CriterionTM トランスブロットセル 取扱説明書 - Bio-Rad
Contents
1.2.
3.
4. 0 IU 15 10000000 SDS 1
5. 1000000000000000002 000000000000000000002 00000000 1000000000000000000 7 0000000000000 03 IUUUUUUUUUUU UU IUUUUUUUUUUUUUUUUUUUU 1 1002 0000000000 1 000 0 0000 00 0000 0
6. 10 50V SOMA 750 950mA 000000 1200 600 10 50V 0 0 SOMA 300 500mA Table3 4 DNA RNA 00000005 TBE Criterion IUUUUUUUUUUUUY 1000000 000 3 3000000000000000 1200 300 10V 50V 100mA 750 950mA 000000 1201 600 20V 50V 100mA 300 500mA 32 000000000 1 000000 100000000000000000 DNA 00 0 0 0000000 00000000 OU 000 1000000000000000000000 2 000 200 200W IUUUUUUUUUUUUUUUUUUUUUU 00000 0
7. 000 0000 20 00 00000000000000000000000 000000000 1 00 00000000000000000000000 24 700000 100000000 0 45 IUUUUUUO IUUUU 00000 0 0 10000000 20 1000000000000000 IUUUUU 505000000000000 1000000000000 50500 5050 J 00 10000000000000 3 0 00 100 IUUUUUUUUUU 0000000000000 IUUUUUUUUUUUUU uu
8. DNA IUUUUUUUUUUU 3 100 0000 100 2 0000 1000000000000000000000000000000000000000 2 o gt gt section 5 00000000 5 1
9. 1251 Tris HCI Criterion 00000 400 0 Native 0 00 O O cytochrome pl 9 6 lentil Iectin pl 82 8 0 7 8 carbonic anhydrase pl 6 0 glucose oxidase pl 450 000000 10000 00000 00 xTris Glycinell 000 3300 00 0 0 0 0 0 0 0 0 00 0 Native PA 000 1000000000 00000000 0000 0 pHO0 0000000 10000000000000000000 JOU 00110 u 000000 1200 300
10. 00 000 10 UU 00 000000000000000 00000000000 00000 IUUUUUUUUUUUUUUUUUUUUUUUUU 000 0 000000 000000000000000000000000 100 JUUUUUUUUU IOU 000 IUUUUUUUUUUUUUUUUUUUUUUUUU UU IU U 000000000000000 1000 5058 000000000000000 5250 00 IUUUU 0000000 00 000000 IUUUUUUUUUUUUUUUUUUUUUUUUU 100 53 IOU JUUUUUUUUUUUU 0 000000000000000000000 00000 100 1000000000000000 U IOU 0 0 0 0 0 53 IOU PVDF 100 1000000 PVDF IU 3 00 10000000000000000 IU U 100
11. PVDF 2 000000000000000 O00000 PVDFQ O 20 0 j PVDF Polyvinylidene difluoride 6050000 000 1000000000000000 10000000000000 0 0000000000000 000 10000000000000 1000000000000 10000000000000 1000000000000 5051 000000000000000000000000 0 00000000000000 10 1 000000000000000000 000000000 000000 1000 100000000000000 0000 1000000000000 1000 100 50 Fast Green 000000000000 0 100 0000 0000000000 20kDa 100 0 24 000 OOOO 5550 000000000000000000 1000 SDS SDS 0 1 551 0000000000000000 00 52 1000 100000000000 00 10x ssc 1000000 0000000000000000 500520 0 000000000000000000000000000 100000000000000000 000000000000000 1000000000000 100000000 100000000000000 10900 00 10 0 DNAQ 0 RNA 2 00000000000000000 100000000 10000000000 100000000 00 0 000 00 00 0 0 0 0 Table5 1 1000000000000000000000 0000000000000000000000000000000000000000000000 0000000000000000000000000000000
12. Table3 19 0 340 00000000000000000000000000000000000000000 00000000000000000000000000000000000000000000000000 00000000000000000000000000000000000000000000000000 100000000000000000000000 Table 3 1 SDS PAGE 100 125 Tris HCl Criterion OO O O O E coli lysates O0 000000000000000 100 100 x Tris Glycine 00 0 3 3000000000000000 200 Methanol 10 Methanol 15 Ethanol 0000 0000 Table3 2 SDS PAGE 100V 300 100V 600 00000000000 1000 00010000600 O E coli lysates 0000000 000 Ethanol 000000000000000000000000000000000 CAPS 100V 300 100V 600 00000000000 00000000000 000000000 0100 0 Table3 3 Native PAGEQ J 0000000 12 5 Tris HCl Criterion 00000 E coli ysatesgOlQOOOOOOOOOOOOOOO 00000 IO UU 10 10003 3000000000000000 200 Methanol 10 Methanol 15 Ethanol 000000 100V 100V 100V 300 300 300 000000 100V 100V 100V 600 30 60
