“WAKO BIO WINDOW” No.67(2005.4)
Contents
1. 0 6 2 00000000000000 DEBTDE Lipid E Lipid 0000000000000000000000000 1970 00 Westphal 0100000000000 T Shiba 0 1980 000000000000000000000000 4000000000000000 000000 Lipid Compound 506 1 15 1 O O Lipid Compound 406 LA 14 PP Precursor 00000000L Lipid coli Lipid IVa 1 Tetrahedron 26 907 1985 2 Bull Chem Soc 601 2205 1987 000 0000000 0 buumi 30 00471 24005 5 LipidA E col 2000 33700481 24006 5 LipdlVa _ 4 2000 G T Wako Bio Window APR 2005 No 67 000 00 0 6 201 000000000000000000000000000000000000000000002. 0000000000000000000000000000000 lt 00 000000000000000000000000000000000000000000002100 1000000000000 200000000000000 30000 000000 0400 Protein Digestion Enzyme 1m x 1 ac 40p 1 1 gt Substrate Solution 44m x 1 100x POD Conjugated Antibody 44u 1 1 00000 00000000 00000 4000 0000000000000 4 00000000000 00000000000 0000 200 2007 00000 2001 0 00 00000000 60 000 208 60201 ApoptosisinsimDetectionKibwako 10000000 291 53204 241000 m 297 55901 AnnexinV FluoresceinStiningKit 500 122 03991 Oo uU 120 0441 Lemso A O O 16 19821 IXPBS Powkr 000202 138 12701 Methyl Green Solution 21 W 36 000 58 000 40 000 0000000000000000 0000 0 0000 00000 0 00000 00
3. 0 BioChanj wako siyaku info article Wako Window E mail biouin owako chem cojp X 06 6201 5964 Wako Bio Window APR 2005 No 67 Adult Normal Tissue Protein 00000001000 0 INI 0000 Dpenc Bladder Blood Vessel Arte Blood Vessel Vein Brain Brain Amygdala Brain Cerebellar Peduncles Brain Cerebellum Left Brain Cerebellum Right Brain Cerebral Cortex Brain Cerebral Meninges Brain Corpus Callosum Brain Diencephalon Brain Frontal Lobe Brain Hippo Brain Insula Brain Medulla Oblongatz Brain Occipital Lobe Brain Olfa Region Brain Optic Nerve Brain Parietal Lobe Brain Pituita Brain Pons Brain Postcentral G Brain Precentral G Brain Te Lobe Brain Thalamus Brain Tonsilla Cerebelli Brain Tuberculum Cinereum Brain Vermis Cerebelli Breast Cecum Colon Colon Ascendi Colon Descendi Colon Sigmoid Colon Transverse Diap Ductus Deferens Duodenum E pidid Esophagus Fallopian Tube Gallbladder Heart Heart Aorta Valve Heart Atrium Left Heart Atrium Right Heart Auricula Left Heart Auricula Rig Heart Interventricul
4. 000 00000 Nudea 00000 Membrane 0 000000 T 00000000 cytskeltonT 5000 0 00000000000000000000000000000000000000 00000000000000000000000000000000000 9 0 0000000000 0000000000000000000000000000000000000000000 Attoglow Westem Blot Analysis 0 0 0 0 0 0 0 0 171120 10 400000000000000000000 000000000000000000000000000000000 0000 5 9 mri 0 0 1mg mi 2 1mg my 7 0000000000000 10000000000 00000000000000000000000 0000000 50 000000000000 SDS PAGE 00 0 1 0000000000000000000000 0000000000000000000 004 000000000 0000000 000000000 0000000000 Histone 0 0 0 0000000 00000 000000000 0 Vimeting 000000000 000000000000000000000000 46 GAPDH 0000000000000000000 TE 48 o Ul 4 00000000000 Tota Protein Extraction KE 0000000 00000000000 CNMCS Compartmental Protein Extraction LT gt 0000 Lane 10 0000000 000000000 d Vimentin Lae3 00000 0 0 Laned 0000000 LaneS DDDDD BU U 0
