EZChrom Elite 簡易取扱説明書
Contents
1. x 1 CasMEcCa 1 Munconiigueed ba 1lTest0d1 lt D gt MicraGiC Checkout Method met e Cal TestDO1 lt D gt dat a 2 SummawHum Oma nceonfigued 1TestOD2 lt Dy MicroGC Checkout Methodmet TesMUU2 lt D gt da 3 Summayhun Dla neanfigued 1lTeslO03 lt D gt MicraGC Checkout Methodmet TestUH3 lt D gt dat Summawhun Dna neonfigued 1TestOD4 lt D gt MicroGC Checkout Method met TesMUU4 lt Ds dal Summayhun ma ncanfigued 1lTeslOO5 lt D gt MicraGC Checkout Methodmet TestUO5 lt D gt dat _ BE SummawHum Dna IlUncaniiuegd 1lTeslU0E lt xD gt MicroGC Checkout ethod met TesMUUE lt D gt da 7 SummawHumn ma Unconfigued 1lTest0dzD 8 SummawEnd imwa lUmconfigured 1lTestOUB lt D gt MicroGC Checkout Method met TesMUUB lt Ds dal 1 Run Status RunType2. Level Sample ID TID Method Filename Agilent3UUU microGC Method MicroGC Checkout Metha Edit View hethod Data hethod Sequence Analysis Contrc 8 3 Data 2 sme File Sequencel Save As Report Template b New Advanced Reports y Oneh i Select Praject Save Frint Setup Print Instrument Configuration Print Audit Trail Instrument Actiwity Log r 6 8 4 2 8 Fi1le Sequence Open Run Sequence Run Seqauence l
3. Data File Type For Acquisition dl 8 3 2 x Sample ID EES 2 Data path a EZChrom Elite Enterprise Projects Default Dat Diata file Kfz 0 4 umher nf unkhnmh Fu In SEHLIETCE FF Rgnetitinng per Fu Create a separate nw in the sequehce Tnr each repetition _ wv lt EW Sample ID ID ID Increment Number Datapath Data file
4. 2 EZChromELlite 2 LChromatography EZChrom AN I Brnadcnm 7 Chromatoeraphy Internet Explorer a EZChrnm Elite RO RE BoundMia 0 fanView 9 7 me ay i 9 Windoms Media Plawer HI HB EE a Thternet Exnlargr 0 Windomis Messenger MEN Ee ED Windows WP YW ld Dutlook Express Roxio Easy Media Creator 7 Basic Edition 9 Windaws Media Plawer WWInHnws Messenger EE EMSWUPIIPE U FE Trfaniew ito es 11 EzChram Elite EZChrgm Elite Release Nates Kg Pr rchiwed Log Wiewer 3 jstallatinn SE H 1 EZChrom Elite 83
5. GC GC 71 C 1TGp chamnela s 0 Bo Sequence Process TCED hanngl S Edit Sequence Analyze co Cut Single Analysis Calibration Copy to clipboard a Preview Run Paste gt Single Run Instrument Setup Seduence Run Peak Group Tables 3 Display Run Queue Integration Events Stop Run i Manual Integration Table N Instrument Wizard Help 72 Peak Width A Manual Baseline Threshold Manual Peak Shoulder Sensitivity W Split Peak Turn Integrator Off Force Peak St
6. 40 OK 5 8 Peak Group Tabale Peak Group Tab1e Peak Grnun Tahles DAD A Marned Fa EE elID ISTD 57317 Hi28ES5G I BE 58722 329351 3 Feak 7514 Minutes 7 51417 D375709 Cut Nitrogen 303HE 1 ne 5 WWIMethane 8 5E 31 4 nd284E Pasta EEN Fill Dewar jmsert Faste jhsert Line Name 3 SS Cut Ranumber Peak IDs Renumber Peak IDs ITD
7. Fe EEE a LL 7 4 Sample ID 1 Sample ID External standard Report hlethod Name EE LC hrom Elite E nterp rlse Frmjer 11 nr dT FM DT 12 OD 14 FM 48 7 2 8 2 Insert Field Sample ID jmsert Fjg Sample ID Insert rahh k lser Rennrting the DS Insert Report k Rin Time Insert Object malyss Time td lame I Extermnal Standard Report Lndate Fields
8. 7 47 11 1 1 Method Properties Method Properties 2 Option Analyze after acquisition OK 4 Fi1le Method Save Hethod Froperties x Description Uptions Calibration Trail alyze durimg acnuistigh every IItE 67 1 1 11 2 1 Single Run gt Single Run Acquisition 2 Print method report Start Single Run Acquisition EE aa E tEZChrom EliteE nterprise Projects D efaultshd
9. Line Number Increment Number Number of unknown runs in sequence Repetitions per run 1 Create a separate row in the sequence for each repetition 9 8 4 3 nEnuErirE Wizard Autosampler Unknown vials of sequence First vial TIncrement by 1 Calibration vials of sequence PFirst vial Tncrement by
10. Velocity Lnlumh mndE Eonstant FrEssurg et point Te Inilil pressure Il 50m Pi 2 E2 Fi tial Ho rb LTmin 3aHH rh ri elocity Initial flow FeEE Ramped Pressure Ramped Flow Column Pressure Flow Program Lnlumm Fressure Frnnram eh ima gE FTFE SLITE Hold Tirg Ru TiTie Fs Fsh TI II En s H OT OT TT H 21 2 3 Signals msn Str rument nt Setup_ Signall Detector Det Range Attn Auto zero Acquisition channel on