13. 700ml dd H20 dd 000000000 W 14 49 600ml dd H20 0 200ml 00000 10000 Towbin 250 0 000 192mM0 00 00000 10000 00 0000 pH 83 00000000000000000 100ml 10x T ris Glycine 100 100000 161 0734 OL 100000 16107711 5L 800ml dd gt 100770 BOO UU U 1 100 3031 000 14490 600ml dd H20 100 100ml 000000000 9007 000000000 20 1000 CAPS 10mM CAPS 3 cyclohexylamino 1 propane sulfonic acid 201 00000000 pH 11 0 dd H2O 800ml CAPS 221g 600ml dd H20 20070 00000000000 NaOH pH 1101
14. 18 tY uQ ka 20 Sectionl 00000000 11 0000 00000000000000000000000000000000000000000000 4 370 U 0 00000000000000000000000000000000000000 2000 000000000 0 000000000000000000000000 0 000000000000000000000000000000000000000000000000 00000000000000000000000000000000000000000000000000 00000000000000000000000000000000000000000000000000 000000000000000000000000000000 000 00000000 000000000 000000000000000 0000000000000000 000000 12 Criterion 0p 000000000000 21 8 11 8 15 cm 00000000 1 3L 9 45x13 84 cm 0000000
15. 000000 00000 00000 000 00000 4 3 100 000 114 16 5 0000000 94 15 0000 Criterion 0 200000000000 40 17 3 32 5 5 7 cm 173x14 3x3 2 0000000 17 3 12 1 3 2 13 000 Criterion 000000000000000 external DC voltage 00000000000000 100000000000000000000000000000000000000000000 10000000000000000000 00000000000000000000 100000000000000000 300Volts DC 100000 200Watts 50 C 0 IUUU IUUUUUUUUUUUUUUUUUUUUUUU IUUUUUUUUU 10000000000000000
16. 10 1000 0000 10mM CAPS 3 cyclohexylamino 1 propane sulfonic acid 10 pH 110 00000 CAPS 22190 600 dd 0000 NaOH pH 110700000 000000000 15 1000 CAPSOHOO0g 10mM CAPS 3 cyclohexylamino 1 propane sulfonic acid 15 0 0 OO 00000 pH 110 CAPS 22190 60077 dd 0000 NaOH pH 11070000 000000000 150ml 2 Native PAGE 25mM 0 0 0 192mM pH 83 100000 100ml 10x ris Glycine 100000 161073410 100000 1610771 5L 900ml dd H20 0000000000 100 3030 00000 14499 dd H20 300 0000 89mM 2mM EDTAQ pH 3
17. 000 PVDF IUUUUUUUUU I 1 00000000000 0 0000000000000000000000 IUUU 1000000000000000000 00000 00000 00 0000000000000 70 100000000000000000000000000000000000000 100000000000000000000 Section 8 Ordering Information Criterion 170 4070 00000000000000000000000000000000000200000000000059000 00000000040000 000000000000000000000000000000000000000000 170 4071 Criterion 0000000000000 0000000000000000000000000000000000020000000000005000 00000000040000 000000000000000000000000000000000000000000 170 4076 Criterion 00000000000000000 170 4080 Criterion
