LAMP 法用プライマーセット L. londiniensis 取扱説明書
Contents
1. 1 LAMP DNA Positive Control Llon LAMP lt2. LAMP Positive Control Llon Positive Control Llon LAMP Positive Control Llon
3. eg7o e 5 londiniensis LAMP Loop mediated Isothermal Amplification LAMP DNA DNA LAMP Eiken GENOME SITE http Ioopamp eiken cojp amp
4. 1 1 1
5. TEL 076 451 6548 TEL 03 6712 3885 FAX 076 451 6547 FAX 03 6712 3886 E mail suppot nippongene analysis com E mail feast egenome csJjp fujitsu com URL http nippongene analysis com URL http genome e mDJD Copyright 2015 NIPPON GENE CO LTD All Rights Reserved
6. 5 LAMP Positive Control Llon 6 LAMP LAMP Legionella londiniensis 7 LAMP ondiniensis e Genome Order e Genome Order http genome e mpjp I LAMP LZ ondiniensis
7. 1 5 mL 0 2 mL Loopamp IV Positive Control Llon Positive Control Llon 5 0 pL 25 0 uL 1 DNA 65 C 60 LAMP
8. Positive Control Llon Legionella londiniensis DNA 1 Loopamp DNA Loopamp 0 5 10 uL 10 100 uL 100 1 000 uL TE Buffer pH 8 0 0 2 mL 1 5 mL 2 0 mL LAMP
9. sr DNA Polymerase 80C 5 95C 2 3 3 1 3 2 Tt Legionella londiniensis 4 10CFU 100 CFU
10. V A 1 B A B VI
11. 20 0 uL Loopamp Distilled Water 5 0 LL 1 2 5 0 uL Positive Control Llon 5 0 LL Loopamp LAMP 65C 60 2 5
12. 2015 5 LAMP ondiniensis NE2011 LAMP ondiniensis 1 LAMP Legrone lla londiniensis 2 20C 6 3 4
13. gt i 10000 ppm 1 ii iv 70 1 1 1 TE Buffer TE Buffer 100 CFU 5 uL 10 CFU 5 uL 1 2 100 pL 0 2 mL 95C 5 5 2 2 1 2 2 2 3
14. 9 1 2008 Legionella londiniensis 20 65 2 Notomi T Okayama H Masubuchi_H Yonekawa T Watanabe K Amino N Hase T 2000 Loop mediated isothermal amplification of DNA c e c Acids Res 28 12 e63 3 Prince AM Andrus L 1992 PCR how to kill unwanted DNA Biotechnigues 12 3 358 2015 5 Loopamp NIPPON GENE
15. amp 1SO 13485 2003 http www nippongenematerial com II LAMP L ondiniensis 48 NE2011 Primer Mix Llon PM Llon 125uL 20C Positive Control Llon PC Llon 100 uL 20C 1 20C 6 20 C
16. Loopamp DNA Legione la londiniensis LAMP LAMP DNA Legjone lla londiniensis Legione lla londiniensis LAMP LAMP DNA eg7o e 8 ondiniensis Legionella londiniensis
17. 2 4 2 7 Primer Mix Llon Positive Control Llon Loopamp DNA 2x Reaction Mix Distilled Water sz DNA Polymerase 20 C 1 x 3 1 5 mL 2 0 mL 1 x 3 1 8 1 2x Reaction Mix 12 5 uL 112 5 pL Primer Mix Llon 2 5 uL 22 5 uL Bst DNA Polymerase 1 0 uL 9 0 uL Distilled Water 4 0 pL 36 0 pL 20 0 pL 180 0 pL Loopamp
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