11. Print sequence reports Review start 00 KK Memo 8 9 9 9 1 1 Fi1e Sequence Sequence Wizard 2 nt EE IGEnuEncE Wizard Method Met lcts DefaultMethad MicrnGG Checkout Method mat JaITI UIHIEET Sample HinUTit Iriterrial standard amount MFI gh fa
12. HavriEation EZChrom Elite Area Turrant Baseline Check Area T Extermal Standard 2 Exterhal Stahdard same Fractinn Report External Satndard temal Standard o 8 Normalization ESTD Internal Standard ISTD Normalization Norm 45 6 2 Print Report Print EH External Standard External Standard Report Page 1 of1 Method Name EEZChrom Elite E nterp rise Projects Default Method Calibration Practice met Data EEZChrom Elite E nterp rise Projects DefaultDatamulti calihration level 3 4at User Administrator Acquired 11 26 1990 8 49 21 PM Printed 5 28 2007 10 42 42 AM Peak EEE Jo a a a 8 MINM 5 Chamnnel amp Results Pk Name Retenton Fme Area Concentration 12 Peak 5 710 299360 30 000 13 Peak E
13. 18 2 2 3 Agilent6850 1 Temp mment scp Temp Inlet Pressure 0 yalves li Signals YEvent Control a FD HP 7683 VL Baseline Check Trigger Dven parameters Zone temperatures Zone ctual Equilibration time i Maximmum temperature 325 Inlet 20n 01 C Actual temperature 197 29 Detector 1 250 02 C Cryo on Aux 1 2 4 A Rh Ambient Ea Te deleciiof cor 0 Oven Temperature Program Fe Dven Temperature Frogram Oven parameters Oven on Equilibration time Maximum temperature Oven Temperature Program 6 Rate Next Temp Ho1d Time
14. Sampling PP Run time Mie Run Time Acquisition delay Trigger 0 22 2 FID Heater H2 flow Airflow Makeup flow Constant column makeup
15. 30 3 4 31 3 5 1 32 3 6 Run Queue 32 4 33 NO jc 33 4 2 34 Peak Width 34 Threshold oe 35 3 36 4 Analyze 36 5 37 Area 38 5 39 5 1 39 5 2 42 3 43 4 43 5 44 6 45 6 2 45 6 2 45 7 EETRTRNTTNTNNN 47 1 47
16. CooldownWMicroGC met 5 Contro1 Down1oad Method GC CooldownMicroGC met Agilent 6850 1 IMetlodl Instrument Setup 2 Temp 0ven on Inlet Detector 3 FID TCD Makeup flow 6 File Method Save As Cooldown6850GC met 4 Contro1 HP6850 Down1oad GC Method GC Cooldown6850GC met
17. Repetitions per level 1 Create a separate row in the sequence for each repetition Clear all calibration at start of sequence Multiple calibration sets 2 Number of unknown runs between sets 94 0 8 5 HEqUENCE Wizard Reports GLTITIaF Ip Imcludg unknnwun Fuhs In suimmary report Re jmclude calibratinhh runS in sumimary Feport Swstem Suitability Run calibrat
18. 3 AgilEnt3HHU micrnGaG Hethod MicroGt Checkout Method met Data Hone Project Default 2 File Edit Wiem hethod Data Sequehnce Ahnalys Dontrol Reports Windomw Help 1Tcp chamela lt 3 2 Agilent 3000 GC 1 Control Down1oad Method Eilent3OO00 microtst Ethnd iErnG CheErknut ethod met Data Nnne i File Edit ismm hethod Cata Sequence malysis Control Reports Windom Help lt lt 1 TCD Chanmnela 0 Preyiew Run pp Single Run GrFShift F9 Sequence Run ChShiH FB Gtnh Fn Fin uue 1 Navigatinn nntrnl Instrument Setup Fi uug a Instrument Status Externd Rn Downad Method 28 2 Contro1 Nayigation RK VS ei 0 Cinhtrnl Instrument Status ia mstrument Getup EC tetta Instrumernt Status Instrument Status
19. Run Sequence RnSequence sgHHefiCg nformmation 6 LEHCE Mame ProiectstDefaultSequencetMicrgF TagstGEnuEnce se La Hum rahge Help EEHi Bange Frinhinn FPrint method reports FE bas EET EE ri I Print sequence reports a GalIHTalinfgE Eegin ru las Sequence name Frocessihg mode arma lt Eracketing nne Runrange Al11 Range 4 9 4 9 4 4 Printing Print method reports
