バラスト水管理システムの承認の際の 生物分析方法（第2回
Contents
1. 1 4 13 2 1 E2. 2 2 L 2 1 100 6 3 36 3 1m S 1 SE 6
3. 66 2 1m
4. 4 4 3 4 4 4 4 4 5 E 4 5 6 4 5 1
5. 5 6 2 2 5 6 2 2 1 5 6 2 1 5 6 2 1
6. 1 51 2 Lg HH Ed 5 4 7
7. 1 3 1 4
8. ni 3 4 4 2
9. 4 1 5 a 5 2 2 b
10. 2 3 2 1 1 2 10
11. eT 4 10 3 EALE EEA EAT ES AN 4 10 4
12. 4 5 2 4 5 3 4 5 4
13. e 4 2 3 4 2 4 ga
14. 2 65 5 10 4 3 5 10 4 4 5 10 5
15. zr Ed FOIZI 8 s E HH HE
16. Ju EAT K SB E RH 1
17. 1 HB 3 53 2 F1 K A d P 1H HA
18. 1 2 1 3 D 2 D 2 2016 D 2
19. 5 4 4 a b c type d e
20. 4 9 HR 5 5 8 59 E 5 5 9 EI E 5 5 10 5 5 11
21. 3 Aic 5 4 6 5 4 6 1 5 4 6 2 ME Isi cross sensitivi
22. EHI E 3 5 9 Pes S zE LB Jg E 63 a
23. 3 TL 3 9 2 f 20um 3 9 22 L 2 Sefar TLXX 35 20 4 RU Ge ORE T OE EE 25 AUR 5 OD E Viet 7e PIC o Cm amp 729 TEX 5 X v7 vi 100mC 10mt 6 3 10
24. 5 10 7 5 4 7 5 10 8 1 2 Y T T 5 10 9
25. 2 5 7 2 62 O 5 7 3 1 E E
26. 5 8 5 8 1 5 8 2 7 1 E
27. 3 5 4 5 3 cross sensitivity 1 2
28. a b c 5 5 2 d e D g h 5 5 6 ih c
29. EE Gd 2 54 Do I 5 3 2 UE EH 5 3 3
30. 5 2 2 H DE 5 2 3 5 2 4 5 2 5 WEST
31. 55 5 4 3
32. 003 2 2 2 10 50 u m e 50 u m 50 um 10 50 u m 1 2 2 2 D
33. 21 4 6 4 1 4 viability 4 1L 4 1 1 1 2 4 1 2 50 um IL 4 1 1 1 2 2
34. 1 1 G8 pum 2 1 Quality Assurance and Quality Control Procedures 2 1 1 The testing body performing the tests should have implemented appropriate quality control measures in accordance with recognized international standards acceptable to the Administration 2 1 2 The approval testing process should contain a rigorous quality control qualityassurance program consisting of 1 Both a Quality Management Plan QMP and a Quality Assurance Project Plan QAPP Guidance on preparation of these plans along with
35. 1 3 D 2 D 2 D 2 2009 2016 D 2 D 2 1 D 2 2 D 2 1 50um L 1m 10 30um 10um S
36. 4 1 4 Q5 A 4 1 3 96 ILX 0 1 1 Q6 ISO17025 ZA w A ISO17025 TI 2 Q 7 QMP 4 5 3 SS
37. 4 6 4 6 1 HH 4 6 2 E output E
38. 5 6 3 2 S I internal 5 6 3 3 5 6 3 4
39. 99 1 1 1 2 1 3 1 4 1 5 6 viability Calcein AM L S Calcein AM C4SH45N2053 994 86 CASNo 148304 34 1 ex 490 nm em 515 nm 1 Working solution Calcein AM solution 200 ul 10 ml 20 umol 2 a Working solution 1 ml b 59405 Pluronic amp 0 3 ml A c 2 ml 4 d 20 C 1 fi amp 35 J OUHOEBLES 2
40. 3 1 m ESES Ar 22 4 1 2 minimum dimension 1 TL4 1 1 4 1 2 T4 1 3 I4 1 1 a b gt BdII 41 2 a b c E a b 23 4 1 5 minimum dime
41. Ei K RUOTE Y Be 4 E 4 1 E E Ez LTOS LYT X0HB81nojxk jt 2 HI 4 1 L Q11 50u m 4
42. 5 7 5 7 1 r ah
43. 7 TXL 5 3 5 3 1 1 E um
44. 2 27 4 2S 4 2 1 1 1 mt 2 II 4 2 1 T 4 2 1 1 E 10 um 50 um 10 um 50 um 30 um VN 3
45. 1 4 12 2 2 4 12 2 3 4 13 4 13 1 E
46. O 5 8 3 1 KERE 1 H Im Es H HH 5 8 4
47. ABS ARE e KA VI TUERDE BORA 72 No
48. 4 10 5 N E E E 4 13 50
49. E A H 2 7
50. 4 11 4 11 1 4 11 1 T 2 EDDA 4 12 4 12 1 4 12
51. I 1 3 D 2 2 3 20 Zzzo cholerae Coliform Enterococcus group D 2 D 2 D 2 D 2 D2 2 toxiogenic Vibrio cholera
52. a que iN Dn E30 56 N 1 2 T
53. Ec 5 4 2 rx EE A USIE s Da AUR XC
54. D 2 6 3 T L 20 Im S 1 108 8 6
55. m Qu 300mt 9 pH DO TSS DOC POC RUWE AF NTU 10 G8 Part4 2 3 3
56. 3 HDXEBUE 60K UTEN b 00 00 pi 77 2e H EE ATUS kir c raf 4 8 1 4 9 4 9 1 a 7 AIR DE rH 3 4 10 1 ll HIE EK XC 3 76 ORRA D 2 0 ff TE E EKRU E CB ka
57. S TRIER Hl A ran Ei od A n nu N ta KE c o 1o014 0M 10 L 1 2 S 3 4 Oo OO O00 OO OO RW Ww Ww NN Ne Od d0007000 nann KR MR Aog MR mo OX t II 1 2 3 4 ISO IEC 17025 1 2 5 6
58. 4 1 3 4 1 4 1 N Iss 2
59. 5 10 3 1 d 5 10 5 e 5 10 3 2 5 10 2 RU 5 10 3 1 a b c d e f RM H m Er
60. 6 7 8 Q 60C 2 TSS TSS mg O Ws Wi B V Wi mg Wo V mg B V DO DOC 1 450C 2 3 0 7z m GF F 0 45wm POC 1
61. type E 4 6 3 3 amp 1 EE Ek grad j XH class E E 4 6 4 AE d 3 E E E e A 1 E 3 1 4 7
62. S I 6 7 BIPM 8 5 6 2 1 2 S I
63. 4 10 4 10 1 S RE F4 E 4 10 4 10 2 i Hr HH
64. 1 5 1 6 JIS Z 9901 1998 p xc cuu UU 9902 1998 JIS Z 9901 1998 JIS Z 9902 1998 g Z 9901 1998 JIS Z 9902 1998 IS0 IEC Guide 21 TIDT MOD NEQ ISO IEC 17025 1999 General requirements for the conop
