No.9 1990 - 富山県薬事研究所
Contents
1. A C K C2. 4 1 C C IU C
3. amygdalin emulsin K prunaS1n penga1Qenyoe amygda1 1n ERRELE C 7 n dE RIAC EER RER JRR TEPRBOROR USERS IG Un t RO ESSE Heft RUESEMaduJgunteiseR AL WEA 8ucBXtLft amp t kadgEgToIRSe HERKEL SEERE ERRER EREA 1 2 min
4. C TS C C C
5. 18 X C A B B Atgorn 7 1
6. adrenaline adrenaline 1 fibufostero UED M C drYena11ne in Yitro
7. HOERUBIIGEE SEREK Ub 85 100 1 1 E 99 0 101 0 Z zB f xibu SERO 99 0101 0 85h100 50 24 99 0 101 0 A 4 Disa AiR uU E f m 134 E R 3 80100 80 5 38 0 gt 102 0 1 5 En FARY lomo Jota gt E amp UTUELOR m IO De iu 23 JIT 850 15 08 0X T Bak 5 4IBBSS ARBE ARLY AE imme C is24
8. 1 2 0 9 g 1 2 2 1 6 E C x 1 5 amp TPR SEEK BRHURUCPCKCRSAEDAA MA HON 2 1977 1979 8 5 1
9. mygd311n 1H6g 1 2 1 3 1 emu1etinC cinngmic 1dehyde
10. C 9 4 5 0 n 4 C d 5 5 18 CX SI 0 5 0 0D 2 8
11. S X D 32 54 2 0mg 1 0 0n DURRE CORTE L CREREESIC AS 1 C 1 D PUCLPEU 2 53H 5 1 A 908 1 0 0 0 8 0 Ont k vR 9 4 6 1 0 0 0 2 0 0 PH6 2 B 1 7 5 8 4 1 0 0 0
12. Rig 2 5 2 mt 254 Pre Coate TLC Plate JE amp 0 2 5mm E l 6 Un c0 79 uw ct Ls TORRE FiB 3 C IN 5 0 4 16 2 31Hg 4 571MHg ESI M LS mEHg 9 on Pon m d 5 WEg 1 6 1 3 9 147 Fig 2 6 0 K 1 5 gg mede bic z 2 3 5 4 96D ENEICBIAE UL ROEE BUS OS m SEDE BIEREL 7e Fig 4 Ami EKA OCEM 1 0 8 180 E 147 1 Ps 145 7 m d VN 8 E tit Ps 140 7 ad T 138 _ F Ga A s 3 is ELI
13. 1 2 0 9 0 2 8 4 2 B 1 Winter C A et amp 1 Tregtren cf Adjuvant arthritis rats with amp nti inflamm amp tory drugs Arthr and Rheum 9394 1966 2 SUKBU R2 IM H 1ndomethacin 2 mentho RE OSLZE BAR I5 243 585 198 3 Salicylic acid ester 3 1 567 1986 2 4 M KATDRT et ali A Possible Role cf Prostagra
14. C 1 Winter To1 8D 172 2 3 8 FOA0 0 5 C 1 3 0 8 1 8 5 0mV 8 0 5 0 0 5 8 8 0 g zvy h TREL 8 h 3 h 5 3 1 8 5 C 1
15. 1 3 2 1 6 1 6 2 7 0 9 4 skER xx R rXeTic MODELS RER HPL0C 4 E i LC 6 AN FFT TER 3 5 HPLC 2 0 mg 1 4 5 0 7 7 5 1
16. 3 2 0 nszsr AAA E SCRE BRO 3 Ana1yttoa1 Parameter 5 1 0 0 2 0 1 4 1 0 0 me 6 1 1g 5 Table 3 9 9 6 0 09 8 E 9 9 0 8 2 1 1 7 38
17. 3 1 Table 4 K 1 1 AE JUD X PSUB MEE 7 872A Fig CRF 2 HPLTOC 9 9 2 1 4 C Table 5 PREKARA EmO dA ELT n D E83 r M lanae unan P187 BERR
18. 1 Table 1 5 0 105 1 2 4 HPLCMKC 9 0 58 3 2 Jv z Tabla 1
19. b MI picis t C C C A K C
20. K 1 C Table 1 RIC byt LC B E Sov x eR cni Bieis esos m
21. di 2 HPL CC 1 Dg 4 y tnp BALS hne Ia 2 AL S9 9 0 d 4 3 9 9 0 2 WRH o TAA ARDEL TEK 100gm 32bk 0 83g y A AGI 1 0 2 0 5 ED 87 Table 1 z 2 HPLC 5 Shimpaek CLC ODS B RER 6 MX 5em 5 tm 40 b 52 48 L2m min 8 VERE TANE DAS Tt v4vrz UHWDCWHEE S350mTizb 27 lnc100mgf tULr 4 BERHO TRA 3 0 0 mg 24 2 4 mage RECEN 27 vt 1 0 0 me 1 00m
22. 5 bufalin bufalinfilx10 g nl 427x10 l EL n 0 aea E BBC ENS A 1xi0 5 gn K4 4 2 1 3 m 8 1x10 6 gb 4 27x10 2 m ORE D 5188 5 e 6 HXGRAOSH Jj cock Alb HJ Re 67 o Ji bc bie 67 Ha sE e e Beating rate UE eS Mp3 m X Cr beats min Tenslon g A ge 804 704 604 504 Y AV 204 4 au 0 4r T 1x10 5 3x10 5 141x1074 3x10 1x10 3 3x107 1x10 ARAL QU 4m Lov 4080 42 OD OLET ELT U IL AQ COGPEGERGUE NT d8127 Tn 1 re Tov bd E2 HU E25UdsvrdmdrernsalinscecoKedHE 12273 445 FH c2 FH JE Bc LOS Hi RS d OE UAAR Em ZELAYA AWAI5B cvv13HOY4SE SEEmT 1004 9 304 804 t M VI e 8 504 404 4 304 P 10 a 04 1x10 5 3x1075 1x107 3x107 1x107 3x107 1x107 TAG Qi
23. 2 0 bufosterotdg 3 0 1 1 C1 0 0 0m PLC 1 100 0 60 40 1 0 0 0 KA resibufcgenin 8 4 0 8 0 0 nm 4 6 1 5 2 5cm CK 8 1 0 Am VIDELE WL EGK 0 bufalin CtnODuTE1H Y6S1DufOEg6ntun 8 muscone C is mugscone 0Cis
24. Cpa K Coa K K A Cba K Dsco se A Dsc Coa ZAF ORIRE 8sc K o
25. 1 2 4 Tablet AP 1 1 1 0 0 0 H Table 1 Lo G J K C 2 A ERRER 5 7 coe8jO3 6 t DRAT Table 1 EK 1 3 Tire A 60 0 B 600 0
26. HPT 0 HP LC OD8S Shimpack CLC fH 5 2 4 8 Fi 1 2 im 8 6 5 zw ot th4n0 22 0 mO REMAT TEIG 7c 5e RR 0 999 Fig 2 A B en que OVD 0 Wow aa o 2 So 2 DR z o E T 210 1 lt A n Fig l Chromatogram of menthol d campnor 1 1 n 1 acenaphthylene o a 5 1 0 LS 2 0 A slandard L sample Concentralion mgZmi 3d camphar 2 menthol 321
27. E 81 aaas E nag zo ERa LM SEES REuERZT22 ERI F ss Hanes nasg ERE HESY UVEXSES etc Fig 3 E 3 K l DERRAMA 2 LARZ h HBI KEANU RICH VT VECBAJIU BAERHREIO Y HC 40 RAM K 4 HC 40 HC 4 0 2 3 PC 9801 PC 9801 HC 40 RAM PC 98010 e 2 8 HC 40 9 5 HPLO PC 9 801
28. 1 3 PLEN LERRARKOE 3 0 0 0 MELE Et dug C 1 Bd 1 8 3 0 0 0 mg L7e HRR RIRA A B ERR h ERRE k ALR G Ttt Ce A ERREIAN E BAUR D E FEBR AULAE m AIC E ARER 1n 6 9 2 1 Fig 1 2 UV HPL 0 HER HPL0C Tab1e 1 RRHH 32 e R 3000rom X 5minj RNC Ao E d HxcRoHAGER EA 1520 sa M Lies ps 50mt 5min Fig Table 1 HPLC ja AM 210nm Hz0 Me0H 10 1 Prunasin benzaldehyde 210m Hz0 Me0H 3 1 paeonitlorin 240nm Hz0 Me0R 3 1 i i h Fionentepidenyde 274nm Hz0 Me0W 1 1 paeonol 2 2 L MERCK 20 LiChrosorb RP select B 250X o 4mm 40 1 0 ob min 1 1E
