PCRキット 取扱説明書ver1.3
Contents
1. Feel so Bio TEL 03 6277 8041 FAX 03 6277 8042 Mail info feelsobio net FAX FAX http wwwleaveanest com 160 0004 135 0016 2 2 20 nes 2 8 5F TEL 03 5632 9610 FAX 03 5632 9619 TEL 03 6277 8041 e mail mail cosmobio co jp FAX 03 6277 8042 URL http www cosmobio co jp2. Mupid S5 100mL SN _DNA DNA 5 L PCR 3 3 x PCR 35 gu L DNA 2 L _ DNA PCR FE DNA
3. DNA TAE TAE DNA DNA DNA Loading buffer DNA DNA DNA ts Ak 0 TAE buffer DNA DNA DNA
4. 72 C DNA DNA m m la i me DNA mm 1 2 3 4 10 30 I 3 DNA PCR DNA dNTP DNA 9 DNA RNA
5. DNA DNA DNA DNA base pair bp DNA DNA bp base pair DNA DNA
6. 50C 200mL 8 4 4 1 0 7g 100ml 11 PCR gt 1 DNA
7. DNA DNA DNA 2 NO NM RDNA DNA DNA EtBr Mupid Blue EtBr DNA DNA
8. Mupid Blue 10 40 975mL 40 Mupid S 100mL 1 300mL 1 4g 1xTAE 200mL 2
9. DNA DNA DNA TE 20 C PCR DNA 500bp DNA TE 207C DNA dNTPs MgCl PCR 2000 PCR 1 DNA RNA 20C PCR 200ul PCR
10. Mupid S 100 20 DNA TAE DNA 13 DNA Mupid Blue DNA gt Mupid Blue 50 DNA DNA 50 DNA DNA DNA
11. DNA DNA DNA DNA DNA DNA DNA DNA
12. TBE buffer DNA DNA DNA DNA DNA DNA 0 79 _ DNA bp _ 06 1 000 20 000 O 7 800 10 000 1 Ho00 7 000 1 400 6 000 15 200 3 000 2 100 2 000 16
13. PCR DNA 40 TAE EDTA DNA 4 C 40 4 1 2 DNA 12 L PCR F 12 L PCR R 12 L 5 L
14. DNA 1 COCOOOO DNA se UEzs DNA DNA 94 2 DNA 1 55C 60C DNA 20 1 DNA DNA 1 DNA DNA 2 DNA GC
15. DNA 70 L 1 PCR F 70L 1 PCR R 70 L 1 200 L 1 200 L 1 100L 1 DNA 60 L 1 40 25mL 1 29 1 PCR 20 1 50 1 1 1 20L 5 1 200 gL 1 6 1 1 2 2 DNA Mupid Blue Mupid Blue p15 5 1 5 1 Mupid 5
16. 1 RNA 1 RNA 1 DNA DNA DNA Thermus aquaticus DNA Taq Taq DNA PCR PCR DNA DNA DNA 8 8 0 BO 3 5 ejejejejele ors Il I 3 no lalla lalla la lalla allelel ole a alel gs Taq DNA Lay 68 5 sl OH3 gt 15 2 DNA TAE buffer TBE buffer TAE buffer kb DNA
17. 35L 18uL DNA lOuL 40 100mL 40 1 PCR PCR Polymerase Chain Reaction PCR DNA PCR PCR PCR 20 DNA DNA 3
18. PCR F PCR R 2 DNA PCR F PCR R 5uL PCR 3 2 PCR 15uL 4 3 PCR 95 C 3 95C 30 58C 30 35 72C 45 72 C 5 4C Hold DNA 12
19. 1 100 003 Feel so Bio 19 003 PCR Ver 1 3 2 3 4 5 PCR 6 8 11 12 13 DNA 14 1 DNA 15 2 16 polymerase chain reaction PCR DNA PCR PCR PCR 1980 PCR 20
20. 50 DNA 2 2 DNA 14 1 DNA DNA RNA 3 DNA 2 DNA 2 DNA RNA 2 DNA
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