大豆中の粗蛋白質の定量
Contents
1. 1 AOAC 1g 1 AOAC TCMD HzOz2. S6 Tecator 16 1 HzOz 5 0 078 g N 58 12 1983 4mg HgSO4 3 CuSO4 5HzO 4 KsSO4 40 1 5g 5ml 2 30 NHs 1
3. 1 2 1 2 1 2 34 5 34 5 34 5 34 5 34 5 3 TCMD 1 HzOz 1 2 2 58 12 1983 OI 34 3 34 3 4 i
4. 20 6 4 SO 8 2 HgSO4 TCMD 20 420 C 40 ii
5. 1 HO g Im t IT1 Cg So 90 AOAC 1 10 7 221 15 el 9 AOAC 2 0 65 i 1 0 2 i AOAC 3 Kjel Foss 0 6C 15 0 75 12 15 10 _45 N 0 003 0 85 085 ss 0 005 a __ 0 128 0 17 NO 0P 10 1 20 mm N 0 9 1 25 N 0 015p 9 0 1 0 3 20 2 NO ai 0 2 0 4 20 1 27 esln TCMD 1 45 0 5 10 8 I N 0 003 0 005 0 003 0 005g Ii 1g 60mg
6. 8 100 mli TCMD Tecatox 1015 Digestion System 20 1 420 C 40 HzOz HzOzs 2 4 ml 8ml HzO HzOs HgO CuSO Se CuSO4 TiOs 4 3
7. HzOs 4 ml 8ml 8 ml HzOs 5 g 2 HzOz HzO DL g ml 1 4 0 33 5 34 0 34 6 31 0 33 3 a 3 4 34 6 34 4 33 9 33 5 34 1 8 34 9 34 8 34 7 34 8 0 35 0 35 0 34 3 34 8 5 4 33 2 36 5 36 2 35 3 8 35 2 35 2 35 2 35 2 a 10 ml KsSO 9 CuSO lt 5HzO 1 5 1 4 TCMD 5 g 10ml Hz202 8ml
8. 35 2 34 5 TCMD 34 3 35 3 20 HgSO4 TCMD TFCMD HzOs HzOz
9. 1 R B Bradstreet Chem Rev 27 331 1940 2 P L Kirk The Chemical Determination of Proteins in Advances in Protein Chemistry ed by M L Anson and J T Edsall Academic Press New York 1947 vol 3 p 139 3 1981 p 14 4 a W Horwitz ed Official methods of Anal ysis of the Association of Official Analytical Chemists 13th edition Association of Official Anajlytical Chemists Washington 1980 p 15 b ibid p 127 5 2 1980 p 518 6 1962 p 88 7 2 969 p 75 8 1976 p 81 9 25 25 1979 p 20 10 Tecator
10. m9 9 Nelson 1ml 0 33 0 5 g CuSO Se SeOCl2 Ti02 29 MnO4 HHC1O4 38 NH HzO2
11. 2Oz HzOs 5 g 10m1l 3 HzO2 HgO CuSO4 Se CuSO4 TiOs 4 CuSO4 KzSO4 9 CuSO4 5HzO 1 5g 5 g 3 1 g I II I IV 10 ml TCMD 4 4 HgO
12. Se CuSO4 Se CuSO TiO2 TiOs TiOz 3 Cmg a HgO 233 5 I 5 CusSO4 5H20 500 4 5 I Se 5 5 2 AOAC 5 I C S IV CT 38 4 1a 2 a 1 1 29 34 7 35 3 34 7 34 9 34 9 HgO CuSO 34 4 34 4 Se 33 9134 0 34 3 34 0 34 1 CuSO4 Ti0 33 7 34 0 33 6 33 4 33 7 34 3 34 2 34 3
13. 11 R B Bradstreet Anal Chem 29 944 1957 12 P A W Self Pharm J 88 384 1912 13 J M Concon and D Soltess Anal Biochem 53 35 1973 14 D W Nelson and LE Sommers J Assoc Off Anal Chem 63 770 1980 15 R B Bradstreet Anal Chem 26 185 1954 16 P L Kirk Anal Chem 22 354 1950 17 H W Gerritz and J L St John Anal Chem 7 380 1935 18 F M Stubblefield and E E DeTurk Anal Chem 12 396 1940 19 A V Barker and R T Volk Anal Chem 36 439 1964 20 C H Perrin Anal Chem 25 968 1953 21 R B Bradstreet Anal Chem 12 657 1940 22 P R Rexroad J Assoc Off Anal Chem 56 862 1973 23 P R Rexroad and R D Cathey J Assoc Off Anal Chem 59 1213 1976 24 P R Rexroad and G F Krause J Assoc Off Anal Chem 61 299 1978 25 G L Miller and E E Miller Anal Chem 20 481 1948 26 L G Hambleton and R J Noel J Assoc Off Anal Chem 58 143 1975 27 Tecator Applhcation Note AN 30 81 May 1981 Tecator AB Hogpanas Sweden 28 R L Shirley and W W Becker Anal Chem 17 38 437 1945 1974 29 S M Patel and A Sreenivasan Anal Chem 20 31 R Odland Assoc Off Anal Chem 55 984 63 1948 1972 30 W Glowa J Assoc Off Anal Chem 57 1228
14. 325 20s 2927 TCMD AOAC 1 2 50 1 5 71 112 ls 4 2 1 8
15. 06 38 Kjeldahl Determination of Soybean Protein Using a Block Digestor Keiko Kushioka 1883 Kjeldahl NH4 NHs Bradstreet Kirk 1900 6 25 1
16. NH NH NRN ft NIHs Gi NH4 TCMD 2 JE
17. Bradstreet Bradstreet 2 4ml 1g 7 3 g 9 0 g 17 8 g Selfi 28 2 35 3 19 0 1 g 4 7ml 1g 7 ml
18. HgO CuSO Se Ti02 HgO 22 24 30 3D CuSO4 4 TCMD TCMD 18 KsSO4 9 CuSO 5HzO 1 5 8 10 ml 420 C 40 HzOz
19. 1 g I HzOs g IA TEL Ti1 KSO CuSO Cg HgO Hg Heso so 0 7 i 25 0 65 15 0 75 12 15 10 LDL 0 TI IT0 1 19 1 1 44 1 91 60 60 6 120 36 4 100 Te Es 3 6 og 8 0 9 0 1 0 3 20 3 6 eee 4 2 7 0 3 10 4 5 0 5 10 8 886T zT 89 3 33 AO0AC 13 1 7 C CO2 HzO NH4
20. 2 HzOz TCMD HzO2 KsSO4 9 CuSO 5HzO 1 20 6 420 C 40 1g 2 10 ml HzOz HzOs 13 1 30 3g HzOs HzOz 5g HzO
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