テキスト[PDF:11.6MB] - 農業・食品産業技術総合研究機構
Contents
1. 20q FE Ew 1 20 Ne 2 72 72 2 1 05 50ml 1 500 1 40 50ml2. _ 2 Pratylenchus 1 8 0
3. MC 2004 8 247pp No 8 2004 5 99pp I 1 Meloidogyne 1 3 3
4. 4 11 000 12 000 100 5 Ditylenchus I 1 p 78 86 1 2 9 10a 9
5. Pratylenchus penetrans co ae P vulnus PCR 2 DNA TW81 AB28 RNA ITS DNA 7777f 1 1 1 DNA PCR zpf I 2 C23 1 1 PCR DNA 2 DNA 3 4 PCR DNA 5 PCR
6. 17 10XGreen Buffer 2 PCR 10 FastDigest HinfI1 ul 37C5 60C20 5 1 0XTAE 1 5 PCR PCR 5ul 6 100V 50 UV gt PCR imfl TW81 5 GTTTCCGTAGGTGAACCTGC 37 AB28 5 ATATGCTTAAGTTCAGCGGGT 37 Joyce et al 1994 Cost 812 pp
7. 90 50 4 gall index 4 X 100 CO 2 p 105 106 0 4 0 100 3 p 106 108 0 4 0 100 IV 1
8. 6 1 5 7 f1 1 5m1 87C5 8 PCR 100V50 1 DNA DNA 20 8 PCR 1 55 30 95 10 15 80 ul DNA 5 100 ul ISOHAIR 0 01XExtraction Buffer 18 5 ul SDS TE Enzyme Solution 1 ul Proteinase KK
9. 3 Heterodera Globodera 1 2 4 Aphelenchoides NE TS EEA TREN AE
10. 8 0 3 0 4ha 8 3 20cm 4 5 6 1 7 8 3 9 50cem X50cm 1 10 Y 16 1ha 2 p 86 95 1
11. p 9 24 2 A H G F B C I B C D E J D E I 3 p 11 16 7 Perennial pattern 9 1 2 KU 10 1 Meloidogyne incognita Pc 0 a we Ni os a 5R 3 _ A mi th eh KS
12. 3 1mm 5 150um 2 4 150um 30 38um 2 5 88um 38um 4 6 30 25um 3 p 95 100 1 p 103 105 0 1
13. 1 3 mw 6 Rm ae eo 2 6 p 50 55 Aphelenchoides fragariae 4 C23 2 5 10 3 3 Pratylenchus penetrans P coffeae P vulnus PCR RFLP PCR RELP Polymerase Chann Reactnon Restriction Fragment Length Polymorphnsm PCR DNA DNA PCR DNA
14. 10g 20ml 40 10ml 1 500 1 20 40um CO DD 3 25lm DA mm WW 2 1 2mm
15. NN a qa a wu es 3 2 8626 20 BRSSSSSSSN 9 4 p 17 20 1 Pratylenchus penetrans WW 1 a a J Y f a ee 4 _ a NT _ 9 AA gt be a J g TI NY ER Sc a 4 ii __ cae v m De 4 aC No 1 A 2 Zcrenatus 3 eg7ecZzzg 4 vulunus 19 5 p 23 28 1 eZezogezg glycines 18 3 Heterodera tritolii 2 SR 2
16. 2 5 011 857 9490 2 5 gt a RN I A SE IB 2 21 10 2 10 4 3 1 1 3
17. Lysis Solution 0 5 DTT 20 1 2 PCR PCR 18 1 DNA 2 20 ul 94 2 35 x 94 30 55 80 72 1 15 2 PCR Quick Taq HS DyeMix 10 10 uM 0 4 7 2 18 3 1 5 50 ml 1 0XTAE 50 ml 0 75 g 10 000 5 30 60 C23 4 FastDigest imfT
18. 178 187 Zheng et al 2000 Russ J Nematol 8 109 113 PCR REFLP Orui Y 1996 Appl Entomol Zoo1 31 4 505 514 Orui Y and Mizukubo T 1999 Appl Entomol Zool 34 2 205 211
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