20. 6 Sequence uF _Hun Statusl RunTwpe Level oOEDR ODNPSIRE 12Heo 52DG1D VBD2H HRYS2 unknomn na Unconiigued 1 PNASTDS MicroGCCheckoul Melhod mel calbraian level 1 dal 2 Unknownm na Unconigued 1 PNASTDS MicroGC Checkout Method methulti calbration level 2 dat 4 Unknown nta Unconfigued 1IPNASTDS MicroGC Checkout Method methulti calbraion level 3 dat 9 Unknown Ia Uncofigured 1IPNASTDS MicroGCCheckout Method methulti calbraion level 4 dat a nknawn hn a Uncanliured 1 FN amp STDS Microi Checkout ethod methulti calibration level 5 dat EE a nknawn na Unconfigured 1 add MicrnhL Checkout klethod metnulli calibration lewvel b dat Leve1 Run Type Clear A11 Calibration Eile Sequence Save As 61 9 9 2 1 Sequence Process
21. Run Time Zone temperatures Zone Set point Actua1 19 2 2 Inlet Pressure Cr amp Temp Inlet Pressure Oi IT DY Event Control FID HP 7683 VV Baseline Check 3 Pressure unit Fs Capillary column Length m Total flow 25 34 ml min Inside diameter 10 32 Trigger Split Splitless inlet Film thickness 10 25 Split made Splitless Fulsed pressure 1 45 PFSI until mn Column made Canstant Pressure Purge flow len mL min D75 min f SH HoW 1 mL min Snltratie 1 5 Set point ctual Initial pressure 25 00 FSI 25 52 PSI Inilial lawi 176 mL min 3 00 mL min mL min 0 Welocity Cm Sec Has sayer Column Fressure yen Time gt Pressure unit PSI kPa Bar Use this inlet Total flow Split Splitless inlet Splitmode
22. 10 1 1 Method Advanced Advanced Method Options Advanced EM Advanced Method Options DAD Export ala Esnnrl graphics Enport Custom Parameters Calumn Performance FL Export Enabled Expart Enabled Esport Ihese parameters i ISTD cprirahor Peaks PF atop Time a 4 1 Field separator lt Tabs a a Palh fnr enport files IE iEZChrom EltetDatatEsparI2HH5H524 3 3 Standard Reports 1 1 5 Parameters to export
23. pda estd unknown O02002 dat lt EB gt gt UNK0n1 dat UNK0n2dat UNKn3dat UNKn4dat Vac5H2 dat 4 Modified any time Files lt any time 2 Find Now New 5earch add Delele 60 9 0 unEnuErnre Wizard Select Files Data files E BzChrnrm glitE EnternrIsE hrnjEcts HeTaut Hata muti calibration lewe BzChrnrTi elite enterprise projects detault data multi calibration lewe E BzChFnITi ElltEEhter DF EE DFE detau tdata multi calibration lewe BZChFnITi lte EtETF ISE FnlEcts HeTault Hata multi calibration lewe E BzChFnITi glitE EhterhFISE hnlECts HeTault Hata mi calhratinn lewe E BzChFniTm glitE EnternrIsE hrnjBcts HeTaut Hata Tui calihratinn lewe 0 mm
24. View Header Footer Efermnal rn nr Reprort Fage l El oy a gt We Na EE TC ram E lteiE mie ri Firm ect et ul wihar ra Ha n Frar rm mami at i Hie Fie OP re ee the Tt mnt nl tim lee ni meire Ir2OrlWy Bi 1 Printed SHOT Hi1T AM 1 o FEE CC Show Data at Design 1 Time STrareTata Headers Pf MH ET Tie Lielete l Riahnrt Prnnerties 4 7 7 2 ra hz lt BZ UU se 7 3
25. Export these parameters 6 Path for export files 7 Advanced Method Options 8 File Method Save 65 10 9 Analyzel 10 Excel Excel 10 2 Time Sample IH File Name Method Nailser Name ial 48 53 PM PNA STDS E EZChror E EZChror Administrat HA 8 49 09 PM PNA STDS E EZChror E EZChror Administrat N A 8 49 21 PM PNA STDS E EZChror E EZChror Administrat N A 8 49 34 PM PNA STDS E EZChror E EZChror Administrat N A 8 51 56 PMPN STDS E EZChror E EZChror Administrat N A
26. Width EE g Time bB 15157 hinutes npltude H HLH11H hks Channel 5 DDS FH HUS nl Width 3 Inshruchnrs FI Select Paak Starting Point ts Hie HH FL HI Fl Ctart Time 51 Minulgs Inn Timme 6 765 iiates Walue lp 11 HH awent tn ahnd data Cadd ewent to this data File only halae ow 94 Threshold 1 Threshold 2 er x Time 3 hinutes Mnpltude B e ODS hlts Channel 5 EH ST _ Threshold nstructions Sat huWhuta 3 Threshold Add to Table Threshnld I x Gtart Time ee Minulgs Inn Timme 7 lirates Wale HH event tn Method data add ewent to this data file only halyae Now
27. multi calibration lewel 4 dat 5ysSuith2 dat multi calibratia lewel 5 dat BysSuitU3 dat multi calibration lewel Hat SysSuitHH4dat pda estd lewel 1 5ysSuitHH5dat SwsiSuitHH dat pda estd lewel 3 UNEDOT dat pda estd lewel 4 LNKI2 dat pda estd unkrowrn HH1nn1dat UNKOn3dat da estd umknawn HH202dat UNKODA dat Unc5H2 dat rmutti calibration lewel 1 dat 2 alFiese 8 0 0 Firnd files that match these criteria Sample ID Created anytme Find Mow Modified lawime 7 New Search ptinns Method Results IMos Riecert I Searchresults UZ CC ACC RV PT FE AN OE Options Method Current From Results Original1 Acquisition Results 9O 4 4 2 Peak Width LL
