ZEN2012 Lambda Mode 簡易マニュアル
Contents
1.2. Hil JGR Fl bi MET PEt ll J EF tg EE RY RR oR ER SE I JT ol Ll i LE a 400 496 nm 729nm 225nm Use Dye Color Detector Range Resolution lm Ch1 415 735 nm LSM710 9 7 NM LSM780 8 7nm ChS 496 729nm 9 7 nm Ch2 415 735nm Passes 1 Reflection Carl Zeiss LSIMI 4 ZEN 1 2 amp Linear Unmix 2D
3. Intensity Mean Sum Automatic Extract CFPPPO5 iSRgRSNO VY Lambda coded None Auto scale Weighted unmixing Show channels with statstical confidence vV Show channel with residuals Save to spectra database Autofind ACE Linear Unmixing Apply to hardware 3 3 Apply to hardware Channel mode 3 2 Extract Apply to hardware Channel Mode
4. ACE Autnmatir CompnrEnt Extrarhinn Numher rnrmpnnents 7 C Carrr ACE 1 Carl Zeiss LSM710 780 ZEN 3 2 Extract 4 Lambda Mode Unmixing Extract Bands
5. 6 Visible Light Invisible Light 405nm NLO 2 Visible Light 488nm 29 Carl Zeiss LSIMI 4 ZEN MBS MBS Visible Invisible 2 MS 58 MES 458514 MS 458 1 MS 5R 1 5 MES 488 4 MES 488551 E33 ES TR Plate LU i BS 40 TG None F MES TBHRE2D GFP 488nm FF Non MBS 488
6. ave tra datahase New Folder OK Region No Name Comment Comment save Saw tn Spectra Datahase RiEEIrm 1 REEIr RiEEIT 3 rm RiGH MBS A458 InVis Plate Plan Apochromat 3w1 40 Oil C New Folder EAMEe File Spectra Database Carl Zeiss LSM710 780 ZEN 2
7. Unmixing Apply Unmixing Carl Zeiss LSIMI 4 ZEN 2 Online Fingerprinting Online Emission Fingerprinting Linear Unmixing 4 3D A Time A 3D Time 2 1 Light Path Online Fingerprinting EE Light Path Light Path Laser nlinE F
8. Text a rds corlenl Spline Normalize AutnmaTir colored Save to spectra database _ Auto find fT ACE 15 Linear Unmixing Unmixing Carl Zeiss LSIMI 4 ZEN 1 5 Linear Unmixing 1 5 1 Case 1
9. LSM 0 ZEN2012 Emission Fingerprinting 2 Linear lnmiwimn 2013 10 Carl Zenss Mncroscopy Co Ltd Carl Zeiss LSM710 780 ZEN ZEN2012 ZEN Lambda mode Lambda mode 1 Lambda mode rs 4 1 1 5
10. 4000 3000 2000 1000 500 550 Linear Unrmixing Linear Unmixing Auto Scale Weighted unmixind Unmixing Show channels with statistical confidence Show channel with residuals Unmixing Overtsy ITTTRN R Carl Zeiss LSM710 780 ZEN 1 5 2 Case 2 1
11. Gallery 4 imensions Gallery rag i i Y Interpolation ingle Channe Quick Color Setup Positions Stage Dimensions Palette Range indicator Carl Zeiss LSM710 78O ZEN 1 3 amp Snap Z Time Series Start Experiment 2D X Y 3D XYZ Time Series XY T 4D XYZ T
12. Linear Unmixing Processing Linear Unmixing Select Components RERETCGTTRRIE35m 1 Correlation Sritrh F PALM Unmixing hlEthnd PararrWEtEr5 Fawonie ahH1H karmbds FRET EYFP Multi Channel Umeoine utoascaE nHiErwre iritErwals Cisplay hannel wrth residuals ome RE paramieters fro mags Auto scale Display Channel with Residuals Background
13. AA Time Series Lui PC Online Fingerprinting Carl Zeiss LSM710 780 ZEN 1 4 Unmixing Unmixing Unmixing Delete Dye Color YTable Region 1 WLlLambda coded Diagrarm Region 2 Region 3 None Auto scale Weighted unmixing Show channels with statistical confidence wy Show channel with residuals Save to spectra database Auto find A CE Linear Unmixing
14. Linear Unmixing BE F114 51 594 Online Fingerprinting Unmixing Lambda mode Carl Zeiss 3 3 1 ACE Automatic Component Extraction Lambda Mode Unmixing Unmixing Dye Region 1 Region 2 Auto find ACE LSM710 780 ZEN Unmixing Delete M y Table Diagram w Lambda coded None Auto scale Weighted unmixing Show channels with statistical confidence y Show channel with residuals EN OK Save to spectra ET Unmixing ACE Unmixing
15. Linear Unmixing Linear Unmixing 2 Lambda Mode 4 Linear Unmixing Online Fingerprinting Online Linear Unmixing Dnline Firgerprinting Cnlnr Detector Range Resolution Lse Dye Color Detector Range Resolution Ch1 1 78RTirm ww GFF Rs1 5 B5rrm 7 nm Chs 7 nm ww TFP RSg TIrm Ch2 15 3 mrm Passes 1 Reflection Passes 1 Reflectinn 6 MES 488 Visible light MBS 4B8 Visible light 2 DE a Invisible light Plate 5 Invisihle light NaneLsM NoneLSM P 4 EE Focus Lt FnCLl5 T PMT I T PMT Carl Zeiss LSM710 78O ZEN 1 Lambda Mode
16. SS ER NS IDB RIRSGRSB A 1 3 nnn 8 OE RON 9 1 5 Linear Unmixing rn 10 1 5 1 mn 10 1 5 2 er 11 Online Fingerprinting ER 13 2 1 mm 13 2 2 14 SE RR 15 3 1 ACE Automatic Component Extraction 15 2 16 3 3 Apply to hardware 16 Carl Zeiss LSIMI 4 ZEN Lambda Mode LSM710 780 4A 4 Emission Fingerprinting
17. AB Unmix Carl Zeiss LSIMI 4 ZEN 1 1 Liqht Fath howall 0 Light Path Lambda Mode o Lambda Mode nhne Fingerprinting 15 5 rm BB nm mm Light Path 415 735 nm LEHIL Il Laser MBS GFP Laser Light Path Lasers
18. A Linear Unmixing Emission Fingerprinting 1 1 ul 1 2 Linear Unmixing 1 3 XY Z Time Series 1 4 1
19. 5 Linear Unmixing Unmix Carl Zeiss LSM710 7 BO ZEN 2 Case 1 Case2 Case 1 3 Case 2 lt Unmix A B A A 1 B B AB AB A B A B
20. irgerprinting MBS nn rm re 5E LE lar Detector Range Resolution i Po asFar Dye NonetsM Inyisihle li FavOrie 2010 lambda Light Path uH Carl Zeiss LSM710 780 ZEN 2 2 Gain Master Digital Offset Digital Gain Gain Master _Tracks Channels Digital Gain Digital Offset ambda RS Bs
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