過剰増殖性細胞と関連した抗原のような、抗 原に
Contents
1.2.
3. 11 1271 Mc E ie _ 4 5 0000 70
4. PTA 5412 0 5411 0 0000000 0000000000000 000000000 RM4 PTA 5412 0 5411 0 1 00000000000000000 00 0 0 0000 UU LU JJ JU UU UJU HU 0000 0 0 Ro 0 0 0 0 0 0 00000000 00000 0000 0 1000 AgRM4 AgRM 0 AgRM 0 RM 0 0
5. D 100 0 0 0 000 00000000000 0 00000 HHHHHHHHHHH Rm0 000 1000 0 10000000000 0 0000 10 30 0 30 5 50 10 7000 00 Fab Fab FvO F ab gt FV HHHHHHHHHHH 0 HHHHHHHHHHH RM 000000 U LU U JJ UU 0 0000030 LU 1000 50 60 70 75 80 85 90 95 96 97 98 l 100 JJ UU 0 0 0000000 100000000 0 000 0 100 000000 1000
6. CEA 10 2 MJC 40 p530 0 MAGE H O O O MAGE MART 10 0 gp100 0 0 Tn sTn Thonpson Fri edenr ei ch 2 B hCGB 0 HER2 neu PSMA PSA H 0000 0000 00000 00000 0000 100 100000000 NKO BH 1000000000 0 0 11 20 1 1L 18 IL 30 IL 60 IL 7 0 0000000 0 0 GMCSF 0 0 11 120 TNF a T TNFB
7. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 OD 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 00 20 1 10000000000000 O 0 5411 0 0 0 0 0 0 RMPH 1000000000000000000000000000000000 0 0 000000 0000000 000 0 0 PWM Borrebaeck In Vitro Inmunization in Hybridona Technology Elsevier Publisher New York 1988 HHHHHHHHHHHHHHHHHHHHHHHHH 1640 capsular 1000001000 30 0 10000 0 0 100000000 10000 20 500x 90 500 1000
8. 0 0 0 000 0090910000000 0000 EE 10000000000000000000000000 0000000 000000000000000000 ointnent H 0 O 5 000000000 0 0 100000000 Alzal 0 Phar maceuti cal 0000 0000000 0000 0 1000 0 U S Patent 4
9. 2 1 30 4 11 9 11 5 2 4 3 4 3 8 2 5 4 9 3 1 2 7 8 9 0000000000000 40 600 150 000000 PANC 10 0 4 100 5 3 7 100 10 PBS 100 00020 0 0 00 196 100 100u IODO0 0000000 30 RW 1001 90 100u 10 10002000 21 1000000 110000 77000 2 0 HHHHHHHHHHHHHRMHHHHHHHHHHHHHHHHHH RMH 0 0 WN NRW a a 0 00 00000 16400 0 capsular 1000000000000 20 0000 500x g
10. 00 30 00000000 50 60 75 85 90 95 0 96 97 98 0000000000000 00 50 0000000 000 BLAST 2 0 0000A Itschul et al 1990 J Biol 215 403 10 NCBI 0 0000000000000800 080 08000 00 00 00 0 4 00000 2 5 202 0000000000000 BLASTP PA ML00 250 BLOSUM 620 HHHHHHHHHHH HHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHH 100000 1100000000000000000000 1000 00000000000000 10000000000 10000000000 0 0 0 0 7 07 0 0000 000 0 10000000000 VH 0 0 0 0 0 0 0 0 0 0 0 00 0000000 Fab Fab 0 Fd 5 0 D scFV
11. CN 0O AO _ lt oy sc iais 2 s O A 05 _ x _ mi 5 H EJ O
12. 0 00 0000000 0 0 0 RW ATCC 0 54111000000 24 0000 100 00 24000000 0 Fap Fv 26HHHHHH 0 24 102800 29900000 24 0 31000000 38
13. 10000000000001 00000000000000000 Kay et al Nat Genet 24 257 2000 Nakai et al Blood 91 460 0 1998 0 000000000 U S Patent Nos 5 700 4700 5 731 1720 0 5 9 28 944 0000000000 U S Patent 5 501 979 0 0000000 00000000000000 000000000000000 000 000 OD U S Patent Nos 5 624 820 5 693 5080 5 6 65 577 6 013 5160 5 674 7030 0 O W PO WO92 052660 092 14829 1000000000000000000 0000 U S Patent 5 719 054 1 0000000000 cw 0 00000 U S Patent 5 561 063 0 0000000000 00 1000000000 0 0 U S Patent Nos 5 82 1 2350 5 786 340 6 110 4560 000000 0 0 0 0 0 10000000000 000000000000000000000000000 1100000000000 0 00000000000 00000000000 00000000000000000000 00 0000000000 00000000000 1000000000000000000000000000000000 1000000 0 1000000 0 00000000000000 0 1000000000000 1000000 0 000 00000 00000 0000 0000 0000 0 00000080 HHHHHHHHH