29. Fig 4 C 10 bu 95 ou EX d 3 EE ST Vv 1 S eS 0T II Ei e 2 __ ____ H T 1 11 wed2 14 2 E Tagen nM 3 3 Dw Mo 12 0100 EC 40 Se be Oo Ftg 5 2 26 Fig QUU GPL 2 0 9957 g D 1 8017 9 3 4 1 8004 a Fig 6 5 8 9993 g 2 lt 8 9941 g 7 1 0803 g 8 98 9970 g 9 9 9924 g EREE I S 12921292 18 0 9989 g 91 1 252122 11 0 9909 g 12 0 9967 9 9 RET 123 0 9948 g zU AX MM PS C14 0 9973 15 1 8911 9 Dow b No 15 1 0853 g 17 0 9995 g BU ETT lt 18 0 9843 g EONIGIN ZEE DRM Oz 1 MEC A TT ILU 19
30. 1 x C amp n Mn Cu C C Zn 8 8 C HPLOCC TR C D 1 1 p813 315 1986 2 AMRA LOREA FPS RR L ARSH 188 189 149 1986 3
31. TagD1e7 Fig LERF M 9 6 1 0 1 4 15 6 6 9 8 Q Table 8 Fig ZICm3 6 9 Table 7 zia 37A Fig 1 BUY 4 L 0T ERIS 0 B 17A 3 67A uju ex a uez 113 8 1 7 103 3 Sus B 109 4x 2 3 110 11 102 3x eol ajc nm 108 0 101 6 101 6x l15u DI 138 107 07 109 6 101 0 2 E 11613 109 2x 112 3 F 112 23 104 37 104 7 105 73 G 107 9 102 3x 102 1 191 7 F 113 343 172 108 2 4 02 107 71 4 90 103 6 2 352 992 H nuse naar 106 2 99 8x 1 103 13 106 108 4 106 7 i r
32. EH E REC ARRECA FOEAUESUEED SLT AR SEARILUICE ST AS ESCERPISUK 3E X 1 ZE RIAR ARPA AFE KURAR BARFA 20 1804 146 151 1887 j 2 1 6 2 1987 3 1 1 C C815 819 1986 6 8 4 amp EBOGSA Studies on Quality Evaluation of Kanpo Medicine NW Keishnibukuryo ggn Hu s R t HB 5 F Masayo FUNAKAWA Yuko TSHTDA Ruriko NAKADA t u B ow BE Miyako YOSHII Hatsuhiro NISHIMOTO Ichiro NARIKAWA Research Institute Toa Pharmaceutical Co Lt ERRE dL HRS dO C LTES ULL Re onto wEo ocbn TORA SEEK cpm UG AAE BAIL ASXEAMSA ALAR MRA RETRO WUEKOdUL D
33. it A E 8 0 BB OR IRR emp SEZ x i08 zn Hie CO BAGNO RSS 0HE 10 2 c 1 8 h Std Wistar ST 100 12 6g 7 0 cm 1 0 0 amp 50 2 2 hn 5 0 ARL bC REK AEE ZEL FIER 2 b 125mg 258S 0 m s
34. CEZ n a tle e al a k PIRATAS 4 5 i m wa eim a RU NM zu Tem 3 ERUS RE AB oyn i OLCH EERE MEA Ol Witev j 3 TAS DEA K ELUA syy i HPLC 9 8 3 o EHRE 30 kia 10 n o 2iN ook MORS 8 Jai i 3 4 i 1 ONLCES UEM AMBAO c l ra 3 n nuncii o 8 I i Lr 10D iu 10 ici 1 ARR TH i Yoox AEAEE uc TREE RE l DAR KU Hu Biosy gmi kE OSEE mA lade WORGORSGUNUURA OUS RUSUR DU ER RE Ea i Table dtot UvA V v TOS LEG 68 z E t aa e EES aal 4 C Qi cn ld EF Fa Tab1e 2 E AK UE m i 1 ilica ge xt Silica te ilica zel 60 T LT C CHPLO pes i herck NM 3 P 1 as 7737 7K i neaeh K i C Los di je Bene pomen
35. CR44 HPLO POC 980 1 6 PC 9801 7 8 ASO1 1 8 2 1
36. 6 C 4 0 2400 bps C6 HC 40 PC 9801 1200 bps HC 490 PC PR201V2 Fig l 80 8 ig 2 7 8 E i 5 an TIMS I PC 98010X21 9 640KB 4 70707 37422 2HDX2 TURBO 22 208B RAM 722 1 54B HC 40 64KB RABS7 438 26KB 5 PC PR201V2 7 3 P4 HPLO 5 BRRR LC 6AD 8 BiU LC 6A Bk ERES C R4A 707t 37432 200X2 id C R4A MS amp Tx AE 200 RS232C
37. 8 0 6 4 M 8 1 8 BK D 1 8 3 4 0 0 1 g mL REE YU 9 0 2 0 1 5 g 8 0 KC 4 0 3 0 VC 2 0 1 1 5 8 DPL o KETER OME D 5 DH 7 8K 10 5 2 0 9 2 0 2 mw 2 5 3 1 5 3 C 1 0 0
38. 3 A ig 1 9 1 2 3 0 5 E Loi Lot 2 RE SERE Fig l 2 TL C IIPTLC b7 57 3 60 QUU TESI n 0 40 30 1 365nw 1 2 0 2 0 i AJNSEBACF A ERE IC O cc IX RORTSTSTEKKC Tig 2 X zy 1 3 0 2 0 8 Fig 2 ORT BEEKIE
39. BOSE XUI ERE 96 30 RR CERTE I GS pO SIE 20 D d 3 A KK Table 7 Table T 29 M3 S7 VISA 12 5 33 0 A 37 5 2 f UG Pig 2 Wmolte usps A 4 9 Te d C tE AU 5rin 1 0 rin _ Fig 2 a o b 25 50 ms X2 A S eus DO c2 h 30 5 10 40 3 3 X Table 8
40. ENT 10 i mwi0a amp l0m Table BIKTLOZXEUIKHPLCI OSR g amp alee teal pneu i ET TH TH M i 1 en vd GESI 1028 i 2 1 sa ma 0 lx wx lw aly sl oesdicHM iue REEL RATIS jain MENS KEPAN KEEN RIT 121254 T L OH OBPLOCH ER BEZA 45 17 RT RT FORBES Bic Ak mn j 2 EXT TED i m wn l jD 000 0 05 D C 514 X 1 5al min lal nin lal ain 1al nin Fo 16 CES 2 5ee g bi 1 1 a LC Table 3 PRERNA AREORA E 6 0 0 1 10g t3 5 REA o c OR Y nC IEREUC TE KC 1 0 0 oTLC Kiese1ge1 60F precoated plate 4214392 Jia 025m
41. 8 Table 8 93 za HORER zm 4 4 0 Table 9 Table 10 Table 10 RRHH ERRAR Table 9 RRHH EHA COH 3 3 3 3 0 0 6 6 E C
42. Ai H D 54 D cp B 6 Bc Bos 4 A as EIE SUR mom xm dw 145 7 v28BEm 2 2 2 Fi 2 MESORGSEENO OT YSL 8 E KR BR s 8 OTLCE ORPLCE OO Uk i A A d 1 SPOT 99 5 39 9 B HW 1 SPOT 99 7 100 1 PRECES 1 SPOT 99 4 99 9 IE TCU 1 SPOT 99 3 T os h Tabie 5 nod we Len amp x ss jz TC x DTLC HPLC K ise 992 ssi N t 1 seor 99 4 92 0 Wo iso 98 2 86 7 cw ue T qp rs 39 4 j 89 5 Table 6 THBUESEBURRIS SL SUAE n n E g g n 1 E se SE SE dac E NE ICE Pig 3 TIER GUUARUUSAD nD k 54 4 7 4 K 1 E 3 2
43. 0 1 1 0 Fig 6 2 1 0 1 0 0 C O C C KK Fig 6
44. ED DuTa1tn 1x10 ELLE cincbufagin 1x1 0 Eu EC 9 in 1tro bufosteroi Dufostero1d E3 1x19 9 E adren amp Langendorff iac oinobufaggin 10 C inet adrenalinsK H
45. 3 1 0 C 1 N 1 0 1M 500n m 1 118 1 7 9 7 EOE BK 4 A B 1 A 0 0 8 B 0 0 4 5 m mi RL REEL o 2 0 1 2 5 4 0 83 0 0 5 ww 3 1 C 1g CK 10