65. ii A G8 3 V EET DRY IL o 3 9 1 Q10 2 35um 30mt A 4 E S0um mt v MC
66. 4 3 4 3 1 VM 1 mE Md SG fx 46 Z
67. 2 5 6 2 2 2 S 1 5 6 2 1 2 61 5 6 3 5 6 3 1 5 6 2 1
68. 1 v 2 4 2 2 a ERU RREH E b c d 1 US
69. 45 0 5C O O1 O139 cholera enterotoxin Heterotrophic bacteria 20 28 C 3 7 TL 4 1 4 7 4 1 2 4 1 3 18 25 E E 18 Bacteriological analytical manual 8th ed AOAC International Gaithersburg Md 19 Clinical microbiology procedures handbook American Society for
70. G8 IMO G9 MO 2 2 G8 4 Part 1 Part 2 Part 3 Part 4
71. Eme 5 3 4 s JE pis 5 3 5 4 4 5 4 E 5 4 1 4 3 1
72. 5 2 5 3 5 4 5 5 5 6 5 7 5 8 5 1 2 5 2 5 2 1
73. 5 10 5 4 6 3 1 1 2 3 xu HH H II GUM 5 4 7 5 4 7 1 5 4 7 2 E
74. No S L D T Mini ui D dimension 69 No Gg x S L m Minimum 6 70 L S
75. 2 4 G8 QMP QAPP d I3 Z3 2s ug fv Eu En N f 7 NephelometricTurbidity Unit 3 3 1 G8 n AZEUL TOD D 2
76. 3 D 2 D 22 Escherichia coli Intestinal Enterococci Toxiogenic Vibrio cholerae O1 and 0139 G8 2 3 20 Coliform zz FE Enterococcus group Vibrio cholerae X 3 V 718 Heterotrophic bacteria B 8 EKO TITO 8B D
77. E E 60 2 ST VIM 1993 6 4 S I CCPM CIM 3 S SI 4 5
78. 2 22 3 Qc E CO www tO PO OO E EL An 1 2 3 D 1 2 3 4 1 2 3 mi 1 2 d SEXE 2 Ed Wi N Zi 2 3
79. 2 R2A R2A Agar Marine Agar 2216 DSMZ Medium604 R2A Agar WERE XA Yeast Extract 0 5g Acid hydrolysate of casein 0 5g 7 FYH Glucose 0 5g Soluble starch 0 5g KoHPO 0 3g Sodium pyruvate 0 3g Pancreatic digest of casein 0 25g BREQASV Bx Peptic digest of animal tissue 0 25g MgSO4 anhydrous 0 024g Agar 15 0g Purified water 1000m pH7 2 0 2 121 1C 15 Marine Agar 2216 Peptone 5 0g Yeast Extract 1 0g Ferric citrate 0 1g Agar 15 0g Seawater or artificial seawater 1000 mt pH 7 6 0 2 121 1C 15 30 31 30 American Public Health Association 2005 S
80. viability 4 3 6 7 6 9 7 5 85 9 6 100 2004 4 DIE IMO 2 e A 28 E 2 IMO REI D 3 IMO AY 2005 4 MEPC 53
81. MEPC 58 INF 10 2008 aomura ecc mail u tokyo ac jp 101 2 1 2 2 2 3 2 4 2 5 c eg L S GERE 2006 EHE neutral red Cj5H44CIN47228 78 CAS No 553 242 1 Working solution 2 1 w y a 10 ml Working
82. D 53 55 Peptone Yeast extract Sodium citrate Na35S8504 NaCl Ferric citrate Oxgall Sodium cholate Sucrose 10 0g 5 0g 10 0g 10 0g 10 0g 1 0g 5 0g 3 0g 10 0g 53 American Public Health Association 2005 Standard methods for examination of water and wastewater 21th ed American Public Health Association Washington D C 54 U S Food and Drug Administration AOAC International Gaithersburg Md 55 2004 um TCBS 1963 H 18 2000 Bacteriological analytical manual 8th ed 387 391 38 2 0 2 0 2 Thymol Blue 20mt 0 2 0 2 Bromothymol Blue 20mt EK Agar 15 0g Purified water 1000m pH 8 6 0 2 JH TAE LC Hw S 2 AR 4 3 1 3 3 4 3 1 4
83. E E 5 10 4 2 ZEE E S I S I S I SIX E T gt
84. Q19 5 A A G8 10 3 Q20 Minimum Dimension A G8
85. 3 6 pH DO NTU St 3 7 3 6 100mt pH 500m DO 100mEDO DO 3 8 3 6 pH DO NTU T 3 8 1 3 8 1 pH DO NTU 3 9L 391
86. S0um 10um 6 K MEREKA L POC TSS 2 D TRC G8 2 5
87. ISO IEC 17020 JIS Q 0065 1 2 3 5 10 6
88. 5 5 12 1 5 6 WEO F 5 6 1 WR KEX ax ii CO e LEAN IS CO VC FECERIT m MEE E 1 WS 5 6 2 5 6 2 1 5 6 2 1 1 S I H D zu Ef
89. 70 nei 74 4 A EARDERE UO I I0 AT EE EOE S L pag 1 1 KOR m 1 1 1 S 1 1 1 CARIE E IL j Im d 4 ES D D i Bacteriastrum furcatum Bacteriastrum sp Chaetoceros sp Guinardia sp Nauplius Odontella sp Protoperidinium sp Tintinnopsis lobiancor M SABES
90. 4 1 E T ATZREEPE I EIE e ESELC b TL OFRECE U 5 jii COE FH LO O ff 2 A E E
91. 4 Dco ege u 2 P OUS I IL Ba EO EBON 3 18 1 3 EIES f E 7 Rs
92. pH al USO ex uA Opes Rp ex B 5ml gm 200ut 47mm 47mm 02um EE 100 mt 1 2 OD CESTA A EENIA
93. 2 01 O139 1 40 4 4 G8 CX RRIF ORISI 2 7 00183 M 4 4 1
94. D 2 D 2 1 T D 2 1 3 poca eu amc 4 9 5 3 X3 X3 3 L Im S 4 E 1 500m L S
95. i E 49 b c d e 4 9 2 RHI nu 7 j
96. b b c d e 5 10 5 10 1 1 1 1H E E 1