14. HHHHHHHHHHHHHHHHHHHH myeolblastic H 0 00000 1000000000 0 100 1000000000008 0000 CLL 10 0000000 HHHHHHHIH WW 100 000000 000000 0 TO 0 0 0 0 0 0 0 0 ATB 0 0 70 LGF 00000000 0 100000000 0000 0 0 0 000000000 0000000000000 00 000 000 0 HHHHHHHHHHHHHHH 0 HHHHHHHHHHHHHH JP 2006 510352 2006 3 30 28
15. 0 PTA 5412 5411 100000000000000 8 JP 2006 510352 2006 3 30
16. cDNA DNAI 50 10000 1000000 1000 1100000000000 10000000000000000000000000000 10000000000 0000000 Id Spring Harbor Laboratory Press _ 1 __ 1 gt 5 N _ _ se CEC FPE T
17. 0000000000000000000000000000000 1000000000000 00000000000 000000000000000 00000000 90 00000000 0 HHHHHHHHHHHHHHHHHHIH ENHE 0900000 00000 10000000000000000000000 10000000000000000000000 RM 0 0 000 00000000 00000000 00000 05001310 DJ 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1000000000000000000000000000000000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1000000000000000000 TAA H 0 0 0 0 0 0 0 0 00 00 0000 00 00 000 00 0 00 00 0 l 00000000000000000000000000000000 JD UH 0 0 000000 000 000000060 12 U S Patent 4 814 275 CD33 ML95
18. 50 JP 2006 510352 2006 3 30 6
19. 1000000000000000000 AgRMH 460 00000 46 000 0 000000000 00000000 0000000 488 00000 49000000 0 I 0 0 0 0 00 0000000000000000 0 10000 00000 0 0 00 0 100000000 AgRMO 0000000 0000 00000000 AgRMH 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 00 10000000000000000000000000 AgRM4 00 000000 00000000 00000000000 0 530 100000000 5400000 53 000 000 0 100000000000 540000 5 JP 2006 510352 2006 3 30
20. 53 0 0 0 0 0 0 53 53 10 0 AgRMH 00 000000000000000 00 10000000000000 10 240 0 0 400 000 U 10000000000000000000 620 20 62 0 0 0 62 JU UU UJU UD 900 LU AgRMH 0 0 0 0 0 00000000 0 0 0 0 0 0 PTA 5 000000000000000000000 00000000000000000 67100000 30 100100000000000000000 67100000 00000000 AgRMD 00 000000000000670000 10100100 0 0140 00 0 1 0000 00 00000005670 0 67 40
21. Harlow RW 45H 1000000000000000000 199 0000000 450000 000000000 00 00 0000000000000 00000060 RM4 10 4 4 8 8 2 2 BE 7 7 3 3 0 4 0 2 7 0 3 0 4 0 4 0 3 0 3 0 3 20 0 3 0 3 0 3 0 4 0 3 0 3 0 3 0 3 0 3 0 3 pega 073 30 073 0 4 1 18 0 3 0 3 0 3 0 3 E 0 3 0 3 0 3 40 RMH RMH 000000000000000000000000 amp 00000000000 RM HH pH H IHHH IHHHHHHHHHHHRMH 2 RM 1 0 125 140 17 rCi u 0 0 12x 750 000000000 0 00000000010 1000 100000 1004 990000000 10 100000000 30000 100u 990000000000000 lO 10000000 50
22. CN I E O C ma PI N gt E O lt CN 5 _ 9 s 9 L I lt gt
