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1. MoFlo Astrios EQ EQs 23 100MHz 10 7 9 4 9 MoFlo Astrios Q EQs g 99 100 90 80 70 60 50 Purity 40 30 20 10 0 EE2. MoFlo Astrios EQ EQs ns FY 0 5mL 1 0ml 1 5ml 5ml Zmll 15mL 50mL R Theta CyClone INew Parameter MoFlo
3. 0 2m FITC lt 125MESF PE lt 110MESF Spherotech 8 Peak 488nm 100 000 70 000 gt 99 1 70um 60PSI 70 000 200 000 200KHZ CCD IntelliSort I Sort Rescuer Intellisort I 2 6 Clonele4 6 A41536C EE CyClone Il 1536 Cooling Aerosol Evaluation JIS K 3800 2009 7
4. Sorting Capability MoFlo Astrios EQEQs 6 Purify Enrich Single Abort Stream gt 99 CD123 Sort Purity CD11c Sort Purity CD11c Aborts Sample_70 G1 R2 Sample_34 G1 R2 Sample_73 G1 R2 10 105
5. MoFlo Astrios QEQs 99 1 0 0001 a ri ea RT Coke optical control eb a mw Hoechst muc FITC MoFlo XDP Astrios 1 singl e cell EpCam Alexa647 single cell sorting MM 0 0001 YY 1 proof L h t Control positive MoFlo Astrios 0 0001 RT PCR
6. R Theta CyClone 7 Ml R Theta CyClone AIN ll 1 3 AES Aerosol Evacuation System
7. Kaluza 1536 MoFlo Astrios Q EQs 1536 HH 1 1 0 Lc 10 Easy to Use MoFlo Astrios EQ EQs Fiber Beam Conditioning Optics FBCO 6 1 11
8. MoFlo Astrios EQ ECQs Service and Support 0120 826 7 03 6745 4705 9 00 17 30
9. Two Day 2 MoFlo Astrios MloFlo XDP VVeD http WwwwW Dc cytometry com 20 SR a y oF EX WWeb FCM VWeb http vww Dc cytometry com 13 MorFlo Astrios EQ EQs
10. 355nm 100mW 405nm _ 55mW 488nm 200mW 150mw 532nm 150mW 561nm 200mW 592nm 200mW 642nm 100mW EQ 200mW EQs 150mW PMTX2 PMT PMT PMT 51 EQS PMT1 50 70 100m 50 70 80 90 100 120 150 200um 8 0 5 mlL 1 0 mlL 1 5 ml 5 mL 7 mL 15 mL 50 mL 100MHz 0 01 1 23 8 388 608 32 4294967296 9 4 9 SO 32 20x20
11. MoFlo AstrioS EQ EQs geooew COULTER 1995 MoFlo MoFlo MoFlo Astrios EQ F MoFlo EQ Quinox aequus equal nox night EQUuinox ddle 7 40707ajiiyaS10 JaliuE FSC enhanced Forward Scatter 9 Astrios Perfect Fluidics Design
12. FBCO MoFlo Astrios QEQs 6 1 Fiber Beam Conditioning ptics FBCO hh MoFlo Astrios Q EQs a _ _ a _Core a MoFlo Astrios EQECQs PEIN BRN Cells 0 2um 9 eFSC MoFlo Astrios EQ 2 eFSC
13. 6 1 C MM A Ce ll 5 OO 6O QC IntelliSortI Astiros EQ EQs II
14. Ld BD LL SortIndex 4 2 pe o F 32333 0 0 wm lt K x 1 FE 1 TD EE EH tt Le Ce 2 3 2 1 Sortindex
15. MoFlo Astrios EQEQs CytoNozzle CytoNozzle NM MoFlo Astrios EQ Q EQs Viability Vitality Crystal sample In Sheath In Out Sample Sheath Delivery Sheath Sample Delivery h WY Nozzle Tip 8 50 70 80 90 100 120 150 200um 8 CytoNozzle CytoNozzle CCD IntelliSortI 70 100um
16. MoFlo Astrios EQ EQs BSC MoFlo Astrios QEQs NSF 49 3 BSC BSC SterilGARD Biosafety Cabinet A B C D E F G H HEPA WWHEPA BSC MoFlo Astrios EQ 5
