分光測定の基礎
Contents
1. 1 8 3 GRE Ph FEN N wi LC SHORE MN M N N2. 4 25 N No 7 T 1 N N 4 26 4 4 log log 1 4 27 A log 1 N M 4 28 A In N M In 10 4 29 exp A In10 1 N No 4 30 N N 1 exp 2 303 A 4 31 N N 4 N No 2 303 A 4 32 Ae A 2 303 4 4 33
3. 2009 vol 67 i FE 4 1 27 2 11 507C 55
4. x BHT LY FORMATS SOE COTTA N 445 677 681 100 j ia K 80 e a 3H H 60 f X f i iol il ah j 2 7 ji 20 a 620 640 660 680 700 720 nm 4 1 29
5. 190 370 nm 486 5 nm Z b HRD D V 1 D P HS 370 900 nm H 486 5 IIITLUM i ed td GS OA G B 200 250 300 350 400 450 500 nm 4 1 2
6. A 2009 vol67 BA 2 3 680 nm Va LED
7. 4 1 23 4 1 14 1 f 4 D H 77 K
8. THRI 0 1 f 0 2 4 2009 vol 67 2 0 1 5 10 08 07 06 05 0 4 0 3 0 2 0 1 0 05 0 00 A 2 4 1 17 2
9. 4 1 26 4 1 27 4 1 28
10. 12 nm eax 5nm OC lt
11. FRR Z A L 7 443 NN 4 1 28 1 1 8 1 8 1 T
12. 1 d a b 4 1 8 a b 2009 vol 67 CCD 1 d Ah ee 4 1 9
13. 4 1 3 AE A AT CT CORD 1 D 3 100 lt D CO Aa NO N CO O bho 10 200 300 400 500 600 700 800 900 1000 11001200 nm 4 1 3
14. 1 f 3 XE WE 80 HR lo FATEH Io Ipt fla 0 7 1 ok ie a 4 ERODE ar ji TE Io Ip Ip 7k 4 1 20 Ye ees FES WE 7
15. 4 2 wk HOWE lt 1 000 nm l a 1 Lambert Beer c c mM 7 7 2 d 7 77 Cs dal k Tdl 4 1 1
16. a BC P B AO OB A AO dsina OB dsin 4 11 sin a sin B na 4 12 A AER n 6 0 HL n WIS 0 z 0 RRA FUE RD SRE B
17. 1 13 1987 P 74 2 3 4 1983 P 11 4 MPS 2000 5 SPD 10A 6 4 1983 P 13 7 K Shibata Methods of Biochemical Analysis ed D Glick Interscience Publ Vol 7 1959 P 77 8 4 1983 P 22 9 Butler W L and Hopkins D W Biochim Biophys Acta 12 1970 439 10
18. MK DS 273K OC 77 K IONE im 7m 4 1 24 23 C 600 650 100 600 650 100 nm 4 1 25 9 4 1 26 1 79 C
19. 2009 vol 67 650 nm 700 nm T 700 nm RERE
20. 1 IOR 4 No 4 P P Not 4 23 action spectrum KO 444 Assy
21. pitas 4 1 8 bD a JB a 9 4 sin g nsin 8 4 13 sin w nsin f 4 14 ata A 4 15 4 16 COC 2 p
22. Ip Ja se Bg PM 4 1 21 500 600 700 800 nm 4 1 22 7 0 200 300 400 44 PAE VIC SEC
23. 1 8 2 10 nm
24. AG HPLC l b l b Torr 170 400 nm
25. 1 C 1 CHS ERR A aS I Ri a ERIK lt Eo A SAS gt gt 4 1 5 433 Sat S Wit SiG 4 1 6 1 C 1 4 1 6
26. 4 1983 P 99 11 MPS 2000 12 Joliot P Methods Enzymol ed A San Pietro Aca demic Press Vol 24 P 123 13 Ogawa T and Inoue Y Biochim Biophys Acta 724 1983 P 490 447
27. 684 nm IT B N N A 1 A 630 670 nm
28. 100 T 4 1 17 8R Us amp eas T LL BOE ORI A logT log l h DS c T log7 Ac c AT AS ee Se At vA 4 17 g gs c Ac AT AT c Tcdd 7 In7 4 18 Ac c 1 _ AT T T z 4 19 439 al A A ho 0 0 2 0 4 0 6 0 8 1 0 FAET 4 1 18 CO F A7 1 7 4 1 18 d TinT _ oe IT l taint 1 0 4 20 InT 1 In 1 T e T 0
29. HS 440 A B C 200 250 650 700 750 nm BY 4 1 19 6 4 1 19 A B C 750 nm D
30. 2009 vol67 4 1 22 A B C
31. T 2 620 nm 1 II CO HCO K 4 1 30 10 15 nm 72 RARE RUE 600 700 nm
32. OWA LE eS Pi W 7 HS P 6 N t 4 24 6 quan tum yield Pi Not 6 N t Not 6 N No 4 25 RIX N N BIS N M 1
33. 2 a 2 15674720 Le ncn li 2 IC 4 1 10 1 4 10 4 S 10 FOR 4 1 11
34. PM b lt Als c a J 4 1 23 Riam aak 8 442 mL eo ig d 1 TREDE dD ZD 4 1 24 4 1 24 T 4 1 25 680 nm 77K 120 a