23. di fi cati ons Marcel Decker uu uu 0000000000 0000000000000 0 10000 0000 cDNA 60 0 70 0 08040 0000 00000 00000000 00000090 0000000000000000000000000000 100000000 0000 00 00000000 00000000000000 000 000000000 000000000000000000000000000 22
24. p53 0 3190 3600 TSP4 j f os GCN4 10000000 000 0000000000000 00 00 0 00000000000000000 000 10000000 0000 10000000 10000000 10000000 0000000 100000060 0000 10000000 0 10000000 0 000 00000 00000000000 10000000 10000000000000000000000000000 0 00 00000000000 100000 000000000000 10 20 30 50 2006 510352 2006 3 30
25. 0 0 00 00 000 0000000000 0 0 0 000 0 1000000000000000000000000 t L D 000 0010190 0000000000000000 5 00000 0 0 0 0 0 0 0 0 0 0 0 0 00 00 0 wch 0 0000 550 0 Xpress Invitrogen Carlsbad Calif USAO 0000000000 0000000000000000000000 0 HHHHHHHHHHHHHHHHHHHHHHHHHHH HRP 0 0 0 0 000000000000 00 000000000 0 0 00000000000000000 100000000000000000 0000 J 2pu 5 1220 IDHHHHHHHHH HHHHHHHHHHHHH HHHHHHHHHHHHHH 00 00000000000000000000 00000 00000000000000000000000 100000000 o o p ht dehyde Molecular 5 Eugene Al exa 350 Al exa uor 4880 Alexa Fl uor 5321 Al exa 546 Al exa 568 Alexa 594 Al exa 6470 0000000000 0 0 BODI PY 493 5030 FLO R6G 530 550 BODI
26. 31 JP 2006 510352 2006 3 30 RM2 1 1 0 6 0 2 bi 7 7 2 2 9 9 3 3 4 4 0 4 gt 0 4 10 0 3 0 4 0 3 0 3 EE 0 3 0 3 0 5 0 4 0 3 0 3 0 3 20 0 3 0 3 0 3 0 3 0 3 5 0 3 0 3 0 3 0 3 0 3 0 3 30 RMHHHHHHHHHHHHHHHHHHHHHHHHHHH CIhHHHHHHHHH IHHHHHHHHHHHHHHHH
27. 8 EIA 0 0 0 0 0000000000 361 SK BR 3 MCF 7 20 Colo205 LoVo LST174 HT 29 SK LU 1 NCI H661 1435 Calu 1 549 30 U 87 MG MC ICX Daudi 1 RE A375 MeWo 40 5 28 4 SK OV 3 1 1 A431 00000 50 34 JP 2006 510352 2006 3 30
28. N m _ 2 _ _ 27 Q 0 s TER T O _ x lt 22 L E NEE lt n o g 5 O g E l a _ T O O I et Em m lt s 1 et 0 8 O He O E o o 1 1
29. 2 2 RMHHHHHHHHHHHHHHHHHHHHHHHH 0 1000000000000 0 0 0 000 00000000 00 1000000 2000000 10 100000000 5 10 5 Kb 1000 50000000000000 0 1000 0 500 100000 120 250 250 50 0 1000000000000000000000 70000
30. 9 2006 510352 2006 3 30 gt 0 0 O PTA 5411 0 AgRMH I 0 0 0 0 AgRM AgRMH RM4 P
31. 20 2 gt PTA 5412 0 00000000100000700 1 0 F ab 2 0 0 0 0 0 0 10 30 50 3 2006 510352 2006 3 30 HHHHHHHHHHHHHHHHHHHHHHHH 19
32. 100000000000000000000000 100000000000000000000000 3000 00 5 3 0000000 0000 000 10000 00000 5 0 0 0 0 00 0 0 00 0 0 0 0 0 0 0 000 nRNAH 0 00000 10000 10000 7000000 00000000000000000000 0010 0000000 1000000 000 000 HHHHHHHHHHH 1000000 1000000 00 1000000 000000000000000000000000000000000000 0000000 1000000 1050050000000 00000 70 000000000 0 00 0 ptac ptrp lac 0 000 0000000 00000000000000 0 100000000000000000000000000 ADH 0 LEU 20 GAL 1000000000000000 0000 Ausubel et al In Curre nt Protocols Molecular Biology Vol 2 13 ed Greene Publish Assoc 6 Wiley Interscience 1988 Grant et al 1987 In Methods in Enzynol 153 5 16 544 eds amp Grossman 1987 Acad Press N Y G over Cloning 3 IRL Press Wash D C 1986 Bitter 1987 In Methods in Enzymol 152 673 684 eds Berger amp Ki mel Press Strathern et al The Molecular Biology of the Yeast Saccharonyces 1982 eds Cold Spring Harbor Press Vols 110000000 0 JP 2006 510352 2006 3 30