97. Part 4 Resolution MEPC 126 53 Procedure for Approval of Ballast Water Management Systems that Make Use of Active Substances G9 Resolution MEPC 169 57 23 G8 G8 2 3 1
98. a E 97 A L S 3 S E 4 1 1 L ET II 4 1 3 Q12 eic tdi A ZM ri 4 NE E 7e Nitzschia 3 E minimum dimension II 4 1 4 Q13 1 A
99. 6 Q3 SS Somg L A G8 1 3 3 Q4 CEBRZR CX Viable organisms PITER A 4 1 4 viabiliy
100. ATA 52 12 1 1 2 3 4 14 2 5 5 1 5 1 1 NN
101. a 5 4 2 b c 5 4 2 1 E 47 l 2
102. Enterococcus Agar m Enterococcus Agar Azide Agar M Pancreatic digest of casein 15 0g Papaic digest of soybean meal 5 0g Yeast extract 5 0g KH PO 4 0g Glucose 2 0g NaN 0 4g Triphenyltetrazolium chloride 0 1g Agar 10 0g Purified water 1000mt pH 7 2 0 2 Jui TAE LC Hv S 3 4 3 1 3 4 4 3 1 4 4 3 2 5 37 2 48 3 5 T 4 3 5 Ime 6 D 6 5
103. Sefar NY7 HD Super 17 4 RIE ORT O E DRU OD EE Ee PC o Cm amp 72 o TEX er 5 10 mt 1 mt 6 3 4 BEERE LTO HRR 16 T 3 4 1 X E II 3 4 1 3 5 TSS DOC POCO 5e 2 1
104. 2 6 3 pH pH 8 0 pH pH 8 5 pH pH 7 0 ds 2 7 Crippen and Perrier 1974 The use of neutral red and evans blue for live dea
105. HH Ed m initial 4 4 4 4 1 amp DJI
106. 2 MI medium 41 46 41 American Public Health Association 2001 Compendium of methods for the microbiological examination of foods 4th ed American Public Health Association Washington D C 42 American Public Health Association 2005 Standard methods for examination of water and wastewater 21th ed American Public Health Association Washington D C 43 U S Food and Drug Administration 2000 Bacteriological analytical manual 8th ed AOAC International Gaithersburg Md 44 2005 2005 45 2004 46 2001 4L z HH 48 Bernner K P C C Rankin Y R Roybal G N Stelma Jr P V Scarpino and A P Dufour 1993 New medium for simultaneous detection of total coliforms and Escherichia coli in water Applied and Environmental Microbiology 59 3534 3544 49 U S Environmental Protection Agency 2002 Method 1604 Total coliforms and Escherichia coli in water by membrane filtration using a simultaneous detection technique MI medium Publication EPA 821 R 02 024 USEPA Official of Water
107. 5 1 H HX Aii Xe ON joo pier d 201 50 3 58 5 6 5 5 3 o 5 5 4 5 5 5
108. k LH E OB 64 l 2 5 10 3 5 10 2 a b c E FEL BAs
109. 45 0 5 48 100 100 100 T 50 51 50 American Public Health Association 2005 Standard methods for examination of water and wastewater 21th ed American Public Health Association Washington D C 51 U S Environmental Protection Agency 2002 Method 1600 Enterococci in water by membrane filtration using membrane enterococcus indoxyl 8 D glucoside agar mEI Publication EPA 821 R 02 022 USEPA Office of water Office of Science and technology USEPA Washington DC 5 Slanetz L W and C H Bartley 1957 Number of enterococci in water sewage and feces determined by the membrane filter technique with an improved media Journal of Bacteriology 74 591 595 37 II 4 3 6 pH 7
110. G8 A c DOC POC o pA 5 Q8 SS A SS SS Ed d Q9 ik
111. SS I 2 Q17 POC DOC 10 50u m Y ml 10 3 gt IT H Minimum Dimension 10m A 34 E 98 Q18 D 2
112. X BS2 ISO9001 DE 2 1 5 6 L S 2 1 IV efi iX G8
113. m EN PR V 5 4 7 2a 5 5 2 uu ce EX ii D 4 h ME RI 5 o
114. Total E EE E AA L 15 20 Harpacticoida 100um Total Total m F T P F T P F T P No 1 050916 150ml 150m1 S 0509160 RRA ee Fines O No 2 150ml S 050916 150ml No 3 050906 150ml 150m1 S 0509168 16 00 Re pecie HENENEN GENET NN S y pee o WM cor a2 S S 83 cfu ml Marine agar 23 C 6 cfu ml R2A agar 23C 6 05 09 18 05 09 17 D 3 4520 5 080 224 5 760 124 05 09 16 20 950 2086
115. c d e f g NN h
116. 57 5 8 5 9 5 10 4 1 4 14 ISO9001 2000 Er ISO9001 2000 lt ISO9001 2000 4 4 1 4 14 ISO IECI7025 5 1 5 10 ISO IEC17025
117. ISO IEC17023 QMP QAPP QMP ISO IEC17025 4 4 1 4 2 4 3 4 4 4 5 4 6 4 7 4 8 4 9 4 10 EELE 4 11 4 12 4 13 4 14 IH 5 52 5 3 5 4 5 5 5 6
118. b 1 c 1 E Ee d e f g h i j
119. 32 PSU 3 32 PSU 3 PSU 2 5 10 3 2 10 PSU DOC POC TSS L 3 5 10 m S 3 5 10 mf 10 cfu mf 4 3 D 2 D 2 1 10
