T4-RD1/T8-FITC
Contents
1. No Wash No Wash Q Prep TQ Prep Q Prep TQ Prep 1 2 3 4 T4 CD4 DiGeorge2. 1 2 3 EDTA 24 4 9096 5 10 000 mm 10 000 mm 6
3. 7 8 9 ow BRIZ X EE E37 FD Jb 5i f X E CYTO STAT COULTER CLONE MSlgG1 RD1 MSIgG1 FITC 10 1 10wL 1 A TQ Prep Q Prep
4. LH700 3 10 x 103 mm 3 4 5 1 Q Prep 1 12mm x 75mm 2 100 L 3 10 L MSlgG1 RD1 MSlgG1 FITC 6603796 10L 4 10 5 TQ Prep Q Prep
5. T4 RD1 T8 FITC 6603802 2 8 C 6 2 8 C 20 25 C T4 RD1 T8 FITC 6603802 50 0 5mL 1 2 20 21 22 McMichael AJ ed Leukocyte Typing ll White Cell Differentiation Antigens 1987 Oxford Oxford University Press p 202 206 Morimoto C Letvin NL Distaso JA Aldrich WR and Schlossman SF 1985 The isolation and characterization of the human suppressor inducer T cell subset J Immunol 134 1508 1515 Morimoto C Letvin NL Distaso JA Brown HM and Schlossman SF 1986 The cellular basis for the induction of antigen
6. CYTO TROL 6604248 Fc 2 Quadrant1 2 4 29 1 19 65 n 25 T4 T8 Min Max Mean 1SD T4 35 62 46 6 T8 9 17 43 27
7. 3 10 x10 mm 20 25 C 6 a gt 10 x 10 mm 10 20 x10 2 20 30 x10 3 30 40 x10 5 40 60 x 10 6 60 100 x 10 10 100 200 x10 20 b 3 x 10 mm 1 25C 500 x g 5 2
8. 6603802 2009 11 2007 2 20100EZY00146000 T T4 RD1 T8 FITC 1 2 3 4 1 T 14 0 055 u gr AF 2 T T8 0 275 g T4 RD1 CD4 62kD CD4 8096 kno T 609
9. T4 RD1 T8 FITC 1 T4 RD1 T8 FITC 10 6 1 T4 RD1 T8 FITC 29 2 2 3 CV T4 RD1 T8 FITC 5 596 n 51 T4 RD1 T8 FITC CD4 CD8 T4 T8
10. CD4 CD8 SLE CD4 T4 T8 SLE SLE CD4 CD8 T4 T8 SLE T4 T8 T4 T8 CD4 CD8 T4 T8 CD4 cell line
11. SCID AIDS AIDS HIV CD4 CD4 T8 CD8 DiGeorge SCID B Epstein Barr CD8 T4 T8 CD4 CD8 CD4 CD8 T4 T8 T T4 T8 T4 T8 CD4 ARC AIDS related comple
12. 0 5mL 6 2 2 2 8 C 24 2 1 12mm x 75mm 2 100uL 3 10 L MSIgG1 RD1 MSIgG1 FITC 6603796 10 L 4 45 2 4 5 PBS 2 3mL 400 450xg 5 6 7 PBS 25 1mL In
13. CD4 CD4 T T T B T CD4 class MHC T8 FITC CD8 76kD 32 34kD CD8 80 LRO T 30 lt 359 CD8 CD8 class MHC T4 SFCI12T4D11 T4 t rR T BALB cJ NS 1 AG4 T8 SFCI21Thy2D3 T8 BAL8 cJ NS 1 AG4
14. NK null 3 T T CD4 T CD8 2 T T CD4 CD8 T4 T8 T T
15. 6 T4 mm 575 1734 989 287 T8 mm 327 1148 573 193 CD4 T T4 RD1 CD4 T TA lt T T4 CD8 T NK T8 FITC NK CD8 T NK T8 T T8 CD4 CD8 T CD4 CD8 T T B
16. 7546999 300 TQ Prep 7546946 100 Q Prep B 6603152 300 2 PBS PBS 6603369 1 500mL pH 7 2 0 2 3 MslgG1 RD1 MslgG1 FITC 6603796 50 0 5mL EDTA 1 100 ux L 1 1 lt 2mL
17. lg lgG1 H z L H T4 T8 T4 T8 T4 T8 SR BB T4 RD1 RD1 Phycoerythrin PE RD1 0 5 1 5 486 575nm 568 590nm T8 FITC FITC Fluorescein isothyoyanate FITC 5 10 468 509nm 504 541nm 1 0 5mL BSA 0 296 0 01M NaCl 0 15M NaN3 0 196 T T 2 488nm 2 FITC RD1
18. y 0 94x 1 7 r 0 959 y 0 97x 0 2 r 0 961 y 0 98 x 0 1 r 0 948 1 0 19 2 3 4 5 6
19. 30 2 8 250 L 9 PBS 2mL 10 400 450xg 5 11 12 9 gt 11 13 PBS 500 uL 14 1 2 FS 90 SS 3 FITC Log RD1 PE Log
20. 2 RD1 PE FITC Quadrant 1 A1 T4 T8 Quadrant 2 A2 T4 amp T8 Quadrant3 A3 T4 T8 Quadrant4 A4 T4 T8 1023 FS BNET T8 FITC SS T4 T8 COULTER LH700 mm mm x 9 x 10 Q Prep Flow Count 7547053 IMMUNO TROL 6607077
21. EL Haynes BF Nadler LM and Bernstein ID eds Leukocyte Typing Il Human T Lymphocyles 1986 New York Springer Verlag p 8 9 26 Koepke JA and Landay AL Precision and accuracy of absolute lymphocyte counts Clin Immunol Immunopathol 1989 52 19 27 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 7135 0063 5 7 TOC oe COULTER 6603802 0911
