B1-RD1
Contents
1. gt PBS PBS 2 8 C NBS 2 BSA 0 196 0 1 gt 1 5L 5 L 195L PBS 1 PBS PBS 6603369 12. Fc 10 15 1mg mL
3. MSlgG2a RD1 1 296 29 T T Harvard Medical School Dr S F Schlossman Coulter Immunology
4. 90 8596 Ficoll Paque Ficoll Paque 5 Ficoll Paque
5. B1 CD20 488nm RD1 FS 90 SS Tt 10 11 EDTA 6
6. gt PBS PBS PBS 2 lt 8 NBS 296 BSA 0 19 0 19 PBS PBS 0 19 2 lt 8 gt 1 1 2 100L 3 200L MSlgG2a RD1 200L 4 45 5 a b
7. B1 RD1 B B lgG B1 RD1 B CD20
8. CD20 B Fc B1 H299 CD20 lg B BALB cJ P3 NS1 AG4 lgG2aH zL B1 RD1 RD1 Phycoerythrin PE RD1 0 5 1 5 486 lt 580nm 568 590nm 1 0 5mL 0 296 0 01M 0 15M 0 196 B B B1 CD20 B
9. B1 CD20 B B1 CD20 B1 B Pokeweed mitogen PWM B1 3 9 CV 7 1
10. T B 3 Pan T Pan B T B pan B CD20 B1 B T B
11. 3 MslgG2a RD1 B1 RD1 6602888 100 0 5mL 4 Propidium lodide Calbiochem 53705 0 01mg mL 0 05mg mL 5 Acridine orange Baker A366 3 0 005mg mL 6 Ficoll Paque Pharmacia 17 0840 03 EDTA 1 100 L 3 10 x 103 mm 10 x 10 mm3 3x107mm PBS 20 25 C 6 a
12. 3 20 25C 4 B1 RD1 B1 RD1 6603446 100 0 5mL Stashenko P Nadler LM Hardy R and Schlossman SF 1980 Characterization of a human B Iymphocyte specific antigen J Immunol 125 1678 1680 2 Reinherz EL Haynes BFNadler LM and Bemstein ID eds 1986 Leukocyte Typing II New York Springer Verlag Vol 2 p 8 20 3 Morimoto C Reinherz EL Nadier LM Distaso JA Steinberg AD and Shlossman SF 1982 Comparison of T and B cell markers in patients with Sjogren s syndrome and systemic lupus erythematosus Clin Immunol Immunopathol 22 270 4 Koepke JA and Landay AL 1989 Precision and accuracy of absolute lymphocyte counts Clin Immunol Immunopathol 52 19 27 135 0063 5 7
13. 6603446 2009 11 2007 2 16300EZY00801000 877429 B B1 RD1 IN ww RD1 Phycoerythrin PE 1 2 5ug B1 CD20 30 35kD CD20 B T
14. 4 Ficol Paque 5 2 8 C PBS 2 8 C 400 450 xg 8 6 PBS 7 2 lt 8 C 400 lt 450 x g 4 8 PBS 9 2 lt 8 C 400 lt 450 x g 3 10 PBS 1 x 10 mm 1 x 10 mL 11 a b 9096 2 a 12 1 x 10 1x10 mm 1mL 13 2 8 PBS 2 8 C 400 450 x g 4 14
15. 0 196 3 4 5 6 IO 1 28C 2 B1 RD1 2 8 C
16. 2 Ficoll Paque 1 12mm x75mm 2 Ficol Paque 1 x 10 1 x 10 mm 1mL 3 2 8 C 400 lt 450 x g 4 4 200wL MSlgG2a RD1 200L 5 2 8 30 6 PBS 1mL 2 8 C 400 450 x g 4 7 5 2 8 0 5 1mL PBS 9 EPICS Cytomics 3 Ficoll Paq
17. a 2mL 5 15 b PBS 2 3mL 400 450 x g 5 PBS 25 1mL 30 2 250 kL 6 PBS 2mL 7 400 450 x g 5 8 6 7 9 0 5 1mL PBS 10 EPICS Cytomics
18. 0 05mg mL Propidium lodide 3 1 15 PBS 2 8 C 400 lt 450 x g 4 16 15 17 859 b 12 25 000 1 x10 gL 25L 13 Propidium lodide 0 01mg mL 10L 14 30 Acridine orange 0 005mg mL 10L 3 15 16 100 859 6
19. 10 3 4 11 PBS 200L EPICS Cytomics 1 2 Ficoll Paque 3 RD1 PE Log 1 CD20 Bh MSlgG2a RDI CYTO TROL 6604248
20. 500mL pH 7 2 0 2 0 196 2 1 2 1 6603152 300 PBS 24mL 1mL 9 2596 2 1L 8 26g 1 0g EDTA4Na 2Na 37mg pH 7 2 7 4 1 1 2 30 2 10 15 20 30
21. gt 10 x 10 mm 10 20 x10 2 20 30 x10 3 30 40 x10 5 40 60 x103 6 2 4 60 100 x103 100 200 x103 10 20 b 3 x 10 mm 1 25 C 500 x g 5 2 3 4 LH 700 5 10x10 mm 1 100L 2 Ficoll Paque 1 4mL PBS Ficol Paque 4mL 1 3 2 8 C 400 lt 450 xg 30
22. TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC FF ECKMAN COULTER 6603446_0911 4 4
23. ue 1 1 x 10 200L Ficol Paque 96 200L 2 2 8 C 400 450 x g 5 3 4 5 200L MSlgG2a RD1 200 L 6 2 8 C 30 7 2 8 C 400 450 x g 5 8 3 4 9 PBS 200L 2 8C 400 450 x g 5
Download Pdf Manuals
Related Search
B1 RD1 b1 r11 b1 rda b1 rdi bird by bird b1rd ultralight b1rdgen_final b1rd cybertalents b1 radio b1 radio live b1 rads 1