Cellufine MAX Sr, Cellufine MAX Sh
Contents
1. 1 2 0mg m GH 25 pH 0 01 0 05 M pH 4 to 6 0 1 2 0 M NaCI 1 2M NaCI 5 5 pH 5 0 2M NaOH 1 62. NaOH 20 0 2M NaOH 909 2 NaCl NH4 2SO4 etc pH2to pH14 MAX S r pH3 to pH14 MAX S h at 40 C 1week IN035 036 Ver 3 1 2015 01 22 Celluline AAX JNC CORPORATION Cleaning in place CIP MAX S 0 5M NaOH 300 MAX S 1 000cmh in a 2 2cm diameter column with 20cm bed height at lt 0 3Mpa Over 500cm h 30cm diameter column with 20cm bed height at lt 0 3Mpa pH 2 14 MAX S r pH 3 14 MAX S h 2 209 2 C 89C 1 Harris E L V and Angal S Protein Purification Methods A practical Approach New
3. 6 IN03S7036 Ver 3 1 2015701722 Celluline AAX JNC CORPORATION 7 8 1 000cm h 0 3Mpa 10 9 10 11
4. 10 1 a m cm x cm b x1 2 1 1 c 2 3 40 6096 VV 1 4 1 5 5 a b
5. 20 c 0 5 1 c m 6 7 8 1 000cm h 0 3Mpa 10 9 10 11 10 appendix 1 IN03S7036 Ver 3 1 2015701722 Celluline AAX JNC CORPORATI
6. 1 2 5 1 2 V V or 1M NaCl 30cm h 1 ml c m2 UV Absorbance 50 Volume or Tim e L cm orm HETP N Ve 10 a b N 5 54 x Ve Wh lt As b a Ve Wh a b N HETP As 1 As 0 8 1 6 IN03S7036 Ver 3 1 2015701722
7. Celluline AAX JNC CORPORATION MAX Cellufine MAX S r Cellufine MAX S h MAX MAX S MAX MAX MAX S r MAX S h 2 Characteristics of Cellufine MAX Type S r S h lon exchange type Strong cation SO3 Matrix Highly cross linked cellulose with dextran scaffold Particle size ca 40 130 um lon exchange capacity 0 09 0 21m eq ml 0 10 0 22m eq ml Flow velocity 600 cm h 0 3Mpa 1 D 30 cm L 20 cm pure water at 24 C Dynamic binding capacity gt 130mg IgG ml gel gt 180mdg IgG ml gel pH stability 40 C 1week 2 14 3 14 Chemical stability Stable all commonly used aqueous buffers 1M NaOH Storade 20 ethan
8. York Oxford University Press 1989 2 Janson J C and Ryden L Protein Purification Principles High Resolution Methods and Applications 2 ed New York John Wiley amp Sons Inc 1998 MAX Catalogue No Pack Size Media type MC 1ml x 5 MC 5ml x 5 100ml Cellufine MAX S r 20300 51 20300 55 20300 20301 20302 20303 Cellufine MAX S h 20400 51 20400 55 20400 20401 20402 20403 MC Mini Column JNC 100 8105 2 1 03 3243 6150 FAX 03 3243 6219 URL http www jnc corp co jp fine jp cellufine e mail cellufine jnc corp co jp IN035 036 Ver 3 1 2015 01 22 Celluline MAx JNC CORPORATION Appendix N HETP As
9. ON MAX 0 1M NaCI pH 4 9 pH pH NaC Table 10 DBC vs pH and NaCl concentration Cellufine MAX S r pH Cellufine MAX S h 10 DBC of polyclonal 8 NaCl mM in 10mM NaCl mM in 10mM acetate buffer oO acetate buffer Cellufine MAX S r Cellufine MAX S h 10 DBC of Lysozyme mg mL 9 5 10 DBC of Lysozyme mg mL NaCl mM in 50mM NaCl mM in 50mM Tris HCI buffer Tris HCI buffer
10. ol 1 a ml cm x cm b x1 1 c 2 3 40 6096 VV 1 4 1 5 5 a b 209 c 0 5 1 cm
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