18. 0 0 0 EN61010 000000000000000000000 EN61010 19 0 0 000 EN6101010 0000000000 Criterion 10000000000000000 00 IUUUUUUUUUUUU 6000000 1010 100000 6 02 000000000000000000000000 Section2 000000000000 21 0000000000 00000000000000000000000 0 5 33 0000000000 4 0000000000000000 2100000000000 1500000000000000000000000000000000000 0000000000000000000000000000 3990000000 Criterion 0000000000000000000000000000000 b 0000000000000000000 gt 0000000000000000000000000000000000000000000000 0000000 000000000000000000000000000000000000000000000 0000000000000000000000000000000 000000000000000000000000 fd 0000000000 0000000 00000000 000000000 00000 00 000000000 00000000 0000000000 0000000 000000000000000 a 00000000 00000000000000000000000000000000000 0000000000000000000000000000 000000000000000000000000000000000000000000 0000000000000000000000000000000000000000000 OU 0 000000000000000000000000000000
19. 00 000 7 8 4 0 1000 162 0091 0 454 0 10 15 1000 162 0094 5 000000000000000000000 30cmx3m 0000 162 0095 02 000000000000000000000 7 84 1000 162 0097 0 24 000000000000000000000_30 3 0000 162 0174 0000 3000 000000000 7 84 1000 162 0175 0000 3000 00000000 10x15cmf 1000 162 0186 7 8 4 0 1000 162 0180 10 15 1000 162 0184 OOODOOOPVDFOOOOOOO00 26x330cm OOOO 000000 000 161 0734 10 161 0771 10 000 00000 510000 161 0774 20xSSC 1L 161 0775 20xSSCO5LO 000 Section9 OOO So NOM F WN NNN N N N N N N O ON Uu o N U Q N 29 30 31 32 33 34 35 36 37 38 39 40 41 Southern E M J Mol Biol 98 503 1975 Alwine J C Kemp D J Parker B A Reiser J Renartj Stark G R and Wahl G W Methods Enzymol 68 220 1979 Thomas P S Proc Nat Acad Sci 77 5201 1980 Seed B Nuc Acid Res 10 1799 1982 Renart J Peiser J and Stark G R Proc Nat A cad Sci 76 3116 1979 Bowen P Steinberg J Laemmli U K and Weintraub H Nuc A cids Res 8 1 1980 Towbin H Staehelin gordon j Proc Nat A cad Sci 76 4350 1970 Bittner M kupferer P and Morris C R Anal Biochem 102 459 1980 Stellwag E J and
20. 30 129 100000000000000000000000 0 30 50V 1000000000000000 U V cm U U U 0 0 IU 10000 pHo a 00 00 JUD 1000 000 b IUUUUUUU UU 1000 SDS SDS 10000000000000000 0000 000000 00000 00000 10000000000 0000000 00000 00 10000000000 000000 850 10070000 100000000 V cm 1000000000000 00 10000000000000000000000000 0000100 SDS 0 IUUUUUUUUUUUUU SDS 100000000000000000 10 505 10000000000 5250 000 00 505 0 2 000000000000000000 100000000000000 SDS 00 00 2 00000000000000000000000 00 00 0010 100000000000000000 Sections 0000000 0 IUUUUU 10000000 IUUUUUU 10000050000 PVDFQ 0 1000000 162 0174 1620175 162 0176 162 0177 0 0 0000 PVDFO l 162 0186 162 0180 162 0181 162 0182 162 0184 00 42
21. 16107331 0 LO OU 100ml 10x O00 16107701 5L 900ml dd HzO 0 00 00 EDTAQ 40mM U 1mM EDTA 20ml 50x 100000 0 LI I 100000 161 0773 O00 980ml dd HzO section4 0000000000 41 00000000 00000
22. 00000000010 170 4085 0000000000 500 9 5x15 2 cm 170 4086 00000000040 9 5 15 2 170 4089 0000 00 0 0000 0 165 1279 OOOO 000000 000 162 0212 0 2u mp 0000000 000000000000000 7 8 5 20000 162 0213 0 24 mp 0000000 000000000000000 7 85 50000 162 0232 0 24 mp 0000000 000000000000000 85 135 20000 162 0233 0 24 85 135 50000 162 0214 0 454 M 7 85 20000 162 0215 5 0000000 000000000000000 7x85 50000 162 0234 5 0000000 000000000000000 85 135 20000 162 0235 045 85 135 50000 162 0238 00000000 000000000000000 85x13 5 20000 162 0239 00000000 000000000000000 85x13 5 50000 162 0236 85 135 20000 162 0237 85 135 590000 162 0115 O45umD0000000000000000 30cmx3 5m 1000 162 0114 0 45 0 15 92 1000 162 0145 0 454 0 0 0 0 7 84 1000 162 0148 0 45um0 0000000000000000 115 16 1000 162 0112 0 24 mp 0000000000000000 30cmx3 5m 1000 162 0146 0 24 0000000000000000 7x8 4cml 1000 162 0147 0 2 00000000000000000 13 5 16 5 1000 162 0090 0 454 0
