取説 - キッコーマン バイオケミファ
Contents
1. 2 20 lt 30 35 C 2 Pp 20 ng gree OFF PD 30 io lt 406 1 PD 302. RI
3. YES 3cm Pen AQUA Pen AQUA 4 2
4. 3cm PD 30 PD 20 Pen AQUA 1 2 Ek Pen 2 20 30
5. C 2013 Kikkoman Corp 20150301 2 2 1 2 8 25 14 30 5 2 20 2 8
6. AMP PPDK ATP ATP Pen AQUA ATP lt ATP AMP 0 15ml PD 20 PD 30 10 11mol I 10 6mol ATP AMP 1 ATP 10 18mol
7. F Pen AQUA 3cm v sN MC Pen AQUA ENTER C 2013 Kikkoman Corp 20150301 kikkoma ATP AMP Hygiene Monitoring test kit For water and liquid sample LuciPac Pen AOUA CAUTION Instruction Manual LuciPac Pen AQUA Product code 60336 1 Do not attempt to drink the kit reagent or touch it with bare hands or allow it to splash into eyes 2 Please make sure to read the precautions and instructions in this Instruction Manual before attempting to use the kit and exercise extreme caution when using it LuciPac Pen AQUA is a kit for testing cleanliness levels of water and other liquid samples using bioluminescence techniques using firefly luciferase developed with Kikkoman s unique biotechnology Applications This kit can be used to test cleanliness levels of water and other liquid samples Do not use this device for any purpose other than what it was designed for as a tester of cleanliness levels
8. T 105 0003 2 1 1 Tel 03 5521 5490 Fax 03 5521 5498 E mail biochemifa mail kikkoman co jp http biochemifa kikkoman co jp C 2013 Kikkoman Corp 20150301 2 20 lt 30 d 4 A Sa be Pen AQUA
9. Pen AQUA 10 ml 2 Mg PPDK AMP Mg 0 CO 1 Pen AQUA 20 5 100 ie 2 Pen AQUA C 2013 Kikkoman Corp 20150301 1
10. Pen AQUA ATP AMP ATP AMP ATP PPDK ATP AMP ATP
11. Pen PD 30 PD 30 Pen 2 8 C
12. see Table 2 until they have reached room temperature gt Stick holder Orange Sampling stick Red A Container for releasing reagent Luminescent reagent Dee Pull the sampling stick A out of the main body B Be careful not to touch the sampling stick OS ae n Pull out the sampling stick slowly Put the sampling stick A back to and straight up from the sample the main body B and push it through all the way by putting the tip of the test tube C on a palm of hand or table Be careful not to get fingers caught when push ing it Gently shake the whole body of Insert the whole body of the the LuciPac Pen AQUA so that LuciPac Pen AQUA into the the luminescent reagent is measurement chamber of Lumi entirely dissolved tester And close the chamber cover Approx 3cm wM _ Vv Soak sampling stick into water or other liquid samples and shake it gently Make sure any bubble don t remain in the comb of the sampling stick Shake the whole body ofthe LuciPac Pen AQUA a few times so that the liquid in the capsule falls into the test tube C Press the ENTER key Results are obtained in 10 seconds C 2013 Kikkoman Corp 20150301
13. of water other liquid samples or other application which is instructed to use by the manufacturer Please be aware that this kit cannot be used to test or identify specific types of pathogenic bacteria Water and other liquid samples may contain ATP and or AMP which are caused by improper cleaning of facilities or originate from microorganisms propagating in such samples Since this kit can measure ATP AMP level quickly small indication of the defect of cleaning process or inadequate microbiological control of coolant or other industrial process water can be detected promptly This kit shall not provide any guaranty that a given test sample is free of bacterial contamination This kit is not suitable for microbial testing of originally ATP rich beverages such as fruit juices Measurement Principles This kit uses an enzyme cycling method based on a combina tion of luminescent reactions from firefly luciferase and pyruvate orthophosphate dikinase PPDK This method produces a given amount of luminescent that is proportional to the amounts of adenosine triphosphate ATP and adenosine monophosphate AMP present Pyruvic acid Phosphoric acid Phosphoenolpyruvic acid PPDK AMP Pyrophosphoric acid Luciferase Luciferin 02 Oxyluciferin CO2 light Fig 1 Principle of luminescence method using the enzyme cycling Firefly luciferase emits light in the presence of ATP and luciferin The AMP produced from this reaction is con
14. 2 3 20150410Q 20160709 NERKA Pen AQUA
15. F High temperatures may cause product performance to drop Table 2 Appropriate measurement temperature Temperature Temperature Model compensation range setting Lumitester PD 20 20 35 C 68 95 F OFF Lumitester PD 30 ON D 1 Temperature compensation is performed in accordance with the measured value of athermometer incorporated in the main body of Lumitester PD 30 Consequently the compensation cannot be performed precisely if the temperature of the main body of PD 30 is different from LuciPac Pen AQUA Please be sure that temperature of the main body of PD 30 and LuciPac Pen AQUA become same before use 10 40 C 50 104 F 4 Wherever possible be sure to use up all sampling devices from a single bag that has been opened at one time If you absolutely must store leftover sampling devices once finished with a test session firmly close the aluminum bag and store it in a refrigerated environment 2 C to 8 C 35 6 F to 46 4 F High temperatures may cause product performance to drop Do not subject the kit or any part of it to direct sunlight for long periods of time Strong light may cause product perfor mance to drop Do not touch any of the parts inside the sampling devices particularly not any part of the sampling stick itself with a finger or other object before use Touching the parts may affect cleanliness levels making them hard to determine Do not drop the kit or any of its p