120. J EOS id WM 5 3 2 L GS 8 15 E E 8 The Handbook of Standard Method For the Analysis of Water and Waste Water 9 ISO standard methods 10 UNESCO standard methods 11 World Health Organization 12 American Society of Testing and Materials ASTM standard methods 13 U S EPA standard methods 14 Research papers published in peer reviewed scientific journals
121. figs Ze LC 100 mt 1t 15 35 mt 1 mt 45 70 mt 3 135 210 mt 4 20 10 um 4 2 2 minimum dimension 1 2 4 2 3 minimum dimension 4 1 3 4 2 4 4 EW viability ES 28 4 1 4 4 2 5 4 1 5 29 4 1 1
122. 0 1 C 1PSU pH 0 1 DO 0 1mg t NTU 0 1 4 4 2 pH DO NTU E JIS EH FHS TSS POC pH DO NTU TSS DOC POC G8 DO
123. 2 86 3 No 10 S S 6 EXER neu 7 17 5 ml S 00 51 2 0 0 0 0 0 0 0 01 37 US Ditylum braightwelli 1 o O Skeletonema costatum 8 Thalassiosira sp 13 051111 Br EA 7 BUE exp 7 173 ml Cochlodinium sp p p E Ditylum braightwellii a Surirella sp 1 y EG uen Hr PE 17 5 ml 1ml 00 30 01 45 m 6 Ditylum braightwellii 2 Planktoniellablanda 1 y Protoperidinium sp 1 y TITTYTTTISTS Westella sp AE 87 4 il z J 1000 ml 27 5 ml 2
124. T O 1 2 H HH 4 EA QAPP E QAPP QAPP D 2 BS BS2 WHO Laboratory
125. 2 PW EAE BH IXEXSIBIEBHXE I Ak BL EP 1 1 1
126. B 5 5 6 ih c 5 5 7 i
127. 20 ml 5 76 50918 ed No 7 DO o olo 0O 1 cu AUtrestomth 9 2 8 25 ml 1 mlX3 S050918 8 TEA EE D GG OQ 1 regrowth 7L 9 27 T 1 2 1 2 1 050920 F TJP 20L qe 10 00 S TESR 12 00 x C EE Protoperisinium sp 50pm 1000 y Coscinodiscus granii 50ym 700 y Coscinodiscus sp 50hm 1000 y a i Scyphozoa polyp 80 1000um 4 o pb y Polychaeta larva 80m 00 3455 d c 0 0 2j Pelecypoda l
128. 1 E 1 400 3 6 3 2 s E 3 G8
129. 1 mt 10 D 2 2 1 O 1 0 139 1 cfu 100m cfu colony forming unit 1 cfu 1g 230 cfu 100 mt 3 100 cfu 100mC PA REIH 4 1 2 4 1 3 4 1 4 2 2 1 2005 7 2008 10 G8 D 2
130. 1 S 10um 40m 3 3 2 10 um 3 3 2 S 2 E y 3 EN II 3 3 3 FA II 3 3 3 S
131. IMO IL Fu amp cd EN 2 A 0 ARA 22 3 CH E es D Y lt ie 19 E AA Hj XE H E International Convention for the Control and Management of Ships Ballast Water and Sediments Resolution MEPC 125 53 Guidlin
132. 25 a T1 EZHh B ok NaNO 1x10 NaH PO 1x10 TFL O OT 3 MWER M 7 Fe EDTA 3HO 5x10 ZnSO4 7H O 1x10 MnCl 4FHO 1 X10 NajMoO 2H O 5x107 CoCL 6H O 2x107 CuSO4 5H O0 1x10 Na EDTA 2H O 2 4 X 10 H5SeO 2x10 Thiamin HCI 5 93 X 107 EG Biotin 4 1 X10 Cyanocobalamin 7 38 X10 mol 6 b 2 NaNO 8 83 X 10 NaH PO 3 63 X 10 Na SiOs 9H 0 1 07 X 10 FeCl 6HzO 1 X10 Na EDTA 2H O 1X107 CuSO4 5H O 4x10 NaaMoO4 2HbO 3x10 ZnSO4 7
133. 3 phylum division 5 10hm 50um 6 10um 50um 11 II E 4 e
134. D c IET 4 DEEM E configration 5 5 JIS Z 8402 gt H Pp depu 2 z
135. f g h E UE M T i EAT IH 3 Ee k 5 4 5 FEDH inii 5 4 5 1 2i al 8L 5 4 5 2 m
136. No 1 2 2 2 3 4 4 5 2 6 2 7 8 1000 ut 2 9 8 10 1 2 12 1 13 1 14 1 15 2 16 5 17 24well 5 18 500 me 1 19 0 5 me 20 2 21 2 71 No
137. Q14 H T i L E JE 8 Q5 10 um ZEucampia lOu m L S E Nitzschia EY DZIELI WREE LT DRRR E e bR pY gt E A E
138. 5 10 4 T 5 10 4 1 5 10 2 a E b c 5 10 4 2 E 5 6 2 1 1
139. pH D NIU pH DO NTU pH DO NTU A No pHL DO DOC POC OUR 1 1 1 Im 67 EP tT 1m 3 Age 2 60Hz 100V 24 1m 30m 1 277 10f 20 10 50 u m DOC POC 16 25 E 16 5 100mt 5 pH 500mt 5 DO DO 5 10 50 u m
140. E 1 a b 2
141. gt EEK TERR D ES E ghi ems uj 73 HE
142. 10 20 Ciliophora 150um E RE rR Cyclopoida copepodite 80 150um 2 Total EE y ij Total m o 0 44 wr d y y No 2 050917 F T P Im 100ml 100ml L S 050917 11 30 Ciliophora 100um iii z Harpacticoida copepodite 200mm 1 EE ab Total fp Total m E E di mr No 3 050917 F T P Im 120ml 120ml L S 050917 13 10 Harpacticoida 100um Oooo D O o y 4 C Ji Total E 27 rd y y Total m a 82 DRM L 13 35 Total B
143. i 45 1 4 2 5 4 2 1 E
144. 1 3 1 1 3 2 2 1 34 II 3 2 1 C TE G8 500mt 30 3 2 1 EIE 3 0 8S 16 3 3S 3 3 1 S 1 amp 100 2 1 I 3 3 1 3 3 2 S