22. nalysis Am J Med Sci 289 139 147 Morimoto C Reinherz EL Schlossman SF Shur PH Mills JA and Steinberg SD 1980 Alterations in immunoregulatory T cell subsets in active systemic lupus erythematosus J Clin Invest 66 1171 1174 Raziuddin S Nur MA and Alwabel AA 1989 Selective loss of the CD4 inducers of suppressor T cell subsets 2H4 in active systemic lupus erythematosus J Rheumatol 16 1315 1319 Sato K Miyasaka N Yamaoka K Okuda M Yata J and Nishioka K I987 Quantitative detect of CD4 2H4 cells in systemic lupus erythematosus and Sjogren s syndrome Arthritis Rheum 30 1407 1411 Ramos EL Turka LA Leggat JE Wood IG Milford EL and Carpenter CB 1989 Decrease in phenotypically defined T helper inducer cells Cr4 4B4 and increase in T suppressor effector calls 8 2H4 in stable renal allograft recipients Transplantation 47 465 471 23 Pedrazzini AS Freedman AS Andersen J Heflin L Anderson K Takvorian R Canellos GP Whitman J Coral F Ritz J and Nadler LM 4 4 1989 Anti B cell monoclonal antibody purged autologous bone marrow transplantation for B cell non Hodgkin s lymphoma Phenotypic reconstitution and B cell function Blood 74 2203 2211 24 Reinherz EL Kung PC Goldstein G and Schlossman SF 1979 A monoclonal antibody with selective reactivity with functionally mature human thymocytes and all peripheral human T cells J Immunol 123 1312 1317 25 Reinherz
23. oberts PL and Handsfield HH 1987 Cytomegalovirus infection in homosexual men Relationship to sexual practices antibody to human immunodeficiency virus and cell mediated immunity Am J Med 82 593 601 Taylor MGJ Fahey JL Detels R and Giorgi JV 1989 CD4 percentage CD4 number and CD4 CD8 ratio in HIV infection How to choose and how to use J AIDS 2 114 124 Morimoto C Hafler DA Weiner HL Letvin NL Hagan M Daley J and Schlossman SF 1987 Selective loss of the suppressor inducer T cell subset in progressive multiple sclerosis Analysis with anti 2H4 monoclonal antibody N Engl J Med 316 67 72 Reinherz EL Weiner HL Hauser SL Cohen JA Distaso JA and Schlossman SF 1980 Loss of suppressor T cells in active multiple sclerosis Analysis with monoclonal antibodies N Engl J Med 303 125 129 Weiner JL Hafler DA Fallis RJ Johnson D Ault KA and Hauser SL 1984 Altered blood T cell subsets In patients with multiple sclerosis J Neuroimmunol 6 115 121 emofen JS Chused TM Leiserson WM Reeves JP Alling DW and Steinberg AD 1982 Heterogeneity of immunoregulatory T cell subsets in systemic lupus erythematosus Am J Med 727 783 790 Smolen JS Morimoto C Steinberg AD Wolf A Schlossman SF Steinberg RT Penne E Reinherz EL Rerchlin M and Chused TM 1985 Systemic lupus erythematosus delineation of subpopulations by clinical serologic and T cell marker a
24. specific T8 suppressor cells Eur J Immunol 16 198 204 Reinherz EL Meuer SC and Schlossman SF 1983 The delineation of antigen receptors on human T lymphocytes Immunol Today 4 5 8 Reinherz EL Morimoto C Fitzgerald K Hussey RE Daley JF and Schlossman SF 1982 Heterogeneity of human T4 inducer T cells defined by a monoclonal antibody that delineates two functional subpopulations J Immunol 128 463 468 Meuer SC Schlossman SF and Reinherz EL 1982 Clonal analysis of human cytotoxic T lymphocytes 14 and T8 effector T cells recognize products of different major histocompatibility regions Proc Natl Acad Sci USA 79 4395 4399 Reinherz E Hussey HE Fitzgerald K Snow P Terhorst C and Schlossman SF 1981 Antibody directed at a surface structure inhibits cyiolytic but not suppressor function of human T lymphocytes Nature 294 168 170 Reinherz EL Cooper MD and Schlossman SF 1981 Abnormalities of T cell maturation and regulation in human beings with immunodeficiency disorders J Clin Invest 68 699 705 Schmidt RE 1989 Monoclonal antibodies for diagnosis of immunodeficiencies Blut 59 200 206 de Martini RM and Parker JW 1989 Immunologic alterations in Human immunodeficiency virus infection A review J Clin Lab Anal 3 56 70 Fauci AS 1988 The human immunodeficiency virus infecfivity and mechanism of pathogenesis Science 239 617 622 Collier AC Meyers JD Corey L Murphy VL R
25. x AIDS AIDS CD4 T4 T8 0 CD8 3 4 T4 T8 CD4 CD8 MS SLE MS T4 T8 CD4 CD8 SLE CD4 2 TA T8 SLE CD8 TA T8 SLE
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