博士論文
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1. 25 14 320 umol photons m sec 20C 10 1 20 L 0 7 mM K2SO4 0 1 mM KCI 0 1 mM KHPO4 2 0 mM Ca NO3 2 0 5 mM MgSO4 10 pM HsBO3 0 5 pM MnSO4 0 2 pM CuSO4 0 5 nM ZnSO4 0 05 nM Na MoO4 0 1 mM Fe EDTA pH 1 M HCI 3 3 2 Fe EDTA2. Ramesh er g7 2003 Zn OsZIP1 OsZIP3 ZRT1 Zn ZRT2 Zn Eide 1998 Guerinot 2000 1 4 Strategy I Fe Fe 2 Fe Fe 3 OsIRT1 Zn OsZIP4 4 12 2 Fe Hshimaru et al Plant J 2006 in press 2 1 Fe MAs Strategy I
3. DNA QIAGEN plasmid kit QIAGEN 1 6 hm 99 5 60 mgmL 2 1 10 1 ng DNA 10 uL 2 5 M CaCb 4 uL 0 1M 3 20 70 10 uu 99 5 99 5 70
4. Zn Zn Zn Zn Maser et al 2001 15 ZIP Guerinot 2000 Eide er al 1996 Korshunov er al 1999 Vert et al 2001 2002 Connolly et al 2002 ZIP 3 4
5. Positron Emitting Tracer Imaging System PETIS Fe DMA Fe Fe Strategy II Fe FRO2 OsFROI OsFRO2 13 Fe Fe Fe Fe Fe DMA Fe Fe Fe 14 2 2
6. Rogers et g 2000 ZIP1 ZIP3 ZIP4 Zn 4z777 4zrt2 Grotz er al 1998 Guerinot 2000 ZIP1 ZIP3 Zn Zn ZIP4 Zn Grotz et al 1998 Guerinot 2000 ZIP2 ZIP4 Cu ZIP4 Cu Wintz er g 2003 ZIP ZIP Zn 11 GmZIP1 Zn 4zrr1 4zz72 Zn Fe Mn Moreau ez al 2002 GmZIP1
7. 180 2 y 11 keV PETIS 1 y 143 mm x 216 mm 2 4 mm Fe 8 3 PETIS Fe 1 mL Fe 2O Fe 30C 65
8. Fe OH 3 Guerinot and Yi 1994 Fe Fe Strategy I StrategyII 2 2 R6mheld and Marschner 1986 1 Strategy I pH Fe Fe Fe Fe Fe Fe FRO2 FROI FRO3 Robinson et g 1997 1999 Fe IRTI Eide ef al 1996 IRT2 Vert et al 2001 Fe FRO2 Fe IRT1 Robinson er al 1999 Waters et al 2002 Bauer ez al 2004 Strategy I MAs
9. 13 1 4 4 1 4 2 4 3 4 4 5 5 2 Fe Zn Fe Fe Zn 0sZ P4 Fe Zn BPDS DMA EDTA FRO GUS GFP IRT NA NAS NAAT MS PETIS ZIP ZRT bathophenanthroline disulphonic acid 2 ethylenediamine N N N N tetraacetic acid Fe Fe chelate reducase B B glucronidase Green Fluorescent Protein Fe Iron Regulated Transporter micotianamine nicoti
10. RNA 1 hg OsIRTI OsIRT2 Os7X77 OsIRT2 Os7A77 OsIRT1RT F OsIRT1RT R Os7A72 OsIRT2RT F OsIRT2RT R ga tubulin ag tubulinm Ose tubulinRTF Ose tubulnRT R PCR 1 yeast extract 2 peptone 2 glucose YPD synthetic defined SD 2 Sherman 1991 Fe Fe SD