145. 37 5 ml S S 2 22 45 23 Ditylum brightwellii Skeletonema costatum d urirella sp 1 Thalassiosira spp 051111 1000 ml 37 5 ml S S 3 23 00 E RR Eo LL Skeletonema costatum 85 TIAA LE o u E 37 5 ml 1ml 2 EE uU ICochlodinium sp o y Ditylum brightwellii 4 o Skeletonema costatum 3 1iO Thalassiosira sp 4 EHI os TT EL h M o 37 5 ml ERR ae Ditylum brightwellii Skeletonema costatum 1 aA AE o TT 37 5 ml MAA 23 50 em ES we ox ee Ta Cochlodinium sp 8 Diylumbrighwelli 1 Protoperidinium sp Surirella sp poo Oooo o i S E Prtozoa i o o 3
146. L um iR ERR Noctiluca scintillans 150 2000 Tintinnopsis spp 15 167 Tintinnidium mucicola 50 160 Favella taraikaensis 70 90 Favella ehrenbergii 80 95 Vorticella sp 38 200 Didinium balbianii 60 96 Didinium gargantua 125 130 Keratella cruciformis 40 100 Synchaeta sp 95 145 Brachionus plicatilis 110 176 FEAA Oithona davisae 140 140 Sticholonche zanclea 200 300 Oikopleura sp 300 5000 Oikopleura dioica 500 1300 Podon polyphemoides 500 700 Arcella discoides 90 146 Arcella sp 90 216 Arcella vulgaris 100 152 30 T 4 1 2 S um IRSE Chaetoceros compressum 3 40 Eutintinnus
147. 20 50 u m 200m 10 50m WE 3 50wm 3 10xm 2 1mm 3 20um 2 3 2 DELEGA 3 20 47mm 2 t 47mm 2 47mmGF F 50 2 2 2 or 1 10 202 3 1 2 2 3 1 dere 200 w P ub up E PPS ds up nb Ep b dt PE ind n mam 68 3
148. L 1m 5002 3 9 1 IL 3 9 1 L 3 92 L 1 1m 200 Imm hi 19 5Oum 7 100mt 3 9 1 30um 20um M UEER L 39 0 2
149. L Al IN p E HJ kl J ES 3 dg E Q15 M4 SUR El E A 3 Q16 5 4M A E US dE NEN E F ET mf ind mt Op x
150. mI MR EC Ciliophora 3 om Coelenterata planula J 2 200mm Calanus sp o d 2 300mm PParacalamus aculeatus 20 200300m PParacalamus parvus 8 200mm ICenropages sp 2 300mm Pseudodiaptomus sp _ 42 300mml Japiioceras 3 300uml Temora discaudata 3 300mml Oithinabrevicornis 4 I20mm Oithimasimplex 1 140m Oithona sp 5 100200m Microsetella norvegica 16 lioO lGOml Saphirella like copepods 6 60 I20ml Harpacticoida 6 3 300mml Calanoida copepodite 1 100 200m PParacalamus copepodite 47 503300ml Centropages copepodite 1l 200m Pseudodiaptomus copepodite 3 200mml Labidocera copepodite 1 300ml Oithona copepodite B 100mm Corycaeus copepodite 3 120mm Microsetella copepodite 2 50m Copepoda nauplius J 36 100mm Isopoda 0 1 200um Brachyura zoea 1 300mm EE CE Toa S ITotal m 2786 90 2 1 A P T L M 11 40 Microsetella norvegica aphirella like copepods olychaeta larva elecypoda umbo Cir
151. TSS DOC POC 1 TSS 1 0 4um 60C 1 3 2 WALEZI AAEE TEE 5 BERE 3 2 TH 12 EF pi 60 70 2 0 1mg H 4 41 5 IRET UEY AKR 35g 16 3 5 10 20mt 3 4
152. IL 2 3 QI L S 30zm 10m 35 um 10 m 5um 7xm 5 gm A 17 20 Q2 6 A emm
153. 1 4 3 3 4 3 3 1 4 3 3 2 4 3 3 3 1 n Lor 4 3 3 4 SECOS ni
154. 100mm Polychaeta larva J 1 80 120mm Polychaeta trochophora 7 120mm Pelecypoda umbo J 6 50 6Omml Total m 1920 93 5 RH F LTP 100L 40ml 02 45 03 20 HER nin o E Protoperidinium sp _ 4 Coscinodiscus sp 662 50 eee eee o o o NND EY Tintinnopsis radis 4 O Tintinnidium sp 4 80mm Vericellidae 1 1 200m Synchaeta sp 2 60m Siphonophora 2 j500800um Paracalamus aculeatus 1 200300m Paracalanus parvus 1D 200mm Temora discaudata 1 300m Oithona sp 2 eooo Oncaea sp 2 hopom Corycaeus sp 1 om Microsetella norvegica 1 H00 160um Saphirella like copepods 1 j60 120um Paracalamus copepodite 70 50300m Acartia copepodite 1 o Oithona copepodite iOOmm Corycaeus copepodite 1 1i20mm Microsetella copepodite 1 S0um Copepoda nauplius 1 lim Polychaeta trochophora 30 80 200um Polychaetalarva J 3 lio0250m IPelecypodaumbo 8 leO IOOmm Cirripedia nauplius 2 Soo Cirripedi
155. 1 ZORI lt S I H 1 I dy EE B
156. DOC POC 20 DOC TOC TOC pH 2 POC Q DOC 2 60C
157. 15 00 pa Coscinodiscus granii 50um Coscinodiscus sp 50um Scyphozoa polyp 80 1000um Polychaeta larva 60um Pelecypoda larva 60 200um Podon schmackeri 120um Paracalanus sp 300um Oithona davisae 150um Paracalanus copepodite 250um Oithona copepodite 100 140um Copepoda nauplius 55um Cirripedia nauplius 200um Fish larva 400um Total Total m FTP 050920 80 1 2 2 Im L 10 15 c Coscinodiscus granii 50 60um Total Total f m Coscinodiscus granii 60 90unm Copepoda nauplius 60um Total Total 4l A amp m Im 11 45 Coscinodiscus granii 50pnm Total Total m FTP FTP F T P No