46. p 5 0 Ju1ly 23 1985 1 7 p i1 une 289 1989 Adm CATET S Fr RLORROCCONGAOSZEUCCUT LEE X No7 p 80 1988 1 6 p 27 Tuly 22 1987 Ne 9 63 1990 Studies on Standardization of Crude Drug Componentg l Berberine Ch1oride Division of Analytical Chemistry Toyama Pharmaceutical Research Association 3 Te BUSEQS Syunichi AKIMOTO Daido SeiyaKu Co LTD E OAGEX KARTI Takao SHIMATANI Kyukyu Pharmaceutical Co LTD o DEC Shigeru HORICHI Toa Medical C0 LTD Akemi NAKAMURA Tkeda Monando Co LTD Hisao MATSUSHTTA Tkedg Moha amp ndo Co LTD Youichi YOKOTA Toyama Prefectural Institute for Pharmaceutical Research C
47. Fig 2 0 5 5 0 1 8 ATE EHR 0 8 4 0 5 1 Fjg 1 4 0 55 6 1 8 4o sesRCHm A EN Au NA Ar U B
48. A SD 89 110X 100 5 X ERU E s ES J L EE sutrmmut annmnus xeu E OW Fig 10 X 3 1 kib MERA ZAHA RAFE ASAF FS 6 BISE I 1 16 1124 1985 2 2 176 1162 1986 aew EMES HRR AFAR EF 8 19 74 1988 4 TIMSITURBO Ver2 01 5 Ver8 6 CR4A MS DOS 7 CR4A BASIC 8 HC 40 BASTC 9 N88 BASIC 86 M8B DOS 5 09
49. 0 5 pe pop pe WW xd dut LI V Clcec3cs C5C6 C7 C8C9 22 cinobutagin 2 X AFARRARI Lg Am 1 TRUR ORED 5188525 1 15 R Litehfte1Q and wi1ooxon wk DED 3 C 221x10 95 1 0 5 4 6 4 170x10 EL m2 95 0 81 3 57 adrenaline 285x10 E mb 95 PERRI 1 12 4 94 adrenaline E 0 05 QED V DNS0 Cl 1x10 10 cz 3x10 10 C4 3x10 9 t5 1x107 t6 3x10 8 CH 1x10 7 ta 3x10 7 59 1x10 6 t10 3x10 8 DIET C12 331075 cinoltufsam inno tdi uc Uer 5 0 R3 AUABIUHRER 8207 HREORENEISH S RIED ER LUREREIEROE D o m OE Dy E D ARE 95 sgROgx SVEMIRA teanas ABA 2 0 t a 6 26x10 tt al 1 05 4 84 3
50. 9 5 5 Krebg Henge1eit 837 0 5C NaC2 118mM KC 4 7 mM OgC2 2 55mM MgSO L18mM KH PO 118mM NaHCO 2 4 8 8 mM 111mM 080m t li L icorgesn bath LEMO tgometrto transducer TB 6 5 2T EF 601G iscmetric C AT 6 0 1G 0 5 L 2 D gdrena1ine drena11ne 0 8 4 1 2 8 9 2 bufosterotdas tbufosterotdg bufa1in 1 87 citnobufggtn 46 9 resibufogenin 2 9 1 9 4 7
51. 1 0 cm 2 134 HPLC O 1 oHPLC 2 7 2 3 6 5 nm 2e 5 um 462m TDX15cm IAE 3 4 5 um Tnertsi1 ODS EG 4 0 6 mM 1 1 1000 2 1 5 Bi 1 2 1 5 KK 1 0 OMERA 2 0 1 5 EBR 0 ACRA k CRET SED T 64 10 2
52. 4 0 E 83 17 8 5 2 K E 2 0 nf ROXERISE T 38089 kk pH 3 0 2 1 5 9 6 1 REX 9 9 0 E m KIESELGEL 60 F Merok RBS 7 2 1 12cm REGA l1 0mp n 35m22 EREM 1 1 0 0 446 2 5 4 nm GOD 7B 1 2 tW 105 1 0 Rf 0 2 nE Renar L RABURROSEEEDOOnUBENEENOx tee NOR i trc
53. 6 Rf 1 0 c 0 5 e E e w 4 5 1 2 3 4 5 Fig 4 Fig 5 OZKO AE 3 RFR Suat Zr DLE 3 4 v VRBES B n 7 9 7 rv T E v e RIRA TSKgei Amide 80 BHH TORLI 8 10 REEN 2 Mva vyz Fig 6 8 5 AREFE Braki RSE gt 3 1 1 SELEN n p 0 ax 50 TNT pupi L 5 ES WHO UN nU EN NND E Tl n TED TN TP qs a Ju Su WINEKUMBER Em An SPCETAISCEPE Gu Li m Fig WULUBTN ym FEFCEIT 1 i BINA TG mu um wn mu m B L3 a o a ET NON a EU ten Leo HAN tostoc GL HM
54. 7 1 1 K P1g 6 BEC amygdalin prunasin mg ma snol 20 3 O anol Ld 407 7596 0 S4 3 D 6 months gc benzaldehyde ia51 cinnamic aldehyde as 30 s 3 15 A AJ 30 7596 RE months T on ns Fig 3 EBAGRSAEURIEBUEX SET OIERHEOOEBZIE I ns 18 8 Av RI a FE M 4 40 75 C 40 75 5E HAR paeonol paeoniflorin mg 10 Ji 2 Dp ae oue PEE 3 6 months montas Fig 4 HEBRERSIRU EBESAZSGOREBHEASOBRBZ C mo 1B8 m Table 2 1 mg mou A 8 C D E as z EER ERR EEK ERR u TET BHAEXE LETT SHE E A 600 1617 1200 360 800 4000 2010 R F 600 1617 1200 360 800 4000 2010 HAR 500 1617 1200 360 800 4000 2010 600 1617 1200 360 800 4000 2010 z 600 1817 1200 360 800 4000 2010 1800 4000 t 3090 8083 6000 5800 20000 10050 z A A Al a y a Irt 15288fu Iri IE If 400m
55. HPLC d LC 6A 0 RR8 A YMO A 812 0DS8 VP3j8 6 amp amp 1 5cm 40 5mM pH5 0 68 382 0 8 mtn B 1 1 KK 840nm l FE C 1 C C
56. t M ld d vas i 3v 7 8 TC 053 p eas OC 33 A CDC ig 4 8 et DG REUS Sue Hf T LC Fig 4 5 TLOC Fig 6 PIC fy ya 3 5 92 TR Pig 7 8 OH3 8370cm CH 1460cm Go G ni 1 1 i125cm 1104cm 1000am Cio i 2 Bb MM CAR vsat amp UdETEPROSOCP2ctREUZERKCS U bons MEOR EE CUT 5
57. 1 8 8 4 5 9 EB e Oc pg BR ERE RUOTE 2 GEZ 95 X 100 PO EREN Xm pmm n 1 BERSTOTSSEX x 1 0 0 RARER O Stop EB RO FERME ERE 02 V C FUEISRUESRUE EM e C Sp 8E SPESE RUE 3 C 8tQ Wi gtar ST 1 9 5 2 25E 1 9 C amp rrageen1n0 3 8 h C 1 0cW 1 0 0 mg 8 0 s HERL ro Carrageeniniz amp S nci 2 n R
58. Pig 4 T o 7n 6n 30 120 Time min Pig 3 Besovery and d camphor s Poa stnatol er Or mentho 2 aper 1 0 0 2 6 88 El D 3 4 8 K Table 6 HPTO 2 db G O T HPLC K TR ble 7 4 Fig 4 Chromatogram of CK Table 6 Determination of menthol and d camphor in preparation by HPLC and GC 10 958 E EDL 6 G c Sample Nos menthot d camphor menthol d camphor
59. adrena1ine 1 0 Bransontc 321 10 5 0 0 5 8 gdreng1ine C HPLC BB adrena11ne AGQ 812 6 X1 5 om pH3 0 0 1M sodium dihydrogenphosphate 0 1mM BDTA 2Na V 1W2 amp min 525288 40 C FL Ex28 5 nm Em305nm 20 A4 2 bufosteroids 10 0 1 2 0 6 0 1 1 2 0 m6 1 0 1 1 C1 0 0