28. 69 1 2 EZChrom Elite 1 2 2 EZChrom Elite 1 File Exit fm Agilent3000 microGC Method MicroGC Checkout Method met Data multi calibration leyel 2 dat Project Default File Edit View Method Data Sequence Analysis Control REparts Window Help Method AvIX gt m Data H Sequence Channel A Time 8 84356 Winutes Amplitude 4 6e 005 blts Report Template hdvanced Reports Select Project Print Setup Print Instrument Configuration Instrument Activity Log Recent Method Files Recent Data Files Recent Sequence Files 2 BZChrom Elite ny EZChrom Elite Client 5 3 File Exit EzChrom Elite Client Server File Edit yiew Taols Help pe dbJE WIDE lk ON PrVeroup is WRAWASAK Drise Rename BAKI Big ACE1 EEE AeilentGC Properties microGO 6B
29. 4 3 1 Integration Events i x Event StartTime StopTime Waue et 4 2 Theshod 738 1 3 wih E14 V V G8lBl 0G521z ti arsa Bs Backwuard Horzontal Baseline Lowest Fnint Hnriznntal Easeline Targent Skim Front Tarngent Skim MI Menaliwe Peak A 2 Event 3 Start Time Stop Time Value 4 4 Analyze Analysis Analyze RE 8 a EZChrom Elite ser drnirstrator Logged 5421 42007 2 00 07 Ph Audit Trail eT tat ED 3 Tupe width Start Minutes Stop 0
30. Apply To A11 OK 56 4 5 1 Reports Mayigatinr 1 SE Tramssad Rahnrt trea ET Lurreht Baseline Check Exterhnal Standard External Standard samel T Fraction Report TInterhnal Gtandard Tnrmalizatinn Fage 1 nf1 Aren Report Data File E EZChra EliteEnterprise ProjectsD efault D atauwulti calibration level 4 dat Method E EZChrom Elite E nterprise ProjectsD efault ulethodC alibration Practicel met Acquired 11126A19908 49 34 PM Printed S30ARO07 12 41 40 PM Retention Time Mies Channel amp Results Retention Time Area rea Height Height 5 719 459101 25 60 54388 29 97 6 558 389384 21 72 40982 22 58 8 264 363614 20 39 31227 17 21 8 330 278975 32 29 4893 30 23 Totals 1793 74 100 00 181490 100 00 3 Print Area 3 4 Area Fape l of 1 ra 0 Report Dats File EUChrnm ElitetEnmterprime Freeets Lefont Cataymubti calibration lewel 4 dat gh EUChrnm ElitevEmlernri MPTTjEctsiLefaultlethnrfMCamlmhrahinm Pra
31. Sp1it Splitless Pulsed Split Pulsed Splitless saver I Capillary column Length Inside diameter Film thickness Gas 20 2 Actua1 Column mode Constant Flow Constant Pressure Initia1 pressure Tnitia1 flow
32. oo 48 3 48 4 Sample ID 48 5 49 50 8 LL 51 8 1 51 8 2 II 56 3 56 4 57 9 59 9 1 59 9 2 0 oo 62 10 65 10 1 II 65 10 2 66 1 1 67 11 1 4O 2 67 11 2 68 1 1 3 68 1 2 EZChrom Elite 69 2 KK K OK K RE
33. Show at instrument startup Instrument Wizard 5 AeilentGC 6850 Method multileyel calibration met Data multi calibration leyel 3 dat File Edit Wiew Method Data Sequence Analysis Control Reports Window Help ES T1 Detector1 Navigation x Channel An j x Time 6 81928 Mnutes Amplitude 0 00015 vblts A nstrument Setup mm comm Integration Eyents PN ESTDS Peaks Groups Z Reyiew Calibration Adyanced nnD Custam Report EX System Suitability Data MManual Inteegration Fixes Tiled Display Integration Event Peaks Graups Table A System Suitability A Manual Integratian In nnn
34. agilent 3UUU Micro GC Instrument Status Fieady Channel Channel B Temperature deg C Temperature deg C Setpoint Current Setpoint Current Sample inlet 4D Sampleinlet 4D lll Inlector Inlector 34 Columr 9 Columr 34 Column press LU 47 psi Columnpress DOO TCD Filament OF Dif TCD Filament OF En Agilent 6850 Contro1 HP6850 Download GC Method 585bU GC Method KS 685Ua_met Data Instrument 10002 Preyiew_dat Project Default File Edit View Method Data Sequence Analysis Control Reparts Window Help 1 FD CM GQ Preview Run cdc ae p Single Run GtrhShift F9 Cantral nstrument get Sequehce Run Gtr Shift FB ratrument satup Temp Stee Ru Eyent Cantl FID HP 768 Run Gueue RE Instrument Status Signal 1 YAcquisition channel on Detector Frequency 1 5amplina Show Autosampler Tray Hange Baseline Check Set GC Defaults Farce Host Ready GC _ Keyboard Locked Auto zero 6850 TInstrument Setup 29 3 3 3