11. Fe Fe Fe Morikawa et al 2004 FRO2 Fe Fe OsIRT1 Fe Fe Fe Fe 32 Srategy I DMA DMA Takagi 1976 Mori et al 1991 Higuchi et al 2001 OsIRT1 Fe DMA Fe DMA
12. 2 3i OsIRT1 OsIRT2 OsIRT1 OsIRT2 26 2 9 6 5 777 OsIRTI Os7A77 0 8 kb COS OsyX77 GUS 7 T Fe 2 4 1 a 2 4 1 b 2 4 1 c Fe 2 4 2 a b 2 4 3 a d 2 4 2a
13. 0 5 1 0 cm GUS 4 40C 10 4C 10 992 DTK 1000 microslicer Dosaka EM Co Ltd Kyoto Japan 21 DTK 1000 microslicer 80 um
14. Os7 77 OsIRT1pF OsIRT1pR 0 8 kb PCR DNA PCR Zzzg Xba I pBluescript II SK Stratagene DSQ 2000L DNA Shimadzu Japan pIG121Hm Hiei er al 1994 GUS wdA indI Xba 1 pIG121Hm OsIRT1pro 0 Hiei er al 1994 Oryza sativa L cv Tsukinohikari 4 30
15. Yoshida et al 1981 Kyuma 2003 OsZIP 33 2 4 2 0sIRT1 Fe Fe Os7A77 GUS Os7RTI 2 4 5 Os7A77 7 77 7RT2 Vert et al 2001 2002 Os X77 Fe OsIRT1
16. OsIRTIRTF OsIRTIRTR OsIRT2RTF OsIRT2RTR RT PCR Fe C Zn Mn Cu Fe C Zn Mn Cn OsIRTI OsIRT2 CQ tubulin 2 1 2 Os7RTI OsIRT2 OsIRTI OsIRT2 RT PCR 30 Fe Fe C Fe Zn Zn Mn Mn Cu Cu D600 0 1 0 01 0 001 0 0001 pYH23 OsIRT1 OsIRT2 2 2 Fe OsIRT1 OsIRT2 Fe pYH23 OsIRT1 OsIRT2 Fe 30 pM BPDS SD 2 3 OsIRT1 GFP OsIRT2 GFP a c e amp 1 GFP b f d h GFP a d OsIRT1 GFP e h OsIRT2 GFP
17. 2 4 2 5 777 Os R72 Fe Fe RI PCR Os7RTI OsIRT2 Fe Os7RTI Os7RT2 2 1 77 RT2 7X77 7RT2 Vert et al 2001 2002 IRT1 Fe Zn Mn IRT2 Fe Zn Korshunova et al 1999 Vert et al 2001 Lycopersicon esculentum LeIRT1 LeIRT2 Fe Zn Mn Cu Eckhartdt er al 2001 IRT OsIRT1 OsIRT2 Fe Zn Mn Cu OsIRT1 OsIRT2 Fe ZIP OsZIP1 OsZIP3 OsZIP4 Zn Fe Mn Ramesh et al 2003 4
18. Os7A77 OsIRT2 RT PCR 2 1 2 2 1 Os7A77 Os7RTI Bughio er g7 2002 Fe Os7A72 Fe Os7X77 17 5 2 1 2 3 4 OsIRT1 0OsIRT2 Fe Mult copper oxidase FET3 Fe FET4 Fe CM FET 27 4 et3 4fet4 OsIRT1 OsIRT2 Fe Fe Fe pYH23 OsIRTI OsIRT2 2 2 OsIRT1 OsIRT2 OsIRT1 OsIRT2 Cu Zn
19. OsIRT1 Fe Os7RTI 2 4 5 OsIRT1 Fe Fe Os X77 NA OsW4Ss Inoue et al 2003 Fe OsN4S OsIRT1 Fe OsNASs NA OsIRT1 Fe NA Fe NA 34 Fe Fe OsIRT1 Fe Fe Fe