23. 00000000000000 100000000000000000000 0000000000000 8 Table3 19 0 3400000000000000 120 000000000000 20 00000000000 100000000000000000 120000000000000000000000000 IUUUUUUUUUUUUUUUUUU UU 0 0 0 UU UU 0 00 0 0 0 00 00 0 3 00 10000000000000 9 00000000000000000000000 100000000 59 00000000000000000000 OOOO 100000000000000000000000 IUUUUUUUUUUUUUUUU 00 33 0000000 000 10000000 0000000000 100000000 Criterion 000000600 1000 1 3 0000 3001 0000000 01 60 000000000000000000000000000000000 IO OU 0 pH 2 00000000000000000000 10000000000000000000 10000000000000000 U 000000 000 000000 00000000000000000000000000000000000000000 000019 0 0000000000000000000000000017 0 00000000 00000000008 0000000000000000000000000000 1 SDS PAGE 200 0000000 25mM 0 0 0 0 0 0 200 0 0 00 0 0 0 100ml 10x T ris Glycine 100 100000 16107341 1000 16107711 SLO 000 pH 8 3
24. 000000000 0000 000 9 cml 00000000 80 100 00000000 0 24 mI 000 0000000000000 00000000 00000000 24 00000 0000000 0000000 PVDF 150 160 OOOOOOO PVDF 170 200 Section6 0 00000000000000000000000 0000000000000000 0000000 1000000000000000000000000000000000000000000 00000000000000000000000000 000000000000000000000000000000000000000 0000 0 0 0 000000000000000000000000000000000000000000000 0 00000000000000000000000000000000000000000 Section 7 00000000000
25. 0000000000000 0000000000000000 000000000 000000000000000000000000000 00000000000000000000000 BIO RAD 100 00000000 D U 00005000 000000000005450 00000000 0000 000000 00009000 0000000000655 0000 77000 000000 00009000 0000000509 000000 77000 00000 0005000 00000000005050 000000 000000 00009000 00000000005005 MIDORID 0 77000 00000 0005000 000000000005050 0000000 21000 0000000000 200 005000 FAXO 00 00000 8000 005000 000 0000 8000 005000 000 0000 9000 005000 00 00000 9000 005000 000 0000 8000 005000 000 0000 8000 005000 00 00000 8000 M3128 0303 http discover bio rad co jp
26. 0000000000000 0 0000000000000000000000000000000000000000000 0 0 00000000000000000000000000000000000000000 0000000000000000000000000000 00000000000000000000000000 f 000000000000000000000000000000000000000000000 000000000000000000000000000000000000000000000 0000000000000000000000000000000000000000000000 0000 00000 a 1 0 0 0 0 0 0 0 0 9 0000000000000000000000000 2 00000000000000000000 0000000000000000000000 0000 00 200000000000000000000000000000000000 00000000000000000 i 00000000000000000000000000000000000 22 00000 a 0000000000000000000000000000000000000000000000 0000000000000000000000000000000000000 b 00000000000000000000000000000000000000000000000 0000000000000000000000000000000000 2000000000000 0000000000000 0 Sections 000000000 000000000000000000000000000000000000000000000 23 000000000 0000000000000000000000000000000000000000000000000 00000000000000000000000000000000000000000000000000 00000000000000000000000000000000000000000000000000 00000000000000000000000000000000000000000000000000 000000 Section 3 0000 31 0000000000000000