17. i Fas CE pr ed MoFlo 60PSI i 9 1 1 28 09m Sec NV 0 82 u Sec Rn on 0 AM 2 4 4 0 MM MM iu Wh Te Puke widn 182 5 LA 5 5m Sec 3 96 u Sec I f EE 1 0 1 fe Fuke widh 199s 13um Cell size Purity Sorted Beads MoFlo CytoNozzle 0 20 000 40 000 60 000 80 000 100 000 120 000 Events Per Second e MoFlo 1 MoFlo 2 Sorting Performance R Theta CyClone MoFlo Astrios QEQs R Theta CyClone 1 4um 0 25um 5 1 1536
18. 2 0 2 30um Astrios EQs 1 eFSC 7 355nm eFSC MoFlo Astrios EQ EQs J oo eFSC 561 53 tog etgh 453 SC toe Heget 0 2 30m Digital 1 echnology
19. 0 04hm 3 MoFlo Astrios Q EQs 0 04urm 0 1m 3 99 9999 MoFlo Astrios EQ EQs 3 Optics Design Z 7 7 MoFlo Astrios EQ EQs 7 7 49 49PMT
20. 1 650 W x 770 D x 1 295 H mm 567Kg 450 W X665 D x 861 H mm 191 W x 457 D x 429 H mm 10 5Kg IO 50 60 2 Beckman Coulter Beckman Coulter CyClone MoFlo Beckman Coulter Inc A 14 MoFlo AstrioS EQ EQs 135 0063 3 5 7 TOC COULTER 2015 3 2000 L
21. 30m M1 MoFlo Astrios Multi Color MoFlo Astrios QECQs 7 FITC Phycoerythrin J Sorting Performance WBA abe WT Fengeihmm HLT mmd PE RE Lndigw ee ime ST Cbd a LT PE WT le FF H WF EO ET 6
22. c 510 510 5 03 10 c 10 ow wo 5 5 10 1 109 109 10 10 103 10 105 109 10 10 103 Dt 105 10 103 105 540 671 30 Height Log_Comp 540 571 30 Height Log_Cbmp 540 571 0 Height Log_Comp ourt Hst Regonl couk Nskl gon counk Hstl 100 00 547 100 00 282 S51 55 Total 326 1003D R7 323 99 5 Sort 8 5 Sort 99 08 L1 5 ml tube Purify 1 CD4 HLA DR CD11C 0 11 R2 50 ml Aborts Aborts of CD4 HLA N A N A conical tube fromL1 DR CD11C L1 5 ml tube Purify 1 CD4 HLA DR CD123 0 119 99 229 R2 50 ml Aborts Aborts of CD4 HLA N A N A conical tube fromL1 DR CD123 Astrios FQ EQs 99 7 MoFlo Astrios Q EQs 6 1536
23. Astrios Q EQ s QC QC QC Bio Safety amp MoFlo Astrios EQ EQs 3 10 3 a es MoFlo Astrios EQ EQs a JIS K 3800 2009 i
24. Astrios EQ MoFlo Astrios EQ5 5 10um eFSC 3 7 gt e AO2 a eFSC 10 a tm P3 ns m9 Ro 8 t Te 1 0 ry he 100 FSC Log Height 100 1 ml 102 103 10 88 FS 1 HeigHt Lng A Microparticles B Platelets C RBC D Lymphocytes E Monocytes Granulocytes 2 MoFlo Astrios EQ 200nm
25. ememti 1 1 TE Th a BT HE LI td 1 Er ET IL 7 6 UV 355nm 100mW 488 59 Lineage 640nm a B220 CD3 Gr1 mac1 Ter119 A APC Cy7 CD117 c kit 795 70 592nm DyLight594 CD48 620 29 561nm PE Cy CD150 795 0 Vasilis Toxavidis John Tigges amp Meritxell Alberich 532nm FCgR 576721 Jorda Hematopoietic Me Cell Sorting Without Compromise 7 Lasers Eight Colors Six Way Sorting A OO 0 A Novel Approach a Beckman Coulter Life Sciences White 405nm Pacific Blue Sca 1 448 59 Paper 1 NE 1 me I E Er Bsa TT TT ARUN EE TNT RE HH illll TT Hi HW RR ECan CM TER rn EE PhinFlio A Flim Ei EE er Cgmplete Sorting Package i 5 mm mm rd j pF Fk mt 2 ET Teakg MoFle Astrios
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