20. Fe Fe Fe DMA 2 7 a Fe DMA Fe Fe DMA Fe Fe Fe 2 7 b Fe Fe Fe DMA Fe Bio imaging Analyzer System BAS Fe Fe Fe DMA 2 7c Fe Fe 2 7 g Fe DMA DMA Fe
21. 100 um 130 um 6 7 4 6 GUS Jefferson e al 1987 Kosugi et g 1991 1000 rpm GUS 500 pL 30 GUS 1 mg mL 5 bromo 4 chloro 3 indolyl B D glucronide XX gluc 3 mM K4 Fe CN 0 5 mM K3 Fe CN 50 mM Sodium phosphate buffer pH 7 0 20 methanol 37 C 20 GUS 70 700 pL 70 70
22. 2 4 2b 2 4 3 a d 2 4 3 a b Fe RT PCR Osy7A77 GUS Osy A77 2 4 2 c 2 3 5 7777 RT PCR Osy7Z77 2 2 Os7RTI Fe 10 1 29 mRNA Os7RTIl GUS Fe Fe
23. Fe Fe pH Fe Fe Fe Fe 3 1 pH Fe Fe Fe Fe Zn
24. Ga NF TA53 eDS Fe MAs YS7 Curie et al 2001 Schaaf et al 2004 YS7 18 Os7S7 Os7S72 Fe NA Mn NA NA Koike ez al 2004 Strategy Fe MAs DMA Fe Takagi 1976 Fe Fe Fe MAS Fe Fe Fe Os A77 Bughio er g 2002 OsIRT1
25. Fe JIRT1 Fe Zn ZIP OsIRT1I 8 3 4 IRT1 Eng et al 1998 Guerinot 2000 MacDiarmid et al 2000 Rogers et al 2000 Strategy I Strategy Fe Fe 1 3 Zn Zn 10 Zn Zn Fe
26. Fe Takagi 1976 Takagi er al 1984 MAs Mori and Nishizawa 1987 Kawai et al 1988 Shojima er al 1990 Ma and Nomoto 1993 Ma et al 1999 Hordeum vulgare Herbik et al 1999 Higuchi er al 1999 Kobayashi et al 2001 Nakanishi er al 2000 Takahashi et al 1999 200 O ryza sativa Zeg mays Hiiguchi ez al 2001 Mizuno ef al 2003 Kobayashi er al 2005 2005 Fe MAs Fe MASsS Fe MAs SVIN SVIN II AseleDS 2 A391eD1S SVIN II ASe eDS 30O
27. Fe qd c e Fe BAS e s Fe Fe Fe
28. a c 100 nm b d 50 um Fe 2 5 Os A77 a c b a c d 800 um b 30 um a OsFRO1 RAKVDIISAS CRPCGTVELV FSKPASLRYN ALSFIFVQVR E OsFRO2 RVRVRLVSAR LLACDAVELN FCKSPHLTHS PMSTVFINVP CVSRL FRO2 RENVRLLAAR ILPSDTMELT FSKNSKLVYS PTSIMFVNIP SISKL OsFRO1 YHMSILIKVL GSWTEKLRGI ITDAQEQGRN GSESETGRIT OsFRO2 ITMTSSSSLEP DRLSVVVKRA GRWTEKLYET ISSL PPSQPGHLD FRO2 TSSSKLEP EKLSIVIKKE GKWSTKLHQR LSS SDQIDRLA OsFKRO1 OsFRO2 VS FRO2 V E SPYH LMYEN LILVAGGIGI SPFLAILSDI IHRIEEGKPC TPASFLQYDS LVMISGGGGI TPFISVIREL VHRSGTAAEA A S ADFLRHEA LVMVCGGSGI TPFISVIRDL IATSQKET b Cn Fe Mn CU C Zn Fe NE Mn Cu OsFROI 9 OsFRO2 0 Se i rRNAs 2 6 1 OsFRO1 OsFRO2 a OsFRO1 OsFRO2 FRO2 FAD NADPH b OsFRO1I Os7AO2 C Fe Zn Zn Fe Fe Mn Mn Cu Cu a b 40 nmol Fe I g FW h Ja SPAD 35 40 30 35 gt lt 3