158. 15 MEPC paper G8 E viable organisms 4 Q minimum dimension 4 L 16 E9 E 1
159. 60 90 umol photons m s 5 6 Viability 10 10 viability viability viability 1000 1 m 10 5 viability 300 lm 4 1 5 1 2 16 20 mt
160. TG 04 40 05 50 y 16 6 E E sH 75 En X Chaetoceros affinis Chaetoceros sp Cochlodinium sp CN 14 20 Ciliata Tintinnopsis sp Total 1 122 Dactyliosolen sp Protoperidinium spp Skeletonema costatum Thalassiosira sp J Prorocentrum No 18 BJ 1H BJ ZH SC x PES Chaetoceros affinis i 1000m 80 ml P33 0 mi gt 8 P33 jl ZA Iz RES 4 89 2 2 LV 4 X ZI Z A fs 1 A P T 100L 100ml 30ml S 10 30 A 11 30 pa minimum Pd Cosimodius wp 9 so
161. P 2 1 01 45 OGETEHEE E E EN 218 4 X x X 5 X Ceratium furca Chaetoceros affinis Chaetoceros curvisetus Chaetoceros spp Coscinodiscus spp Ditylum braightwellii 4 ta TE S Q SS S S 3 Ss E 8 amp S SS e d 3 S E HE i ZIN crippsiella sp Thalassiosira sp 1 Hom ooo 1 3336 1000ml _ 275 ml TH Chaetoceros affinis Chaetoceros affinis Chaetoceros curvisetus Chaetoceros sp Cochlodinium sp Coscinodiscus sp X Ceratium fusus d M Prorocentrum 1000 ml S P 2 3 6 Ceratium fusus po 03 Chaetoceros affinis po oS Chaetoceros curvisetus 19 Chaetoceros sp ll Cochlodinium sp 1 Prorocentrum 5 RH E 1000 ml
162. a 34g 500mt 1mo pH 7 20 5 b 38g 1000mt 1 253mt 5 0mt 1000mt 121 1 15 4 5 4 3 1 20 28C 0 5 5 7 II 4 3 1 D 36 40 36 JIS K 0430 72 30 2000 ISO 6222 1988 37 ISO 6222 1988 Water quality Enumeration of viable micro organisms
163. 1 050918 SE 150ml 150ml S 050918 11 30 D No 2 050918 25ml 25ml S 050918 13 10 x FAST BK ENS ENNNNNNNV NEED REEL p E c M o NEN CNN No 3 050918 mu 20ml 20ml S 050918 13 55 DEEP e ud o E D 01 plu 81 No 1 050917 F T P Im 120ml 120ml L S 050917
164. 1 1 RU wr OR RUE 2 Al ACT e HH C39 V CLARA ACTA C D d 57REibC R 4 12 1 3 4 12 1 4 4 12 2 4 12 2 1
165. 2 5 4 7 4 12 4 3 2 4 3 2 1 1 4 3 2 2 a EF b c E d 4 3 2 3
166. 4 9 CHN GFF ORRE CHN 3 DOC 42 IT 1 H ISO IEC 17025 A 1 N ah Tm CO 7 8 9 10 11 1
167. 4 3 2 5 36 1 C 24 2 4 H 4 3 4 T432 6 BdII 43 4 D 36 1C 6 18 100 100 100 Vibrio 4 3 5 Peptone 10 0g Purified water 1000m pH8 6402 121 1C 15 39 B IL 4 3 5
168. 5 10 2 5 10 3 5 10 4 5 10 2 5 10 4 AR 2 5 10 2 a
169. Microbiology Washington D C 20 Compendium of methods for the microbiological examination of foods American Public Health Association Washington D C 21 International Organization for Standardization 22 Standard methods for examination of dairy products American Public Health Association Washington D C 23 Standard methods for examination of water and wastewater American Public Health Association Washington D C 24 2005 2005 25 2004 TE HC 6 596 4 53 Rl 1 50hm 1 10 3 phylum division 5 2 10um 50um 10
170. solution 5 p1 b 30 60 c viability S DIE LT S5 a ee PCIE e ox v M vs oss D xs E a ES se era A at ee e Pe f pesos DR ALD EOM e co poA 2p enu 2 end m s50Hm e mu te av gt AR 102 ERI DEAE AF AQ EDHE Pleurosigma sp 7c L a b Paracalanus parvus EE Coscinodiscus wailesii c
171. spp 18 53 Rhizosolenia setigera 3 87 Helicostomella longa 17 18 Aulacoseira distans 4 20 Stenosemella parvicollis 19 25 Chaetoceros sociale 4 15 Stenosemella sp 19 47 Aulacoseira granulata 5 20 Helicostomella subulata 20 24 Chaetoceros radicans 6 25 T intinnopsis aperta 25 25 Leptocylindrus danicus 6 12 Tintinnopsis beroidea 25 40 Skeletonema costatum 6 22 Tintinnopsis corniger 28 33 Cerataulina pelagica 7 56 Tintinnopsis radix 30 43 Chaetoceros danicum 8 20 Salpingella sp 30 50 Rhizosolenia fragilissima 8 70 Tintinnopsis kofoidi 35 38 Chaetoceros affine 9 30 Amphorella quadrilineata 40 46 Chaetoceros debile 9 40 Tintinnopsis directa 40 47 Chaetoceros didymum 10 40 Tintinnopsis lohmanni 40 62 Chaetoceros lorenzianum 10 80 Leptocylindrus mediterraneus 10 35 Mesodinium rubrum 23 30 Detonula pumila 12 55 Tiarina fusus 30 35 Chaetoceros constrictum 14 35 Actinoptychus senarius 20 105 Trichocerca sp 28 50 Eucampia zodiacus 20 100 Thalassiosira rotula 20 60 Euglypha sp 30 60 TO EE REOR Prorocentrum minimum 15 23 Protoperidinium bipes 15 20 Heterocapsa triquetra 17 29 Gonyaulax verior 26 32 Ceratium furca 30 50 Distephanus speculum 15 35 Ebria tripartita 34 40 ZAN Heterosigma akashiwo 6 15 31 4 3 G8 2 3 5 G8
