ZOE 蛍光セルイメージャー取扱説明書 - Bio-Rad
Contents
1. ZOE 32. Serial No 1 1 1 O mon 1 2 1 1 2 O WWW 2 1 O 2 2
3. H Fax 03 6404 0330 03 6404 0332 06 6308 6568 06 6308 3064 03 6404 0381 03 6404 0332 56 Appendix Appendix 5 F
4. A FCC A FCC 15 B FCC 47 CFR 15 103 c EMC A
5. Select X 11 A B MV Al 12 Settings 7 Delete all images Erect USB disk Export all images 29 Image management Settings Image management ZOE
6. RIENK ODRAZ OTIL BORAT ED BD REALE E CBAC IER FAX http warranty registration bio rad co jp registration htm Appendix3 4 il
7. 70 9 109 Tra
8. 5 PBS 2 6 3 7 E 5 900 1 PBS 100 u 1O 37 15 PBS 2 7 8 ZOE 49 MA 13 CFDA SE Cell Proliferation Assay Kit 13571201 CFDA SE 5 6 100 u g DMSO
9. 2 we a eee IN ii iii E 93 2 EG fea OED ee lt 2 ee eee Rea ee Oe oe SSR 4 iv US seesaivantan mown cA i Gh 7 SNS ei 9 TE DIA TIO eT GID RR veasepcdaaSucawnsase saa aeteagua ed TE 10 CO 10 2 ZOEW cocevoctiisccscesertssvesesseussvsevevsscestsaceseseerstveases 12 3 lt lt 16 OSORNO EEE NA A A E A E EE 7 E e E Eaa aS APE EE A AE NG 18 ET acess N A D E A A T E T 18 RIN I EG 19 ik 20 CO NN i 23 A i i 25 CA nN Nh Rs i 25 i Ue NE ea E fei i 25 TACOMA NE CCC eerie Ga 30 Moe A E N T E ETEA E ETE A E IEA E O E E S EA INA 31 OO 33 SN EVEReEee3888SDSRBEB3G20SSRO887888588D588039395esee Pe 35 ZOE CON EA ME Ne 36 i OTT tS ORT PON RT AAT Ti RPS OR em PINTS NCW 37 65 wae neath ard A EE E ETA 38 Software Notices and Term
10. E E a A ts a O S ABER ACT E xm ARO A7 VOPR E USB USB USB 1 E E E 1
11. E Gain Exposure E Gain Bxposure E LED Intensity Exposure E Contrast Brightfeild Edit Quadrants Q1 Q4 1 100 Contrast O 0 100 a OE 0 500 ADDY 5 Edit 21
12. BF YUH ees Save J Edit 13 Merge 14 14 Merge A B C Bdit Brightness Contrast
13. B BPAE tubulin jpg Merge 4 ZOE ZOE 36
14. JPEG Image file format TIFF RAW Color Serial number Time stamp Color Prefix Time stamp Hela Yes No Yes USB
15. No update file present 4 Enter 5 ZOE ce 3 Vision board driver Driver board firmware ZOE cell imager software Vision board driver ZOE cell imager software Vision board driver 2 5 ZOE cell imager software ZOE Cell imager software version ZOE System diagnostic test 38 3 Position Live Gallery
16. 8 1 Live 2 3 Edit Capture A Gallery 16A LEV Merge 16B 5 Live 3 4 aTeo T 6 Merge
17. Ea RaO BIRD Av TOALETT YVO p OM ZOE ZOE PC OS 1V EN61010 1 2010 UL Std No 61010 1 2010 1 CAN CSA C22 2 No 61010 1 12
18. 1 PureBlu Hoechst 33342 1 500 1 DI HO 100x Ahy Z u g ml 2 M 2 1 100 1xPBS 1 100 1x 1 2 1x 15 37 3 37 1xPBS 4 PBS 37 5 1 1xPBS 4 10 1xPBS PBST 0 1 Triton X 100
19. Merge 7 Bdit Brightness Contrast Save Gallery Merge USB 1 33 34 M16 A BPAE tubulin jpg
20. 15 Merge Gallery Merge Gallery Merge 31 32 15 Merge Edit Save 13 Gallery Merge X10 14A 14B 13 Gallery
21. Ee B 4 J eC 145 0031j1 USB ZOE 1 ZOE 2 ZOE 3 ZOE
22. ZOE 2 2M oF HL at PE WH X gt Y X Y iv il
23. 4X 0 70 mm 1 um 6 mm EA HDMI HEA i USBA us oa J a FLEET A AIT X 1 ZOE RAT YF ZOE 1 E 10 1
24. 1 IEC61010 1 200 1 IEC 62471 EN 62471 EMC F C C 47 15B A IEC EN61326 1 A FCC FCC 15 A