33. _ o 2 Ta P m lt 1 x u mi O e n co lt lt 12 2006 510352 2006 3 30
34. 7 54 520000 8 synergy HHHHHHHHHHHHHHHHHHHHHHHHH 000 0000000000000000000000000 z w HHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHH TIME control 55 T 2 amp 2 JP 2006 510352 2006 3 30 PTA 5412 10 1 100000000 500000 10
35. MP 1o 0 18 S DF 11 MCP 11 MCP 20 MCP MEP 4 2 1 309 HCC 10 HCC 20 HCC LARC M P PARCO O 60 71 80 CKB 90 110 120 C100 1L 8 GROo GROR 78 20 PBP TGO NAP 20 M PBSF SDF 1 10000000 100000000000000000 000000000 0 uu 000 0 2000000000000 0 00000000000000000 0 Al 0 0 0 6 1000000000 5 5 0 0 00 O 0 5 AZO 5 00000000 0 0000190 RM 40000000 R 5 0 00000 0000 0000 0 0 0 0 0
36. CD20 JU DU DD DDD 900 10010010 12000000000 9 0000000 gt O PTA 5411 RW ATC 0 5411 AgRMII 0 0 AgRM2 F ab gt Fv
37. ooo H _ xx i m _ oodd _ _ _ q N EAOn gt N _ _ x CN _ N N q m aen E 58758 s es x n A TAS aA pn i 9 O X ____ k Z __ Oi ES 4 25 x _ _ 4 s Ea m 2 _
38. PTA 5411 100000000 4 JP 2006 510352 2006 3 30 N D 100000010000000000 240 0 03020 100000 IHHHHHHHHHHHHHHHHHHHHHHHHH 1000000000000 100000 100000 AgRMH IHHHHHHH1HHHHHHHHHH AgRMHHHHH
39. 10 20 30 50 JP 2006 510352 2006 3 30 26 AgRM 10 100 AgRMM RM RMO AgRMH AgRMH RM U 20 30 100 10000000 50 HHHHHHHHHHHHHH HHHHHHHHHHHHHHHH 0 0 100 100110
40. A lt gt AA a 171 7 TE O _ Z m 5 m 5 E E gt O i Px I 1 5 S a O i E _ E 5 s a T Z Hyi AE EE lt _ 1 171 O a O i ss ooN Eii O 1 ma n gt 5 EN D Q s g lt T O
41. 1 0 a O i x 2 _ ss O lt S lt 5 lt 2006 510352 2006 3 30 24 AgRMH AgRMH AgRMH 10 20 1000 100 1000000 lB H 10000000 100 1000000 10000000000000000 30 100
42. Te BR gt lt O as o lt O I 5 gt lt 25 12 29 2006 510352 2006 3 30 1 1 1 1 1 1 1 CJ gt e 0 000000000000000000 00000000000 sequence
43. Genna ed Rem ngton The Science Practice of Pharmacy 20 ed Li ppi ncott Wllians amp WI 5 elivery Systens 7 Handbook of 2000 Ansel et al Pharmaceutical Dosage Forms Drug D ed Lippincott amp WIkins Publishers 1999 Ki bbe Phar maceuti cal Excipients Ameri can Pharnaceutical Associ atio 39 ed 2000 D Pharmaceutical Principles Solid Dosage Forms Techno nic Publishing Co Inc Lancaster Pa 1993 0 000000000000 00 0000000000 00 100000000 0 000 00 00000000000 0 000000 0 000 AgRM HH HHHHHHHHHHH 0000 1000000000000 98 0 00000000000 0 0 0000 AgRMO 0 0 0 0 0 0000000000 1000000000000000000000000000 HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