5. 0000000000000 9 Hexanoyl lysine N HELT 19991 0 0 0 0 0 0 00 0 0 0 0 0 0 0 0000 00000000000000000000000900000000000000000000000000 0 000000000 000000000000 4HNE 4 2 malondialdehide T 0000000 00000 0 601 0000000000000000 Du 00 21 7 0 200 450nm 0000000000000000000000000000000 H N HEL d uU au 1 10 100 1000 HELD 0 0 0 nmol 0000000000 00 0000000000 umggdasggugamgudguugurgmexuimnutmulul e eceeeeeeeeMeee 11 1 bu H B Bp pug p B ems 11
6. No 0000000 575 85641 000001000 40 000 80 000 80 000 40 000 80 000 80 000 40 000 80 000 80 000 40 000 40 000 40 000 40 000 80 000 80 000 40 000 40 000 80 000 40 000 40 000 40 000 80 000 40 000 120 000 40 000 40 000 40 000 40 000 40 000 120 000 120 000 120 000 40 000 40 000 40 000 40 000 40 000 40 000 40 000 40 000 80 000 40 000 80 000 40 000 120 000 80 000 40 000 120 000 80 000 80 000 80 000 80 000 80 000 120 000 80 000 80 000 120 000 120 000 40 000 40 000 40 000 40 000 40 000 40 000 40 000 40 000 40 000 40 000 40 000 40 000 40 000 80 000 120 000 40 000 40 000 120 000 80 000 80 000 80 000 40 000 40 000 40 000 80 000 120 000 40 000 40 000 40 000 40 000 40 000 80 000 40 000 40 000 40 000 40 000 40 000 40 000 40 000 80 000 80 000 40 000 40 000 40 000 40 000 120 000 40 000 40 000 40 000 40 000 40 000 I T 000 7099 000000000 0 0000000 Gene Swatter siRNA 00000000000000000 7910000000000 ORTOS Gene Swatter siRNA 00 0 sRNAj 000 0 00 7 D 00000000000 no n 0000000000000000000 2000 70000000000000000 10000000000 000000 0000000000 00000000000 0000000000000000 000 7 000000000000000000000000000000 000000000000
7. 2005 67 z Bio Window http www wako chem co jp CONTENTS UBL 0000000 0002000000 p 2 0 0 3 o 99 p 4 000000000000 p 3 00000 0000000000000 eee 5 COO OU ETHER ELETE p 10 00000000000000 8 00000000000 Gene 5 12 HOHO 00000000 00000 00 15 ISOIL Large for Beads p 13 Apoptosis in situ Detection Kit n8 p 14 000 Cayman 6 0000000 2005 000000 16 Cayman 0000000 8 p 6 R amp D0 00000000000 eeeeeeeeeeeeeeeeeereeeeeee p 16 p 7 0000 eH HR p 16 Lipid A E col if Lipid IVa p 9 000000000 O 00000 pig ad 00000 0 00000000000000000 0000000 22000000 210 SDS PAGE O UU 000000 12510 5 20 0 2029 0 0 0 000 0 0 00 0 0 000 0 0 00 0 000 0 0000002000000000 0000 210000000000000000000000000 00000000000 uuu uuu 00000 0 pH4 7 7em 00000 1300000 over night 00000 pH3510 200 11 505 SuperSep 1251 2D 200 3500V liner graciient 90 No 190 13301 3500V constant 90 120 5 20 2D 21 SDS PAGE 20mA D D
8. Array Appendix Hybridization Index and Price List DNA and RNA Extraction 112 2 gt 00000000 Wake R amp D Systems 0000 amp 00000000 RD 18 R amp D Systems 2005 Catalog T Cell mediated Cytotoxicity 0000000060000 R amp DSytemi 1000000000 000007 00000000000000000000000 00000000000000000 EET E Wako Bio Window E mail 00000000000 X 06 6201 5964 i U TN LLLI T 4 81 9 LH UO LU BLU 16 5 121 14 AJTE 5 130 15 REI LAS TRA 5 18 20 5 18 20 2 1 5 19 20 BI BEREIT HEU EAR 5 24 26 RR E ERR 8 E ARD E E d S B UE c T 5540 8605 000000000001020 0616203 1788 0000 1 103 0023 130 2 0303270 8243 00100 20920622 1005 e0000 0 082 285 6388 00 2052 772 0788 045 476 2060