25. Gi Gain Gain FA A ZA MB ZOE i Are you sure you want to permanently delete lt lt 774 gt OK Cancel
26. To ZOE Green 2 CFDA SE CytoTrack Cell Proliferation Assay Kits 4 TR OR CytoTrack 3 CytoTrack Cell Proliferation Assay Kits Ordering Inforamtion ag a 135 1202 CytoTrack Blue 403 454 53 CytoTrack Yellow 2 542 556 135 1205 CytoTrack Red 628 643 632 640 135 1203 CytoTrack 511 525 135 1204 URL www bio rad com cfdase Appendix Appendix 1 Ordering Information ZOE 145 0031 1 ZOE gt ZOB USB
27. CFDA SE CFDA SE CFSE CFSE HA oo Se RRC IE CFSE 557 46 CAS 150347 59 4 492 nm 517 nm CFDA SE 1 100 80 60 40 Intensity AU 20 450 500 550 600 650 Wavelength nm 1 CFDA SE 0 1M NaOH 492 517nm AU 50 CFDA SE Cell Proliferation Assay Kit 1
28. JPEG 1 000 10 TAME 9 A B G D B49 Gallery 5 A B Cit ett ey AVI AVE D E 25 26 CO TA AF 1 N
29. Position Live Live 4 3 18 ZOE 6 mm X Y 4 X Y 0 0 Center 4 CREE
30. Pairs tional Pd 2 Merge Live
31. GUTI 10 4 Position UC 16 2 4 3 Live Galle rs k lt Merge QY ZOE 1 1 ZOE 2 3 A is OB UV LED LED LED 355
32. 1 PureBlu DAPI 1 500 1 DI H O 100x 2 1xPBS 1 100 1g g ml 1 2 1xPBS 3 3 7 10 4 1xPBS PBST 0 1 Triton X 100 PBS 5 Le 5 1xPBS 6 Lx 15 7 1xPBS 8 PBS 9 URL www bio rad com PureBluDAPI Triton Dow Chemical Company 43 Meise PureBlu Hoechst 33342 Nuclear Staining Dye 135 1304 PureBlu Hoechst 33342 Nuclear
33. nm ZOE 135 1111 VivaFix 353 442 Cell Viability Assay 405 Blue 135 1112 VivaFix 410 450 Cell Viability Assay 405 Loo liis VivaFix 408 512 Cell Viability Assay 405 135 1114 VivaFix 398 550 Cell Viability Assay 405 135 1115 VivaFix 498 521 Cell Viability Assay 488 Green 135 1116 VivaFix 547 573 Cell Viability Assay 561 Red 135 1117 VivaFix 583 603 Cell Viability Assay 561 Red 135 1118 VivaFix 649 660 Cell Viability Assay 640 PBS 1 VivaFix Dye DMSO 50 ul 500x 2 PBS 2 3 x 10 cell ml PBS 3 0 5ml cells assay 1 500x 1 1 4 30 37 C 5 CO
34. 1 1 NM 1 FP W DO
35. Shift II II ay fi Ee gu IE lt R 7 a wil i SSO 1 TTIII Bxport USB USB USB Settings Bxport all images USB Export Gallery USB Export Gallery ZOE Settings Attach m jpg single color images to merge Yes
36. BR 2 Settings 1 Settings 2 i Live Camera Gain Exposure time LED intensity Contrast Gant Bright Field LED Intensity 1 100 40 I 100 0 E R Conas imo Preferences
37. 1 5 20 CFDA SE 0 100 u g P 10 i CFDA SE DMSO 892 5 u L 200uM 1 pH7 2 5 0 5 u M 500 1 1 X10 5 20 3ml 500 wl AMA a Fe LET 200 5ml
38. E Gain Gain Exposure E LED Intensity Exposure Edit 1 152 LED intensity Contrast 0 0100 1 PI 7 Edit 23 X8 Live A BPAE MitoTracker red CMXRos B BPAE Alexa Fluor 488 phalloidin F Ex 355 40nm Bm 433 36nm Ex 480 17nm Bm 517 23nm Ex 556 20nm Bm 615 61nm P Cascade Bue DAPI Hoechst Marina Blue PureBlu DAFI PureBlu Hoechst 33342 VivaFix 353 442 Cell Viability VivaFix 498 521 Cell Viability Texas red ReadiLink 350 440 A
39. vE N te SA Vag aE ays gt gt 1 2 ReadiLink Quencher Buffer ReadiLink ReadiLink Antibody Labeling kit 2 2X50 u g 10kD 1 1 Lo ReadiLink ee o l eee de 50gg 1