35. 1 Control Preview Run l Preview Rnn mo MR Time 7 8d661 Minutes mplitude pA a 9 dd Trace 2 dd tinle Traces DB ee Axis Setup ppearahce Full Lnznnm lgar weraws nratInhg k Ltilities k raphical Frogramiming k Froperties 3 50 3 3 4 SIngle Run Acgquisition Fiun infnrmahinm Flbrats DE kp Increment Numher start Single Run P Single Run Acquisition Llsgr Mame Methnd Mame jmstrumeht Name L
36. 1 Autosampler injection volume 4 alhratinn HL Et 4 alihratinn path fe EZChrom Elite Enterprise Projects Default Dat Calibration file Galo gt dat Hiumber of calibratinn lewesE Repetitions per ewel 1 ly Clgar al calibration at start of sequehnce Crgate a separate Fn in the sequehnce for each repetition hultiple calihratinn sets Tibet ka he betmeeh et Fetersperse calibratinn wals mith Hm wials Reise calibratinn wials trom irgtcaliHratinr set Calibration ID TID Calibration path Calibrationfile Cal Number of calibration levels
37. 22AI IA 69 Agilent 3000 GC 69 Agilent 6850 69 12 2 EZChrom Elite 70 A Agilent 3000A GC 71 B Agilent 6850 1 C 72 72 i 1 4 4 4 73 74 ll WT 74 Agilent EZChrom Elite Agilent 3000 GC Agilent6850 1 EZChrom Elite 1 EZChrom Elite 1 PC Windows Windows
38. EZChrom Elite Client Seryer File Edit Wiew Tools Help Current location group is KAWASAK w Z The Enterprise wesak i ACET eilent3OOD Sod2Dx microGO IL EZChrom Elite Lnggim gr hame administrator Passnmnd my Frnjlgc hgfaull Login User name Password Project 12 1 EZChrom Elite 4 Imstrument Wizard AgilentGC 6850 x EEE OK ee SE Hiun a sequence of samples 2 Py ia
39. MO 17 2 4 Trigger Type EZChrom Elite nx Agilent 3000 Micrm BE ut Trigger ne am starts Imaiatglu after clicking ohn 5tar SuEhCE C LISI HS do nt pase betveern IE anu neratnr has to press nter to start the ruin Sequehee CHUISIHI PTE LSE For nirTmahinh hhuBEh IM Esterhal IF the data sampling 1s started Irnm an enternal Iri ner select this option The tupe of frigger is HEsl ae In IIrument configuratiorn Select mheh Using a corntact clngur from an autosampler or manial inector None Start Manual BEnter External 5 TInstrument Setup
40. Properties 9 Fit Type 1 Point to Point armed Faks raups a Cab Fs do 1 WW lReees Fepsce glPaimLta Pint Replace Nitrogen Foint to Point Mglhiarie Point to Point Zero 41 5 10 Level 1 Leve1 2 Leve1 3 11 File Method Saye 5 2 1 2 Single Analysis Calib
41. Filename Edit k 3 Drawi 0 Method Name EEZChrom Elite Enterpri Liata Description Le 3 44 a Znm Data EMEZChrom Elite Enterpri argin Setup Injection Wolume User Administator Frint Prewiem Sample mount cq uired 11 GE LPM Ft ISTD mount Printed DT 13 HH 1 FMT Tntal Factar 6 ME Mult Factor 1 SU Mult Factnr 2 gt Ruler 2 Sample ID MSDS ID 7 5 Print preview 2 File Method Save 49 7 External standard Repaort Eth Mme FE VE Chrmm EliteEnterprise Pro jects De fault Method alibraiinmn Fracitice met FTEH EEE hrmm ErtEmiErprisrPrnjEctsilefaulHhataumulti rmihratirmm Erl 4 dat Lser mimisitrator TillllmurH TE ND dd FM Frimted NT DT Wi Sample ID PNA STDS F iBm Timg Ph EEE LGA al mm
42. Salert Project awe ns Detault Frint Setup Frint Print Imistrumient Configuration Import jhstrument Setup Save Method Hs Tnstrurmernt ctiwity Lng k mudit Trail Recent hethod Files k 2 Saye Method File As dE EE Method gt EBIFIDChgcknut mgt 3 1 glght11HL ab Test hethod met Calibratiam Fractice met ultilewel calihratinn imgt H pda Bt mat MicrnGC Checkout hethod j Diescription kgthnd created Fnr column performance check met File Method Save 9 2 3 3 3 1 1 File Method Open 0pen Method 2
43. EY 0 Pi EC eho 6 Sequence Process 4 GEENEE Inforrmation LBHCE hame FroiectstDefaulk5equenceVMicroG TstGE ETCE g Laricl Help Frocessihg mode Reintegrate lt Eracketing nne EEHi Ti Hanng Print method reports Hesulks rewiew pause after ach uh ahrahinh rewien Piinlsequence reports pause after each calibratior set Sequencename Process1ng mode Reintegrate Use last results Use original results Review only Review i Results review pause aft
