基本性能 - 化学・生命系
Contents
1. laenlly ee 209 0726 TIC TF 1 Epigallecatachin 289 0731 2 Catechin 1 3 Eplcalechin 1 Ndh PaP AVI 5 1 1 gt La g 6 ei 3 E catachin 2 1 EGS Egallocatechin llate EE 5 EnlCnlachln gallnte 09 4 Epigallocatechin gallate lh i 1 felrn2. INITE H OY NK 160 OOO4 28 8 03 5379 2340 WR http www hitachi hitec com fielding O1820 203 813 9iO00 17 30 E Mail bunseki cs hisco co jp HTB 182P 2009 8 Printed in Japan H e LIT MS e NF aaj ll LIT q TOF _ NanoFrontier eLD LIT TOF
3. L 2200 QDAD nanoLC 50nL min 50n L min DEGS Dual Exchange Gradient System 50nL min 2100 x2 1 2100 L290 DEGS
4. MS ER EA i Mass Deviation ppm LIT TOF MS q TOF MS MS i SA in MS 3 MSrMI57iMIG NT MS 0 0016D MS MS 00UUO6DMS3 00005DaE L expectsd maBs 7 4BBB52 We Sample Glu1 Fibrinopeplidm EB 1569 690 OOO16Da TIM mm Ya 47 11 arHmWNOTm LITE 1 HE GDB A Bry J 0006pa 1 Fi 05WLD NS 734 RMS error 0 0014 Da fragment measured mass Dal 175 1207 246 1576 333 1897 80 2568 527 3259 684 3469 B13 3 1 4
5. TDC Time to Digital Converter 1 1 10fg 200pg Smple Resernine R 0 998 pg CasH4oNz05 608 27 i 3s Fi mn FF a Sample Melatonin C13aH1eN202 232 12 MS MS Target Exclusion Target Inclusion
6. TOF LIT TOF RE ADC Analog to Digital Converter 1 MS MS Da a TI esolution _ 997 2137 397 2127 16100 448 1976 448 1971 lt 1 1 16200 1 T1418 Ample Ingullm Bovine Fantress 1 LIT TOF LIT CID LIT LIT LIT
7. Ervthromycin 109 nH Ta LN W RE ED Te Ep0 9 Fg 3 LL i i a NUMmm ami sm Na il W Ni 280 M0 GH 9 Em_A ET Ta MEN me TEE 15B 1170 HO Rie Gb Intmnaity We TE RN PT Ey ft ri mr MS MIS MS i mE ps Error maj Error ppm Botope Match Score 0 0 999951 0 997661 0 999067 Camlp mmma 1 TH Gr Ha NM Ors TEE CmhinhN Oi GH Os MS 718 47388 MSMIS 560 38107 Ms 15H 11704 MS atbto EE MS b a c Em_A iT ET 158 11881 158 U9G3 1H 255BB 560 38107 158 09281 02 25403 1 fB 19811704 Em A 1 MS MS 560 a
8. MS MS MS EE EE iT a MS MS MS Event MS MS Event e ee e MS MS re 1 eiTRAQ Sequence Program asia SM 8 Br mmiti lt Pi Tr We Fe mm meet i Ra Eo a 3 a http www matrixscience corm me Eh i MS MS Et SM Eee MS I 9 9 i Fm PEAKSN ee MS MS MS MS 1
9. Isotope Match Score XITandem The Global Proteome Machine Organization me e WII PU semitmicro ESI AEBGT ESI AECI D 2 MS fF MS
10. MS MS MS LIT 3 10 q TOF P7 LIT TOF P7 Sample Spiramycim LIT 7 1 10 h 1 lg 3DG TOF LIT TOF 1 MS SS Faml 3 190 0 m i 0 i 30 30 19 g bk NanoFrontier eln _ i 5 3 Counter Gas TOF
11. NanoFrontier eln 2 IBA A B IBA IBA A DB WT a pb 1 DB B B MS MS a MS MS A WP DB B A MS MS AA DB Desorption Peak MS
12. MS MS TBA NanoPFrontier eLD MS MS 1 MS MS MS MS 7 IBA ml TE 199 15 Flite TE Fieitlewl Test MS iM kab LG5 MS HE 1 laBed DB i i wm 577min 577min 1 9 MS M8S RT 2 5Z7min b d a b cd MS MS Target Exclusiaf Tabiet
13. LIT 4 MS MS QuECD MS IBAT Information Based Acquisition ADC Analog to Digital Convertcr LC MS e q MS MS eECD MS MS TO qq quadrupole Na no rontier BA NEDO LIT q TOF MS High sensitivity High resolution Nano ESI High accuracy High speed scan LC nano LC semi micro LC nano ESI semi micro ESI APCI micro ESI High sensitivity LIT 9q LIT LIT q
14. i 1 1 2300 1 2100 UIV y Dual Exchange Gradient System a rr i Ee rhs Gi BLM EE mm BK150OmmLi HzO in 0 1 5 Formic acid IManH In 0 1 Formlc ad B0 g0 0mir 50 5Q 1Bmin euL nimi A 308 0681 ESI 1 Enigallgnatmchln 3UOUV re 10D 1000 7 2 ft TI A 7 a i i Fj T i ar Ny 4 FR hh i 1 0 A 7 WM Ah Im i 1