40. Quench buffer 51 7 10 8 ReadiLink ReadiLink nm nm ZOE 135 1001 ReadiLink 350 440 350 440 Blue 135 1002 ReadiLink 492 516 492 516 Green 135 1003 ReadiLink 555 570 555 570 Red 135 1004 ReadiLink 594 610 594 610 Red 135 1005 ReadiLink 633 655 633 655 135 1006 ReadiLink 647 674 647 674 135 1007 ReadiLink 680 701 680 701 135 1008 ReadiLink 700 713 700 713 135 1009 ReadiLink 750 779 750 779 135 1010 ReadiLink 790 811 790 811 135 1011 ReadiLink 405 454 403 454 135 1012 ReadiLink 405 508 414 508 135 1013 ReadiLink 405 537 405 537 URL www bio rad com readilink 47 Meise VivaFix Cell Viability Assay TEA 135 1111 VivaFix 353 442 Cell Viability Assay 135 1115 VivaFix 498 521 Cell Viability Assay 135 1112 VivaFix 410 450 Cell
41. 4 lt 5 ae ae Fa Eo ie Position 11 2 B ZOEM RY ARA e D EV hI YT ZOE
42. Bnter new file nme Enter Enter new file name Enter new file name _MERGE_60_02182015_0102 2 2 B 3 27 28
43. Copyright Copyright 2014 Bio Rad Laboratories Inc ZOE Ver 1 1 Life Science Research web site http www bio rad com
44. 3 ZOE Il 100 240 VAC 50 60 Hz 24 V DC 100W 10 31 C 20 80 MRR DOZA 2 000 m 2 IPX0 ZOE
45. PureBlu Hoechst 33342 Nuclear Staining Dye DAPI 4 6 Diamidino 2 phenylindole dihydrochloride KY Ev AOA Ae DNA Pure Blu Hoechst 33342 Nuclear Staining Dye 56 ug PureBlu Hoechst 33342 1 50ml 1 1 u g ml M 44 1 1 CIPO QE 561 93 350nm 461nm CAS 23491 52 3 95 high performance liquid chromatography DI H O DMSO dimethyl sulfoxide 20 C 20 2 RALEA ICIS PureBlu Hoechst i 20C 1 2 8 C 6
46. Nw YY Settings TAA Edit USB 10 Gallery 10 Edit Edit
47. 135 1303 PureBlu DAPI Nuclear Staining Dye 50 u g x5 AX 135 1304 PureBlu Hoechst 33342 Nuclear Staing Dye 56 u g x A 135 1001 ReadiLink 350 440 KIT 135 1002 ReadiLink 492 516 KIT 135 1003 ReadiLink 555 570 KIT 135 1004 ReadiLink 594 610 KIT 135 1111 VivaFix 353 442 Cell Viability Assay 200 135 1115 VivaFix 353 442 Cell Viability Assay 200 135 1116 VivaFix 353 442 Cell Viability Assay 200 135 1117 VivaFix 353 442 Cell Viability Assay 200 135 1201 CFDA SE 5x100 u g 53 Appendix 2 Ex 355 40nm Bm 433 36nm Ex 480 17nm Em 517 23nm Ex 556 20nm Em 615 61nm Kear aae Fluor 546 568 594 and 610 PureBlu DAPI Labeling Kit Labeling Kit VivaFix 353 442 Cell Viability VivaFix 498 521 Cell Viability esr Texas red Assay Assay EC Labeling Kit a Labeling Kit VivaFix 547 573 Cell Viability Assay VivaFix 583 603 Cell Viability Assay 54 Appendix 3 ue 6 uU m A 0 0 038 848
48. No Automatically export USB image files to USB USB disk ZOE 5 10 30 Sleep 20 ZOE Date and Time Add scale bar to captured image Attach single color images to merge 24 MM dd YYYY
49. eje a 6 ROTI TIIR A B C Z 4 Z 2 Edit 7 Gain LED intensity Contrast Exposure LED ORI E Gain Exposure
50. enter 0 CE ZBE Capure 4 Live 20 2Z 4 Z 2 2 Z Z 720 100 6 2 000 100 12 2 100 100 24 1 900 100 48 2 000 100 96 3 500 200 VOR DIAS 1 800 200 NO 2 500 200 T225 3 200 200 1 200 200 1 100 200 Edit 5 Gain Exposure LED intensity Contrast LED 4
