様式 C-19 科学研究費補助金研究成果報告書 - KAKEN
Contents
1. 2 2 1 E2 E2 2 E2 10 FCS DCC FCS PRF RPMI1640 37 5 C02 AI ICI182 780 E2 AI AI E22. Hre2 E2 2 ER ERGs Her2 ERGs RRGs 27 3 3 ERGs ER
3. E2 E2 E2 E2 E2 E2 E2 E2 ztro ER ERE E2 TAM
4. 4 1 ERE ERE ERE E10 FRE E2 E10 E2
5. E2 E2 TAM E2 ER 1 3 o CE
6. GAAGAGGAAAGAGA3 5 TTGAGGACCTCTGTGTA TTTGTCAA3 ERE 10 DCC FCS 4 6 1 5X10 24 ERE ERE tk Luc1 pRL TK TransIT LT 1 Mirus 1X10 mo1 1 E2 24 T PCR Ishikawa Sawano H HHUA A431 Hec1A RL95 2 H JHUEM2 JHUAS1 H MCF7 RNA ISOGEN
7. ER BRG ERE E2 EGF IGF 1 E2 SO E2 E2 EN
8. pCMV DsRed Express BD CMV DsRed pENTR 1A MCS pAd PL DEST pAd CMV DsRed pAd ERE tk GFP Ad CMV DsRed ERE No 3 E Ad BRE tk GFP 1 mn3 20 30 PBS 10 6 DCC FCS PRF RPMI1640 2X109 PFU Ad ERE tk GFP 3 37 5 C02 GFP 2 10 PFU Ad CMV DsRed
9. 5 6 Matsumoto M Sakamoto H Yamaguchi Y Seino Y Takei H Kurosumi M Sasano H Yaegashi N Hayashi S 3 Dimentional 20 9 4 Microarray Analysis of Estrogen Signal related Genes in Breast Cancer ERE GFP Tissues Anti cancer Research 29 2009 3971 3976 9 akahashi Shiga N Utsunomiya H Miki Y 20 6 20 Nagase S Kobayashi R Matsumoto M Niikura H Ito K Yaegashi N Loca1 ERE GFP biosynthesis of
10. E2 178 HSD type 2 mRNA real time PCR E10 GFP mRNA GFP 17 HSD type 2 mRNA E2 ERE AIs GFP AI 1 AI NM ES i
11. GFP positive calls GFP posiive cells M wo PT ma wtG No of Pationts 1 HE 4 WE 0 3 I 1 RE TOMENGGF PTE 0 EW ERE E10 E2 lt ER BRE E2 ERE ERE tk GFP oan E2 ERE GFP 2 Ad ERE amp GFP MCF7 GFP
12. GFP CF7 E GFP MCF7 95 g 5 3 Ad ERE tk GFP ERE ERE 3 ERE 2 Ad CMV DsRed CCI ERE 6 6 6 Nn 6 GFP peeive call 8 8 8 GFP positive cels es s10 550 gt 90 GR Laboting Index o PT cancer 3 MI P BRE BR 3 Ne E2
13. SuperScript III RT PCR FxTaq PCR ERa 5 CATGATCAACTGGGCGAAGA3 5 ACCGAGATGATGTAGCCAGC3 8 5 CCAACCGCGAGAAGATGAC3 5 GGAAG GAAGGCTGGAAGAGT3 Ad ERE tk GFP Ad cytomegalovirus CMV DsRed ERE TK ERE tk pRC ERE tk Luci pEGFP 1 GFP MCS pEGFP 1 FRE tk pEGFP 1 ERE tk ERE tk GFP pENTR 1A MCS pENTR ERE tk GFP pAd PL DEST pAd ERE tk GFP pAd ERE tk GFP 293A TransIT Ad ERE tk GFP 293A 1096DCC FCS PRF RPMI1640 Ad ERE tk GFP Ad CMV DsRed
14. ERE 62 DR 6FT al ERE PgR FR 47 ER PgR HH 3 1 3 1 ER Ad ERE tk GFP ERE
15. 5X10 E10 2 ERE 3 Cancer Res 2005 ERE tk green fluorescent protein GFP MCF7 E10 E2 ERE GFP MCF7 ATCCO ERE GFP Ishilawa Sawano HHUA A431 JHUEM2 JHUAS1 Hec 1A RL95 2 ATCC MCF 7 E10 10 FCS Tissue Culture Biologicals RPMI1640
16. 1 100 1 1 000 10 50 ng 3 ERGs PamChip 27 ERG ERE ES WR 27 2 a 4 lt 1 Her2 ER 2 EA rs 27 5 2 Her2 GFP GFP GFP i E2
17. GFP ERE E ER 2 E2 ERE E2 TAM BR i AI a 2 ERE 3 ERE 3D hybridization
18. AT 1 10 7 mo1 1 4 0 05 IX70 GFP I E10 i A qRT PCR Rn RNA ISOGEN SuperScript III RT PCR DX400 DNA SYBR 1 PCR 5 CTTC TGCGTCGTGTCAT GCT3 5 GGAGAGCTTGCCATG CATCAA3 17 B HSD type 2 5 CAAAGGGAGG CTGGTGAA3 amp 5 TCACTGGTGCCTGCGATA3 L13a 5 CCTGGAGGA
19. 2004 2006 AcKerman 1981 PBS 1 mn3 2 5mg ml 40mg ml BSA 2mg ml 1X antibiotic antimycotic 50ug HBSS 37 20 30 PBS 37 59 C0 10 FCS PRF RPMI1640 E10 5 10 24 109 DCC FCS PRF RPMI1640 96