35. extinction coefficient c MM gg mM milimolar extinction coefficient 7 7 transmittance T percent transmittance T log 7 absorbance 4 4 log log sc 4 10 0 Lambert Beer a b c KW 1 a 2 3
36. 434 G M S b pE S G M S c Ae ri d Oc p LM 4 1 7 1 4 1 7 5 2 3 n 1 n 2 3 4 2 2 b 1 c 2
37. h 7 Z 7 3 1800 600 A 400 200 198 60 40 A A ae A Ul fe Ea w EON eau NS ti 10 ACL L 550S N ko Seme e 6 m E a 4 EBen oeann m m e D 4i aly Ep Hi E pm f as a y 0 6 T aw we A CE FT 7 aa CTC ea Ea BE meee Pill aT 100 1 1 E a E E A z eTA E 1 1 Z Z h i g
38. 700nm a ARANYE 700nm b 4 1 15 CPU eee Tn g 4 1 16 5 4 1 16 IRS 6 7 lk BAD SAK COMeSVA 512 1 f GAA ako 2 ORRE ZANT
39. TRAE D 22 ak IN E 4 1 9 435 2 Bl 4 1 10 a b 5 inn pp AAAA i _ T TEJA DA D Bo b HEF 4 1 11 He E 4 1 12 1 d 2 PbS PbS 900 nm A SG 900 nm 4 1 12
40. HPLC VOC 200 700 nm 1 e 3 T Hh BURKE 7 7 7 Z
41. RI 681 nm 700 nm 2 720 nm IL 446 Oo NO Rate of Post illumination C04 Burst CO t 10 A Fractional Absorption Rate of 02 Evolution 021 D NO B Absorbance 0 3 0 06 22 5 0 1 5 oS ar 0 0 550 600 650 700 Wavelength nm 4 1 30 13 677 nm 700 nm 650 nm IL 1 II
42. XENES 2 432 900 nm 1 000 nm PbS
43. HPLC T 438 NNMs Y Mio AAZ yF 200nm
44. 2009 vol 67 100 90 80 70 60 50 40 30 20 10 200 300 400 500 600 700 800 900 1000 11001200 nm 4 1 4 4 1 4 400 750 nm CHA RREO HAT BAZAN 10 4 1 5
45. 12 7 COR M 7 M 7 A P a Nt a 4 1 29 1 90 Hz CHS 1 AIDE 650 nm
46. 2009 vol 67 4 kdl 4 2 7 0 a ee i ee r a 4 3 n J Inh k 4 4 ae 4 5 ae 4 log I k l 4 6 Rh b COC Id I 4 1 1 431 log kn 4 7 n c log j kc 4 8 log 7 7 log 7 log ecl 4 9 s c
47. 7 lx A log log log 4 22 7 027 lt 1 Oke 7 fF 1 D 2 4 1 21
48. A Jax f A pee NL N j eo ber TT DY if Tp TH AN m _ F 8stk TH a a ee M o ee ey A gt a ee 1 _ LT THNHD i RIN 100 200 300 400 500 600 7008 8 8 S 8 3 8 nm 4 1 12 2 ee is a O H Eer i 4 4 is a a o io 4 1 13 a b c d 4 1 13 4 1 13 3 2009 vol 67 437
49. p Cy I 3 Har 1 Ay A
50. 4 1 14 MPS 2000 4 g 1 6 1 MPS 2000 4 1 14 D WI TOR G CHE Hah 4 1 15
51. PbS PbS 2 500 nm 1 d 3 CCD CCD Charge Coupled Device DKT CCD COR 436 4 AN 3 CCD OC
52. w HOKKAIDO UNIVERSITY a w Doc URL http hdl handle ng 2115 39177 me Additional Information Information l Instructions for use Hokkaido University Collection of Scholarly and Academic Papers HUSCAP 4 DNR EDERE PACH CODE Principles of spectrophotometry and measurement of action spectra and quantum yield Hiroyuki Koike Principles of spectrophotometry are described Spectrophotometer is basically composed of three parts light source a monochrometer and a detector Properties of the three parts are described in detail Techniques to measure spectra of turbid samples and the samples at 77K are also described Principles and techniques to determine action spectra and quantum yields are described l a
53. 368 4 21 DED 36 8 T A 0 434 10 80 7 A 0 1 1 0 1 f g
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