44. Filament Actua1 24 6 2 HP 7683 Injector Sample washes Sample pumps ViscosityDelay Solvent A B post wash Solvent A B pre wash Pre Injection dwell Post Injection dwell Sampling depth 3 6mm 2 0 30 0
45. 12 DO DD a dd E EZChrorE EZChror Administrat na 1 Report hannel Peaks Latm Tirne Sample IH File iarme Areas Channel 11 11 26 1990 B4853 PMlPa sTDs E EZChrar IPkng Ret Time Area Ara Hight Height Flags 1 QB5B 5 39 188 0 726 Bw 2 D996 1155 47 1 Q661 vVw 3 1 164 643 Q 261 43 Q166IVw 4 1 44 340 Q138 73 0 282 VH 5 3 819 267 108 53 Q 205 5 738 63056 25 61 1 7790 30 09 BE 7 5 956 Q Q B 6 5B7 5250B 21 327 5631 21 7511BB 9 51 4 437 177 5 0 232 1 V 10 B 303 47432 19 265 4213 16 273 Wn 11 B568 79411 32 253 7667 2815 V 15 15 ITatals 246209 100 25BB9 100 Standard Reports Area Report 66 Autosample Feak AAA Peak BBB Peak CCC Peak DDD Peak EEE Mane Mone Mane Mane Mane Mane 563I56 170015 299350 462656 779320 41161 40 891 52508 437 141753 166r 249693 2052 53Gll 5667324 8964 17777 13132 47432 1345637 239475 375582 53a854 697 1 1 11
46. Agilent 3000 GC Agilent 6850 EZChrom Elite Agilent Technologies E Chrom Elite Agilent 3000 GC Agilent 6850 E Chrom Elite 2007 7 EE 5 EO a 9 1 EZChrom Elite 11 2 15 2 1 15 2 2 Agilent 3000 GC 16 1 GC 16 2 TCD ChannelA 17 3 17 4 Trigger 18 2 3 Agilent6850 19 1 Temp 19 2 Inlet Pressure 20 3 Signals 22 4 FID 23 5 TCD 24 6 HP 7683 25 7 Trigger 26 2 4 27 3 28 3 1 28 3 2 28 Agilent 3000 GC 28 Agilent 6850 29 3 3
47. Method Data File Type From existing data files 99 9 3 4 PS EP i a Sequence Wizard Select Files Data files Open Data File multi calibration level 1 dat multi calibratinn lewel 2dat multi calibration level 3dat Imulti calibratinn lewel 4 dat multi calibratinn level 5dat multi calibratinn lewel 6dat CH Find files that match these criterla QCnn1 dat QCnn2dat STDnn1 dat STDnn2dat SysSuit H1 dat SysSuitn02 dat SysSuitn03 dat SysSuitn04 dat SysSuitU05dat Sample ID Created nalyst Add
48. er alcalibaton Submit Priority Cancel a alibraton jarleyl Help Fun informatior Sample ID F317 7savents Method ENEZChram Elite E nterprisesProjects Default M Data path ENEZChrom Elite E nterprisesProjects Default D Data file zno70531_splid1 lt 003 gt Numberofreps I2 TT Piint method report Amount yalues Sample amount 1 Baseline Check Internal standard amount Mulliplication factors 1 1 1 Begin run Dilution factors immediatey FiihEpliBraligrrEnpil hreplieates EranErerlicates utosampler ial 1 Injeclian yolume 1 32 4 1 File Data O0pen Open Data pen Data File Data A wm multi calibration lewel 1 dat STDODT dat multi calibration lewel 2 dat STDOD2 dat 0 0075 multi calibratinm lewel 3 dat BysSuitH 1 dat nnnsn
49. hl A Resnlis Mae Retenmion Tinie re 13 Pe 5 719 5 Peak BEE la Peak CCC 1 Dad DDDD 5 ET IT Peak EEE ET 5 Pa FFF 5 3 1 U 8 8 1 i File Sequence Sequence Wizard RE eilent3000 micrna thn ro Checkout ethc Edit ghnd jm ghnd Data Seduehcg ha ys 5nntrr Ha Data k Bequence Sequence Wizard Feport Template Newm dwanced Reports ne Senuence Wizard Method lt Mgthn ects Default hMethod MicroGo Checknut Method met hata Fi Type Frnm gxisting data files Lnt walues ample amoaunt Irterral standard amount 1 hultiplication tactors Dilution factors vt lt a Method
50. sd aaa ria sect cami a ham FF cenwe A Views Far Help press F1 EZChrom Elite 13 1 EZChrom Elite KK Memo 14 2 2 2 1 1 File Method New heilent3O000 microtGt Methnd untitled met Data hone Edit Wiem Methad ata Sequence Analysis Control Rennrts IMethod 3 Methad Wizard Pata Mew LIEH nen Report Template aw Hiwance REhnrts B elect Froject 5aw ns Detault Frint Setup Frint Frimt jmstrument nfjguratinn mnart Imstrument Getup Imstrument ctiyity Lug udit Trail Fiecernt hethod Files Recernt ata Files Riecent Sequehnce Files Exit 2 aeilent3000 mcroGC