29. Fe Fe 1 Zn Mn Cu 2 2 3 1 6 C 15 30 50 mg 11 M HNO32 mL 150 SPS1200VR Seiko Tokyo Japan Fe 238 204 nm Zn 213 856 nm Mn 293 930 nm Cu 324 754 nm s 7 Syngenta rice genome database http wwwr tmri org EN partnership access aspx OsyX77 OyA72 OsIRT2RTL F OsIRT2RTLR cDNA Bughio er g7 2002 PCR Fe cDNA cDNA 10
30. Zn Zn Zn pH Zn Zn Fe Fe Zn Fe Zn Fe Zn 1 2 Fe Fe Fe
31. 1 9 2 SDS RNA 20 ug RNA 0 66 M 1 2 wy Hybond N Amersham USA OsFROI OsFRO2 cDNA P OsFROI OsFRO1 5 OsFRO1 3 OsFRO2 OsFRO2 5 OsFRO2 3 Fe Fe 8 27 10 mmx10mmx1 5mm Cr 99 9 Goodfellow Metals Ltd TIARA Takasaki Ion Accelerators for Advanced Radiation Application AVF 80 MeV a WCr ou 4n Fe Watanabe et al 2001 4 nA 3 2 MBq 143 fmol
32. 3 B 20 30mgL 2 50 mg L 2 30 g L 30 g L 2 g L 5 mM 2 N 2mg Le 1 mg L 250 mg L 30 mg 7L B Murashige amp Skoog MS Murashige and Skoog 196 2 30 g L 250 mg L 50 mgL B MS 28 16 8 L 7 7 TT GUS Ti MS 2 C 14 320 umol photons m sec 20C 10 2 20 L 2 Fe EDTA Fe 1
33. 3 Fe Fe 7 1 0 9 550 110 16 71 7 14 38 9 8 6 1 8 13 1 7 8 1 9 13 2 X 105 copies ug RNA 2 2 DOxsR77OD RTPCR Os 77 RT PCR RNA 1 Lg Os 7A77 3 a elR11 AB070226 AIRT2 OsIRT2 T04324 AB126086 AtIRT1 U27590 OsZIP4 AB126089 OsZIP1 OsZIP3 AY323915 AY30258 OsZIP2 ZIP1 AY30259 AAC24197 OsZIP6 OsZ OsZIP7 AB126088 AB1 26087 AB126090 02 b OsIRTI OsIRT2 gt 5704 9689 10574 OsIRT2RTF OsIRT2RT R OsIRTIRTF OsIRTIRT R 2 1 1 Os7RTI OsIRT2 a ZIP Os Oryza sativa Le Lycopersicon esculentum At Arabidopsis thaliana b Os A77 OsA72 Os7A77 1 3 Os7RTI OsIRT2
34. 99 5 28 inch Hg flight distance 1 6 cm 3 target distance 6cm 1330 psi 1100 psi 3 26C 14 10 23 16 8 19 LSMS10 Carl Zeiss 1 cm 488nm pm
35. 2 4 3 Fe PETIS Fe Fe Fe DMA Fe 2 7 Fe Fe Fe3 DMA Fe NA 447 4 7yW447 p DMA Takahashi er g 2001 DMA Fe 39 OsIRTI OsIRT2 OsIRTI OsIRT2 Fe 12 1 2 1 0 3 6 6 0 7 1 0 0 1 Fe 460 60 26 1 1 1 0 1 0 10 0 10 x 106 copies ug RNA 2 1 Os7RTI OsIRT2 RT PCR OyA77 OsIRT2 RT PCR RNA 1 ug Os7 77 OsIRT2
