ote tit OR WR 5 refolding 40 2 ud 6 refolding 0 4 ng SU HyHEL10 scFv 22 gt 14 4
60. 6 24 6 eg ee Windows 0S 2 DNA RNA RE 24
61. 1080 100 mRNA mRNA jn vitro virus IVV FEBS Lett 1997 mRNA 3 mRNA RNAA jn vztro PCR p53 MDM2 IVV
62. 2 4 1 3 EO 2 PCR QIAGEN QIAquick PCR Purification Kit QIAquick DNA ul
63. 2 WO 2005 083104 A1 2005 3 WO 2005 123936 A1 2005 7 3 8 2 0 0 0 1 1 4 4 i 1 1 0 MM 2 3 1 Misawa S et al CHI PEGS Difficult to Express Proteins May 16 17 2005 Boston MA http www zoegene co jp en Reference PEGS_poster_ZOEGENE Mi sawa pdf 2 Misawa S et al CHI PepTalk Protein Expression January 10 11 2006 San Diego CA http www zoegene co jp Reference 22 PepTalk poster pdf 3 Koga H et al Antibody Engineering Conference June 16 17 2006 Kagoshima Japan http www zoegene co jp Reference 24 posters pdf 4 Eguchi C et al Antibody Engineering Conference June 16 17 2006 Kagoshima Japan http www zoegene co jp Reference 25_posters pdf 1 Misawa S CHI Pep
64. ph CV ER WT 2 2 2 5 2005 1 5 7 1 2 np TL 1 1 vztro virus LIVV jn vitro virus IVV
65. HyHEL10 Vi V WO 80 GOO 400 200 00 0 2 3 4 5 HyHEL10 Wi V 5 HyHEL10
66. 1 0 3 4 0 5 1 2 DA 3 1 28 2005 2 4 EXPO 2005 5 17 19 1 3 BioJapan 2005 2005 9 7 9 1 Development of microarrays 1mmob1lized affinity tagged single chain Fv Kawahashi Y Doi N Kojoh K Tabata N Tsuda C Matsumura N and Yanagawa H 20 TUBMB Internat1onal Congress of Biochemistry and Molecular Biology and llth FAOBMB Congress Kyoto June 2006 4 i D Wh 5
67. IVV 3 IVV 6 PCR 2 cDNA DNA
68. 4 Athena 05 06 29 9 25 25 Hoffmann La Roche Eli Lilly Wyeth MerckSerono MedImmune AstraZenecca Pfizer 10 259 4 3
69. 3 IVV CNG 3
70. b 6 80C 3 2 a mRNA a pEU3b 1 GFP DHFR a 2 GFP DHFR SP6 RNA Promega nmRNA mRNA RNase free mRNA b b 1 2 260 nm 400D 0 4 g nL mRNA mRNA
71. 0 3 3 1 IVV IVV OD 2
72. DHFR GFP GFP 4 GFP DHFR GFP 2 mg mL DHFR 1 5 mg mL GPP mRNA 4 3 IVV
73. IVV 6 2006 799 2016 2500 06 313 12
74. V 1 IVV 1 2 3 zn vitro virus VV
75. 4 IVV 4 5 HyHEL10 HyHEL10 HyHEL10 VV 0 5 mM 4 mM
76. 1 We 2 1 0 1 3 1 4 ln vitro evolution of single chain antibodies using mRNA display Fukuda I Kojoh K Tabata N Doi N Takashima H Miyamoto Sato E Yanagawa H Nucleic Acids Res 34 e127 2006 5 W e b 1 4 EXPO 2005 5 17 19 1 2 BioJapan 2005 2005 9 7 9 2 1 1 Jn vitro virus IVV 1 IVV
77. IAGEN QIAquick PCR Purification Kit 3 IVV 1000 cDNA p5 3 10ng p5 3
78. Talk Protein Process Development January 12 13 2006 San Diego CA http www zoegene co jp Reference 23_PepTalk_presentation pdf 1 126 2007 3 29 http nenkai pharm or jp 126 program pdf 07 pdf Cc 4 2006 288320 1 5 IV IVV a re a ee ee ee pe i IVV
79. itrovirus method Tabata N Sakuma Y Honda Y On1imaru M Matsunaga T Takashima H Miyamoto Sato E and Yanagawa H 20 IUBMB International Congress of Biochemistry and Molecular Biology and llth FAOBMB Congress Kyoto June 2006 3 Development of microarrayS 1mmobilized affinity tagged s1ngle chain Fv Kawahashi Y Doi N Kojoh K Tabata N Tsuda C Matsumura N and Yanagawa H 20 TUBMB Internat1onal Congress of Biochemistry and Molecular Biology and llth FAOBMB Congress Kyoto June 2006 4 Construction of human naive combinatorial library for 7n vitro selaction of single chain Fv antibodies Koiso T Fukuda I Onimaru M Tabata N Doi N Takashima H and Yanagawa H 20 TUBMB International Congress of Biochemistry and Molecular Biology and llth FAOBMB Congress Kyoto June 2006 4 1 5 P 2 2 S 1
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