カラムを長持ちさせるコツ
Contents
1. Fig 1 25 40 L column2 ODS 500 10 110 EO TT TT e 25C 90 40 Durability test conditions Mobile phase CH3OH 20 mM Boric buffer pH 9 10 90 Flow rate 0 2 mL min Analytical conditions Column L column2 ODS 5 um 2 1x150 mm C18 Mobile phase CH3OH 25 mM Phosphate buffer pH 7 80 20 Flow rate 0 2 mL min Temp 40 Amitriptyline 00 100 200 300 400 500 Time Hour Fig 12. 10 10 PA 4 E lt lt 5 4 92 100 L column 1096 3 KS
3. ON OFF 0 5 um 1 0 5 um 3 um 1 10 2 Fig 2
4. L column 5 um 1 5 3 0 mm 2 0 mm 4 0 6 0 mm 4 0 mm or 4 6 mm 10 20 mm 10 mm Fig 3 4 Fig 4 HPLC 0 10 20 30 Time min Analytical conditions Column L column ODS 5 um Column size 4 6 mmx150 mm Mobile phase A CHsCN B HO A B 0 100 100 0 0 30 min Flow rate 1 mL min Temp 40 C Detection UV 210 nm Fig 4
5. THF 6
6. 9 CERI 1009 pH
7. 2 2 lt 3 5 6 L column 7 10 4 6 mm I D x150mm L 2 5 mL 8
8. ODS pH lt pH 2 7
9. 50 50 L column2 ODS 5 um 4 6 mm I D x150 mm L 50 50 1 mL min 20 2 3 ODS 5 10096 1 mL min 20
10. 3 um 2 um Fig 5 4000 80 L column CO Durability test conditions Column L column2 ODS 2um Column size 2 1 mmx 50 mm Mobile phase CHsCN H gt O 60 40 Flo
11. 10 7 Fig 7
12. A 0 Fig 8 Time min Time min 4 4 27 87 2 0 2 4 6 8 10 Time min 0 4 6 Time min 8 10 Analytical conditions Column C18 5 um Column size 4 6 mmx150 mm Mobile phase CHsCN H gt O 60 40 Flow rate 1 mL min Temp 40 C Detection UV 254 nm Sample 1 Uracil 2 Benzene 3 Toluene 4 Naphthalene Fig 8 Chemicals Evaluation and Research Institute Japan http wWww cerl or p _ CERI e mail chromato ceri jD TEL 0480 37 2601 FAX 0480 3 2521 345 0043 1600
13. 2013 07
14. Fig 7 8 Fig 7 47000 N 4 8500 N 4 17000 P 17 5 Mpa P 17 0 Mpa P 13 0 Mpa 1 1 I i 0 4 8 0 4 8 0 4 8 Time min Time min Time min Analytical conditions Column L column2 ODS 3um Column size 4 6x150 mm Moble phase CHsCN CH gt O 60 40 Flow rate 1 mL min Temp R T Detection UV 254 nm Inj vol 0 5 uL Sample 1 Uracil 2 Benzene 3 Toluene 4 Naphthalene Fig 7 Fig 7
15. LC Technical Report ODS Keywords
16. pH 9 EE MN SEM 3 5 um 2 um 3 Um 0 5 um 2 um 0 5 um Fig 2 L column 1 JIS K 0124 2011 8 1 4 2 2 JIS K 0124 2011 8 2 8 2 1 1 3 CERI Ye 40 ct Fig 3 Fig 3
17. w rate 0 6 mL min Temp 40 C Inj vol 0 5uL Naphthalene 9 0 L column 0 1000 3000 4000 2000 Fig 5 L column Fig 6 L column 4 2 654 N 4 18570 S 4 1 128 P 25 5 Mpa LL 1 2 0 1 2 3 Time min Time min Analytical conditions Column L column2 ODS 2 um Column size 2 1x100 mm Moble phase CHsCN CH gt O 60 40 Flow rate 0 4 mL min Temp 40 C Detection UV 254 nm Inj vol 0 5 uL Sample 1 Uracil 2 Benzene 3 Toluene 4 Naphthalene Fig 6 4 CEKRI
Download Pdf Manuals
Related Search