AMRESCO Fluorescent DNA Quantitation Kit ( 取扱説明書
Contents
1. Fluorescent DNA Assay Buffer 10X DNA DNA 1 DNA 1mg mL 50 5 10 DNA 2 DNA 3 DDNA 1mg mL 20 5 Dye 1 H332581 0 1ug mL 1 0ug mL 10ug mL DNA Stock 100ug mL DNA Stock Fluorescent DNA Standard 1 mg mL 10 uL 100uL Fluorescent DNA Assay Buffer 10X 100 uL 100 uL 890 uL 800 uL 1mL 1mL 1mg mL2. 2mL DNA DNA 10uL lt 1 DNA 360nm 460nm 2 Step 7 DNA relative fuorescence units RFU 1 2500 A9149x 4 63684 gc Mo y RFU x DNA 1500 RFU 1000 500 0 100 200 300 43400 500 600 DNA ng mL DNA 10 500ng mL Sample 0 1 ug mL 10 ug mL DNA
3. 0 1ug mL Fluorescent DNA Dye 1 0 ug mL Fluorescent DNA Dye Fluorescent DNA Assay Buffer 10X 3 mL 3 mL 27 mL 27 mL Fluorescent DNA Dye 1mg mL 3 uL 30 uL 30mL 30mL 30mL 2mL 15 7 8 0 1 ug mL DNA Dye 500ug mL DNA 1 0ug mL DNA Dye 5 ug mL 1 HO9 A 10 15 20 360nm 460nm 1 7 2mL DNA
4. 100 ug mL Final DNA Fluorecent DNA Stock uL DNA Stock ng mL Dye mL uL 1 2 0 0 2 2 2 s 10 3 2 5 25 4 2 10 50 5 2 2 100 6 2 5 250 DNA 100 5 000ng mL 100ng mL 5ug mL Sample 1 0 ug mL 100 ug mL 1 0 mg mL Final DNA Fluorecent DNA DNA Stock DNA Stock ng mL Dye mL uL uL 1 2 0 0 2 2 2 100 3 2 5 250 4 2 10 500 5 2 2 1 000 6 2 5 2 500 7 2 10 5 000 06 6396 6616 03 3235 0673
5. qMRBSCO AMRESCO Fluorescent DNA Quantitation Kit 1B1558 KIT HH33258 2 DNA AT 360nm 460nm dsDNA 10ng mL 5ug mL RNA Fluorescent DNA standard Fluorescent DNA Dye 20 C 1 DNA 2 8C Fluorescent DNA Assay Buffer 10X 18 25C 2 Fluorescent DNA Assay Buffer 10X 200mL 100mM Tris HCI pH7 4 2M NaCl 10mM EDTA Fluorescent DNA Dye 250uL 10mg mL bis Benzimide H33258 Fluorescent DNA Standard 1mL 1mg mL DNA in 10mM Tris HCI pH 7 4 1mM EDTA 2mL 750
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