9. 000 000000000000000000000 00000000000000000000000 90000000 000m0 000000000 00000 9000024 00000000000 000 8 00000 4 60min 000000000000 0000 2 1 05 025 0 1254 g well 0 0 0 D 000000002010525 1 254 0000 00000 100mA 451 000 000000 D 00000000 369 06061 00000000 1 900 366 07671 5 1200 36606071 _ 5 _ 000000 W20x 20100 01 36000 365 00681 0 00000 33000 10 01 2400 000000000000000000000000 Hindll 0 DNA 0000000000000 000000 0 0000 000000000000000000000000000 0000000000000 000000 DO OBOUO ei llillulluL lu E 000000 0 999mm 0000000000000000 661000000 QN 000 0000000 D 00000000 361 08461 __ 60200 2 20100 10 1600 364 08571 60120 00000 000000000000 2 300 10 29000 361 00541 6008 BNXF3RJ 00 000 0000 00000 4 0 0 0 2700 36203631 60200 000000 8000000000000 2 201100 00 160
10. 005 2 Ae 12 1 33 55 0 0000000000 2500 1 1 12m x 1 0 0 05 2 25m x 2 0 esee mee 1 2 6 8 000000000 50017 2 6 7 7 227 697 207 624 nmol 1 ________________________ _________________ 2 uuum 11 Yoji Kato Yoko Mori J Biolo Chem 274 20406 20414 1999 211 000000000000 2002 000 0000000 1 O 10000000 300 15581 700 0000000000 0 0 ESADO 60 000 000000 80 000 10 8 amp OHdG Check Wako Bio Window APR 2005 No 67 307 07921 00000000000 ELDO 0000000000000000000000000 0000000000000000000000 000 1 Lys0 0 0 0000000000000000 0 0 1 v Lr o HN 20000000000 ZHPODE 00000000 00 00 RE wd m 0 limpet hemocyanin i in m eU Baid 0 00 1960 d
11. 2029 858 2278 802272223072 01171 0289 O 0120 052 099 0120 052 806 biowin wako chem co jp http www wako chem co jp 05 216 4 o1 DN
12. 0 1 175 amp wKL 2 2 e0 O 0O 0 0 0 e0 0 0000 000000000 DIO 0000000000000 00000000000000 00000 0000 59 lt Lysis Solution 9 5m lt Lysis Solution 205 0 5m 000000 1011 4 00 910 3 lt Purification Solution 4m lt 00000 ew 000000 4 900 150 lt Precipitation Solution 8m 8Kx g 60 lt Wash Solution 5m g 4 10 10000 Sm lt Ethachinmate 10 D BILL g 4G 101 lt 05 000 0 00000000 4737 316 06331 ISOIL Large for Beads 20 000 0000 316 06211 15011 5010 2800 319 06201 1501 for Beads Beating 500 35000 15010 000000000 0 00 0 0 0 0 1790 0000000000000000000000 0 190 0000000000000 Wako BioWindow 2005 0 67 13 Apoptosis situ Detection Kit
13. 0 000000000 0000 75715 89 8 00098 0000 2 5 0000 5687 0 0000100000 0 00098 000 10000000 Ag Leukotriene 4 methyl ester 556 68231 Leukotriene B4 Leukotriene C4 Leukotriene D4 Leukotriene E4 Leukotriene 00000000000000000000000 00000 0 http wwwcaymanchem con Wako Bio Window gt 2005 No 67 L 83851 42 7 4 0000 71160 24 2 73836 78 9 0001060 11 900 22 600 42 000 166 600 16 200 28 900 48 200 255 000 14 800 28 100 51 900 207 100 14 800 28 100 ugudguuududuauudululiuliuuuutul tt t ACEA 00 000000000000 5 5 0 UD 0 0000000000
