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空気清浄機の浮遊ウイルスに対する 除去性能評価試験方法

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1. 2 20m3 32 m3 3 20m 32 m3 1 JIS
2. 2mL I RR 0 015 20mL 1 5 2 10L 4
3. 120 cm 1 NE C16 23 5 C 50 10 RH 20 5 C 4096RH
4. MEM Minimum Essential Medium PBS Phosphate buffered saline MEM PBS 10 MDCK Madin Darby canine kidney MDCK Agarose 37 C 596CO 10L b TCID50 509 tissue culture infectious dose MEM Minimum Essential Medium PBS Phosphate buffered saline MEM PBS 10 50L 5 FBS f
5. 0 1 2 1 2 phosphate buffered saline PBS 10 10 PFU mL PFU Plaque forming unit 10 gt 10 TCIDso mL TCID50 50 tissue culture infectious dose 3
6. 6 MEM Minimum Essential Medium PBS Phosphate buffered saline MEM PBS 10 35 1C10 20 45C NB 35 1 24 10L a
7. Code 05514 NA Difco 0 596NaCI 1 NB Becton Dickinson 233000 0 596NaCl 35 1C 18 2 35 1 C 100rpm 5 1 NA 0 59 6NaCl 60mL 5mL 10 CFU mL CFU Colony forming unit 5mL 10 PFU mL PFU Plaque forming unit 1000 1 35 1 C10 20 45 50 NB NB 0 59 2NaC 0 59 10mL 35 1 C 18 2
8. 1 2 Escherichia co7 phage Phi X174 ATCC 13706 B1 NBRC 103405 Escherichia coli ATCC 13706 NBRC 13898 Escherichia co 7 phage MS2 NBRC 102619 Escherichia coli NBRC 3012 13965 106373 A Influenza A virus H1N1 APR 8 34 ATCC VR 95 Influenza A virus H3N2 A Aichi 2 68 ATCC VR 547 Madin Darby canine kidney MDCK 20m3 32 m3
9. 2 3 4 1m
10. 2011 23 7 4 7EMA 1 X 2 3 4 5 1 2 WHO BS L 2 2 Biosafety Level 2
11. 0O 1 70 cm 180 cm 1 2 3 20m3 32 m Yamazen YBR C 25 BS B 25 on
12. 5 1 JEM1467 2 1 1 h 1 25mg m 30 0 15 mg m EN ON 1 99 30 90 3 APIC guideline for selection and use disinfectant 10
13. MS2 Phi X174 MS2 Phi X174 JIS 20 40nm 20 100nm 9
14. JIS K3800 1 40L 2 80L 5 1 3 2 0 90 4
15. t y aix b y azx b yiLog PFU 10L air x t Ay Ay t az ai 1 909 2 999 1 1 x100 96 7 2 0 PFU 10L air Log e y 0 0074x 4 55 R 0 64 y 0 0938x 4 91 R 0 99 10 20 30 40 50 60 1 1
16. seward BA6141 standard bags 260RPM 2 35 1 C 1 50mL 3500rpm 10 50mL 2 0 22xm Stericup MILLIPORE SCGPU02RE 2 Phi X174 10 10 PFU mL MS2 10 10 PFU mL 65 69 x10 Nm Phi X174 10 10 PFU mL MS2 10 lt 10 PFU mlL 10 100 X174 3 MS2 6
17. etal bovine serum DMEM Dulpecco s modified Eagle s Medium MDCK 50L 96 CPE cytopathogenic effect Reed Muench 10L 6 4 PFU mL 1 1 1

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