アレルギーに関する調査研究Ⅱ
Contents
1.2. 1404 25 7 21 25 8 29 40 25 9 12 54 25 9 26 68 400
3. 1 125 g 145 g 160 g 210 g 1 170 g 220 g 28 L 400 2 3 78 5 FASPEK
4. 2 3 7 2 3 RSD 10 9 1
5. RN B 1 17 1 0g 19 mL 15 100 3 cm pH pH6 0 8 0 pH 23C 3000 g 20 min 100 pL 25C 1 300 pL 6 100 uL
6. 1 2 ELISA PCR Polymerase Chain Reaction 2
7. 2004 2010 2012 2010 2010 AtoZ 2009 2011 2012 2012 2011 2012 2012 NHK
8. 1 13 23 13 3 15 79 2 K Fujino H Funatsuki M Inada Y Shimono and Y Kikuta 2001 Expression Cloning and Immunological Analysis of Buckwheat Fagopyrum esculentum Moench Seed Storage Proteins Journal of Agricultural and Food Chemistry 49 4 DD 1825 1829 3 K Tanaka K Matsumoto A Akasaka T Nakajima T Nagasu Y likura and H Saito 2002 Pepsin Resistant 16 kD Buckwheat Protein is Associated with Immediate Hypersensitivity Reaction in Patients with Buckwheat Allergy International Archives of Allersgy and Immunology 129 1 pp 49 56 4 S Y Choi J H Sohn Y W Lee E K Lee C S Hong and J W Park 2007 Characterization of Buckwheat 19 KD Allergen and Its Application for Diagnosing Clinical Reactivity International Archives of Allergsy and Immunology 144 4 pp 267 274 5 14 11 6 1106001 6
9. 2013 10 2011 2011 2011 2011 2008 2003 2009 2008 2013 2012 2013 2013 Vol 31 No 17
10. g g 1 Mean SE 3 relative standard deviation RSD 7 8 RSD 10 n 3 1 RDO 0 16 0 007 7 8 0 24 0 007 5 0 0 021 0 001 12 6 289X10W 810 2 4 8 4 x10 613 3
11. Q amp A http www caa go jp foods qa allergy01_aqa html 37 38 2013 Derma 2013 6 No 205 2011 2013 2011 2011 2012 2012 2012 2012 2013 2013 2012 2003
12. 25C 30 300 uL 6 100 uL 25C 10 100 uL 0 415 nm 1 g 10 ug
13. 3 3 RSD 6 1 RSD 2 n 3 mg g mg g ug mL Mean SE Mean SE Mean SE 54 4 1 2 3 8 32 8 1 4 7 32 2 FE 1 5 9 2 76 0 0 7 1 6 50 5 0 2 0 7 18 4 0 2 1 5 1 91 7 0 6 1 2 672 15 3 8 23 6 0 4 27 45 88 8 0 9 17 607 28 7 9 10 3 E06 10 9 40 35 36 3 n 3 ug g ug g Mean SE Mean SE 0 24 0 02 18 5 0 63 0 002 0 68 9 2 OOMS 2 2 11 8 0 08 1
14. 16 kDa Fag e 3 19 kDa Fag e 2 20 40 65 90 J S G
15. ELISA Enzyme Iinked immunosorbent assay 1 FASPEK 1 Z CR 3 4 EE x x 33 34
16. OO 2 8 45 0 15 0 004 6 1 N D NIDN 50 as 0 047 0 008 30 2 40 N D Not Determined 7 9 12 0 ug g 1 1
17. PCR ELISA ELISA 1 1 1 7 0 13 mg g
18. of allergy 1 me Fag e 1 Fag e 2 Fag e 3 3 Fag e 1 13S jp 22 kDa Fag e 2 2S
19. 7 1 4 1 2 10 3 32 2 ug mL 1 28 L 0 90 g 0 52 g 0 66 g 0 29 g 0 72 0 36 0 41 0 14
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