14. 000 80 000 80 000 40 000 80 000 80 000 574 85591 P3234035 __40 000__ 80 000 80 000 40 000 40 000 40 000 i n e B 578 85631 i E P3234082 BBBB P3234091 B i BB 8 x 8 80 000 RR E E P3234139 57685791 P3234142 40000 579 85801 P3234149 40 000 P3234150 40 000 P3234151 40 000 576 85811 3234152 40 000 P3234155 40 000 P3234156 40 000 P3234157 40 000 P3234160 40 000 P3234161 80 000 3234165 120000 P3234183 40 000 573 85821 P3234188 40 000 P3234189 120000 P3234190 80 000 P3234194 80 000 570 85831 P3234148 80 000 577 85841 P3234200 40000 574 85851 P3234201 40000 3234206 40 000 P3234212 80 000 P3234214 120000 571 85861 P3234171 40000 578 85871 P3234218 40 000 575 85881 3234226 40000 P3234227 40 000 P3234230 40 000 P3234234 80 000 P3234246 40 000 P3234248 40 000 3294250 40000 P3234251 40 000 P3234252 40 000 P3234253 40 000 572 85891 P3234260 40000 P3234261 80 000 3234263 80 000 P3234264 40 000 5 5 85901 P3234265 40000 P3234267 40 000 3234268 40000 P3234273 120000 324274 40000 ___ 234275 40000 3234276 40 000 P3234278 40 000 P3234283 40 000 000000000000000000 0000000000000 0000000 Cytoplasmic Protein 0 5 mg
15. D 197 13291 SYPRO Ruby Silver Stain reliver 5p g SYPRO 0000000 5 20 20 0000000 12 5 2D 11 0000000000060 00 00000000 190 13301 SuperSep 125 2 14000 80000 30 3007 10 1800 197 13291 SuperSep 5 20 2D 00 200000 50 750 10 1800 Wako Bio Window APR 2005 No 67 00000000 Pr 000 0 0 0 00 L AE OBE BG AHAH 0 0 0000 0 0 0 6 0 0 0 0000 0000000000000000000000000000000000000000000000000 0000000 00000000000000000000000000000000000 pl 4 kDa Sampe AQ SampleB SampleB SampleA sample Specific spots EWOO OOOO OOOD L 000000000000000000000000000000000000000000000000 0000000000000 Mida 1 1l MASCOTE E ERE MMSR AT m0000 0000000000000000000000000000000000 0 00000000000000000000000 0000000000000000000000000000000000000000000000000 000000000000000000000000 00000 u 00000000 00000000000000000000 EJ 00000000000 00000000000 EJ 0 0 0 0000000000000 Wako Bio Window 2005 No 67
16. bg a 309 15333 MOG 100P 10010 100 000 30 MHN OXP c CT 21 2019 306 15343 100 10019 70000 Wako Bio Window 2005 No 67 2 Cayman TXB 2 EASAG 0O 0 0 00 00 BO 0 0 0 00 EIA Kit Express 2 0 0 00 0000 00000 000 00000 EIA Kit Express EIA Kit 0000 uuu 18 2 0 50 p9 m 50 176 pg m 50 7 8 1000 m 15 6 2000 m Mouse Anti rabbit IgG Goat Anti mouse IgG gt Antiserum TXB2 Monoclonal Antibody BDDEAKr Express EIA Kit 11 Hamberg M etal Proc Natl Acad Sci USA 72 2994 2998 1975 5 Patrono C etal Adv Prostaglandin Thromboxane Leukot Res 11 493 2 Ellis E F Science 193 1135 1137 1976 498 1983 3j Salzman P M etal J Pharmacol Exp Ther 215 240 247 1980 6 d m B et dal Prostaglandins Annu Rev Biochem 44 669 695 41 Patrono C J Clin Invest 77 590 594 1986 000 0000000