60. Table3 E M H Shimpack CLC ODS ns12 n12 Bil 8 uz Bin RE h Js 95m 9 2 99 5 I ORDRE TAS Fig 5 4 D CC H PT 0 9 9 0 2 9 9 0 8 3i 2 RECARTE At LedUSERIEQS RIEUEIEER IC E cO ZESc b CbebREEX 1 MBES 2 3 4 5 101 p822 1981 1 4
61. 1 0 0 M 6 9 C 1 0 0ni LRSAL LA COREEA 0 UL HPLC 0777 HELC 4 Shim pack CLC ODS g amp amp amp 6X150 40 HE0 ERE b odd oi 2 100 89 11 81 bic E LOmwL min 4 5 0 nm 6 HPLC 1 8 EPLC K LT TLO 1 0 0 5 0 1 RAe ERT 2 C 1 0 2 0 4 C TLO TIC HPTLC Kieselgel 6 0 Merck HA 10 10 1 REE i 0 8
62. 90 n 23 1 D 551 556 Ci98se 2 USP p958 959 USPC Tno Rooky111e 1985 8 BP 1988 p501 504 Her Majestys Stationery Office London 1988 4 BP 1980 p8398 400 Her Majestys Stationery Office Londou 1980 5 RER EARS LU 2X JnEERS ICKGUSCEGHEQOSUEAER SHTD 4 9 amp 267 284 19 15 6 K 5 4 5 5 1 3 2 3 1981 7 Sagar K Oshima T and Yoshida T Rapid and simple determi 9 5 nation of sennoside A an B in Rhei Rhizoma by 1On Da1r high performance liquid ch
63. 4A GO TRGPEHA IE ORT mo L 7 mov BGNBELEGCDOMGAGUIZdSvk4 a doro e np a i n entr ZETE DA ELIO ARA JE OFRAR Eb 1 2A 15 1 3 SE 2004 350 s 250 4 Imin 10077 ioo 1x10 5 3x1075 1x10 5 3x107 naci ES bufa inp wv kdus bufalin 7 2 ASEPSEGERIEIS g ml 4 9 7 WR 6 cinobufagin ci obufa amp in sz10 79 g d 5 12x10 72 n 3x10 g ni 512x10 En 9 dk 8x10 g nib 512x10 m Beating rate beats min 3 Tens ion g Eo
64. T L Oo C 4 1 0 1 0 Vult AR LIBE EELA 0 2 me 0 8 1 2 0 0 83 0 1 g 6 me Dn 2 1 4 1 0 2 z 1 8 0 1 BA 1g 1 0 6 HPLC Es 2 C
65. ET E 1 C DIEM 3 mg 158g IC RC MC da y W pU 2 mg 10z 2 R 3 mg 15g Wistar 8B 1 mg 5s RR Lims a 19 05m g 95 252 1 4 76mg 3 E sdrena11ne bitartarate bufalin 77 2 cinobufagin 77 4 carboxyrethyl cellulose sodium Sa1t CM C eRT direthyl sulfoxide DMSO sodium chloride pot amp ssium chloride calcium chloride 2H 0 potassium dihydrogen phospheate mragnesium sulfate 7Hs 0 Sodium bicarbonate dAExtrosg 4 4 anhydrous 4 0 1 CMOCK bufs11n cnobufagtn D4SO CARL k 1 C 1
66. 3 2 C 1 6 KA C 808 5min 10 min 3 E 3 KK 8 5 40 7 5 2 3 HPLG T 6 GC HPLC ERP 18 7 5 25 1 8 minK 2 7 0 nr 8 GC 20 PEG6000 WW
67. Snima Ihei Medicine Co Ltd Talkyo Pharmaceutical Co Ltd E Z B5 Daito Koeki Co Ltd m Xx HO Toa Pharmaceutical Co Ltd E Toa Medicine Co LtQ Naigai Medicine Co Lt t X Fuji Medicine Co Ltg E 5X m Matsui Pharmaoeutoeal Co Ltd 1 R H Meiji Medicine Co Lt Toyama Prefectural Institute for Pharmaceutical Research 5 2 sA m Gb pe T Eiko ISHIKURA o Due Kozuo BNOMOTO Midori YAMAOKA E X Masao HIRANO 5 k Kinya SHIMA H o F Yuko HIMIZU s E RR X ko Fumiko ISHIGURO m 89g 2 Akihiko YAMADA A v s Hatsubiro NISHIMOTO x Jyunko TATSUMOTO 30 mg iW x Yoshie MAEDA B B Shizuko YOKOUCHI x X A Akito HAGIHARA Michiko HIRAI tkt F E Yoshihumi KITANO F Masako UEDA Tu i RASECIBNE CIANACUCESRSn 2p v FREH
68. 14 83 411 1983 8 8 A Hant amp nd M T Core J Liq Chromatogr 4 10 1869 1981 80 Na 9 81 1990 Ev lustion of the Quality of Oriental Bezoar R 8 ES Misao ARTSAWA Misuzu ISHIDA Youichi YOKOTA w LE x E E E X Chizuxo EJIRI Haruo SAITO Xx amp Toyama Prefectural Institute for Pharmaceutical Research m Bos taurus Linne var domesticus Gmelin Bovidae ORBO c ME RTLCDOBM CIAHPARURUdS eACBSSnhT OS A HP LC
69. 4 1 1 0 ZI 1 05 5 TLOC HPLO 1RC D 47 KEPRI 1 0 0 7 TLT 0 HPLT0C TI OC HPTLC Kieselgel 60 Merck HAR 1 n 4 5 1 KEER e KEC 83 1 1 0 8 4 1 1 Onl CELER 1057 5 5X8 o HPLC TSKge Amide 80 YV a 652 50m 8 gt rt0O 2 C 1 0 0 mW 1 0 8 0 Lk CNEAD Alt Zo kZ221 EEA r gt Dowe X50wXA4 EK L5X10o GBU KEK 0nl cHet2zuvtZ27t t te lini ostilue 81 4X KdBlbcU SUI forncudgerIas vro M dse 1 9 TL C HPLCK 1 1 0 0 7
70. 3 E 0 0 5 2 0 0 2 5 0 1 0 C 1 0 0 i 0 0 5 f 2 4 O 0 0 5 2m 5 RE LAEL to bAT i 2500 oGC 5224 109588S 100 87 1D 8m 100 1 3 0 60MAAmin 2 2 K N W Y 1 K 1 TLC 2 HPLC 8 C 8
71. 3 I 85 tn9 36 1990 Um x Preparative Exarinaticn cf Proprietary Indcmetacin Transderral Preparations B S ck BR E Teruo MTZUKAMT Yukari ARAKT Tomroak1 FUJTNAWA Deve1cpment Divisicn TKEDAMOHANDO Co LTD d AtS mu F Takashi NAKAHASHT Mitsue YASOSHTMA Ritsuko YAMAMOTO mo Jin iehiro INAGAKI Researon Division E 1 gt 6 0 1 RoiBelu ERHEOXEL RBUAE Cb JLOTIGBUD RI Gonzo 5nb l 1 dH OW W 8 S
72. XL XR 1 83 882 885 1963 4 H PL CIC 1 8 p80 86 1989 5 J A pl68 171 C1963 6 p10 1 5 1971 7 5 0 p844 355 1975 8 7 p96 6 9 6 7 196 5 9 4 p847 8 48 197 1 10 2 16 p98 102 1989
73. Ne 9 30 1990 Us oso zi Computorized Data Processing System on Pharmaceuticals Standard Tests Kazuo BNOMOTO Fumio TAKEUCHI Quality Control Department Kokando Co Ltd iE Bg H8 Yasuaki MASAKAWA Research Laboratory 1 Fig 1 Table 1 K 2 KEDY KA C 40 HC 40 t PC 9801 S 292C
74. K L4vFN 8 3 Fig 2 NII NA SN 1 SS ss C ss y 2 c zZ gt T x k An NA Ar U mlt B K LAZ F 2 TLCC oEFICASEHDE3SRICOwvc BBITEROTLC Fi 3C ER OovZTAHE VOUVOgES 245590 TLC TLC r RRE CEO EHE 8 4v FE XOoREDTIC HPLC EUI RK Fig 3