51. Image management USB image management 13 images selected BRIGHTFIELD 2 BLUE 2 GREEN 2 RED 5 MERGE 2 F RED_S2_97132016_9 vf RED_48_02132015 9 7 MERGE 60 0718201 wt MERGE S1 021320 Mins 734 pe 5002 jpg p9 BRIGHFIELD BLUE Channel Option GREEN RED MERGE re pee NIR NN Last 24 hours 24 Last 7 days 7 Last 30 days 30 Last 3 months 3 ALL TAT Reverse selection Export USB LDelete 30 Merge 13
52. CC 4 LED ZOE ao LED 3 3 Settings RPP T ABH We a RF p Y Z ih
53. 100 FIG1 VivaFix VivaFix A B 1 000 000 1 000 000 800 000 800 000 8 8 600 000 600 000 Q 400 000 400 000 200 000 200 000 0 0 100 10 102 103 104 10 10 102 108 104 FL1 area FLT area X 1 VivaFix Jurkat VivaFix498 521 A 3 7 B S3W SSC VivaFix viability Dye 4 50 20C DMSO 1 250 1 20C 48 VivaFixAssay K 1
54. Gallery Select 11 Merge 14C Gallery Select Le Centering ZOE 4 2
55. ex Blue BRIGHTFIELD GRE EN MERGE RED USB Image management ZOE_ALL ex Bluel BRIGHTFIELD GRE EN MERGE RED USB Export all images ZOE_ALL 11 Merge 1 Z 12
56. LED Edit Q1 Q2 Q3 Q4 5 4 LED 4 LED 1 3 FED LED Q1 Q2 Q4 Q3 A ETO 2 LED Q1 Q2 Q3 Q4 LED 2 Gain LED a 3 Contrast
57. E HDMI HDMI TV ZOE E USB USB USB CAM E E ZOE USB A USB USB id paca ela USB
58. 4 LED 5 Apply Cancel et ERR RS HER QE Q Oe CeO 1 2 Quadrants Q1 Q2 Q3 Q4 Gaimd lt 1529 5 Gain 1 152 Exposure time 0 5000 D 0 LED intensity 1 100 O w 75 Contrast 0 100 ZOE 3 IA 8 6 LBD
59. 2 1 0 57 BIO RAD Visit us at http www bio rad com F 140 0002 Tel 03 6361 7000 Fax 03 5463 8480 9 92 94 T 532 0025 Tel 06 6308 6568 Fax 06 6308 3064 1 14 11 812 0013 Tel 092 475 4856 Fax 092 474 5580 2 5 28 Mail life ps_jp bio rad com Tel 03 6404 0331 Fax 03 6404 0334 MNL1450031 M10663 1504B
60. 20 C 5 i l Reaction buffer L 20 20 C a Quench buffer 1 20 xD 20 C 46 50 ug PBS pH 7 2 7 4 PBS 1 mg ml 1 1mg ml PBS 2 1 50 wl Reaction buffer AK 5u L 3 4 ReadiLink ReadiLink labeling dye 1 55 wl 5 60 6
61. CMOS 2 USB 6 USB HE CHT LED 1 LED LED ZOE 20X
62. PureBlu DAPI Nuclear Staining Dye 4 6 Diamidino 2 phenylindole dihydrochloride DAPI 1 NH 4 HCI 0 N NH Hci NH 1 PureBlu DAPI Nuclear Staining Dye DAPI AT rich DNA minor groove DNA DAPI DNA UV 461nm PureBlu DAPI 100nm 2 Gr PureBlu DAPI Nuclear staining Dye D PureBle Hoechst 33342 Nuclear Staining Dye 135 13
63. Merge 2 Position Position 2 Center X Y 0 0 Settings ZA a Center 2 ZOE
64. Wein E eg 70 1 2 3 1 E
65. dd MM YYYY QYYYY MM dd Position Select date format System AN Driver board firmware version 30 Enable Service Mode Firmware update 1 USB USB USB 2 Settings System Firmware update 3 USB
66. Zeiss 490133 E AmScope 50104 MOY ARF yr a Edmund Scientific Co 52105 E Edmund Scientific Co 56926 ZOE 3V 10 www dtsc ca gov hazardouswaste perchlo
67. 1 1 www bio rad com www bio rad com FR Documents
68. 1xPBS 5 1xPBS 6 1x lxPBS 15 7 1xPBS 8 PBS 9 EE 1 URL www bio rad com PureBluDAPI Triton Dow Chemical Company 45 Meise ReadiLink Antibody Labeling Kit 135 1001 135 1010 ReadiLink Antibody Labeling Kit ReadiLink 50 100 up g 2 1 T ez ies Yk eX 1 oh 2 aE TS E ied N E gt iin gt AY tes L ak