44. 10 20 30 50 16 2006 510352 2006 3 30 100000000000 1000000 1000000 1100000000000 1000000000000000000 0 100000000000 R Rei sfeld S Sell Eds Anti bodi es Cancer Therapy Alan Liss Inc 19 85 U S Pat Nos 5 558 852 5 624 659 10000000000000000000000 0 0 00000000 1000000000000000000000000000000000 1100000000000 1000000000000000000 0 1000000000000 000000000000000000 0000 Lane Using Anti bodies Laboratory Manual Spring Harbor Laboratory 1999 Fitzgerald al 5 117 11075 1995 Gramet al Proc Natl Acad Sci USA 89 3576 1992 1000000000000000000000 0 10000000000000000000000000000000 Appl i ed Biosystens Foster City 0 0 0 0
45. Sci USA 81 6349 1984 Eukaryotic Viral Vectors Cold Spring Harbor Labora tory uzman ed 1982 Sarver et al Cell Biol 1 486 1981 0000 HHHHHHHHHHHHHHHHHHHHHHHHHHHHH 0 100000000000000000000000000 000000 1000000000000 00 0 000 0000000 VSV 10000 Patent 5 604 090 1000000000000000 424010 000 00000000000
46. _ O i 1 2 C C C lt E Fa O CN O _ C o s 2 _ lt lt E N se lt lt wm 7 2006 510352 2006 3 30
47. 00 HHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 27 JP 2006 510352 2006 3 30
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49. 35 2006 510352 2006 3 30 100 6500 800 0 0 0 0000 0 20 1 0 0 020 0 1 1 00 0 0 SK BR 30 H 4 1090 0 0 000 nu nu 40 600 5 HHHHHHHHHHH 2000 300 n RM 1004 100000 48000000000 DO00 0000O0O0O0O0000000000000000000000000000 60000 SK BR 3 8 9 7 2 74 1
50. AgRMH 0000000000 AgRMH HH AgRMH 0 1000000 000000 0000 00000000000 100 1000000 0 0 0 0 0 0 0 0 0 0 0 10 2 p530 Tn 22 JP 2006 510352 2006 3 30
51. bi nd bi ndi ng 1000 2006 510352 2006 3 30 11
52. 18 2006 510352 2006 3 30
53. Hudson Curr Opin Biotechnol 9 395 1998 0 0 00 Fabl 0000000 0 0 0 0 0 0 Fab Fab 0000 0 110000002 Fab 210000000000000000000 00000000 0000 0 0 0 0 0 0 0 00 0000 000000000 OD 10000000000000000000000 Inbar et al 1972 Proc Natl Acad Sci USA 69 2659 Sandhu 1992 Crit Rev Biotech 12 437 0 0 0 0 000008080808080808080 080 0808080 00 080 080 00 00 000000 L Va VMHHHHHHHHHHHHHHHHHUMH 1000000000000000000000000000000000 1000 00020 0 000 0000000000000000000 12000000000000000000000000000000 scFv 00000 Whitlow et al 1991 In Methods to Methods in Enzv 10 20 30 50 2 97 U S Patent 14 JP 2006 510352 2006 3 30 4 946 778 et al 1993 Bi o Technol
54. H I E I T LU SE 51 SK EF MA GN GQ SN AE AG AZ BZ H K EE ES FI G Q G H M H HJI DIL INIS JP KR KZ LG LK LR LS LU LV M PG PH PL PT RO RU 5 SD SE SG SK SL SY 1 TM TN TR TT TZ UG WZ VG W ZA MAN 10 000000000 10 72 10000 00000 00000000 00 0000 0000 000 0000 00000 72 00000 000 000 0000000 00000000 00 0000 000000 00000 00000 O 00 48024 AAO1 AA12 BA45 CO2 DAO2 DAO5 GT 4BO64 7 CAB O G24 CEl2 DO 4BO65 AAO1X AA5 7 1 ABO2 AC14 BAO1 BAS 046 40085 2 BBO1 CO2 003 182 EFO1 3301 5 AO BAO9 BMO BA51 BA52 BA72 0 76 EA2O 026
55. RMH 0 00 10 1000000000 RM 00000000 0 HHHHHHHHHHHHHH HHHH HHH 100 250 250 500 50 100000000000000000000000000000 PCRH 00000 0 000 1000000 00000000000 000 10000 0 Wa 700000 JP 2006 510352 2006 3 30 17 10 Cloning Laboratory Manual 3 9 ed Co I n x Sarprook et al 2001
56. Esa O __ T o 5 E 2 n EN rn lt _ lt ss ma zz pa 1 lt C i lt _ O 117 lt O _ _ 1 _ __ 5 T O ps 2525 C Ho 00 O 117 0 __