75. 3 muscone Cg 7 295mO0musconeli3 249 5 4 androstane 8 17 dione 2 fk X 0 259 52 ndrogstane 517 dione 0 14 1 narost 4 ene 917 d1one 0 2 8 8 2 AB 4 X 4 4 Tino R ursodesoxyceholio 2cid72 m A 0 34 E 1 25mg 1 28 57 383 5 chenodeoxyoholio 1 25mg Jeko 1 87 acid 3 6 5 2 TAY 1 25mg 4 69 dry 1 25mg 2 91 0 25mg 3 24 0 25mg ag 0 259 0 25mg a 0 141 0 25mg y 5 0 283 0 75mg 57 35 0 75mg 3 65 ajSa androstane 3 17 dione b j5H8 androstane 3 1 7 dione clandrost 4 ene 3 17 dione 46 2 DA dH A 1z10 A EL nOBEDOSEE i 71
76. TLO HPTLOC PT 0 HERR HPTLC Kiesslgel 60 Merck REGE Dn 7A2 ow KBRER TORRE 10 21021 10 10 1 HPLOZff Shim pack CLC ODS Et 6 X15 ou 40 8 2 1 2 100 6 4 2 97 8 Lonmin 4 1 0 nm 10 9 r cy C d ttc AERA ILASIBDASUES L ET OR U CUwvevARRUBIRSRTRD t Fig 1
77. 8 9 Re9 90 1990 Cassia angustifolia Comparative Study of Components of Various Parts of Cassia angustifolia Senna A R Misuzu ISHIDA Youichi YOKOTA Misao ARISAWA L A FEF E B X Chizuko EZIRI Haruo SAITO Toyama Prefectural Institute for Pharmaceutical Research H Cgss7o angustifolia Vaur Teguminosae 9 19 8 9 O 5
78. GEHE LEE 3 57mjox5g 30 1x 29 9 28 1 e 7 496 30 8 30 1 25 0 32 6 b LELEBAA AR GENZ HEERST 17 n eBECOGHEXECC 6 0 7 06D SEL AFEA bN 4 B C 17 2 8 A D 8 9 to Table 5 2 442 Y a ORRE EAA 5 0 2 2 5 2 6 0 7 8 12 50E 2 Table 6 Fig 1 Table 5 3 5 Table 6 5U LH8IBI E ng X2 s a nes aeo eni Fig 1
79. 2 1 2 A B A B HPLC C 4 0 Tc 4 B ey
80. 1 1 2 5 6 15 80 4 0 7 5 1 3 6 1g 8 RU Fig 4 C Fig 3 C 4 0 7 5 smygda11n prunag1n benzgg1aehyde 6 prunasin benga1dehyde C amygda11n cinnamic aldehy eti4 09 7 5 C 4 Fig 4 paeonol paeoniflorinK 8 AREARE ARAC T amp b1e 2
81. 1 93 8 49 101 4 102 8 A 2 102 2 100 2 102 3 3 100 1 0 101 7 102 4 mean 100 7 4 101 102 5 1 101 8 8 103 5 101 9 B 2 103 5 ad 102 5 101 7 3 102 1 9 101 6 100 4 mean 102 5 1 102 5 101 3 1 101 0 E 104 6 102 G c 2 103 4 8 104 4 102 4 3 99 1 07 103 8 1038 1 mean 101 2 4 104 3 102 7 QOO Table 7 Retention time and capacity factor Material Rt min K glyceyrrhiztc acid S 3 96 0 56 glycol salicylate 3 98 0 84 methyl salicylate 8 22 2 81 borneal 8 84 3 09 capsaicine 9 43 3 37 E nonylie acide vanillylamide 9 44 3 37 d camphor 9 62 3 45 thymol 10 83 4 01 1 0menthol 11 65 4 39 4 menthol 12 78 4 92 cineole 12 85 4 95 menthone 17 48 7 09 anethole 17 86 7 27 acenaphthylene 19 53 8 04 di K C
82. 5 C 2 5ml LEERME LA Table 1 Composition of Cataplasm A 3 0 5 s 0 To Lei 00g Ga0crm A 5 8 0 5cmX6cm C 5 MC 5 0 K 5 K 2 0 9 0 0 4 5 Zpm 6 E 8 0 g9 PTC 2 1 HPLO GC E ERIO MERE 0
83. 7 DOS 4 27v 20 R4A HC 40 PC 9801 NEN BASIC e JU C ERR U No Tig 3 1 Table 1 PC 980 1 ANU Kire v h SER HC 4 0 2 HC 40 HO 40 AM
84. 1 Fig 5 bA ER EF 1 C Fi1g 6 A B 0 tb5 DAUEHSEBI EDEEICUL 0c Pig 5C 1 amygd amp 11n pruna81tn beng amp 1dehyde A B C C cinnamic aldehyde CA BC 0 D CC D amp GOno cinnamtG gt1denyde paeonif 1orin
85. 1 500 0g 22 5g 180 0g 225 0g 360 0 g Lj 3 249 0g 1 529 0e 3526887 st 9 081 5g Ti 20 2 3 3 3 5 2 3 1 X Table 5 HAEL TAIRE k D 0 80032 y 4 0 3 2 4 5 kg e 87e Table 5 tatja x rw RO 5 2 720g 6 000 g 5 000 e 75g 600 g 750g 1 200 e 10 565 g 4 945g 145g 207658 30 0
86. 5 0 8 K 3 83 5 38 BUCG DS U B 4 85 c3 L t088s 3OtTIENKCED CnCnt D iisSCik 35 T10 3 0 1 6 4 0nW C A8 Ar Ao 3 K 9 mg D A0 AT o AS l Table 6 SHM ERIA MARR Q A m EEG HERA o 8 zn sz8 ezn 977 AAN e no x agel aryl Razh sel Lo il6 0 35 1 344036 110 106 Table 6 56 iss 1 2340 65 1 560 108 1 53 0 30 i 48 0 46 1 47 6 mmus 1 152m 1 132m EI 55 9 0 AKOE ZEE ho
87. HPLOC C 0D8 7 9 4 2 HPT 0 KC 2 1 c 47 168 1987 2 eje
88. 2 8 Table 1 g 7 5 mg 6 30 ag LARLL U 4A 7 5 ng
89. lt 6 minK K 222488 16 0 4 1 8 ENS T read ae 1 1 4 8 nh 4 8h
90. tn vivo ab ARAS o REEBUDEMEBIC MVC 1x10 EL mnEDIBBE D b ARREU ORMER b A 1x10 P MTRARDETL r dii 3x10 I E M EXE 5 nf COAT BRE 3x10 m2 5 bufogtero1d sdren amp 11tne bufostero1d tn vitro D 238 2 adren1tne E 98 3 dB EDS PA
91. K Je HEU JA ADARE SfEDODYOu RICILICESRSOBEDc EN MCO x Bk 1 84W8 5 EERSINIDEmIUR CtO0I UZT 285 E 1980 9 8 8 7 9 1 Nu9 58 1990 I Studies on Standardization of Crude Drug Componentg 1 Glyoyrrhiasieo Aoid Division of Analytical Chemistry Toyama Pharmaceutical Research Association BCrCS IBEBEN Yasuko ARAKI Asahi Seiyaku Co LTD BBIGSE uS Kiyomi UEDA Meiji Yakunin Co LTD 5T EERRER Hiroko KANEKO Jinsei Yakuhin Co LTD Hideko KAWANISHI Kerorinya Honten Co LTD Machiko KOKAJI Taiwa Pharmaceutical Co LTD C