15. LC MS MS 3 LIT TOF q TOF TOF QuECD QuECD MS MS CID Collision Induced Dissociation MS MS CID ADC Analog to Digital Converter NanoFrontier 1 LC nano LC semi micro LC LC MS NanoFrontier eLD 4 LIT TOF TOF a LIT q TOF MS SY lt NEW ho LIT TOF MS
16. b c MS 158 b 2 Em_A 16 Em_A c 1 ErythromycinB EET MgE560 TOF rh ri ITHAGW 114 11 116 117 1 BI2ET1D BSAILVNELETEFAK ets 1H11 FIT LT Tie HI LUI THAOW MS7MS RAOW ADpliad Biosystems 1 Dynamic Range NanoFrontier elr NanoFrontier eLD TDC ADC Analog to Digital Converter ADC TDC
17. 100Vx15A 1 2 L 2 6 22 27 2C ME UN UME 0 65MPa 2 5L min 4 ESI 22 5L min man jh lel TETIL i 99 5OnL min 99 95 iomollIh QHUIm rm Et mt 01 In ON RONEN 2 LC ma 0 35MPa 10mL min B01 5 In 98 0 oz A B 98 2 Qmin 60 40 60min 2 B8 69 17Omin 99 9999 1 i PP 0 SH nanoLC 49 08 54 48 57 95 81 26 63 93 EK A NanoFrontier eLD MS _ _ 4S0kg 51
18. RU AO 1 Ja Fa AUy NE _A 105 8717 011 707 3343 06 4807 2551 082 221 4514 092 264 281 075 241 1591 092 778 3005 029 825 4811 087 814 991 098 863 8295 052 219 1683 RE http www hitachi hitec com science 03 3504 7211 284 14 03 3504 7211 8 50 11 50 12 45 17 30
19. LC MS MS Desorption Fragment Search Desorption Fragment Search Desorption ragmene Se Eee rt ker F ran ea lt hh 2 MS MS 3 MS IMS IMS _ EE 1 A MLu mi um LC MASCOT MS MS MS Auto MS MS IBA
20. MS3 Desorption Peak Mode MS MS Epigallocatechin gallate 152Da MS3 Desorption Peak Mode ETigmliocatehln dmllafa 57Da 199Da Epigallocatechin gallate IBA MS Eninallgcatachimn em El WI ET HI Tam mm ma Im el hr 1 i i LET CCrHkO J 1 FF nii 5 esornliom Fask iMordg EpIgallocalechIn gallate BA MS MS MS MS MS
21. 8 LC MS HPLC semi micro ESI APCI i ue ESI APCI J 0 4 am MS a ee E TI nano ESI nano LC nanoLC semi micro ESI APCI HPLC ELgge il semiamicro L 2100
22. MS MS IBA Information Based Acquisition Desorption Peak MS3 Target Exclusion MS MS MS MS Target Inclusion MS MS MS MS IBA
23. ie A Mass Navigator ll dat MS MS a en MS MS m m F 0 1 Te l 1 1 http biomkicojp do Pr OS HH mz pn mzData rmi netCDF Expo AE Yome rs i etc ur i ai 5 zn 2 4 Xome Mass Navigator 15 17 NEDO MASCOTT Matrix Science Ltd Xome
24. MSr LIT 2 q TOF q CID LIT q N LIT q q LIT 1 3 ITOF ESI TOF APCILTOF 4 QuECD ECD LIT ECD CID P5 a Cn MS NanoFrontier Ln NanoFrontier eLD
25. de 7oro MS MS ge novo Bioinformatics Solutions CR tn wwwinfocomicojp bio Xome Mass Navigator MS MS Xome Mass Navigator RE S PC Export a De novo 1
26. 1 83 583 9 653 34 528 28 5 NN B LC 85kg 064 063 56 054 0 752 C 20kg Na SCX ColUm 1 D D1 44kg D2 29Kg min E 90kg ES 5nL mmim A BDCHICN 50mg ml i EE AB 6 10 Gmin 07100 Ormin Exaocctn k UV273rm 31nlL mmj 4 20OnL min VS 5UnL mm ER SA 3 Cm 493594617 14642 554 L Angioteri5in T 5pmQl i 1 SBCX 00 m x BOmm Solyenti0 20 d0 75710D0mmM HCOONH Tt 2 iQHS 5G mx 15Omim sm0nMmin NN LL 200nL mln w 5On min ss ll lM WM WW ET Peak Area 5 A NN ew mm Peak Hal 1t 3 ML h jl Mi A MI UI 0 lnIIETT THT rin FL ma Tr mln nL mi Gi dr Tmml
27. 2 4333 _ 1056 4766 _1171 502H 1285 5963 CHsiNOio 1 1 xr HI 20 BXDected meass 158 11756 a 0 0005Da N EI MGT 1 H 11TUEg lim Elitet dt APD 1 119 246 1561 339 1881 480 2565 es BB 6 39389 942 31 1056 4745 1171 501 1285 5444 EE WO ee NanoFrontier eLD TOF MS NS2 Glu Fibringpeptide HB 000L4D2 expected mass Dal MS LIT TOF MS MS
28. Er 00m MnmliHh Tran O BmmLHX150mmL A O 155 In 296 0 196 In HB 98 0min 60730 BOmin i BSA Hatenllan Timg inln
29. lld Time IT Liquid Chromatography NanoFrontier ein 2 LC MS 2 sm a n 5 50nL min nanoLGZMS 7 100Vx5A
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