16. arts or allow any parts to be struck or jolted The inner aluminum sheets and other parts in the kit may become damaged causing product performance to drop Do not use the kit if any parts become damaged such as the inner aluminum sheets Such damage may affect product performance causing cleanliness levels to fail to be measured correctly You can tell if the aluminum sheet is damaged or not by checking to see if the releasing reagent is leaking Q Do not soak more than 3 cm from the tip of the sampling stick into water or other liquid samples The water or other liquid samples which wet the shaft of the sampling stick may affect test results Secure the test tube of the LuciPac Pen AQUA to ensure that it does not become shifted or displaced It may be difficult to accurately determine cleanliness levels if the test reagent begins leaking because the tube has been shifted It may become difficult to remove the LuciPac Pen AQUA from the measurement device once the measurements have been taken Moreover it may cause malfunction of measurement device LuciPac Pen AQUA Designated Product for Measurement Lumitester PD 20 and Lumitester PD 30 Manufacturer Kikkoman Biochemifa Company Be sure to use only designated products when taking measurements Measurement Methods 1 Measurement procedures Complete the procedures listed below within appropriate temperature range as shown in Table 2 depending on an instrument used and temperatu
17. gular garbage but when disposing of it it would be better to separate the parts and dispose of each one properly in accordance with the local regulations outlined by the local govern ments for proper disposal of waste materials The main materials and parts used in this kit are listed below No PVC materials are used in the making of the plastics in this kit Table 4 Main raw materials of the structural parts of this product Polypropylene Polypropylene Sampling stick red part Container for releasing reagent Aluminum bag with Aluminum Polyethylene dehumidifying function Polyethylene terephthalate C 2013 Kikkoman Corp 20150301 Precautions for Handling Be careful not to let the reagents or other substances in the kit get into your mouth or eyes or onto bare hands before or after use Rinse your mouth out thoroughly with water if any of the substances get into your mouth rinse off your skin with copious amounts of water if any get onto your skin and rinse out affected eyes thoroughly with copious amounts of water should it getinto your eyes andimmediately contacta physician for advice and follow the instructions given 2 Exercise enough caution when storing and disposing of the kit and its reagents to ensure that none of the substances become mixed in with food and other products Be careful not to get fingers caught when inserting the sampling stick into the main body casing Please make sure
18. kikkomar Pen AQUA Pen AQUA 60336 N 1 2 Pen AQUA ATP AMP ATP AMP ATP 2004 ATP AMP
19. re compensation setting Make sure to always run measurement tests at the same temperature to maintain repeat ability from comparison if the temperature composition is not used Remove the LuciPac Pen AQUA from refrigerator and wait until they have reached room temperature see Table 2 Use the sampling devices them as soon as possible once they have returned to room temperature Remove the sampling stick from the main body casing Soak sampling stick about 3 cm from the tip of the sampling stick into water or other liquid samples and shake it gently Make sure any bubble don t remain in the comb of the sampling stick Pull out the sampling stick slowly and straight up from the sample 4 Return the sampling stick to the the main body casing and push it all the way into the the main body casing Hold firmly onto the LuciPac Pen AQUA casing and shake it Allow the leftover luminescent reagent to thoroughly dissolve Insert the LuciPac Pen AQUA into the Lumitester to measure the results 2 Handling of data Normal defect criteria should be decided by user based on observation of data for certain period of time under normal operation in consideration with fluctuation of the data In addition if possible the artificial inadequate cleaning conditions or artificially contaminated conditions should be evaluated Disposal Methods This kit contains no hazardous materials This kit can be disposed of as re