172. 011 2 viability PERACLEAN Ocean S 5 Tetraselmis sp Calcein AM 100 1 6 1 7 1 8 Tetraselmis sp e Calcein AM L 5 nr Ek SN e d y 200 um Calcein AM E Calcein AM I p EBIT 2Xefamm Limo OEGEOIE G MWE ko TR l RRIA
173. 1 984 Vibrio cholerae gouup cfu 100ml TCBS agar 237C 6 H fi i3 3 0 3 7 2 3 10 0 ND 9 3 ND 1 120 509 ND 2520 ND ND group cfu 100ml mEnterococcus agar 23 C 6 cfu 100ml XM G agar 23 C 6 cfu 100ml XM G agar 23 C 6 H B fe e ND ND NDJ png 1 4 F P T 8 0 ND 4 0 ND ND Nb 120 0 ND ND 120 0 2005 9 16 2005 9 17 2005 9 18 DO E Turbidity a DOC POC 84 2 2 1 S 1 No 1 H 051111 Hu 1000ml MADE 37 5 ml 22 30 RER infa Chaetoceros curvisetus 1111 rp Ditylum brightwellii 2 j 051111 1000 ml
174. 2 13 14 O 014202 C2 P5 N wes Heo does ES R dms R e Saa m B uS 10 OU UT CO OU PU OU SL SOUCI SV mn O oo Ob 4 tQ nM 1 1 1 IH 43 1 2
175. 4 0 2 4 3 h a F Vibrio cholerae O1 and 0139 Pancreatic digest of gelatin Brain heart solids from infusion Peptic digest of animal tissue NaCl Na HPO4 Purified water 1214 1 C 15 y SiS FEZ AGAR LC HV s Brain Heart Infusion Broth 14 5g 6 0g 6 0g 5 0g 2 5g 1000mt D 2 X toxiogenic Vibrio cholerae 1lg 1 4 3 2 1 1 TCBS Thiosulfate Citrate Bile Salts Sucrose Agar TCBS
176. 4 3 3 20 200 100mt N D Not detectable 352C 18 24 X IL 4 3 3 UV 36enm ImC 36 4 3 3 Enterococcus group Intestinal Enterococci 1 03 4 3 2 1 Q M
177. 4303T Washington DC 35 MI medium No 3 Proteose peptone No 3 50g Yeast eExtract 3 0g B D B D lactose 1 0g 4 8 D 6 4 Methylumbelliferyl 8 D Galactopyranoside MUGal 0 1g D Indoxyl B D Glucuronide IBDG 0 32 g NaCl 7 5g K HPO 33g KH PO 1 0g Sodium lauryl sulfate 0 2 g Sodium desoxycholate 0 1g Agar 15 0g Purified water 1000m pH 6 95 0 2 121 1C 15 3 4 5 6 AIR 43 1 G 02um E 47mm 4 3 1 4 Ed II
178. 8 POPA SUCI No IL Em 77H 5 1 ml isterionellopsis glacilis Bacteriastrum sp Chaetoceros curvisetus i Tintinnopgsis sp 1 D 1 j i oU i 75 S0509 unknown zt S050916 3 LEE O EE legg growth 1j MU m 277 Umbilicosphera sp 50916 No 50916 No F4 10 00 10 36 _ _ j 9 SO509176 10 50 _ WEE T 77Nx 2 1L
179. Biosafety Manual E ISO IEC17023 4 4 i ES 2 N E E E BS2 HOX VE
180. Colony count by inculation in or on a nutrient cluture medium 38 ISO 9308 1 1990 Water quality Detection and enumeration of Escherichia coli and coliform bacteria Part 1 Membrane filtration method Phosphate buffer solution 39 Standard Methods for the Examination of Water and Wastewater 21th edition 2005 American Public Health Association Washington D C Buffered water 40 Standard Methods for the Examination of Dairy Products 17th edition 2004 American Public Health Association Washington D C 34 5 20 200 Ed IL 4 3 2 Imt X M 4 3 2 4 3 2 Coliform Escherichia coli 1
181. Ev E 4 13 3 E E 4 13 4 4 14 4 14 1
182. H O 8x10 CoCl 6H2O 5x10 MnCl 4H O 9x107 Thiamin HCI 3x107 Biotin 2x10 Cyanocobalamin 1x10 mol 6 24 well 1 well 1 ml O 4 1 8 a 5 Omura et al 2003 La mar 41 86 93 Guillard In Smith W L and Chanle y M H eds 1975 Culture of Marine Invertebrate Animals Plenum Press New York USA 26 viability 4 1 8 b c 1 well 1 SUIL4 L 8 12
183. ability Bd IT 4 1 7 IH Alis 3 le 2 B e 3 1 e SIS 4 mo 3 Le d Rp j 417 HRM 2 viability 3 viability 1 viability D 2 80 20
184. acypris 1 oo Brachyurazoea 1 300mm Toa a ITotal m 204600 94 Su 4 po dd iid Pr EX CERGEUERUECEOKTESRUmD Marne ar23 C H KERAMET cirm R2A egar2Y COH BRE Vibrio cholerae gouup cfu 100ml TCBS agar 23 C 6 228 1180 0 860 0 _ ND 3400 44 10 ND NO NDL Noe e 300 0 cfu 100ml XM G agar 23 6 0 ooo 6 355 5 800 0 ABEC 100m XW G asc CSB RISE 16 12 4 8 0 ND 2 4 lt x V2 2005 11 11 2006 1 20 2005 11 11 DOC POC 95 5