20. 3 37C 5 C02 DsRed ER ERa ER1D5 DakoCytomation PgR PgR PgR636 Her2 Hercep test aRNA 80 C RNA ISOGEN Nippon gene Tokyo Japan 2 ug total RNA MessageAmp aRNA kit FITC UTP aRNA aRNA Fragmentation Reagent 70 C 15 aRNA Microcon YM 30 RNase DNase free 95 C 15 aRNA 3 PamChip
21. E2 TAM AI Gn RH ER ER EGRs ER ERE ERE 3 FRGs 1 ER ER ERE
22. C 19 B 2007 2009 19791132 cancer through the cancer stromal interaction MATSUMOTO MITSUYO 80400448 6 3 The estrogen pathway plays a 2 2 4 4 E 1 RR n important ro ein thee Es
23. J Mol Endocrinol 2002 29 175 192 50 ng aRNA 37 5 n1 7 5 nl 20 SSPE 0 2M sodium phosphate 3 0M NaCl 0 02M EDTA pH 7 4 5ul 10 SDS PamChip 50 pl1 hybridization Hybridization 3 FD10 1 40 C 150 FD10 Michael Eisen PNAS 1998 95 14863 14868 cluster tree view StatView5 0 2 Mann Whitney U test 3 Kruskal1 Wallis test P lt 0 05
24. Midkine and its clinical 19 10 5 significance in endometrial cancer Development of Cancer Science 99 2008 1125 1130 diagnosis and therapy for hormone dependent cancers targeting Tanabe K Utsunomiya H Tamura ML nuclear receptor 66 iikuraH Takano T Yoshinaga K Nagase 19 10 5 S Suzuki T Ito K Matsumoto M Hayashi S Yaegashi N The expression of Tumor stromal interaction retinoic acid receptors in human through estrogen signaling pathway endometrial carcinoma Cancer Science analyzed by GFP assay in human 99 2008 267 271 endometrial cancer 66 Matsumoto M Yamaguchi Y Seino Y 19 10 5 Hatakeyama A Takei H Niikura H Tto K Suzuki T Sasano H Yaegashi N Hayashi S Estrogen signaling abi ity in human 8 endo
25. estrogen in human endometrial carcinoma through 4 tumor stromal cell interactions Clinical Cancer Research 15 2009 20 4 26 6028 6034 Toyoshima M Tanaka Y Matumoto M 60 Yamazaki M Nagase S Sugamura K 20 4 15 Yaegashi N Generation of a syngeneic mouse model to study the intraperitoneal dissemination of ovarian cancer with in vivo luciferase 15 imaging Luminescence Published online 19 11 23 2009 Tanabe K Matsumoto M Ikematsu S Expression of Nagase S Hatakeyama A Takano T idkine in human endometrium and its Niikura H Ito K Kadomatsu K Hayashi disorder 66 S Yaegashi N
26. metrial cancer through the 19 7 27 cancer stromal interaction Endocrine Related Cancer 15 2008 451 463 10 Analysis of transcriptional activity of estrogen receptor by ERE GFP 6 assay 1n primary breast cancer and its 1 clinical significance 67 Annua1 MATSUMOTO MITSUYO Meeting of the Japanease Cancer Association 20 10 28 80400448 Tumor stromal Interaction through Estrogen Signaling Pathway Analyzed by GFP Assay in Human Endometrial Cancer
27. trogen signaling ability in human endometrial cancer and breast RR Liology of human endometrial cancer and breast cancer However there is not report that evaluated estrogen responsiveness of specimens from individual patients reporter analysis system for detection of estrogen signal activity in estrogen dependent tumor We established new primary tumor cells Our results underscore the importance of tailoring therapy to individual patients with and our assay provide a way to accomplish this 2007 1 100 000 0 1 100 000 2008 1 000 000 300 000 1 300 000 2009 1 100 000 330 000 1 430 000 3 200 000 630 000 3 830 000 ERE 1
Download Pdf Manuals
Related Search