20. st reagent and the sampling stick required for testing cleanliness levels of water and other liquid samples Table 1 Main components of each reagent Reagent name Main component Luminescent reagent Luciferin Luciferase Magnesium acetate Phosphoenolpyruvic acid Pyrophosphoric acid Pyruvate orthophosphate dikinase Releasing reagent Surfactant Benzalkonium chloride Stick holder Orange Main body Sampling stick Red Releasing reagent Container for releasing reagent Test tube Fig 2 Each part name of the LuciPac Pen AQUA C 2013 Kikkoman Corp 20150301 Precautions for Use Please make sure to follow the items outlined below in order to obtain optimal performance from this kit Do not use products that are past the expiry date Expired products may not yield accurate results the expiry date is printed onthe label ofthe aluminum bag holding the sampling devices Be sure to use only designated products when taking measurements for amounts of luminescence This kit cannot be used with nondesignated products 3 The swab devices should be allowed to reach room tempera ture see Table 2 prior to testing if they are from refrigerated stock Measurement values may read lower than actual if the sampling devices are used while still cold Use the sampling devices as soon as possible once they have returned to room tem perature Do not let the kit sit out in temperatures exceeding 35 C 95
21. to store this kit and its parts out of the reach of young children Note that the releasing reagent used in this kit contains cationic surfactants benzalkonium chloride 2 Take precautions when disposing of this kit after use to ensure that such substances do not become mixed in with food products at food production centers and similar facilities 2 Benzalkonium chloride is a disinfectant and antiseptic commonly used in hand and finger sterilizer solutions Storage 1 Kit storage Kits are to be stored at a low temperature 2 C to 8 C 35 6 F to 46 4 F for long term storage The kit can be stored below 25 C 77 F for up to 14 days or below 30 C 86 F for up to 5days before opening an aluminum bag without any adverse effect on the long term stability Do not freeze the kit 2 Werecommend that you use all 20 sampling devices ina single bag at one time after opening an aluminum bag If you have leftover sampling devices that you must store after opening a bag be sure to store them at the recommended low temperature 2 C to 8 C 35 6 F to 46 4 F and use them within two weeks from when the bag is opened 3 Expiry date Printed on the label of the aluminum bag Warranty Kikkoman Biochemifa Company warrants the products in this kit to have a certain level of quality This warranty guarantees that Kikkoman Biochemifa Company shall replace defective products should any be found This warranty does not pro
22. verted back into ATP using PPDK to enable a high but stable amount of luminescence to be obtained ATP is a source of energy necessary for various forms of life that is present in organic residues such as microorganisms food residue and biological substances that originate from other living organisms This method allows you to measure and detect organic residues and microorganisms at high speed and high sensitivity by detecting ATP using luciferase which is why it is used as control index for cleaning process and for microbiological control of coolant or other industrial process water In addition this kit can be used to measure amounts of not only ATP but also of AMP which is produced from the break down of ATP enabling itto be used to test for an even wider range of organic residues The sampling stick of this kit can collect around 0 15 ml of water and ATP and or AMP can be measured from 10 mol l to 10 6 mol l using LuciPac Pen AQUA combined with Lumitester PD 20 PD 30 Therefore the minimum measurable level of bacteria would be 104 cells ml which is calculated from said ATP content of 10 18 mol in single cell of bacterium Please be aware that you cannot detect very low concentration of bacteria by this method Contents The LuciPac Pen AQUA kit comes with five aluminum bags each containing 20 sampling devices for a total of 100 sampling devices This kit is a simple integrated testing instrument that contains both the te
23. vide any other guarantees Kikkoman Biochemifa Company shall not be liable for any damages including special or consequential damages or expenses arising directly or indirectly from the use of this product LuciPac Pen AQUA Symbols Used in the Packaging and Labeling of this product Symbol for temperature limitation The upper and lower temperature limits will be indicated on either side of the symbol Please store this product at the indicated temperature range Symbol for Caution or Attention for use Symbol for Lot Number This symbol shall be adjacent to the manufacturer s lot number e g 20150410Q or description of its printed location Symbol for Use By This symbol shall be adjacent to the expiration date expressed as YY YYMMDD e g 20160709 or description of its printed loca tion Symbol for Consult Instructions Manual Symbol for Manufacturer This symbol shall be adjacent to the name and address of the manufac turer LuciPac and Lumitester are registered trademarks of Kikkoman Corporation in Japan and other countries Manufacturer Kikkoman Biochemifa Company 2 1 1 Nishi shinbashi Minato ku Tokyo 105 0003 Japan Phone 81 3 5521 5490 Fax 81 3 5521 5498 E mail biochemifa mail kikkoman co jp URL http biochemifa kikkoman co jp e C 2013 Kikkoman Corp 20150301 Direction for LuciPac Pen AQUA LuciPac Pen AQUA lt Leave LuciPac Pen AQUA at room temperature
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