概要 必要な試薬と装置 操作手順 Lyse/No
Contents
1. 3 4 1 XPBS 34 5 IL 1 Clinical Applications of Flow Cytometry Quality Assurance and Immunophenotyping of Peripheral Blood Lymphocytes Tentative Guideline Villanova PA National Committee for Chnical Laboratory Standards 1992 NCCLS document H42 T 2 Procedures for the Collection of Diagnostic Blood S2. 5 Vortex 6 FACS Lysing Solution 10x BD 349205 FACS 7 FACS5 EDTA3K 20 55
3. 33 1 50L FALCON 1 x75mm BD 3G52058 GO0 L 20 25C 15 3 10 FACS Lysing Solution 450 L 4 15 30 5 FACS 1 EDTA3K BD 10 FACS Lysing Solution BD FACSG 3 10 FACS Lysing Solution
4. Lyse No Wash FACS Lysing Solution MultiTest TriTEST TruCOUNT 1 EDTA3K BD 367660 FALCON 125x75mm BD 352058 3 4 BD MultiTEST TriTEST
5. pecimens by Venipuncture Approved Standard Villanova PA National Committee for Clinical Laboratory Standards 1991 NCCLS document H3 A3 3 Landay AL Muirhead KA Procedural guidelines for performing immunophenotyping by flow cytometry CHn Immunol Immunopath 1989 52 48 60 4 Nicholson JKA Rao PE Calvelli T et al Artifactual staining of monoclonal antbodies in 34 two color combinations is due to an immunoglobulin in the serum and plasma Cytometry 1994 18 140 146
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