27. 000000000000000000000 00000 0010 200 1000000000 3 00000000 000000 0010 3 4 0000 UU Ua a 1 00 1 2 000000000000000000000 10000 10000000 000000000000000000001000000000000 Criterion 0000000000000000000000000000000000 IUUUUUUU 5 0000000000 Criterion 0000000000
28. Criterion 0000000000 00000 Catalog Numbers 170 4070 170 4071 Section 11 12 13 Section2 21 22 23 Section3 31 32 33 Section4 41 42 Section5 51 52 Section6 Section7 Section8 Section9 Section10 1 ae aid 1 0 2 2 BREESE sa 3 E YR Hn O atn a 3 5 BWR EW RS nia NR 5 6 ds 6 AN 7 8 NN E 11 11 DNAD 12 aa dela 13 CON FA NF DG anes 13 OO OA OOT 13 TE dea 15 a 16 Ordering Information
29. Dahlberg A E Nuc Acids Res 8 299 1980 Kutateladze T V Axelrod B D Gorbulev V G Belzhelarshaya S N and Vartikyan R M A nal B iochem 100 129 1979 Peudelhuber T L Ball D J Davis A H and Garrard W J Nuc Acids Res 10 1311 1982 Danner D B Anal Biochem 125 139 1982 Bio Rad Technical Bulletin 1110 Zeta Probe Blotting Membranes 1982 Holland L J and Wangh L H Nuc Acids Res 10 3283 1983 Syminton J Green and Brackmann k Proc Nat Acad Sci 78 177 1981 Reiser J and Wardale J E ur J B iochem 114 569 1981 Burnette W N Anal Biochem 112 195 1981 Legocki and Verma 5 Anal Biochem 111 385 1981 Lin W and Kasamatsu H Anal Biochem 128 302 1983 Andeson N L Nance S L Pearson T W and Anaderson N G Electrophoresis 3 135 1982 McLellan T and pamshaw J A M Biochem Genetics 19 647 1981 Gibson W Anal Biochem 118 1 1981 Hower J G and Hershey J W B J Biol Chem 2566 12836 1981 Erickson G Minier and Lasher S J Immun Meth 51 241 1982 Tsang V C W peralta J M and Simons A R Meth Enzymol 92 377 1983 Gershoni J M and Palade G E Anal Biochem 124 396 1982 Gershoni J M and Palade G E Anal Biochem 131 1 1983 Syminfton J Two Dimensional Gel Electrophoresis of Proteins Methods and App
30. lications Celis J and Bravo R eds Academic Press 1983 Andrews A T Electrophoresis Theory Technipues And biochemical and clinical application 2 nd ed Clarendon Press Oxford 1986 Beisiegel V Electrophoresis 7 1 1986 Bio Rad Laboratories unpublished Gershoni J M in Advances in Electrophoresis Vol 1 Chrambach A Dunn M J and Radola B J eds VCH Weinheim in press Gershoni J M in methods of Biochemical Analysis V ol 33 Glick D ed Wiley New Y ork in press Bjerrum J and Schafer Nielsen C Analytical Electrophoresis J Dunn ed V CH Weinheim 315 Dunn S D Anal Biochem 157 144 1986 Zeta Probe instruction Manual Bio Rad Laboratories 1988 Polvino W J Saravis C E and Cook R B Electrophoresis 4 368 1983 Bio Rad Laboratories Biotin Blot Total Protein Stain Instruction Manual 1985 Larochelle W J and Froehner 5 J Immunol Meth 92 65 1986 Szewcyzyk B and kozloff L M Anal Biochem 150 403 1985 Perides G Plagens U and Traub P Anal Biochem 152 94 1986 Section 10 0 0 00000000 1 0000000000000000000000000000 00000000000000000000000000 0000000000000 000000000000000000000000000000
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