51. The current method has chaneed Wauld nu like to sawe It Instrument Setup 15 2 Instrument Setup Aglent 3000 Micro GC NL Trigger harnel Lnlumn ag 1Hm w 15hum w 2 Hum Inlgchinn Inlgct hmg SE Fasl ru kime 2 SE aample pump OF Continuoys tTimed In Temperatures cnnlrnl ample inlet I Hr 5 Hgn n ls degC Kon finn degc we Fiessure cohtrol nector Lnlumm Eullhralinh IIme 1 5 Enlai NOn Fost rr II ps 2 2 Agilent 3000 GC GC Inject time Post run time Samplepump 10
52. Slow plunger 25 2 7 Trigger Type EZChrom Elite LL Instrument Setup None Start Manual BEnter External 26 2 2 4 1 Filel Method Save Asl AgilEnt3HUH micrnG Method untitled met Data Hone File Edit Wiem Method Data Sequehce Analyss Control Repr Methad Wieard FORUN ET Data 3 New Gequence k Open O Report Templateg Fk dwahnced Fieports k
53. le Calihratiarr rtE Chrom ElitetEnterprisetPraiectstDefaultND IEaricalibrafign faT leygl Fit Clibratiori re EaT replicates rae Tenicates 11 3 1 Sequence Run Run Sequence l 2 Printing Print method reports Start Run Senuence BL sn BB ae ProiectsLefaultSequencehdicrobGL TestSequenced seq al 5 EBON 68 12 EZChrom Elite 12 EZChrom Elite 12 1 Agilent 3000 GC 1 IMethod Instrument Setup 2 GC Sample Inlet Injector Column 3 TCD Channe1A Filament 4 File Method Savye As
54. 5h System Activity Log Yr Exit TN EZChrom Elite Client Server EZChrnm Elite liBnt 5iErwe OK tn Exit EZChrom Elite Client Saryer ex GOY 70 A Agilent 3000A GC GC 550 690kPa 5 5 6 9kgf cm LAN GC B Agilent 6850 GC LAN
55. BE 5 986 g91 30 000 14 Feak CCC 5 558 24959 3 60 UUOU 16 Feak DDD 45 2032 G0000 17 Peak EEE 8 264 239473 G0000 18 FeakFFF 8 530 376822 G0000 Totals 1168293 300 00 46 7 7 EZChromElite EZChrom Elite 7 1 1 Fi1e Data 0pen 2 Pile Report Template Open External Standard Srp EZChrom Elite Area Srp Area Externa Satndard Srp ESTD Interna1 Standard Srp ISTD Normalization Srp Norm 3
56. EH Reyiew Peak Calibratinn DAD A _ RF LastArea Hesidual Hep On15858819056d771 nd117636678116549 DO100213789417424 EE 6O 15732454545e 005 D 73320 584971513527e 005 n External Standard urye yerane HF OD 000105411 RF SIDey 3 55858e I5 RF HSD 33 7591 Scalina None LS Weighting Nane Farce Through Zero Hf Heplicate Mode Heplace Point to Point Fit 3 File Method Save 44 6 6 1 1 File Method O0pen l 2 File Data O0pen 3 Analyzel 1 Reports Report View
57. Flame on FID Electrometer on FID Actual 23 2 5 TCD Instrument Setup Heater Ref flow Makeup flow Constant column makeup Negativepolarity
58. Temperature contro On A Sample inlet Column ES Injector 100C Column 60 120 C Pressure contro Equilibration time EPC Column Post run 16 20psi 30psi 2 2 TCD ChannelA Imstrument Setup Aglent 3000 Micra GC RL Tnaggr GE TCD Channela we EOL charnnel on ly Filamehnt or ll r Fimnuemc Hz ME Perind noa les Easeline offset n mW Suitable for min neak width at base
59. arcgl Help hethod E MEZChrom ElitetEnternrisetPrajectstDefaulttk Data palh EEhrom EliteEnterprse Proects Default Ll aa fle Test Sample IDs Mumher of reps Print method report Mon yalugs ample amourt Interhal standard amnuh 1 ualtiplication Factors Begir ru hillnm Factors immediately G TEIiEatEg ElaE TEHlitEg Sample ID ID MM ID Method Data path Datafile Number of reps Start FID 6 24 12 40 Minutes T
60. art Valley to Valley Force Peak Stop Eas Horizontal Baseline Move PBaseline TT T1 Reset Baseline Backward Horizontal Baseline Lowest Point Horizontal Baseline Reset Baseline at Next Valley Tangent Skimming M Adjust RT Window Front Tangent Skimm1ing 7 Adjust Group Range gg Min1imum Area Define Single Peak Ww Negative Peak Define Peaks WS Disable Peak End Detection 7 Define Groups 5 Hz PT Reassign Peak SuggeSt Sampling Frequency 13 Review Save Method As 4 IMlemo 3 Agllent Technologies
61. cticel met Mequired TT dA PM HMDUTT IT 4T 47T PM Fg to Timg Chanmel A elts Rabigpmition Time 39HI ET ET Eirmil FI EE 8 5 5 Peak Group Table 5 1 1 Fi1e Method Open 2 File Data Open 3 Analyze 4 Define Peaks m m mege AI Granhlcally efine Faaks Define Feaks 5 Time Wntag rnp