185. arva 60 200um 60 I Podon schmackeri 120um pro oil Paracalanus sp 300um 30 p Oithona davisae 150um 285 Corycaeus sp 130um BOO Paracalanus copepodite 250um 1 J Acartia copepodite 250um E ae O e l Oithona copepodite 100 140um 1 J Harpacticoida copepodite 200um 153 Cirripedia nauplius 200m I 3L Sp 1j Cirripedia cypris 200um DENEN NEXT EE EE I Total Oa y Total rm Oo o oma qd 78 20L 13 00 Item Protoperisinium oblongum 50um Protoperisinium sp 0L77 Coscinodiscus granii 50um Coscinodiscus sp 50um Tintinnopsis sp 80um Scyphozoa polyp 80 1000um Polychaeta larva 60um Pelecypoda larva 60 200um Podon schmackeri 120um Paracalanus sp 300um Oithona davisae 150um Corycaeus sp 130m Paracalanus copepodite 250um Oithona copepodite 100 140um Copepoda nauplius 55um Cirripedia nauplius 200um Cirripedia cypris 200um Brachyura zoea 300um Total Total m FTP 050920 79 20L L
186. d determinations of marine plankton Stain Technology 49 97 104 103 a 2006 2006 50 54 Koike et al 2010 Thermal Aqua Filtration TAF System A New Concept of Environment Friendly BWMS Applying Retrieved Heat to Eliminate Living Organisms FF xd WMU Journal of Maritime Affairs in press 2 8 kazkoike g hiroshima u ac jp 104
187. e O1 O139 Escherichia coli Interstinal Enterococci G8 2 3 20 1 o 32 4 3 1 Heterotrophic bacteria D
188. es for Approval of Ballast Water Management Systems G8 2008 10 MEPC 58 37102 0094 HN BH EN CH MEPC 174 58y 3 32 TEL 03 3261 6611
189. etence of testing and calibration laboratories IDT 1 JIS Z 9358 4 1 3 B 2 JIS Z 9358 4 4 1 4 1 1 44 4 1 2
190. nsion 1 1 H 4 1 4 4 1 5 II 4 1 6 era 1 C CR A 3 E II 4 1 4 1 1 GEHEE es MORES 1 09000000000 4 1 5 1 ERM 4 IL 4 1 6 2 ER a 1 b c 24 4 1 4 viability D viability viability Oe utc n Calcein AM 6 1 vi
191. other guidance documents and other general quality control information are available from appropriate international organizations ISO17025 QMP QAPP S017025 T 2 The QMP addresses the quality control management structure and policies of the testing body including subcontractors and outside laboratories QMP 3 The QAPP is a project specific technical document reflecting the specifics of the BWMS to be tested the test facility and other conditions affecting the actual design 12 and implementation of the required experiments QAPP iX BWMS
192. ripedia nauplius CA RS oo N t A cj99 e s u m r2 o o N Total 4l amp m ERES 518 051111 Jed 100 100ml 10ml 01 10 91 3 i HW v4 ADP T F 100L OOOO O DRED n 100m 20ml Lnr Jy as P 1 01 10 02 05 MM zm R CA e Ta z 200 300um 120um 200um 250um 140um cartia sp Cn c c T T aphirella like copepods CN P N T cartia copepodite 200um 100um e Total m 92 4 AP OSUU LESE uS a NAA T EXE mL p ETTE i pd EA puc el CTI m c t M M ti X I S Hi Synchaeta sp sm Calanus sp 9 1602220m Oithona sp 6 23 eoo Saphirella like copepods 3 60 120um Calanus copepodite J 1 oO Oithona copepodite 2 100mm Copepoda nauplius J 32
193. tandard methods for examination of water and wastewater 21th ed American Public Health Association Washington D C 31 American Public Health Association 2001 Compendium of methods for the microbiological examination of foods 4th ed American Public Health Association Washington D C Colony forming unit Spiral Plate Method Reasoner D J and E E Geldreich 1955 A new medium for the enumeration and subculture of bacteria from potable water Applied and Environmental Microbiology 49 1 7 34 Zobell C E 1941 Studies on marine bacteria I The cultural requirements of heterotrophic aerobes Journal of Marine Research 4 42 75 35 German Collection of Microorganisms 33 3 ARW pH7 2 7 5 CHIRE L 7 0 ALERTER GET Ze TEE D D BUE OD SACK IRAE L T Phosphate buffer solution KH PO 42 5mg MgCl 6H O 190mg Purified water 1000 mf
194. ty E scope 1 I PS 57 E lt AN RD gt
Download Pdf Manuals
Related Search