36. 1950 8 The State of Food Insecurity in the World 2001 Fe Zn
37. OsFRO2 Fe Fe Fe 2 6 2 a Fe Fe SPAD Fe 2 6 2 b Fe 30 Fe 2 3 9 Fe Fe DMA Fe Fe DMA PETIS Fe Fe Fe Fe 0 43 MBq 30 7 fmol Fe 1 12 umol 0 1 mM Fe DMA Fe DMA 0 43 MBq 30 7 fmol Fe DMA 1 12 nmol Fe
38. GFP j a e 1 20 Llm b c d f g h 10 um 2 4 1 Fe Os 77 a b c MXII CC EN Pr PP a 400 um b 100 pm c 50 Lum nt EE 00 10 20 30 40 30 60 70 8 0 x 10 copies 15 ng RNA 2 4 2 Fe Os7A77 a b a 400 wm b 100 jim c Fe RT PCR RNA1S ns Os7A77 3 2 4 3 Fe Os A77 a b a c d c MXII CC EN Pr PP LR
39. 17 Fe Cr Fe Watanabe er g7 2001 Fe pH 0 1 1 N KOH pH 3 9 8 mM DMA 1 Fe DMA Fe 0 1 mM 2 24 PETIS PETIS Positron Emitting Tracer Imaging System PET ee Emission Tomography CN e n v e 0 6 1 7 MeV
40. 2 Fe 2 3 8 Fe Fe Fe Fe FRO2 2 FRO2 OsFROI accession number AB126084 OsFRO2 accession number AB126085 2 6 1 a OsFRO1 OsFRO2 FRO2 30 4 48 2 FRO2 FAD NADPH 2 HPFT GPYG OsFRO1 HPFT T S OsFRO2 GPYG G S 2 6 1a OsFROI OsFRO2 2 6 1 b OsFRO1 Zn Mn Cu Fe
41. 31 2 4 2 4 1 0s1RT1 Fe OsIRT1 OsIRT2 OsIRT1 Fe Os7A77 OsIRT2 Fe 2 1 2 2 1 OsIRT1 OsIRT2 Fe Fe 2 2 OsIRT1 OsIRT2 GFP 2 3 Os7RTI 2 4 PETIS Fe 2 7 Fe Fe FRO 2 6 Srrategy I Strategy I Fe Fe OsIRT1 OsIRT2
42. 70 2 3 Axiophoto microscope Carl Zeiss Tokyo Japan Fe 1 cm 3 1 4C 15 4 C 1 22 16 um LMD Leica AS LMD Nakada er g7 2005 RNeasy micro kit Qiagen RNA RNA T7 RNA polymerase based RNA amplification Nakazono et al 2002 RNA super
43. Fe Fe 50 uM BPDS pYH23 Bughio er g 2002 17 pYH23 4DHI HindII Pvull Pstl XhoI Sst TI 42g Not1 pYH23 7o 1 AozI OsIRTIcDNA Ao 1 Not I Os7A77 pYH23 Osy7A77 Bughio ez g 2002 Os7RT2 lithium acetate Gietz and Schiest 1995 SD 3 mL 2 300 pL SD 3 mL 6 SD OD 0 1 10 UL 10 50 uM BPDS SD 0sIRT1 GFP 0sIRT2 GFP OsIRT1 GFP
44. 250 pmol photon m s 1 PETIS 3 PETIS 6 1 Fe DMA 0 43 MBq 30 7 fmol Fe DMA 1 12 pmol 2 Fe 0 43 MBq 30 7 fmol Fe 1 12 umol 0 1 mM 3 Fe 0 43 MBq 30 7 fmol Fe 1 12 umol 26 2 3 2 3 1 s 7 Fe cDNA Os7X72 cDNA accession number AB126086 OsIRT2 OsIRT1 73 2 1 1 a Osy7X77 OsIRT2 3 2 1 1 b Os7A72 cDNA 5 Oryza sativa L ssp Japonica cv Nipponbare Goff er al 2002 2 1 1b 2 3 3 s 7 77 7 OsyA77 Os7X72