92. HP L C 99 08D 2 9 gn 8 HPLC Table 2 CRT ERDRTa cm Bes 98 082 TT cE j EERBARE 9 99 0 2 nA ame E vn mab R gt G 0 EBDE E 1 ERCE Table 2 MEORE IRAH D 67 NOMNOROERER RH 254nn SEITSE AET ARAR 254m p PERELE L ME M cuc ore AEA ee p Imm cm Joum Hatars Size Lunroprep j EnA f 22 ABE 40 40 1450 psi 400 psi B 2XXMM DE KE EO RE DUKE De RD ViR SR x T mt min 3 5mt min T BERAE ngaio e NE Table 2 BE isot 1 M E Z2A tFigl md HP LC gt XA hE H E 99g meurt 1 Waterst voz BORM PEAT agamia gag 05880 DAE NM 5 Ir 4 5 7 5 TOY 0 SF 139 3
93. xRO z mun j Ga Corus 2 HPLC 0 RRI Table CRL tnicftoHPL jemma 3sT s2 ma 104 B A 7 I 39 7 OEOD2u ckZ22A0 f tFim 8 liam EE A 98 9 477 24 i 2am 3 48 1sROT 99 3 joas 98 4 emaj moi 2 HPLO 9 8 0 TLOC jt 5r xm z 5 s2 zj EE so ga m o aiso 99 5 faas 100 0 9 0 oy isor 9 7 9 s PRIN Table 4 BURG ONU 6 5 13 03 12 11 12 09 12 11 1 A23 Vv28HEmR 1 qEkogeik520Y 7340 es 12 14 2 98 S 65 que gt NR es 2 1 ANR 2 2 fipp2 2 6 6 12 12 58 34 2 9 WS t 2 RC Et
94. ae ocH v sOGBRAWGEAIES CX a d D EE Xo d tB C 505 0L 3 4m al V DREZEN EAB kU Sy TE ZE FH FA Ra PC E65 HB AR Eeo ail3BlOoT eWEISERmTY amp 177017228 BFEXIDuT5AWROR mITRLE Rl M2 M3 M4 a5 M6 M7 M8 E ES t E epa A9 Adrenaline Tn 11077 g m Mi M2 M3 M4 M5 M6 M7 HB M9 ERR APELA ZUR ALH RA E ER ZEB Ay PA gt i8 HASC BS SOS TILES CL 0p 37 S HOS a EERSMOTT IWABMERUHITOAWGOIL m10 TY I hb A3 A4 A5 AG A7 AB A9 A10 VAL A a dren mem adrenal inr Z 2 APER 6 m1 a l n e v v bk di HOD aE VT a 4 6 7 adrena 1ine C
95. dk E Mierocooous Lysodeiktious DARAI v ERREUR A A 6 A40nmCi 53538E2 10905 7z5Eb5 BCAA Y 4 2 oyyc EEBIE 0 1 105 2 2 omz RCE B ECI 0 0 5 CED URERA EHA TERENCI 00me ne ERCE V y RARE A4 5 Onte L y Y 3 2 0mg 1 0 0 0 B C 1 0 0 3 0 TEX 5g 4 C 1 0 0 nti A C 5 Ontel SUSHSHE DE 3 5 B 5 4 C 1 0 Om t3 5 CO 2 KC 4
96. 5 1xt0 E G 6 5 8 axi0 E oE 3 b du 8x10 3 5 1x10 DE UHRE AA bET RE 4 adrenaline adrenalinet 3x1 o 3 4 Al 1 87X 10 5 Rat oil o 1x1 g ml 617s10 7 nl 8xl 0 gn 187x10 E nb 8x10 7 gn C3 L87x10 Xm adrenaline BEED 1 5 CRF ERE KI Ze Ct AP UAE Met 10 100 i rem 80 S ONE Td so y f 50 d 40 30 1 20 SU Nd 10 E D 1x1075 3x107 1x10 3x10 1x1073 331073 Tygo
97. 7 N lh 24 130 2 23 23 45 1 5 mmilg 1 anii 1800 16H Leng 0 T T T AR 50 MUTO 0 10 2 3 n o min Fig 3 E1Sm 3 oH en Fig 4 dU TIVUT VERAS OR Edi fuEgogzublk c HPT CC ROTET AO040 C 2 HPLC HPLC BUE B T NI b 0 1mg uf mA 7 Wig 25 4 nm 4 6 10 8 0cm C 5 1 0 4 0 1 5 0 13 7 1 5 1 0 2 1 0 0gZC 2 0 4 CC
98. ATE 9 8 100kg E EE T ime amp n H 5 5 amp R amp x mom S m EX E RN X OM 8 X 0 9978g max 1 0559g 0 8196 E min 0 9843g 1 35 H E B8 B i E E PASA 90 110 100 5 x n 100 0 x n 99 7 x i j Fig 9 3 4 6 Sg 10 ox amp di annn XKWMEG H1 Ox E BXH CT SUAE m Dr KE FAH HPLC TT TT ue T NENADA mo 6 mex Arh Iaalle j EET a _ R nne 28A x 0 0978g E ta LOS 30 80 TET AT
99. C 500 0 D 7 5 E 60 0 F 75 0 G 120 0 H a mi iom 1 194 5 J 3 000 0 C D Table 2 5 PO 1 8508g C L 1 0 8 0 2 AR Tab1e 3 BE 52 HPO 1 bp O gMD x71 CIERL Tab1 e 4 Table 3 4 1 1 fXCCH SURE RC 1 5AL0 80g 5 HP Table 2 fru modo 216 0g 2036 1 800 0 amp
100. 3 c v 0 83 O6 4 A 2 2 4 2 WHER 2 6 0 min 92 1 00 Table 5 l 2 2 2 6 0 min 1 0 ADRI N A Fig 8 9 0min Table 5 Influence of solvent on analysis of mentho and d camphor yan solvent menthol d camphor 6 no methanol 100 0 100 5 ethanol 91 0 93 5 Ei amp cetonitrile 66 2 69 5 za Shake GOmin Each value is lhe mean of lhrec en elerminatlons OO 9 x a0 20 5 dk A o 8 5 Ay Z DA EO BARA cde RISUS e 82 UL 9 d
101. 5 4 0 PENAS A BEARDA n l 20 MEE K l S LL o ap so m 9 ao BP qme 1 EK 1 50 2 1 25 Fig 1 59 B AES X 25 4 nm 3 A i 2 02 15 3 0cm C 5
102. 8 0min 24h 48h Tb1e 2 Cft o TERREINE AJE X RW CI one x 100 Table 2 9752 EHE EE R BEEISORE 1 9 C A AEIR gokie EARR o H Y PaRa Fy sx KKE D Table 8 2 Fir Iir Ak Xk Par Fr k Table 8 Sy FYNY RAMAR G 5 0 2 gn 27U3A 7 HERIBEHO2 v FOMENT SERMO a SUSCI ERE zal mu za T es C 5 hn 55 57 A B ly w 24 6 24 0 C fto 20 8 0 2 D Tables 4 Table 4
103. 80 9959 9 28 1 8059 g 7 ET Ts 19 9560 9 VD 0 9972 9 T nadan TW 8 0900 g DAS g Eanp yam 8 8012 9 9851 g r 20 1 8859 g magiya 1 0000 dero 7639 8 0216 g T naldau 1 8818 g B 8118X XC 1 35382 afe XE bc 3 3 4 5 HPLO Fig 7 8 Pig 7 C tg 8 K un 31 0100 mW 2 44 tE EE Eee agno ns 1 RENXURC CH PRAO TINE AREA HEIGHT MK IDNO coc NAME 1 1 3 261 n5 55 2 100 1836 TERTII 4 426 3485 ELI 3 99 7484 MAND Pom Ano 38 a js ioca Se em io amne apra TOTAL 18137 102 300 2427 Fig 7 Fig 9 KK LE 2 rA ftd PurEg3
104. 9 101 1x 94 22 118 8 111 8 94 1 103 1 193 07 95 3 111 72 108 3x 97 03 116 31 118 13 101 5 3 116 2 112 1 96 5 3 111 97 107 27 95 87 113 6 3 67 109 74 5 86 96 643 613 6 Table 10 fO OS Sot meom jem on nas TELTE Te RIS ERA 744 v 10 2 1 62 99 950 653 98 7 dl1 E amp 217 17 100 8 3 25x 101 2 2 232 100 4x 7 101 1X2 12x 101 3 4 272 100 2 F 103 9z2 89z 103 7 2 013 99 8 12317 103 0 3 46x 103 3x 1 815 100 3 EeXSE UE D RERO oce 9 c H olftmi amp tcouwc Q lt 9 15 8 1 0 5 9 15 8 9 0 1 2 02 8