57. 10 20 30 50 2006 510352 2006 3 30 23
58. 1000 100 100 AgRMH N Par si BASF Crenophor EL H 1000 5 50 100 10000 100 1000 100000 100 10000 1000000 000 AgRMH 25 2006 510352 2006 3 30
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60. PY 558 5680 BOD 558 5680 564 570 576 589 PY 581 591 PY 63 0 6500 BODI PY 650 665 BODI Cascade Cascade Yellow Dansyl Oregon Green 4880 Oregon Green 5141 Pacific ue 69 0 0 0 Texas Red 0 0 0 0 0 10000 O 0 U M RI 1 11 111 11 111 11 11 11 11100 111 IIT 11000 000 11 1 111000 111 111000 0000000 111 000000 0 0 100 1000000000000 00000000000000000000000 100000004 0000000000000000 seu 30 cr 37 Rup 997 105RhD lll nH 125 131 149 153 Sr 186 Re 188 Re 194 203 211 At 212 Big 213 Big 212 PD 223 Ra 225 Ach 227 Ach 228 Tho 0000 000000000000000000000000000 1000000000000000000 000000 0 000000 00 00000 0 0000000000 0000060
61. U S Patent 6 599 505 0 0 O0 O CAG o U S Patent 6 596 503 1 00000000000 0 0 056 0 0 IHHHHHHHHLeHHHHHHHHHHHH BR96 0 0 Rituxan 0 Herceptin Her 2 neu 0 Bevaci Avasti n n O Bexxar 0 Oncol ym Edrecol 0 3F8 H 00000 0 0 MDX 0 00 0 0 0 0 0 0 0 0 C225 0 0 0 Cet uxi mab 0 0 1gG HH ATCCH PTA 5411 1 RMHHH 52 AgRMH
62. 0 0 0 10 50 O0 PWM Borre C ed 0 0 0 000 RPM 1640000 0 0 5 1070 1000000 5 95 37 0 35 100000 1500 0 10 0 0 150 0 1 6 10 000000000000000000000 00 00000 10 0 96 1 10 0 00000 00000 1 104 MHHHHHHHH 4 40 107 1 6 102 0 10 FCS 00 2 0 16400 HATH 1 20 4 100000000000000 100000000000000 1000000000000000000000 1000 10 2650 RPM 1640 100000000000000 10000000000 10000 1000 RM EIAI 0 0 10000000000 30 JP 2006 510352 2006 3 30 U 87 MG MC IXC SK LU 1 549 Caiu 1 NCI H661 75 MeWo SK MEL 28 1 1 M SK BR 3 7 gt Sim Colo205 HT 29 LoVo Caco 2 SK CO 1 Daudi Raji 4 SK OV 3 431 I D 5 5 5 N
63. 0 50 0 0000 00 10 5 0 0 0 0 1640 000 5 1090 00000000000000 00 5 95 0 0 0 0 37 lt 000000 35 15000 0 0 3 1070 150 1 6 10 Harl ow and Lane 00 96 1000 100 1 10 0 000000 00000 1 104 MODO0000000 4x10 M 1 6x 102 10 FCS 2 nM 10000 RPM 16400 O 10 20 40 00000000000000 00000 000 00000 1000000 10 FCSH RPM 1640 1000000 H B OD EIA H 0 Harlow D 33 JP 2006 510352 2006 3 30 RM 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 000 000000000000000 American Culture Collection ATCC HHHHHHHHHHHHHHHHHHH Inmmulon 9600000000 2x 10 1000 3700000000000 Lane D Ant i bodi es Laboratory Manual Cold Spring Harbor Laboratory New York 1988 RM 0 0 0 0 0 0 0 00 00000000000000 1000030 000000000000 10 O
64. 0 AgRMI 10 AgRM AgRMH 0 0 RW AgRM uu 0 0 AgRMH 00000 AgRMH 0 0 2 10000000000000000000000000000 AgRM AgRMH A gRM2 100 000 0 AgRMH U 0 AgRM 000 10000 AgRM 40 AgRMH AgRMI 0 AoRIW 000 000 U 0000 0 0000 0000 100 0 0000000000000000000 PTA 5412 RW AT C PTA 5411 RM4 100 PTA 5412 RW ATC O PTA 5411 000 00 00 RM4 5412 08 00000000 5411
65. 0000000000 10 20 30 50 13 2006 510352 2006 3 30