69. Staining Dye 56 g x 5 PureBlu Hoechst 33342 Nuclear Staining Dye Hoechst 33342 1 HCI i H C HCI N 1 PureBlu Hoechst 33342 Nuclear Staining Dye Hoechst 33342 AT rich DNA minor groove DNA Hoechst 33342 DNA UV 461nm PureBlu Hoechst 33342 100nm 2 Hoechs 100 gt 80 C K S 60 N 40 S 20 0 0 300 400 500 600 700 Wavelength nm 2 PureBlu Hoechst 33342
70. Viability Assay 135 1116 VivaFix 547 573 Cell Viability Assay 135 1113 VivaFix 408 512 Cell Viability Assay 135 1117 VivaFix 583 603 Cell Viability Assay 135 1114 VivaFix 398 550 Cell Viability Assay 135 1118 VivaFix 649 660 Cell Viability Assay VivaFix cell viability assay VivaFix Cell Viability Assay VivaFix
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72. 04 DAPI To Pure Blu DAPI Nuclear Staining Dye 50ug PureBlu DAPI 1 50ml 1 ug ml 9 6 120 100 80 60 40 Normalized intensity 20 0 l l 300 350 400 450 500 Wavelength nm 2 PureBlu DAPI 550 600 42 1 1 CCN 350 3 359nm 461nm CAS 28718 90 3 95 high performance liquid chromatography DI H O DMSO dimethyl sulfoxide 20 C 20C T 2 PureBlu DAPI jaran gt O KE CENE 20CC 1 2 8 C 6
73. 40 nm 480 17 nm 556 20 nm 433 36 mm 517 23 nm 615 61 nm N A 0 40 W D 7 mm ea EICAT Aa W x H x D LED 20 175 700 4 X Y 6 mm 0 70 mm 12 CMOS 5 JPEG TIFF RAW 16 GB 10 1 1 280 X768 80 180 USB2 0 X2 33 x 30 x 32 cm QK fp E 9 ke ow Hee 63 45 lt 32 em WET
74. D IN NO EVENT SHALL THE COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT INDIRECT INCIDENTAL SPECIAL EXEMPLARY OR CONSEQUENTIAL DAMAGES INCLUDING BUT NOT LIMITED TO PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES LOSS OF USE DATA OR PROFITS OR BUSINESS INTERRUPTION HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY WHETHER IN CONTRACT STRICT LIABILITY OR TORT INCLUDING NEGLIGENCE OR OTHERWISE ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE The views and conclusions contained in the software and documentation are those of the authors and should not be interpreted as representing official policies either expressed or implied of the FreeBSD Project 40 6 6 HEN A Y REAR No i ae 135 1303 PureBlu DAPI Nuclear Staining Dye EL a AK Lo 135 1304 PureBlu Hoechst 33342 Nuclear Staing Dye 135 1001 196 1004 ReadiLink KIT 135 1111 135 1117 VivaFix Cell Viability Assay 200 a 135 1201 CFDA SE 5x100 u g 41 MA 13 PureBlu DAPI Nuclear Staining Dye Hn 135 1303 PureBlu DAPI Nuclear Staining Dye 50ug x5 A
75. EN61010 EN61326 A EMC EN61010 EN61010 PAB AYR
76. ZOE 145 0031J1 AM9 00 PM5 00 JST 03 6404 0331 Fax 03 6404 0334 Email life ps_jp bio rad com Web Web www bio rad com N Trademarks and Legal Notices Android iGoogle Inc Adobe Acrobat Adobe Systems Incorporated ReadiLink AAT Bioquest Inc Triton Dow Chemical Company
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78. ee 2462 1e47 4547560 194 2333 1750 4 081 467 206 2203 1952 3640568 1555 374 1030 1037 778 soi 19 1 259 712 ETT 47 486 314 442 53 Appendix 4 1 1 H TEL TEL