17. 0 36703700 60200 000000 8000000000000 2 100 W 18000 369 00461 608 ___000000 8000000000000 2 0 10 2900 369 0581 00 000 8000000000000 30 27000 363 0801 000000 1000000000000 2 201100 10 16000 360 06751 GEX BNBCH 000000 4000000000000 W 2700 369 00601 6000 746 00 00000000000000000000 O43m20 20ci 00 10 16000 363 00621 6408 7063 000000000000000000000 10 27000 0 02 2400 0 000000000000000000000 Wako BioWindow 2005 No 67 0000000 20050 00000 S O e 000 200000000 0000000000000 0000000 eia 0000000000000058 0000000 MM TIME Buffer PEN DNA and RNA Synthesis 5 Competent E coli Ligation Convenience i Em Fuse Blunting Convenience Kit Bac n Roll Beads DC Vector Kit 0 0 T Gene Transfer Molecular Biology Grade Reagents DNA EE 000000000000000000 nodi S EQ and Dye eEwoger 000000000000000000
18. 0 000 HOO 12 Wako Bio Window APR 2005 No 67 59100 DNAD O OOL ISOIL Large for Beads ISOIL Large for Beads 591 0 000000 000600 0 00000000000 0591 00000 0000 0 0 0 0 0 0 0 0 59 000910 000 0 00000 06 0000000000000 DNAD 00 0 1590 00000000000 0 0 000000000000 000000 Beads 8 gt Lysis Solution 60m x 1 gt Lysis Solution 205 3m x 1 Purification Solution 24m x Y Precipitation Solution 48m x 1 gt Wash Solution 30m x 1 gt Ethachinmate 60u 1 1 PTE pem 6mx 1 ecc pc 1 UBI 000000000 5990000000000 0000000 DNA 1 200 1 2 1 OneSTEP Marker 1 Hindili digest J Lane2 1 5 DNAD 52 064 9 59 soil I A2ey
19. 000 0000000 00000000 000 0000000 100000000 1572 85173 1234003 20 000 572 85531 P2234003 40000 _ 1579 85183 P1234004 40000 P2234004 80 000 1294006 40000 2234006 80 000 P1234010 20000 2234010 4000 PI3403 40000 P2234013 80 000 _ 2400 40 000 2234020 80 000 1570 85191 P1234035 20 000 579 85541 2234035 40000 _ 24056 40 000 P22340536 80000 P123408 4000 P2234038 80 000 P1234000 20000 P2234040 40 000 1579 85203 1234041 20000 P2234041 40 000 576 85213 P1234042 20 000 2234042 40 000 PI340 20000 P2234043 40 000 PI3405 40 000 2234045 80 000 P123409 40000 P2234049 80 000 577 85221 1234051 20 000 2234051 40000 1574 85231 1234052 20 000 576 85551 2234052 40000 1234055 40 000 P2234055 80 000 1234057 20 000 P2234057 40 000 1234062 20 000 P2234062 40 000 1234063 20 000 1234064 40 000 B B 40 000 88 BB ds 8 8 80 000 80 000 40 000 40 000 40 000 80 000 120 40 000 40 000 40 000 40 000 578 85751 40 000 40 000 40 000 40 000 80 000 80 000 40 000 40 000 40 000 40 000 120 000 40 000 40 000 40 000 40 000 40 000 m 2234283 Membrane Protein 000 No 570 85571 0000000 1000000200 40
20. 0000000000000 eJ 0000000000 00000000000 00000000000 00000000000 000000000 21 Zmedium Control _ 33nM Treatment 67 100 nM 150 nM 230 nM 340 nM 500 nM Index Time hour 0 0 000000 0 H460 00 079 20 4 000000000000000000000000000 Poly L Lysine FN Fibronectin i S CICrI m M JI i 24 33 5H T 18 T Tem prar 29 30 siRNA 0000 0000000000000000000 29 40 Poly L Lysine Fibronectin 0 0 0 0 D 0 0 0 0 D D D 00 Wako Window 2005 No 67 00000 0000 _ 0000 un 00000000 581 84821 RT CES 16 0 00 16100 x6 31100 11500000 588 84831 RT CES 96 000 9610 0 H 12000000 583 84881 RT CES 16x0 0 00 161000000 55 000 580 84891 RT CES 96X1 0 00 961000000 120 000 050
