血球成分より抽出した RNAからのMajor bcr-abl mRNA
Contents
1.2. 4 Major bcr ab7 mRNA copies assay RNA Major bcr ab7 mRNA copies ggRNA R NA 0 5zgRNA 100copies assay 200copies un gRNA 2zgRNA 100copies assay 50copies gRNA CML 15 L 25L RNA 50L 65C 10 42 C 10 42 C 60 100 ux L 65 C 30 300L 65 C 15
3. RS05T1 Major 2008 2 2 2006 2 1 21500AMY00011000 bcr ab mRNA DNA FR Amp CML RNA Major c 2 mRNA 1 2 3 1 10 DNA FR Amp CML DNA FR Amp CML
4. 2 2 5 3 2 5 1 5 4
5. 1 2 8 80 20 2 1 2 1 50 1 50 1 Frank Gonzales et al Application of transcription mediated amplification quantification of gene sequences Gene Quantification 189 201 1997 2 50 310 314 1992 3 Q amp A 66 67 1996 4 L 3 21 1992 5 14 127 142 1995 6 10 252 262 1999 7 Junia V Melo et al The diversity of BCR ABL fusion proteins and their relationship to leukemia phenotype Blood Vo1 88 No
6. 80 20C 50 CML 8 Pit 1 5mL 1 DOGn 1 7 9 ICML RNA 0 9mL 1 10 CML 0 9mL 1 1L RNA Major 2cz g2 mRNA G BAND R NA Major bcr ab7 mRNA RNA 1 TMA Transcription Mediated Amplification RNA RNA
7. Ds 50HPA 10 0xL 6 5 1 3 0 300 6 5 1C 15 10 HC 1 2 RLU Relative ight Unit
8. wr 28 50 1 CTP GTP UTP 0 75mL 2 dATP dCTP dGTP dTTP 2 NOMU 1 5mL 1 MMLV 3 1 5mL 1 4 12mL 1 5 6m L 1 6 15mL 1 CML 1 7 6mL a DNA FR Amp CM
9. mm ww ss or 4 5 TMA Transcription Mediated Amplifcation HPA Hybridization Protection Assay CML 3 5 I 12X75mm 2 CML 1 4 R NA
10. 1 EDTA 2Na _ 6 2 8 8 2 72 R NA ON 59 2 R NA 9 3 2 1 RNA 250ng K562 CML
11. DDODIOD OIDODR 4 2 500L 750gL 900xL O 950L 4 50zL 100gL 400zL 6 5 500zL 750L 900zL O O POP 5 A De FD 2 1 15 25 100pL 200 300pL 9 2 R NA 1 5mL O O
12. Ds 50TMA Ds 50HPA 3 1 1 2 3 4 5 EDTA 2Na 2 8 RNA RNA 7 2 R NA RNA QIAGEN QIAamp RNA Blood Mini Kit RNeasy Mini Kit RNA R NA R NA RNA TE RNA TMA Transcription Mediated Amplifcation
13. DNA RNA T 7 R NA RT DNA cDNA RT RNase R NA cDNA RT DNA DNA RNA RNA Pol RNA RNA DNA RNA 1 T7 mm RNA mm RNase DNA DNA a T7 DNA T7 yg BNA Ni mm DNA YRNA DNA neco RNA RT MMLV RNA pol T7 RNA De y 1 TMA 2 HPA Hybridization Protection Assay RNA
14. 7 RNA 50 L copies assay 50 Ds 50TMA KN TR TMA HPA i so 5 3 20 1 CML AML 10 ALL MDS 2 6 1 6 50zL assay copies assay 0 n 17 1 20copies assay Major 6cr ab mRNA
15. I FN Dry Tap Gen Probe TMA HPA Major bcr ab7 mRNA TMA HPA
16. 4 copies assay 1 1 1 TE 0mmo1 L Tris 1mmol L EDTA pH 8 0 Tris EDTA 0copy assay 4 7LogRLU 2 CML RNA TE 2500copies assay 6 5LogRLU 2 100 500 1000copies assay 1 2 2 3 100 1000copies assay 3 CV 10 3 R NA Major 2cz g 7 mRNA 20 2500copies as say 2 CML IF
17. DNA RNA RNA DNA 2 Major bcr ab7 mRNA R NA AE RNA DNA RNA DNA T A c C N i Ke G A A G A
18. 1 HTIV HBV HCV 2 2 8 72 R NA RNA 3 3 2 1 2 3 1