66. 3 34 1 4 11 1 4 2 3 12 4 2 z 00000000000000 40 60 0 150 000000 Colo205H 4 10 0000000000000 500 30000000000 720000000 100 1001 1000200 0 10 gG 100 90 100u 30 0 100 90 100p 10 100 100000000 1000200 1401000000000 300000000 2110000000 10000 1000 10000000000000000000000 1200000 10000000000 7700000 10000000000000000000000000000000 AgRM 5 IHHHHHHHHHHAgRMHHHHHHHHHHHHHHHHHHHH Ag9RMHHHHHHHHHHHHHHHHHHHHHAgRMHHHHHHHHHHH 10 20 30 10435 3728 64244212008 36 2006 510352 2006 3 30 5538 6 7 130483 18640 3297943041 82009219519 170749 31873 1645 26212 60 62 122229 40601 97656525317 161964235267 1025 10 5 8 0 0000000 nu 100000 700000 100 9 100 10000000 2100 HHH
67. HHHHHHH Panc 1 Col o205 10 20 30 37 JP 2006 510352 2006 3 30 gt RM2 1 1000007 10000 100 194 1 1 2 3 4 5 6 7 8 9 PBS W gt T RM2 RM2 amp 38 JP 2006 510352 2006 3 30 51 Irt d 1000000000 16 28 2060 35 04 0000 1630 2006 01 0000 16 28 0000 2006 01 1630 0000 21 2006 01 1 15 2 08 20060 00 1 21 0000 3753 2006 01 21 08 33577 2006 01 33 53 9510 20060 0 33 577 0 5 00 5 00 89000 LS SD SL SZ TZ UG Ay AZ KG KZ MD RJ TJ Z ES FI
68. HHHHHHHHH 00000000 000000000 00000000 0 DOTAPH 0000 U S Patent Nos 4 844 9041 5 000 9591 4 863 7400 4 975 282 G BCO BRL Gaithersburg 00000 0 1000000000 0 1000000000000 0000 U S Patent 5 861 3970 0 21 2006 510352 2006 3 30 U S Patent 5 459 127 100000000000000000000000000000000000
69. HHHHHHHHHHHH RMHHH RMH 72100000 D RM 1 0 140 17 nCi u g9 O0 O 12x 750000000000 10000000010 1000 ag 1001 g T 10 100300 IHHHHHH100u g 10 105 10000000 0 0 0 500 800 00000000600 00000000600 20 1 0 0 020 0 1 1 00 0 0 D PANC 1H 0 4 10000 0000 40 60 0 5 D 2000 300 0 1004 100000 48000000000 000000 10000000 30000 0 50 32 JP 2006 510352 2006 3 30
70. HHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 1000 2000000000000002 000000000 10 2006 510352 2006 3 30 10000000000 V 0 00 Spatola 1983 in Chemistry of Acids Peptides Proteins Vol 7 267 357 Pepti Backbone
71. I Z s 5 _ ss __ o oad _ E O gt 20 2006 510352 2006 3 30 HSV tk Wogler et al Cell 11 223 1977 GANO H gptH 6 AAV H 0 0 D D 40 S V40 Cone et al Proc Natl Ac
72. JP 2006 510352 2006 3 30 19 JP 11 2006 510352 P2006 510352A 43 8 3 30 2006 3 30 1 C12N 15 09 2006 01 C12N 15 00 48024 A67K 39 395 2006 01 AG1K 39 395 48064 35 0 2006 01 AG61K 39 395 48065 1 35 02 2006 01 A61P 35 00 4CO85 1 3 04 2006 01 A61P 35 02 4 4 5 38 21 2004 536268 2004 536268 71 505091260 86 22 15 9 11 2003 9 11 85 17 4 27 2005 4 27 86 PCT US2003 028806 87 W02004 024874 87 16 3 25 2004 3 25 11211 31 60 410 366 74 100102978 32 14 9 11 2002 9 11 33 05 74 100128048 72
73. TA 5412 0 0000 000 000 0 0 0 0 00 0000 0 1000000 20003 0 70 00000000000000 1000 HHHHHHHHHHHHH 0000000 0 0 5 0 00000000 00000 100090 HHHHHHHHHHHHHHHHHHHHHHHHHHH 10000000000000000000000 AgRMH 0 0 RMHHHHHHHHHHHHHHHHHHHHHH RM4 199 v gt N 2 N AgRMAH 00000000000 8 2 100 0 1000 0 100000000 198 190 IgA lgM HHIgE
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