21. 0000000000000 022000000000000000000 amp 00000000 03 0000000000000000000000000000000000000000000000000000 00000 0000000000 00000000 9 0 0 0 0 000 000 000 00 000 0 00 000 000000000000000000000000000000000000000 D DL HL UL UL D 0 0 0 0 2 0000000000000000 ww negtconm 0 0000000000000 O Gene Sweter 0000 200000000 0 0 0 0 0 60 5 20 22 90000 000000000 a 13 12000 00000000000000000000000000 173 500 1353559 0000000000000000000000000 00000000 OB ODD D 00000000000000000000000000 d trgi 000 12412 0000000000000000000000000000000 00000 50 00000 siRNA O O O L 0000000000 000000000000000000 ssRNA 5 0000000000000 0040 josai scoe 000000000 000000000000000 5 00000000000000000000 000000000000000000 00 00000000000000000000 00 0 Mn SIRNA 000000 siRNA 000000 siRNA 00000 0000 0 0000 OOO HOL 000000 siRNA 00000 O DH 0000 0 000
22. ar Heart Mitral Heart Papillary Musde Heart Pericardium Heart Pulmonary Valve Heart Tricuspid Valve Heart Ventricle Left Heart Ventricle Ric Kidne Liver Liver Left Lobe Liver Right Lobe JUS enum Left Lower Lobe Left Upper Lobe Right Lower Lobe Right Middle Lobe Right Upper Lobe Trachea Node Gland r iririr ririr ul in n Lymp Maxilla Ova Pancreas oid Parotid Penis Peripheral Blood Leuko Placenta Prostate Rectum Salivary Gland Seminal Vesicle Skeletal Muscle Skin Small Intestine Small intestine Small Intestine Jejunum pinal Cord Stomach Stomach Cardia Stomach Corpus Stomach Fundus Stomach Pylorus Testis Theca Throat T Thyroid Tonaue Tonsil Ureter Uterus Uterus Cervix Uterus Corpus Uterus Fundus Vagina B 8 578 85251 88 B BN 888 888 888 579 85281 4 576 85291 576 85311 1 88 3 B 576 85431 570 85451 577 85461 574 85471 578 85491 571 85501 574 85513 88 i NBN 888 EH al m ir n i M in a 5888 8888 81888 8888 B 8 8l ul ind Mi i SERE 20 000 A B 1234171 B 8 T 222 i BB 8888888 8 8 ul B B 8 Total Protein Nuclear Protein
23. one5H4 11 4 1 0000 PBS 00 A en 200 241 02 eil i MDA Methylglycerol 1 Hexenal 2 Hexenal 1 Nonenak 2 Nonenal ET ET E E C Linoleic acid arachidonic acid Cardidiipin 0 0 0 32 HPODE 15 0000000000000000 d O O 00000 UN 0000000000 310000 0000000000 O O 10000 0000000510 2 Normal rabbit serum iin 1 BSA 381 1 I UL EI ET E 5 500 21 00 0 2 5 0000000000000000000 00000 0000060000000 00000 0 0 000000000 000000000000000000 DD uUi 11 Yoji Kato Yoshiaki Miyake Kanefumi Yamamoto Yoshiharu Shimomura Hirotomo Ochi Yoko Mori Biochem Biophys Res Commun 274 2 389 393 2000 000 0000000 307 15591 00 0000000000000000 0 0 0 5H4j 18 000 00010 000 0000000 Un u 00000000 W3 DBI MOGONP _ 8 0000 00 0 0 511
24. zm NN 2 am 559 70291 19500 11 w 1420 552 70281 19050 2 3 2 1 249 2390 0000000000000000000000000000 0 http vwwwe caymanchem con U TN e9 2992992992099299209920920992099209920992099209 920992020929 92029 00 0000000000000 0040 eiililllllu eiili llllu 0 Wako Window eillllll E mail chem cojp X 06 6201 5964 Wako Bio Window 2005 No 67 0 00000 L E Leukotriene A4 methyl ester ii Leukotriene B4 pae 0000 73466 12 3 0000 332 5 0 000 9 0000000 1 0000000000 BLeukotriene CA 0000 0000 3365 0 000000000 W Leukotriene DA 00097 O 0 72025 60 6 6258 0 00098 0 0000000 000 00 955 EA 0000 2065 0000 4967 0 0000100000 F4 0 00098 O CaSHzNOSS 4396
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