45. Fe Oryza sativa Strategy IL Fe Fe Os7RT7 Buguho er g 2002 Strategy I Fe Fe OsyA77 Oy A72 RT PCR Os7RTI OsIRT2 Fe Fe Os X77 Os X72 Fe OsIRT1 OsIRT2 GFP 4ium cepg L Os X77 GUS Os7RTI Fe
46. OsIRT2 GFP CaMV35SS sGFP S6ST NOS3 CaMV3SS sGFP S6ST NOS3 35S 3 Sg7 1 AcoI Aco I CCATGG SGFP Sal I GTCGAC Kpnl GGTACC SI Ncol Adaptor GFPS Adaptor GFP3 CaMV3SS Sg7 KpzI sGFP S6ST NOS3 Fe cDNA OsIRTI ORF OsIRT1GF OsIRTIGR OsZRT2 ORF OsIRT2GF OsIRT2GR Os7RTI OsIRT2 Sall Kpnl 16 CaMV33S Sg I KpzI sGFP S6ST NOS3 Bio Rad Biolistc PDS 1000 He
47. script H Roche cDNA cDNA Amplitaq Gold Applied Biosystems RT PCR RT PCR 94C 10 98 10 50C 30 72C 1 30 RI PCR Fe Fe 50 mL 0 2 mM CaSO4 5 mM HEPES pH 5 5 0 1 mM Fe EDTA 0 2 mM BPDS 25C 1 535 nm Fe Fe 3 SPAD 502 Minolta Co Tokyo Japan Fe
48. 0 2S 20 1S 6 10 10 5 0 1 3 S T 1014 4 3 lt 7 1014 Ti 13 2 6 2 Fe a Fe 3 Fe Fe 0 1 3 5 7 10 14 Fe 1 3 3 b Fe a SPAD S02 3 3 b MBq7g FW 100 2 3 Fe 2 0 Fe DMA 80 _ 1 5 5 60 1 0 40 0 5 20 0 60 120 180 240 300 360 min 8 relative Count LL L 4 J 0 60 120 180 240 300 360 min 2 7 PETIS Fe Fe DMA Fe a Fe Fe DMA Fe b 6 Fe 3 c Fe DMA BAS
49. GCGGCCGOCTCACGCCCACTTGGCCATGACG OsZIPI forward 5 CACCGGCGCAAGCTTCGACCATGGCCAGGA OsZIPI reverse 5 GAAGCAAGTCTAGAACTAGGATGGATGGATOC OsZIP3 forward 5 CACCATGGGAGCCAAGAAGCATACCTTGCA QOsZIPS reverse CTATGCCCATATGGCAAGCATTGACATCAO OsZIP4 RT F 5 GCOGAAAGCAACAGTGATCATGGCGACTTTO OsZIP4 RT R 5 GCAGCTCTTGGTTGCTCTGAAGATCTCATO OsZIPI RT F 5 CTCTTCAAGTTCCTCGCCGTCCT OsZIPI RT R 5 CGGCCACGATTAATGAATGGGGT OsZIP3 RT F 5 AATGTGCATAGCTCAACTGCCTT QsZIP3 RT R 5 CAAAATCAAGCCTATCTGGGA OsZIP4 ORFS 5 CACCATGGACGCCATGAGGCAGAGCACGCG OsZIP4 ORF3 5S TGCCCATATGGCAAGCAGAGACATCATCCC CM3260 parent strain MATalpha trpl 63 leu2 3 112 gen4 101 his3 609 ura3 52 CM FET Fe MA4Talphatrp1 63 leu2 3 112 gcn4 101 his3 609 ura3 52 Afet4 TRP Afet3 HIS CM SMF Mn M4Talpha trp1 63 leu2 3 112 gcn4 101 his3 609 ura3 52 Asmfl URA3 CM ZRT Zn MA4Talpha trp1 63 leu2 3 112 gen4 101 his3 609 ura3 52 Azrtl URA3 Azrt2 HIS3 FTRUNB1 Cu M4Talpha trp1 63 leu2 3 112 gen4 101 his3 609 ura3 52 Actrl URA3 1 1 1 2005 64 7600 2005 2050 25