105. E Tt Fr iita m It i 1 e Hirini CH PKNO TE AREA HEIGHT MK 1050 cosc 1 1 3 259 319 531 2 3 425 3467 459 3 3 7 027 3854 385 R 1 4 9 6 8536 636 4 TOTAL 19039 3010 0 F s ba IDNO Nase Tipe Band Conc Factor Factor 2 1 z 7 02 1 2 TMUD Y 3 25 1 002 20993 3 LA 4 42 0 9967 1 10805 E iiM 9 6 1 6 451956 Fig 8 oyt PO 9801 HPLC r d ad end d No 232 0100 No 10 l bE RRES A 11 157 E S a 01 11 02 No 32 0100 No 21 0200 oi 11 02 SR MEABE 01 11 02
106. EH Fi amp 8 2 C 0 5 2 9 2 6T fc 4 Lot 1 Lot 2 2 84 SERI 1 75 Fig 3 B 4 B HPLC Pig 4 1g 5 4 B A B A B 2 76 a b 1 1 2 2 L cr 0 10 20 230 40 0 10 20 30 4 oe 1 c 1 2 2 r T T T r T T eg 0 10 20 30 0 10 2 30 40 Fig 4 2E Lot D 1
107. Fig 8 IR E UNES 4 4 gt FEET Am 4v CECRLOC mne rROBIPEUESSZzoso c amp isncoc KSo0tEt e cO Pig 9 1 2 Pig 90TLOXS5 4v VEECOGOOA amp xH rHaA4 vr Rf Pig 10 11 PELQGLb 1 PET A Hig 12 86 1 247FE amp E C R ShimrpackCLCODS 5 22100 itig 6 UV 410nm Fig 9 L4g wzro QA4vVVEWHW ibE AS 3 a BRUNEI n 2 7 ov OKBER ZKIRER 0 10 1 b WEW 7 3T v3 v x3 Ww T7 TX iR 10 10 1
108. SHINOHARA Ryuji YAMAMOTO Masayuki TERASAKT Jiro MORITA Toshiyuki MATSUBARA v in v1tro 5K adrenaline un A EIC n Ahna RRE SR SP CEZXucCL2S852 t7z5fcCt b5 AT DAGERICOwv adrena1ine bufosterotd El L E Ri HMCHOLATA CHE OUR z tomwu 4fnmoids ELE
109. Spp pods and leaves J Chromatogr 808 891 395 1984 15 A B K 39 1 06 110 1985 16 2 EGRE A B 3 6 87 92 1985 17 SEHR 80 129 132 1987 18 B 1 68 17 4 19 8 6 19 Lemli J and Cuvee1e J Transformation of anthronegilyoosides by drying of the leaves of Cassia senna and Rhamnus frangula Investigation on anthraquinone drugs XXI Planta me ica 384 811 818 1978 20 27 597 601 1976 96
110. xi on bon 1x10 K EPI 70 m SU 1 8x10 p 504 EL nO BO o Tail 8 nl D DO FE HER IC RB i3 nl DON 8x10 Rm zi nd o o o CB H E 1x1075 3x107 ix1074 3x107 1x1073 3x107 1x107 5 C fl C1 E z 5 v FREM AL OD PRERE 3x27 m 3 Too b fi BOJ AA I S iT D ATA ALAR tE EA I JU ES TE B tm A gt Bie Dc LU dB R UbosERISHORSEISERmT V CHC Ri 1x107 R2 3x107 4 R3 1x107 R4 3x107 R5 1x107 3 R6 3x107 2 R 1x10 1 y Ri R2 R3 R4 R5 R6 R7 A2 ARALO D e rdi 219i ECL Sea AWAOTZIASSEREER E n1 47 2 cw x d lx10 5 I
111. 0 2 1 0 5 1 5 2 1 2 8 0 0 2
112. 00 Tab e 3 i m A TRR 5 HPC L L4 SUN 450 0g 5 HPC L nm X x2 9 1 1 RE 360 0 526 HPC L 360 0 g USES 3 kg Table 4 om m aasam I GA 9 2 2 2 3 1 107 5 1 1 10 11 11 12 13 9 103 4 108 6 1 1D 9 40 1 1 5 LO 2 7 5 5 R H C 6 2 ivciBonfc ANC xT7 258 734377924059 9014c1 8 25079088 e 23L
113. 016 QRR OWM 1 B 2 A 0 1 0 2 0 3 0 E Se LE Lot 1 Lol 2 FE A B Fig 5 A B T5 sim C C A B NT UN A IIO
114. 120 mg S SS ORE 77 h qo 60 sg 20 ng 15 mg Z 2m 2 8 DRED 7w2 wDdSBRLCUA vFX2v zDE amp im Vu k S CARE SHRU L 1 gt F2AYr AARTE DANS 2 Y ARU BHES IA C Ta amp b1e ERT 2 E Carrageen1n PTONTN A SES B UE A 274 7c10mn mnoiECRBISEL 4 rzv z clREUx S0 FCACBET tTTPU7 2 amp 2 2 2 4 50 6 5 1 D X m x xm GUEEASEDETCLXC 2 REGERAT BAS EXeLLUO 2 btbTYUaevy BH WARRE r RERA ISSERRCALSJIdEETo L
115. 21 12 2 zyy 1 70x10 Apul SESZL 5 00x10 ri al HEEL 0 81 3 57 2 65 12 2 EAZL omis 1 s 1107 te ul s 0 thal 2 35310 t a ERZSU 1 15010 al FERL 11 12 4 94 10 44 2 99 777478 gt 1x10 Inu gt ixi gan gt 10 pu Co to pan a ED 00855 ZU Es BOEISU Leaf ieid and Wilcoxon 1909 b AA ES EEZLOC0 1 1 25m9 1 82109 23x109 5 8610 9 d k k Litchfield J T and Wilcoxon F J Pharaacol Exp Therap 96 99 1849 62 5x10 EL nL 9 5 221 122 570x10 5E nl 9 5 92 265 122 adrena11tne 11sx10 3 Aol 95 044 299 adren amp 11ne ED P 0 0 5 2 bu ostero1d
116. 50 amygdalin prunasin benzaldenydeO amp Fig 2 CRL Ai Hihid 1 smygda1in 2 0 min 5 0 1 0min Camygda11in prunasin RU benzaldehyde H amygda1in 5 0 5 0 K amygaa1in 70 1 8 0 min smol Me0H wol 50 Me0H amygdalin prunasln a benzaldehyde o lai 0 A 0 0 40 10 20 30 40 i min iB m min Fig 2 1 g amygdalin prunasin benzaldehyde wol 2
117. 81 1984 4 Re ERO db ERRE LO RAF HIRE 5 K Toriizuka Pharmaceutical Studi tion Used in Traditional the Components in Keishibu Pharmacy 4 2 1 8 9 Sino Ja 7 5 es on the Medicinal Prescrip 85 18 5 189885 panese Medicine Change of kuryOo gan Prepared in the Hospital 1988 9 76 1990 x HPLCiC E High performance liquid chromatographic analysis of essential oils in cataplasms B dH fe 75 Shin ichi MYOKAN Tsutomu TANAKA R oe E Kaori IZAWA Nobue FUKAI 4 OX ot Quality Control Division Teika Seiyaku Co Ltd CGC C
118. HE ER NEb l UCPAD BERECRION T AE 488 488 12978 1977 183 195 1 K XS 3838 817 824 1987 4 E IBRA KG ERE JILER 2 87 361 878 1986 5 cinobufagin 1986 6 269 292 1985 86 5 1 88 4 is 4 28 otnobufaggtn Na 9 52 1990 Stability of Cold Remedies mixed Lysozyme Chloride Division of Pharmaceutics Toyama Pharmaceutical Research Association amp Asahi Pharmaceutical Co Ltd k R X Kokando Co Ltd Kk Koshodo Co Lt m Geupei Pharmaceutical Co Ltd