79. en advised of the possibility of such damages 9 Accepting Warranty or Additional Liability While redistributing the Work or Derivative Works thereof You may choose to offer and charge a fee for acceptance of support warranty indemnity or other liability obligations and or rights consistent with this License However in accepting such obligations You may act only on Your own behalf and on Your sole responsibility not on behalf of any other Contributor and only if You agree to indemnify defend and hold each Contributor harmless for any liability incurred by or claims asserted against such Contributor by reason of your accepting any such warranty or additional liability Copyright 2014 Bio Rad Laboratories All rights reserved Redistribution and use in source and binary forms with or without modification are permitted provided that the following conditions are met 1 Redistributions of source code must retain the above copyright notice this list of conditions and the following disclaimer 2 Redistributions in binary form must reproduce the above copyright notice this list of conditions and the following disclaimer in the documentation and or other materials provided with the distribution THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS AS IS AND ANY EXPRESS OR IMPLIED WARRANTIES INCLUDING BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIME
80. ntibody ReadiLink 492 516 Antibody RE Labeling Kit Labeling Kit Assay Assay Labeling Kit ee ee Labeling Kit Oe Assay eee Assay 24 Capture 4 Live Gallery Gallery Gallery Merge 16 GB E JPEG 2500 E TIFF 1 500 BM RAW 800
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82. rate 37 le 5 Software Notices and Terms Open Source Licensing Apache License Version 2 0 January 2004 www apache org licenses TERMS AND CONDITIONS FOR USE REPRODUCTION AND DISTRIBUTION 1 Definitions License shall mean the terms and conditions for use reproduction and distribution as defined by Sections 1 through 9 of this document Licensor shall mean the copyright owner or entity authorized by the copyright owner that is granting the License Legal Entity shall mean the union of the acting entity and all other entities that control are controlled by or are under common control with that entity For the purposes of this definition control means i the power direct or indirect to cause the direction or management of such entity whether by contract or otherwise or ii ownership of fifty percent 50 or more of the outstanding shares or iii beneficial ownership of such entity You or Your shall mean an individual or Legal Entity exercising permissions granted by this License Source form shall mean the preferred form for making modifications including but not limited to software sour
83. s a 39 LAE a S Paa aa T 41 PureBlu DAPI Nuclear Staining Dye ooo icc cceccccececcccccececececcececececeseececeseees 4 PureBlu Hoechst 33342 Nuclear Staining Dye 44 ReadilinkAntibody Labeling Kit ER 46 Vivai Cell Viability Assay MIA ee sca couisnie Ri i 48 CFDA SE Cell Proliferation Assay Kit 50 DO hk ths 52 Apoendic CME OM CO eaa A of ob D3 Append 2 A A aere E eea i 54 eE a I Ee eR i i 55 Append A a a i hii 56 Apondrai a A O AN on V1 ZOE 3 PC
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