50. Mn Cu CTR1 Cu dc Dancis et al 1994 Zn ZRT1 ZRT2 Zn CM ZRT 4zz77 4zrt2 Mn SMF1 Mn CM SMF 4sz77 OsIRT1 OsIRT2 OsIRT1 OsIRT2 Cu Zn Mn 2 3 5 OsIRT1 OsIRT2 OsIRT1 OsIRT2 GFP OsIRT1 GFP OsIRT2 GFP 2 3 a h GFP
51. anamine synthase nicotianamine aminotransferase muginetic acid Murashige amp Skoog Positron Emnitting Tracer Imaging System Zinc regulated transporters Iron regulated transporter like Protein Zn Zinc Regulated Transporter OsIRT2RT F 5 CGTTCCACACGCGGGGCAGCAAG OsIRT2RT R 5 GGATGATGATCCGTACGGCAGAAG OsIRTIRT F 5 CGTCTTCTTCTTCTCCACCACGAO OsIRTIRT R 5 GCAGCTGATGATCGAGTCTGACO Ose tubulmRTLF 5S TCTTCCACCCTGAGCAGCTO Osa tubulinRT R 5 AACCTTGGAGACCAGTGCAG Adaptor FPS 5 TCGAGATATCGGTACCAGATCTGAGCTCGAGGTCGA Adaptor GFP3 5 CTAGTCGACCTCGAGCTCAGATCTGGTACCGATATC OsIRKT1GF 5 GTTGGAATTCCGTACGGCATGGCGACGCCGCGGACACTGGT OsIRTIGR 5 ACAACTAATGGCGGCCGCTCACGCCCACTTGGCCATGACG OsIRT2GF 5 CACGCGGTACCCCACTTGGCCATGACGGAO OsIRT2GR 5 GCTGGTACCTAACATCGAAGCTTTGTA OsIRTlp F 5 CGAGGTCGACGGTATCGATAAGCTTTCTTTO OsIRTlp R S CGCTGTGCACTCTAGATTTTGCTACGTACGTACTCAGG OsFRO1 5 5 CAGCCGGCCTGCTAATGTGG OsFRO1 3 5 CCAAGCACCTTGATCAGGATCGACAT OsFRO2 5 5 GGCATIAGAACTCGCCTTCTTTCTO OsFRO2 3 5 CGCGTAGAAGAAGAGCTCGAACATO refrel 372 F 5 GCGCGCGGTACCTCTAGATGGTTAGAACCAGAGTO refrel 372 R 5 CGCGCGCTCGAGCCAAGTAAAACTCTCCTCCTCTA OsZIP4 forward 5 CACCATGGACGCCATGAGGCAGAGCACGCG OsZIP4 reverse 5 TCATGCCCATATGGCAAGCAGAGACATCAT OsIRTIforward 5 CACCGAATITCCGTACGGCATGGCGACGCCGCGGACACTGGT OsIRTI reverse 5 ACAACTAATG
52. cm x 25 cm Luiria Bertani LB 1 25000 50000 6 Fe cDNA 1 3 Os A72cDNA Thermo Sequenase Cycle Sequencing kit Shimadzu Kyoto DNA sequencer DSQ 2000L Shimadzu PCR Fe Fe Zn Cu Mn RNA SDS RNA DNA DNaseI RNase free TaKaRa SuperScriptII Imvitrogen 16 Smart Cycler IaKaRa RT PCR Smart Cycler
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