119. LLL LLL S LL cs l 1 A8 15 nx 10 113 07 113 73 1 p n 6 K nex 103 5 107 73 104 5x 0 1 2 3 1 5 6 1 8 9 2 t nez 101 2 105 9 106 1x IM nig 109 73 133 3 111 8x xosuisiigemin3s4625 10 012328 107 94 6 54 Fis 2 WM JoOIUCHCWSM Tabie 8 0 B 1 3 67A 9 E eon i eas pee ea e 100 0 165 9 42 78 101 021 13 99 6 0123456789 0123456789 102 100 124 41 101 5 1 84 100 3 e Jis 102 024 40 104 021 98 103 8 102 854 21 100 745 47 101 0 107 423 46 99 421 91 2j 968 101 0 2 73 100 121 38 9 6 100 721 39 100 3 3 27 98 9 104 321 63 103 3 1 30 104 7 dl 99 62 21 101 0 14 100 1 Ma 4 ud 104 313 02 100 8 3 30 102 321 61 102 722 58 102 3 t ENA Bu itid 2 fI eC E 5 E RE Tab1e I Tabie 1 0 Table 9 mod oB Em m oB w m 96 116 2 115 0 99 0x 109 110 71 101 2 112 0 121 11 102 75 113 61 103 62 91 22 116 17 106 9 92 1t 112 2X 106 ix 94 6x 107
120. OBERLLIBE Rag x Hiroko KONAKA Uchiyama Medicine Co LTD EGET CIS Takao SHIMATANI Kyukyu Pharmaceutical Ca LTD gos XEM Hiroko TANAKA Seijudo Yakunin Co LTD 7 4 x SES Masaaki TERANISHI Teike Seiyaku Co LTD ARRA Miyuki NAGASHIMA Daiichi Yakuhin Co LTD HEK 4 m mSEPR Shin ichi MYOKAN Teika Seiyaku Co LTD BHE KAZAR Kazuhito MORII Daito Koseki KK rpm Naoki YOSHIDA Chuo Yakuhin Co LTD Youicni YOKOTA Toyama Prefectural Institute for Pharmaceutical Research CO ml 6
121. S Cacenaphtbylene Fiz 2 pinndard ealibration curves ot cam Oi menlhol ed camphor 8 4 G CC GC Tab1e 2 8 HPLC GC Table 8 PLC 5 C V 0 8 8 AT Table 4 Tabie 2 Analytical conditions of GC Table 3 Determination of menthol in mentha oil by HPLC and GC Detector Flame ionization detector Column PEG 20M 10 on AW ChromosorbW Sample No G C 60 80 3mmgX2m 37 2 Column temperature 130 5170 4 Zmin A 37 4 36 8 injection temperature 200 ST 3 Carrier gas flow rate Nitrogen 50si min 37 1 B 36 8 37 4 mean 87 1 Table4 Influence of repeated injection to HPLC on 1 35 6 analysis of mentha oil c 2 35 3 3 35 3 No mentha oil mean 35 4 1 36 2 96 2 36 0 3 36 3 4 36 2 5 36 8 mean 36
122. g 1000mg 1150mg amygdalin Cinnamic aldehyde amyadalin cinnamic aldehyde rr 20 anvedalin T 20 BE benzaldehyde 15 15r i A 1 o Ti 10 PS 10 S P v hi N e a 1 5 5 i S 1 zd A B 5 D Eg 9 9 A B C D E 1 Le Le Y i D4 A rta Di A izR ng mg UB 30r Ee 10 5 20 c 5 0 o E 1 BS A 8 e B A 5e B D E i LE lt l Ei A ITANS Di A Io oACBH Fig 5 mg NNJI P OTSIESPEDH IEEE CTIB Rm m amvgdali mg mg 38r j 3 2 15 BH benzaldehyde 7 gt 20 e 10 1 8 a a 0 a E 4 9 m i Lr RE A B C D vp S UDECCAW O30 uk l E I SRS ESE b EN I 8 A ITANN mg mg 25 50 20 c40 15 3 30 10 20 8 5 SO 0 A 8 C D E 9 A B D D E l l _ 1 A I TASbu 5 A rra m Fig 6 Ced 7 4 E VC TORR WAC bn TARAY amyg amp dalin cinnamic al ehyde
123. min 24 2 Fig 10 Q i4vFESSE NR Shim packCLC 0DS 97 3 Bts UV 410nm 5 123 min o 123 min Fig 11 8 7 RU o Fig 12 x400 EIU 5 fR Lj C
124. ndins and Brady kinin as a Trigger cf Exud amp tion in Ca amp rrageenin linduced Rat Pleurisy Agents Actions 8 108 1978 5 ARUm GABEEILAS TXTxbBS ES 190 2101977 6 7 8 8 4 8305 8 B Drug Tnforra amp t1on 9 62 288216 10 Theragpeut1tc Research 7 10 8638 1989 11 12 1986 p 78 88 5805 1989 D 149 No 9 44 1990 Us x Evaluation of Cardiotonic Action of Rokusingan Effects of Hokusingan on isolated atrial preparation of rat Divisicu cf Pharmacology Toyama Pharmaceutical Research Association ueg E Hircshi KAMIKAWA Tatsuc
125. romatography J Chromatogr 408 258 261 1987 8 Hayashi S Yoshida A Tanaka H Mitani Y and Yoshizawa K Analytical studies on the active constituents in orude drugs W Determination of sennosides in senna and formulations by high performance liguid chromatography Chem Pharm Bull 28 406 412 1980 9 Tanaka H Murata R Yoshida A and Hayashi S Analytical Studies on the active constituents in crude drugs V The struc ture of sennoside G a new glucoside from Senna Chem Pharm Bull 80 1550 1556 1982 10 Oshio H Naruse Y and Tsukui M i Quantitative analysis of the purgative components of Rhubarb and Senna Chem Pharm Bull 26 2458 2464 1978 11 Sasaki K Yamauch K and Kuwano S metabolic activation of sennoside A in mice Planta medica 87 870 878 1979 12 Kisa K Sasaki K Yamauchi K and Kuwano S i Potentiating effect of sennoside C on purgative activity of sennoside A in mice Planta me ica 42 802 312 1981 18 Atgorn R Weiler E W and Zenk M H Formation an distribution of sennosides in Cassia angustifolia as determined by a sensitive and specific radioimmunoassay Planta medios 41 1 14 1981 14 Due P V amp nhaelen M Vanhselen Fasstre R Hanocy M and Molle L Comparison between nigh performanee thin layer cehromatogr aphy fluorometry and high performance liquid chromatography for the determination of sennoside A and B in Senna Cassia
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