19. CML 9 5 Philadelphia Ph 30 ber ab7 Ph 20 9 9q34 22 22q11 t 9 22 q34 q11 10 22 2cz gZ 9 q34 ce ab7 22 ql1 cr n 20 n 20 bcr ab7 m R NA oc g27 145kDa tyrosine kinase 2cz g2 190kDa 210kDa tyrosine kinase CML
20. o 10 0 Major 2c 6 5 1 3 0 3 AML ALL 5 30 Ph lt CML 30 0 L 6 51C 15 4 IFN mRNA 1 0 RLU Relative Light Unit 5 FT 2 RNA 2 g 7
21. 3 1 CML 15uL RAN NN RM 25zL 3 5 3 CML 2 CML RNA 80 20 42 X1 65 X2 O 3 2 O O 1 oO HC O 2 O O 3
22. 7 100 Chronic Myelogenous Leukemia CML CML 30 90 5 0 0 5 1 1 0 2 5 80 1 9 CML 5 70 CML 1 0 0 0 0 60 NAP B 12 50 CML Chronic Phase CP Accelerated Phase AP Blastic Crisis 40 BC 3 4
23. 1 W 1 1 i 0 Ee i a CML 562 CMLCML BO 2 1 750 CML Laz221 ALL Pre B blast 1 1 5mL BALL 1 ALL B cel1 MOLT4 ALL 3 ALM 16 ALL L id NIR RAR En 2 a SEA 6 5 5 10 CCRF CEM ALL T cell HPB MLT ALL T cell 4 ML3 AML 5 HL6
24. 1 IFN 1 IFN 1 2 1 2 3 M 1
25. amp SS a G DNA 2 HPA 7 1 gi 57
26. 12mmx75mm O O 6mL O O Ds 50HPA lt 2 EPS 3 2 5 7H 600eeT 5 NN 8 MM i NN NNN 4 TR FT gt 5 0 TOP a 6 RNA ENA RNA
27. 20 42 1C 5 HPA Hybridization Protection Assay 10 0 L 6 5 1 30 30 0xL 10 20 65 1 1 5 10 HC 1 2 RLU Relative Light Unit
28. 50z 1 4 RNA MDS 9 CMMoL H CML 3 5 40L Atypical CML 2 ET 1 2 0 0 ALL 2 6 5 1 1 0 AML 6 R 3 1 gt 3 4 2 1C 10 5 25 mL CLL 1 4 2 1 6 0 2 minor 2c 2 2 mRNA o _ 2
29. R NA CML 1 5 257 200zL 1 4 RNA 50L 6 5 1 1 0 6 5 1 4 2 1C 10 42 1C 2 5 42 1 6 0 6 0 2 8 C 2 20 C 1 2 8 C 5
30. Major bcr ab7 mRNA G BAND CML 1 IFN 2 3 Minimal Residual Disease MRD Donor Lymphocyte ransfusion DLT 2g 2 ZZ Major 6cr ab minor o 22 2 CML Major a6 Major bcr ab mRNA Major 2c 22 mRNA
31. 7 CML G BAND Ph FISH bcr ab1 IF N copies assay 6 CML G BAND FI SH
32. 10 CO Log RLU 2 1 3 7 5 70 6 5 6 0 5 5 5 0 4 5 4 0 3 5 0 20 50 100 500 2500 Major bcr ab mRNA copy copies assay 4 DNA FR Amp CML RNA RNA 1 2 3 3 1 1 2 RNA 3 RNA Ds 50TMA Ds 50HPA 4 5 TMA Transcription Mediated Amplification Ds 50TMA Ds 50TMA CML 3 5 12X75mm 1 4 RNA 50z
33. Ds 50TMA 1 4 RNA CML 3 5 40 200L 6 5 1C 10 42 1C 10 25 42 1C 6 0 6 0 2 8 2 20C 1 2 8 C 5 2 0 42 1C 5 HPA Hybridization Protection Assay Ds 50HPA
34. N 10 000 7L G BAND Ph 8 5 0z as say Amp CML G BAND 2500 2000 n 63 r 0 753 Y 96 343X 52 117 1500 1000 500 0 10 20 30 40 50 60 70 80 90 100 G BAND Ph1 Chromosome 8 G BAND 3 1 IFN 1 IFN CML 2 3 1 Major bcr g27 mRNA 2 2
35. 0 A L CML RNA TE 937 Ns rr 1 4 SKMES 1 HT1080 Fibrosarcoma 5 X10 SS 5 Hela 5 CML RNA _ SKBR 2 5 X 10 copies assay 50nL TEGO a 2 0 5 100zL TE 900zL lt SW 620 4 iO 4 1007L TE 400pL AML ALL Ph 0 0 3 100zL TE 400zL coples assay 2 0 copy assay 4 EE 1 uh TE 2 eR 2 6 CML 2 a CML
36. 7 2375 2384 1996 131 G Medical Technology 368 369 1988 CO ul 9 FISH CML 34 6 512 17 1997 10 IL 1 13 18 1996 EL 0120 292 832 FAX 03 5695 9234 2 62 5
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