62. er each run yi Calibration review pause after each calibration set 57 62 9 2 Start l Sequence MicroGC TestSequence4 seq CampletelCA amp L SMB CCA 1 Unconfigured 1IPN amp STDS Microi Checkout Method met calibration level 1 dat 12 Unkmowmn Dna Unconfigued 1IPNASTDS MicroGC Checkout Method methulti calibration level 2 dat 3a Unconfigured PNA STDS ulti calibration leye 3 da Unconfigured PNA amp STDS ulti calibration level 4 da Unconfigured FNA STDS ulti calibralion level 5 da Unconfigured ladd MicroGC Checkout Method met ulli calibration lewel bE da MEE EE I ma ma 5 Unknown ma ma 63 9 KK Memo 64 1 0 10 Excel
63. ime 2 67437 Winutes Amplitude 11 453 pa J1 Contro1 Extend Run l me 0 Extend Run bb Single Run cwhshiftFg O0K Sequence Run GtrtShifttFB ny Stop Rur Extend Run EE Run ueule Estehd run by 5 HH Minutes Earncel nnitnr HG Help Windom Help HFEH5II 3 ho utosampler Tray Easeline Check 3 6 Run Queue 3 Display Run Queue I tatus Beein Processihg lmmediately i Run E Chrnmi Elite Enternrisg FFnjcts LiTa Delete hy Runs Lia lste HEE Ei i Frint Mgthnd Rearts 3 CX Single Run RFit iiEnulElirglREnnft5 Sequence Ruh GulfiCB PFnCEsE Single Run Acquisition 3 Calibrate Eg atator eye 1 A
64. inn as system suitability First calihrati HsEt ni 1 alibratiarn szEt LC Check Standard after ewery unknnwris set QU check standard Ihclude method contents report lt 2Xx gt Summary Include unknown runs in summary report Include calibration runs in summary report System Suitability QC Check Standard QC Include method contents report 2 NE 8 2
65. ltude HHHH1H htts Channel m4 H IH8 Ln ng hefime Peaks Instruchinns Llick the beginring of the range ot En heaks nnu warnt to define 1 Ca nnmd EE 9 OH i HI HHY I Ih I IH 39 5 6 Define neaks In rahge 527 inules 3 inutes Gtart hn Inh Hg Fietentior hm wdon Relatiwe bsnlute 25 dg ILIIE nls uantitate neaks or Area lt n WW mdd lneaks tn table inimum peak ra Hehlace existing peaks in table Add all peaks to table Replace existing peaks in table sn IE Hglp Define Peaks Define peaks in range Retention time window Units Quantitate peaks on Area Height J
66. of OD Im ghslhwltu Ghanard Hun tim DN mir sItinhn dla In nl Filament on On J Autozero Acquisition channel on Sensitivity Standard High Sampling Run time Acquisition delay Trigger 0 3
67. ration Analysis Analysis Single level Calibration a TLe1IIIII TeLE Hethod MicrotGt Uheckout Hethod met Data multi calibration lrel File Edit Wiem hethod Data Sequence a 5 1 TD Chanmel ns E r EE malys 7 Simgle Lawel Calibration nals ontrol Beports Wimdamu kl halyze tr hift F1 Hgslp 3 Calibrate Calibration level 1 1 Clear all calibrationl Analysis ingle Lerel Galibration halusis InF rmatinn Sample ID IPNa sTD5 Melhad EMEZChram EletEnterprsetFroiecuDelaulyMe Cg Data path EMEZChrom ElitetEnterprisetPraiectstDefaulNDs Data fle muicaibaionley1dt Print melhnd repnrt ww Calbrate hratinh lewel Flgar calihralinn for ayl Print caliBralinr report Flgar replicatgs Mournt wales verade replicates Samples amount Internl standard amourt ilutinn factors hd ral
68. tiplicatiorn Factors 1 4 Start 42 5 O1 OO Single Run gt Calibratel Calibration level 1 1 Clear all calibration GimglE Rum cquisitimn Huh inFarrnatinh wi Ealibrate ap 2 ahrahinn lay dethod EMEZChrom EltetEnterprisetProiectstDefaulttM Elear all calibration Cancel Data path e EZChrom ElitetEnterprisetProjeclstDefaultD Clear calibration Far lewel Help Data file Caiibratian Sampls lt Ih gt Print calibratian report Number of reps hh Print method report Clearreplicates werade replicates mnunt yalLls Sample amo
69. unt nternal standard amount Ped altiplic atinn Factors 1 Begin run Liilutinn Factors 1 limmediately Descriptior 3 Start Method Properties Option Analyze after acquisition 67 5 4 5 2 5 3 2 Calibration 1evel 2 C1ear a11 calibration 8 51 43 5 5 5 1 Review Calibration l Review Peak Calibration 2
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